为了实现分布反馈式光纤激光传感器(DFB FL)大动态范围、稳定解调,建立了基于3×3耦合器的迈克尔逊干涉仪解调系统。对该系统所采用的对称解调算法(NPS)和反正切解调算法进行了深入研究。首先,介绍了基于3×3耦合器解调算法的...为了实现分布反馈式光纤激光传感器(DFB FL)大动态范围、稳定解调,建立了基于3×3耦合器的迈克尔逊干涉仪解调系统。对该系统所采用的对称解调算法(NPS)和反正切解调算法进行了深入研究。首先,介绍了基于3×3耦合器解调算法的原理及耦合器不对称时的调校方法。接着,对干涉仪所需最小非平衡路径长度的选取与系统强度噪声、激光器频率噪声的关系进行了分析。最后,针对NPS算法与反正切算法最大可解调信号幅度进行了分析对比,并研究了微分器对对称解调方法解调范围的影响。实验结果表明:NPS算法动态范围高于反正切算法,微分器的幅频特性不理想会减小解调动态范围。在采样频率为125 k Hz、信号频率为1 k Hz、干涉仪非平衡路径为100 m时,NPS算法与反正切算法的动态范围分别达到96 d B和90 d B。用解调前调校的方法,基于3×3耦合的解调方法动态范围大,能够实现稳定解调,满足工程应用要求。展开更多
Mycobacterium tuberculosis 6-kDa early secretory antigenic target （ESAT-6） is a dominant target antigen for cell-mediated immunity in the early phase of tuberculosis. The fms-like tyrosine kinase 3 ligand （FL） tha...Mycobacterium tuberculosis 6-kDa early secretory antigenic target （ESAT-6） is a dominant target antigen for cell-mediated immunity in the early phase of tuberculosis. The fms-like tyrosine kinase 3 ligand （FL） that induces potent immune response has been used as an adjuvant in vaccine development. In this study, a new recombinant plasmid （plRES-epitope-peptides-FL） encoding three T cell epitopes of ESAT-6 and FL was constructed, and the immunogenicity of the DNA vaccine was assessed in C57BL/6 mice immunized with the plasmid DNA vaccine. Additionally, a strategy of intramuscular injection with the DNA vaccine （prime） and intranasal administration of the epitope peptides （boost） was employed to induce higher immune reaction of the mice. The results showed that mice vaccinated with the recombinant plasmid DNA vaccine and boosted with the peptides not only increased the levels of Thl cytokines （IFN-γ and IL-12）, the number of IFN-γ＋ T cells and activities of cytotoxic T lymphocytes as well as IgG, but also enhanced protection against Mycobacterium tuberculosis challenge. In conclusion, these data indicate that the novel recombinant plRES-epitope-peptides-FL plasmid is a useful DNA vaccine for pre- venting Mycobacterium tuberculosis infection.展开更多
Ten novel N-acyl-3-（3-pyridyl）-5-aryl-pyrazoles were synthesized by Claisen condensation of the aryl methyl ketones with ethyl nicotinate, the cyclization with hydrazine hydrate and the N-acylation with acyl chlorid...Ten novel N-acyl-3-（3-pyridyl）-5-aryl-pyrazoles were synthesized by Claisen condensation of the aryl methyl ketones with ethyl nicotinate, the cyclization with hydrazine hydrate and the N-acylation with acyl chloride in turn. The structures of all the compounds synthesized were confirmed by means of Fourier transform infrared（FTIR）, IH NMR, mass spectroscopy and elemental analysis. The biological activities of the title compounds were examined by disc diffusion method against Escherichia coil Staphylococcus aureus, Pyricularia oryzae and Rhizoctnia solani. All the N-acyl-3-（3-pyridyl）-5-aryl-pyrazoles exhibited a certain degree of antibacterial and antifungal activities. Comparatively, compounds 3c and 3d exhibited much significant antibacterial and antifungal activities than the other pyrazole derivatives.展开更多
文摘为了实现分布反馈式光纤激光传感器(DFB FL)大动态范围、稳定解调,建立了基于3×3耦合器的迈克尔逊干涉仪解调系统。对该系统所采用的对称解调算法(NPS)和反正切解调算法进行了深入研究。首先,介绍了基于3×3耦合器解调算法的原理及耦合器不对称时的调校方法。接着,对干涉仪所需最小非平衡路径长度的选取与系统强度噪声、激光器频率噪声的关系进行了分析。最后,针对NPS算法与反正切算法最大可解调信号幅度进行了分析对比,并研究了微分器对对称解调方法解调范围的影响。实验结果表明:NPS算法动态范围高于反正切算法,微分器的幅频特性不理想会减小解调动态范围。在采样频率为125 k Hz、信号频率为1 k Hz、干涉仪非平衡路径为100 m时,NPS算法与反正切算法的动态范围分别达到96 d B和90 d B。用解调前调校的方法,基于3×3耦合的解调方法动态范围大,能够实现稳定解调,满足工程应用要求。
基金supported by the Natural Scientific Fund (09KJA310002DG216D50162011NJMU263 and 11JC005) of China
文摘Mycobacterium tuberculosis 6-kDa early secretory antigenic target （ESAT-6） is a dominant target antigen for cell-mediated immunity in the early phase of tuberculosis. The fms-like tyrosine kinase 3 ligand （FL） that induces potent immune response has been used as an adjuvant in vaccine development. In this study, a new recombinant plasmid （plRES-epitope-peptides-FL） encoding three T cell epitopes of ESAT-6 and FL was constructed, and the immunogenicity of the DNA vaccine was assessed in C57BL/6 mice immunized with the plasmid DNA vaccine. Additionally, a strategy of intramuscular injection with the DNA vaccine （prime） and intranasal administration of the epitope peptides （boost） was employed to induce higher immune reaction of the mice. The results showed that mice vaccinated with the recombinant plasmid DNA vaccine and boosted with the peptides not only increased the levels of Thl cytokines （IFN-γ and IL-12）, the number of IFN-γ＋ T cells and activities of cytotoxic T lymphocytes as well as IgG, but also enhanced protection against Mycobacterium tuberculosis challenge. In conclusion, these data indicate that the novel recombinant plRES-epitope-peptides-FL plasmid is a useful DNA vaccine for pre- venting Mycobacterium tuberculosis infection.
基金Supported by the National Natural Science Foundation of China（No.21273065）.
文摘Ten novel N-acyl-3-（3-pyridyl）-5-aryl-pyrazoles were synthesized by Claisen condensation of the aryl methyl ketones with ethyl nicotinate, the cyclization with hydrazine hydrate and the N-acylation with acyl chloride in turn. The structures of all the compounds synthesized were confirmed by means of Fourier transform infrared（FTIR）, IH NMR, mass spectroscopy and elemental analysis. The biological activities of the title compounds were examined by disc diffusion method against Escherichia coil Staphylococcus aureus, Pyricularia oryzae and Rhizoctnia solani. All the N-acyl-3-（3-pyridyl）-5-aryl-pyrazoles exhibited a certain degree of antibacterial and antifungal activities. Comparatively, compounds 3c and 3d exhibited much significant antibacterial and antifungal activities than the other pyrazole derivatives.
