目的:建立兔荧光扩增片段长度多态性(fluorescent amplification fragment length polymorphism,fAFLP)分析方法并对其在实验动物科学中应用进行探讨。方法:本实验以5个品种(系)兔为实验材料,对DNA提取、预扩增和选择性扩增等步骤进行...目的:建立兔荧光扩增片段长度多态性(fluorescent amplification fragment length polymorphism,fAFLP)分析方法并对其在实验动物科学中应用进行探讨。方法:本实验以5个品种(系)兔为实验材料,对DNA提取、预扩增和选择性扩增等步骤进行优化并对结果进行检测。结果:5个品种(系)兔的扩增片段数均在120条以上,各品种(系)特异性条带均能找出,5个品种(系)兔能据此进行区分。结论:fAFLP是一种以PCR为基础的DNA指纹技术,为兔的指纹分析提供了一种快速高效的方法,可用于兔的遗传质量检测。展开更多
首次使用R环境中的OneMap软件包,以荷包红鲤抗寒品系(♂)和云南大头鲤(♀)为祖父母本所培育的110个F2个体为作图群体,以荧光扩增片段长度多态性(fluorescent amplification fragment length polymorphism,fAFLP)为主要分子标记,采用远...首次使用R环境中的OneMap软件包,以荷包红鲤抗寒品系(♂)和云南大头鲤(♀)为祖父母本所培育的110个F2个体为作图群体,以荧光扩增片段长度多态性(fluorescent amplification fragment length polymorphism,fAFLP)为主要分子标记,采用远交全同胞家系模型构建鲤的遗传连锁图谱。结果显示,110个F2个体中共产生1513个清晰的fAFLP标记,其中多态性标记911个;另开发多态性的EST标记12个,最后总计923个标记用于构建遗传连锁图谱;采用OneMap软件包构建的遗传图谱含有238个fAFLP标记和8个EST标记分布在50个连锁群上,总图距为2876.64cM,标记间平均间距为14.68cM,图谱覆盖率为66.56%。展开更多
Wheat leaf rust caused by Puccinia triticina, is the most common and widely distributed wheat rust in the world. In order to study the genetic structure of leaf rust population 14 pairs of AFLP and 6 pairs of FAFLP pr...Wheat leaf rust caused by Puccinia triticina, is the most common and widely distributed wheat rust in the world. In order to study the genetic structure of leaf rust population 14 pairs of AFLP and 6 pairs of FAFLP primers evaluated on 86 isolates samples collected in Iran during spring of 2009. Results showed that almost all investigated isolates were genetically different and special pattern of AFLP allele’s that confirm high genetic diversity within leaf rust population was observed. Analyses showed, all provinces were classified into three major groups particularly similar clusters were found between then neighboring provinces. Rust spore can follow the migration pattern in short and long distances to neighbor in provinces. Results indicated that the greatest variability was revealed by 97% of genetic differentiation within leaf rust populations and the lesser variation of 3% was observed between the rust populations. These results suggested that each population was not completely identical and high gene flow has occurred among the leaf rust population of different provinces. The highest differentiation and genetic distance among the Iranian leaf rust populations was detected between leaf rust population in Sistan and Baluchistan and highest similarity was observed between in Ardabil provinces. The high pathogenic variability of leaf rust races in Ardabil and Northern Khorasan may be an indication that these two regions are the center of origin of pathogenic arability. Present study shows that leaf rust population in Iran is highly dynamic and variable.展开更多
文摘目的:建立兔荧光扩增片段长度多态性(fluorescent amplification fragment length polymorphism,fAFLP)分析方法并对其在实验动物科学中应用进行探讨。方法:本实验以5个品种(系)兔为实验材料,对DNA提取、预扩增和选择性扩增等步骤进行优化并对结果进行检测。结果:5个品种(系)兔的扩增片段数均在120条以上,各品种(系)特异性条带均能找出,5个品种(系)兔能据此进行区分。结论:fAFLP是一种以PCR为基础的DNA指纹技术,为兔的指纹分析提供了一种快速高效的方法,可用于兔的遗传质量检测。
文摘首次使用R环境中的OneMap软件包,以荷包红鲤抗寒品系(♂)和云南大头鲤(♀)为祖父母本所培育的110个F2个体为作图群体,以荧光扩增片段长度多态性(fluorescent amplification fragment length polymorphism,fAFLP)为主要分子标记,采用远交全同胞家系模型构建鲤的遗传连锁图谱。结果显示,110个F2个体中共产生1513个清晰的fAFLP标记,其中多态性标记911个;另开发多态性的EST标记12个,最后总计923个标记用于构建遗传连锁图谱;采用OneMap软件包构建的遗传图谱含有238个fAFLP标记和8个EST标记分布在50个连锁群上,总图距为2876.64cM,标记间平均间距为14.68cM,图谱覆盖率为66.56%。
文摘Wheat leaf rust caused by Puccinia triticina, is the most common and widely distributed wheat rust in the world. In order to study the genetic structure of leaf rust population 14 pairs of AFLP and 6 pairs of FAFLP primers evaluated on 86 isolates samples collected in Iran during spring of 2009. Results showed that almost all investigated isolates were genetically different and special pattern of AFLP allele’s that confirm high genetic diversity within leaf rust population was observed. Analyses showed, all provinces were classified into three major groups particularly similar clusters were found between then neighboring provinces. Rust spore can follow the migration pattern in short and long distances to neighbor in provinces. Results indicated that the greatest variability was revealed by 97% of genetic differentiation within leaf rust populations and the lesser variation of 3% was observed between the rust populations. These results suggested that each population was not completely identical and high gene flow has occurred among the leaf rust population of different provinces. The highest differentiation and genetic distance among the Iranian leaf rust populations was detected between leaf rust population in Sistan and Baluchistan and highest similarity was observed between in Ardabil provinces. The high pathogenic variability of leaf rust races in Ardabil and Northern Khorasan may be an indication that these two regions are the center of origin of pathogenic arability. Present study shows that leaf rust population in Iran is highly dynamic and variable.
