The human hair follicle, a mini-organ formed with neuroectodermal-meso-dermal interaction, is a complex structure, in the active steady state (anagen) the dermal papilla can be considered as a ball of extracellular ma...The human hair follicle, a mini-organ formed with neuroectodermal-meso-dermal interaction, is a complex structure, in the active steady state (anagen) the dermal papilla can be considered as a ball of extracellular matrix, surrounding specialized fibroblasts. The cross-talk of dermal papilla with neighbouring matrix cells results in the maintenance of hair fibre production. This study aimed to investigate the proliferative potential of the compound TrichotechTM, a phytocomplex obtained from a mixture of essential oils, on cultured human fibroblasts and its ability to modulate the gene expression of FGF-7 and FGF-10. TrichotechTM was shown to enhance fibroblasts proliferation in concentrations of 0.5% to 2.0%, and also increase the percentage of cells in the S/G2/M phases of the cell cycle. TrichotechTM at both 1.0% and 2.0% induced a statistically significant effect on wound healing assay compared to the untreated control. We examined the interaction between cell survival (PI3K/Akt) and mitogenic (Ras/MAPK) signal transduction pathways after TrichotechTM treatment (1.0% and 2.0%) on the fibroblast cell line. TrichotechTM caused phosphorylation of ERK1/2, as well as greater phosphorylation of MEK in comparison with both the untreated control and ERK1/2. PI3K and AKT, however, were not shown to be significantly more phosphorylated following TrichotechTM exposure. To verify the relative expression of mRNA for FGF-7 and FGF-10 genes, a real-time polymerase chain reaction (qPCR) protocol was used. Results show the increase in mRNA expression by fibroblasts after treatment with TrichotechTM. In both concentrations tested, TrichotechTM was found to increase the expression of FGF-7 and FGF-10. Sirius red staining allows for rapid assessment of collagen content, it showed a significant increase in collagen content in treated fibroblasts. Further investigation concerning TrichotechTM could be helpful towards the development of new bioactive phytocomplexes for dermatological and trichological use.展开更多
目的观察复方黄柏液对家兔皮肤破损创口愈合以及对肉芽组织中TGF-β1、VEGF、FGF-7表达的影响。方法 15只家兔随机分为5组,即对照组、京万红组、复方黄柏液0.3、0.2、0.1 m L组,用手术刀在每只家兔耳内侧做长方形切口复制皮肤切除性伤...目的观察复方黄柏液对家兔皮肤破损创口愈合以及对肉芽组织中TGF-β1、VEGF、FGF-7表达的影响。方法 15只家兔随机分为5组,即对照组、京万红组、复方黄柏液0.3、0.2、0.1 m L组,用手术刀在每只家兔耳内侧做长方形切口复制皮肤切除性伤口模型,分别使用复方黄柏液0.3、0.2、0.1 m L及京万红软膏外敷伤口,给药5 d,以肉芽组织覆盖率评价创面愈合情况,并通过免疫组化方法观察创口肉芽组织中TGF-β1、VEGF、FGF-7的表达。结果复方黄柏液可不同程度提高创口肉芽组织覆盖率,使创口肉芽组织中VEGF、FGF-7的表达增加,显著高于对照组(P<0.05,P<0.01)。结论复方黄柏液可明显促进肉芽组织增生,促进皮肤伤口的愈合。展开更多
As the seventh member of Fibroblast Growth Factor （FGF） family, Keratinocyte Growth Factor （KGF or FGF-7） is observed tp mediate epithelial cell proliferation and differentiation in a variety of tissues. In this a...As the seventh member of Fibroblast Growth Factor （FGF） family, Keratinocyte Growth Factor （KGF or FGF-7） is observed tp mediate epithelial cell proliferation and differentiation in a variety of tissues. In this article, such following issues within KGF research were reviewed, as （1） KGF functioning pathways： experimental results demonstrated the paracrine pathway of KGF played main role in mesen- chymal-epithelial interactions whereas KGF itself was under the control of a feedback regulation, autocrine provided KGF alternative functioning way particularly in tumourogenesis; （2） KGF in apoptosis： a few of investigations recently illustrated KGF mediated cell survival was based on its mitogenic function via stimulating cell growth, moreover KGF could inhibit the ROS-induced apoptosis through Nrf-2 pathway; （3） KGF during tumourogenesis： high expression of KGF enhanced progression, motility and invasiveness of tumor cells and various cancers, in company with paracrine loop replaced by autocrine loop, meanwhile KGF clearly played the early signal in the progression of breast cancer; （4） Medical application and administration of KGF： KGF had been successfully used in several preclinical models of radiation and chemotherapy-induced mucositis, and developed into commercial medicine （i.e. Palifermin ）, however more effective delivery systems are still under trial.展开更多
Fibroblast growth factor-2 (FGF-2) is a member of a large family of proteins that bind heparin and heparan sulfate and modulate the function of a wide range of cell types. It has been proved that FGF-2 stimulates the ...Fibroblast growth factor-2 (FGF-2) is a member of a large family of proteins that bind heparin and heparan sulfate and modulate the function of a wide range of cell types. It has been proved that FGF-2 stimulates the growth and development of new blood vessels (angiogenesis) that contribute to the pathogenesis of several diseases (i.e. cancer, atherosclerosis). However, many of the biological activities of FGF-2 have been found to depend on its receptor抯 intrinsic tyrosine kinase activity and second messengers such as the mitogen activated protein kinases. This review will focus on the mechanism of FGF-2/FGFR induced signaling pathway in tumor and human breast cancer.展开更多
文摘The human hair follicle, a mini-organ formed with neuroectodermal-meso-dermal interaction, is a complex structure, in the active steady state (anagen) the dermal papilla can be considered as a ball of extracellular matrix, surrounding specialized fibroblasts. The cross-talk of dermal papilla with neighbouring matrix cells results in the maintenance of hair fibre production. This study aimed to investigate the proliferative potential of the compound TrichotechTM, a phytocomplex obtained from a mixture of essential oils, on cultured human fibroblasts and its ability to modulate the gene expression of FGF-7 and FGF-10. TrichotechTM was shown to enhance fibroblasts proliferation in concentrations of 0.5% to 2.0%, and also increase the percentage of cells in the S/G2/M phases of the cell cycle. TrichotechTM at both 1.0% and 2.0% induced a statistically significant effect on wound healing assay compared to the untreated control. We examined the interaction between cell survival (PI3K/Akt) and mitogenic (Ras/MAPK) signal transduction pathways after TrichotechTM treatment (1.0% and 2.0%) on the fibroblast cell line. TrichotechTM caused phosphorylation of ERK1/2, as well as greater phosphorylation of MEK in comparison with both the untreated control and ERK1/2. PI3K and AKT, however, were not shown to be significantly more phosphorylated following TrichotechTM exposure. To verify the relative expression of mRNA for FGF-7 and FGF-10 genes, a real-time polymerase chain reaction (qPCR) protocol was used. Results show the increase in mRNA expression by fibroblasts after treatment with TrichotechTM. In both concentrations tested, TrichotechTM was found to increase the expression of FGF-7 and FGF-10. Sirius red staining allows for rapid assessment of collagen content, it showed a significant increase in collagen content in treated fibroblasts. Further investigation concerning TrichotechTM could be helpful towards the development of new bioactive phytocomplexes for dermatological and trichological use.
文摘目的观察复方黄柏液对家兔皮肤破损创口愈合以及对肉芽组织中TGF-β1、VEGF、FGF-7表达的影响。方法 15只家兔随机分为5组,即对照组、京万红组、复方黄柏液0.3、0.2、0.1 m L组,用手术刀在每只家兔耳内侧做长方形切口复制皮肤切除性伤口模型,分别使用复方黄柏液0.3、0.2、0.1 m L及京万红软膏外敷伤口,给药5 d,以肉芽组织覆盖率评价创面愈合情况,并通过免疫组化方法观察创口肉芽组织中TGF-β1、VEGF、FGF-7的表达。结果复方黄柏液可不同程度提高创口肉芽组织覆盖率,使创口肉芽组织中VEGF、FGF-7的表达增加,显著高于对照组(P<0.05,P<0.01)。结论复方黄柏液可明显促进肉芽组织增生,促进皮肤伤口的愈合。
基金This work was supported by the scientific scholarship of NEFU to D. ZHENG., the EYTIF fund of NEFU to X.LIU, and partial sup-ported by the grant of the Ministration of Education (020-413229) to D. ZHENG
文摘As the seventh member of Fibroblast Growth Factor （FGF） family, Keratinocyte Growth Factor （KGF or FGF-7） is observed tp mediate epithelial cell proliferation and differentiation in a variety of tissues. In this article, such following issues within KGF research were reviewed, as （1） KGF functioning pathways： experimental results demonstrated the paracrine pathway of KGF played main role in mesen- chymal-epithelial interactions whereas KGF itself was under the control of a feedback regulation, autocrine provided KGF alternative functioning way particularly in tumourogenesis; （2） KGF in apoptosis： a few of investigations recently illustrated KGF mediated cell survival was based on its mitogenic function via stimulating cell growth, moreover KGF could inhibit the ROS-induced apoptosis through Nrf-2 pathway; （3） KGF during tumourogenesis： high expression of KGF enhanced progression, motility and invasiveness of tumor cells and various cancers, in company with paracrine loop replaced by autocrine loop, meanwhile KGF clearly played the early signal in the progression of breast cancer; （4） Medical application and administration of KGF： KGF had been successfully used in several preclinical models of radiation and chemotherapy-induced mucositis, and developed into commercial medicine （i.e. Palifermin ）, however more effective delivery systems are still under trial.
基金supported by the National Natural Science Foundation of China(Grant Nos.30100092 and 30270666)
文摘Fibroblast growth factor-2 (FGF-2) is a member of a large family of proteins that bind heparin and heparan sulfate and modulate the function of a wide range of cell types. It has been proved that FGF-2 stimulates the growth and development of new blood vessels (angiogenesis) that contribute to the pathogenesis of several diseases (i.e. cancer, atherosclerosis). However, many of the biological activities of FGF-2 have been found to depend on its receptor抯 intrinsic tyrosine kinase activity and second messengers such as the mitogen activated protein kinases. This review will focus on the mechanism of FGF-2/FGFR induced signaling pathway in tumor and human breast cancer.
