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环孢素A体外抗FITC-ConA标记的曼氏血吸虫童虫的作用 被引量:1
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作者 柳建发 Kusel JR 《中国人兽共患病学报》 CAS CSCD 北大核心 2006年第6期568-570,共3页
目的探讨环孢素A体外抗FITC ConA标记的曼氏血吸虫童虫的作用以及虫体表膜通透性。方法人工制备曼氏血吸虫童虫,使用FITC ConA进行标记,环孢素A体外作用,荧光显微镜下观察并摄照。结果童虫损伤或死亡,虫体逐渐从淡蓝色变成亮蓝色,虫体... 目的探讨环孢素A体外抗FITC ConA标记的曼氏血吸虫童虫的作用以及虫体表膜通透性。方法人工制备曼氏血吸虫童虫,使用FITC ConA进行标记,环孢素A体外作用,荧光显微镜下观察并摄照。结果童虫损伤或死亡,虫体逐渐从淡蓝色变成亮蓝色,虫体可见多个亮蓝色小点,虫体表面损伤。结论环孢素A能增加童虫表膜流动性。 展开更多
关键词 环孢素A 曼氏血吸虫 童虫 fitc—cona
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In Vitro Fertilization in Maize and Tobacco: The Fusion Patterns During Sexual Cell Fusion 被引量:1
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作者 孙蒙祥 杨弘远 Mauro CRESTI 《Acta Botanica Sinica》 CSCD 2002年第10期1142-1145,共4页
Large scale of sexual cell fusion was carried out in order to observe the fusion pattern during in vitro fertilization with special interest in the influence of cell volume on the membrane behavior. Three patterns cou... Large scale of sexual cell fusion was carried out in order to observe the fusion pattern during in vitro fertilization with special interest in the influence of cell volume on the membrane behavior. Three patterns could be recognized in sexual cell fusion, which was supported by fluorescent microscopy with the aid of video enhanced system and cooled CCD. It was found that the fusion pattern and cell membrane behavior were related to the cell volume ratio of two fusing cells. The results reported here might be useful for interpreting and evaluating data from in vitro fertilization experiments and for explaining male cytoplasm exclusion during fertilization. 展开更多
关键词 in vitro fertilization MAIZE TOBACCO cell volume fusion pattern fitc-Con A
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