Background:Fibroblast activation protein(FAP),a cell surface serine protease,plays roles in tumor invasion and immune regulation.However,there is currently no pan-cancer analysis of FAP.Objective:We aimed to assess th...Background:Fibroblast activation protein(FAP),a cell surface serine protease,plays roles in tumor invasion and immune regulation.However,there is currently no pan-cancer analysis of FAP.Objective:We aimed to assess the pan-cancer expression profile of FAP,its molecular function,and its potential role in head and neck squamous cell carcinoma(HNSC).Methods:We analyzed gene expression,survival status,immune infiltration,and molecular functional pathways of FAP in The Cancer Genome Atlas(TCGA)and Genotype Tissue Expression(GTEx)tumors.Furthermore,to elucidate the role of FAP in HNSC,we performed proliferation,migration,and invasion assays post-FAP overexpression or knock-down.Results:FAP expression was elevated in nine tumor types and was associated with poor survival in eight of them.In the context of immune infiltration,FAP expression negatively correlated with CD8+T-cell infiltration infive tumor types and positively with regulatory T-cell infiltration in four tumor types.Our enrichment analysis highlighted FAP’s involvement in the PI3K-Akt signaling pathway.In HNSC cells,FAP overexpression activated the PI3K-Akt pathway,promoting tumor proliferation,migration,and invasion.Conversely,FAP knockdown showed inhibitory effects.Conclusion:Our study unveils the association of FAP with poor tumor prognosis across multiple cancers and highlights its potential as a therapeutic target in HNSC.展开更多
"电影作为哲学"(Film as Philosophy,简称FAP),在当代电影哲学研究领域引起了广泛的关注与热烈的讨论。该命题力图将(某些)电影与哲学相提并论,主张电影活动即哲学活动。为证明该理论,一方面,FAP支持者试图重新确立"哲学..."电影作为哲学"(Film as Philosophy,简称FAP),在当代电影哲学研究领域引起了广泛的关注与热烈的讨论。该命题力图将(某些)电影与哲学相提并论,主张电影活动即哲学活动。为证明该理论,一方面,FAP支持者试图重新确立"哲学"概念,把电影包括到本真意义的哲学活动中;另一方面,FAP主张者从具体个案研究出发,力图证明电影活动确实可能具有哲学性。围绕FAP的理论争议,具有重要的思想贡献与方法论价值,让我们得以更为深入地审视电影与哲学。展开更多
基金This study was supported in part by grants from the National Natural Science Foundation of China(No.82170972).
文摘Background:Fibroblast activation protein(FAP),a cell surface serine protease,plays roles in tumor invasion and immune regulation.However,there is currently no pan-cancer analysis of FAP.Objective:We aimed to assess the pan-cancer expression profile of FAP,its molecular function,and its potential role in head and neck squamous cell carcinoma(HNSC).Methods:We analyzed gene expression,survival status,immune infiltration,and molecular functional pathways of FAP in The Cancer Genome Atlas(TCGA)and Genotype Tissue Expression(GTEx)tumors.Furthermore,to elucidate the role of FAP in HNSC,we performed proliferation,migration,and invasion assays post-FAP overexpression or knock-down.Results:FAP expression was elevated in nine tumor types and was associated with poor survival in eight of them.In the context of immune infiltration,FAP expression negatively correlated with CD8+T-cell infiltration infive tumor types and positively with regulatory T-cell infiltration in four tumor types.Our enrichment analysis highlighted FAP’s involvement in the PI3K-Akt signaling pathway.In HNSC cells,FAP overexpression activated the PI3K-Akt pathway,promoting tumor proliferation,migration,and invasion.Conversely,FAP knockdown showed inhibitory effects.Conclusion:Our study unveils the association of FAP with poor tumor prognosis across multiple cancers and highlights its potential as a therapeutic target in HNSC.
文摘"电影作为哲学"(Film as Philosophy,简称FAP),在当代电影哲学研究领域引起了广泛的关注与热烈的讨论。该命题力图将(某些)电影与哲学相提并论,主张电影活动即哲学活动。为证明该理论,一方面,FAP支持者试图重新确立"哲学"概念,把电影包括到本真意义的哲学活动中;另一方面,FAP主张者从具体个案研究出发,力图证明电影活动确实可能具有哲学性。围绕FAP的理论争议,具有重要的思想贡献与方法论价值,让我们得以更为深入地审视电影与哲学。
