Kinesin superfamily of microtubule- based motor orchestrates a variety of cellular proc- esses. Recent availability of mammalian genomes has enabled analyses of kinesins on the whole ge- nome. Here we present a novel ...Kinesin superfamily of microtubule- based motor orchestrates a variety of cellular proc- esses. Recent availability of mammalian genomes has enabled analyses of kinesins on the whole ge- nome. Here we present a novel full-length kinesin prediction program (FKPP) for mammalian kinesin gene discovery based on a comparative genomics approach. Contrary to previous predictions of 94 kinesins, we identify a total of 134 potentially kinesin genes from mammalian genomes, including 45 from mouse, 45 from rat and 44 from human. In addition, FKPP synthesizes 25 potentially full-length mam- malian kinesins based on the partial sequences in the database. Surprisingly, FKPP reveals that full-length human CENP-E contains 2701 aa rather than 2663 aa in the database. Experimentation using sequence specific antibody and cDNA sequencing of human CENP-E validates the accuracy of FKPP. Given the remarkable computing efficiency and accuracy of FKPP, we reclassify the mammalian kinesin super- family. Since current databases contain many in- complete sequences, FKPP may provide a novel approach for molecular delineation of kinesins and other protein families.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.39925018,30121001,30270293&90508002)the Chinese Academy of Sciences(Grant No.KSCX2-2-01)+3 种基金the Chinese 973 Project(Grant No.2002CB713700)the Chinese 863 Project(Grant No.2001AA215331)Chinese Minister of Education(Grant No.20020358051)American Cancer Society(Grant No.RPG-99-173-01).
文摘Kinesin superfamily of microtubule- based motor orchestrates a variety of cellular proc- esses. Recent availability of mammalian genomes has enabled analyses of kinesins on the whole ge- nome. Here we present a novel full-length kinesin prediction program (FKPP) for mammalian kinesin gene discovery based on a comparative genomics approach. Contrary to previous predictions of 94 kinesins, we identify a total of 134 potentially kinesin genes from mammalian genomes, including 45 from mouse, 45 from rat and 44 from human. In addition, FKPP synthesizes 25 potentially full-length mam- malian kinesins based on the partial sequences in the database. Surprisingly, FKPP reveals that full-length human CENP-E contains 2701 aa rather than 2663 aa in the database. Experimentation using sequence specific antibody and cDNA sequencing of human CENP-E validates the accuracy of FKPP. Given the remarkable computing efficiency and accuracy of FKPP, we reclassify the mammalian kinesin super- family. Since current databases contain many in- complete sequences, FKPP may provide a novel approach for molecular delineation of kinesins and other protein families.
