连续变化法研究Fe(Ⅱ)和Ferrozine的相互作用

Ferrozine,即3-(2-吡啶基)-5,6-双(4-苯磺酸)-1,2,4-三嗪,简写成Fz.作为一种灵敏的Fe光度试剂,广泛用于生物体系和一般材料中Fe的分析.在反应过程中Fe(Ⅱ)和Fz生成FeFz_3.但至今对其相互作用的机理尚不十分清楚.本文应用连续变化法研究它们之间的相互作用.

用ferrozine分光光度法测定Fe^(2+)时干扰因素的排除

分析了用ferrozine分光光度法测定Fe2 + 浓度时硼酸硼酸盐缓冲溶液和共存离子S2 -对结果产生干扰的原因 ,提出了在ferrozine进行显色反应前先用HCl酸化处理来消除测定干扰的方法 ,取得了较满意的Fe2 + 测定结果。

改进Ferrozine法测定溶液中的二价铁、三价铁及总铁

铁元素是自然界中常见的微量元素之一,其在溶液中存在亚铁离子、三价铁离子两种价态。为了准确地定量测定亚铁、三价铁及总铁的含量,探索出一种改进的菲啰嗪(Ferrozine)法。二价铁和Ferrozine反应生成紫色络合物,其在562 nm处有最大吸收峰。利用该特点,可以直接测定二价铁含量,以盐酸羟胺为还原剂,将三价铁还原为二价铁后,即可测定总铁含量,进一步计算出三价铁含量。结果表明:该方法能准确测定游离二价铁及总铁,测定结果和ICP-AES法的结果吻合,同时三价铁计算结果和黄血盐测定三价铁的结果吻合;并且该方法可用于预测分散在水相的四氧化三铁纳米颗粒的铁含量,与ICPAES法相比,校正系数约为0.85。因此该方法适用性广,操作简单而经济,结果准确。

Spectrophotometric Determination of Hydrogen Molecule by Redox Reaction of Ferric and Cupric Using Platinum Catalyzer

Simple methods for the determination of hydrogen molecule in water are developed. These methods are based on the redox reactions between metal ions such as ferric ion and cupric ion with hydrogen molecule in the presence of colloidal platinum. The released ferrous ion was developed with o-phenanthlorine, bathophenanthroline disul fonate and Ferrozine, on the other hand cuprous ion was developed with bathocuproinedisulfonate. In these methods, ferric ion is superior than cupric ion because of its sensitivity and stoichiometric reaction with hydrogen molecule. The hydrogen molecule proved to decompose hydroxy radical released from Fenton reaction by spectrofluorometry.