The investigation of fingerprint of Yubaizhi was performed by high-performance liquid chromatography （HPLC）. Chromatographic conditions were： the mobile phase was the mixture of acetonitrile-water （43：57, v/v）; ...The investigation of fingerprint of Yubaizhi was performed by high-performance liquid chromatography （HPLC）. Chromatographic conditions were： the mobile phase was the mixture of acetonitrile-water （43：57, v/v）; the flow rate was 1 mL/min; the column temperature was 40℃ ; the detection wavelength was 254 nm. The stability, precision and reproducibility were performed according to ＂Specification of Fingerprint of Injection of Chinese Traditional Medicine （temporarily）＂ regulated by State Food and Drug Administration（SFDA）. The HPLC fingerprints of Yubaizhi from various locations were well constant.展开更多
目的建立富马酸卢帕他定含量测定方法。方法采用高效液相色谱法(HPLC)法,色谱柱:Agilent Eclipse Plus C18(4.6 mm×150 mm,3.5μm);流动相:20 mmol/L乙酸铵(pH=5.0)为流动相A,甲醇为流动相B,进行梯度洗脱,柱温30℃,流速1.0 m L...目的建立富马酸卢帕他定含量测定方法。方法采用高效液相色谱法(HPLC)法,色谱柱:Agilent Eclipse Plus C18(4.6 mm×150 mm,3.5μm);流动相:20 mmol/L乙酸铵(pH=5.0)为流动相A,甲醇为流动相B,进行梯度洗脱,柱温30℃,流速1.0 m L·min^(-1),检测波长242 nm。结果能够准确测定富马酸卢帕他定含量,供试品及对照品溶液在24 h内稳定;精密度RSD为0.45%,重复性RSD为0.12%,中间精密度RSD为0.51%;富马酸卢帕他定浓度在50~200μg·m L^(-1)范围内线性关系良好;平均回收率分别为100.26%(RSD=0.13%)、99.65%(RSD=0.44%)、100.12%(RSD=0.41%);改变柱温、流速等条件,该方法耐用性均良好。结论所建立的方法专属性强、耐用性好、准确可靠。展开更多
文摘The investigation of fingerprint of Yubaizhi was performed by high-performance liquid chromatography （HPLC）. Chromatographic conditions were： the mobile phase was the mixture of acetonitrile-water （43：57, v/v）; the flow rate was 1 mL/min; the column temperature was 40℃ ; the detection wavelength was 254 nm. The stability, precision and reproducibility were performed according to ＂Specification of Fingerprint of Injection of Chinese Traditional Medicine （temporarily）＂ regulated by State Food and Drug Administration（SFDA）. The HPLC fingerprints of Yubaizhi from various locations were well constant.
