On June 282022 Sweden and Finland were invited to join NATO.During a symbolic ceremony at the NATO Madrid Summit,all the central actors gathered in front of the cameras,as if to replicate a“family photo”in mini form...On June 282022 Sweden and Finland were invited to join NATO.During a symbolic ceremony at the NATO Madrid Summit,all the central actors gathered in front of the cameras,as if to replicate a“family photo”in mini format:representatives from Sweden,Finland,and Turkey,as well as Secretary General Stoltenberg.The atmosphere was mixed:tense because of the dramatic backdrop of the Summit,relief because of the speedy process that had taken the process further.It was finally happening:Sweden and Finland would join the Atlantic Pact.But how did they get there?Over a few hectic months,two countries strongly associated with neutrality had moved their policies from emphasizing continuity to altering the very foundations of their security.Not only NATO critics were taken by surprise.Many NATO supporters were astonished by the rapid pace and the turnaround of the Swedish Social Democratic party.The relatively quick turnaround in Stockholm also meant that questions about the future and the consequences of NATO membership were postponed,not least because the Swedish election campaign put a lid on debate about strategic consequences:what journey awaits in the future and what strategic consequences does a future NATO membership have?This article discusses these questions in the belief that the temporal perspectives are connected:Sweden’s historical collaboration with NATO had significance for the country’s partner relationship,and in turn this influenced how Sweden became a member and what challenges the country faces together with its allies on a new northern flank in Europe.展开更多
Mutator transposable element (Mu) has been used as an effective tool to clone maize (Zea mays L.) genes. One opaque endosperm mutant (miol6) was identified in a pool of Mu inserted mutants. A modified method, te...Mutator transposable element (Mu) has been used as an effective tool to clone maize (Zea mays L.) genes. One opaque endosperm mutant (miol6) was identified in a pool of Mu inserted mutants. A modified method, termed the double selected amplification of insertion flanking fragments (DSAIFF), was employed to isolate the Mu flanking fragments (MFFs) of miol6. The target site duplications (TSDs) isolated from the Msp I and Mse I digested MFFs had a same 9-bp sequence and were confirmed to be the flanking sequence of one identically inserted gene. Co-segregation analysis suggested that the MFFs were associated with the mutant opaque endosperm, and miol6 was mapped in silico onto the physical position ranged from 229 965 021 to 229 965 409 bp of the maize chromosome 4.09 bin. The full-length cDNA of the wild-type gene was obtained by an RT-PCR primer-scanning technique, and Mio16 was found to putatively encode a homolog of the Arabidopsis MAP3K delta-1 protein kinase. RT-PCR result the mRNA expression of miol6 region anchored by primers Mu20 and af276 was not interrupted by Mu insertion. Further researches will be done to elucidate how the expression of miol6 is alternated by Mu insertion.展开更多
Transposable elements have been utilized as mutagens to create mutant libraries for functional genomics. Isolation of genomic segments flanking the insertion Mutator (Mu) is a key step in insertion mutagenesis studi...Transposable elements have been utilized as mutagens to create mutant libraries for functional genomics. Isolation of genomic segments flanking the insertion Mutator (Mu) is a key step in insertion mutagenesis studies. Herein, we adopted a modified AFLP method to identify and isolate Mu-flanking fragments from maize. The method consists of the following steps: 1) double-digestion of genomic DNA with Bgl II/Msp I and ligation of digested fragments to the Bgl II- and Msp I-adaptors; 2) enrichment of a subset of Bgl II/Msp I fragments followed by selective amplification of the Mu-flanking fragments; 3) simultaneous display of AFLP bands derived from the flanking regions for both insert and native Mu transposons; 4) identification and isolation of AFLP bands resulting from Mu insertions by comparing the banding profiles between Mu-induced mutants and their parental lines; and 5) confirmation of flanking fragments related to these Mu insertions. Using this approach, we have isolated flanking fragment(s) resulting from Mu insertion for every Mu-induced mutant, and one such fragment, M196-FF, is found to contain a partial sequence of the DNA topoisomerase I gene Topl. Moreover, the modified AFLP method including all restriction enzymes, adaptors and primers has been optimized in this study. The modified AFLP method has been proved to be simple and efficient in the isolation of Mu-flanking fragments and will find its usefulness in the functional genomics of maize.展开更多
This study focuses on the trace and rare earth elements(REE)geochemistry of the Nkporo and Ekenkpon Shales of the Calabar Flank.The main aim is to infer their depositional environment and the degree of their metal enr...This study focuses on the trace and rare earth elements(REE)geochemistry of the Nkporo and Ekenkpon Shales of the Calabar Flank.The main aim is to infer their depositional environment and the degree of their metal enrichment.The shale samples were analyzed using inductively coupled plasma mass spectrometry.The results indicated that the mean concentrations of K,Na,and Fe in Nkporo and Ekenkpon Shales are 1.45,0.4,and 4.17 wt%,and 1.11,0.44,and 5.42 wt%;respectively.The Nkporo Shale is enriched with the following trace elements;P>Mn>Sr>Ba>Zn>Ce>Rb>Zr>V>Cr>Ni and depleted in the following trace elements;Ta>Ge>Sb>Bi>Cd>Ag>Te>In>Hg.While the Ekenkpon Shale is enriched with the following trace elements;P>Mn>Ba>Sr>V>Ce>Zr>Rb>Cr>Zn>Ni and depleted in;Sb>Ge>Bi>Ag>Ce>Te>In>Hg.The concentration of redox-sensitive elements such as V,Ni,Mo,U,Cu,Cr,Re,Cd,Sb,Ti,Mn,and their ratio V/Mo and U/Mo in the black and grey shale samples show different patterns.The REE obtained from the Nkporo and Ekenkpon Shales were PAAS normalized.The Nkporo Shale showed a slightly flat light rare-earth element(LREE),middle rare-earth element(MREE),and heavy rare earth element(HREE)pattern enrichment.Ce/Ce*ranges from 0.95 to 1.09 in Nkporo Shale and 0.67 to 1.40 in Ekenkpon Shale.The Ekenkpon Shale showed a slight LREE,MREE enrichment,and depleted HREE patterns.The Mn contents and U/Mo ratio in Nkporo and Ekenkpon Shales suggests a poor oxygen transitional environment.The V/Mo and V/(V+Ni)ratios indicated that the Nkporo shales were deposited in an anoxic to suboxic conditions and Ekenkpon shales were also deposited under an anoxic to suboxic conditions.The V/Ni ratio indicated that the organic matter in the Nkporo shale is terrigenous while that of the Ekenkpon shales are both terrigenous and marine in origin.展开更多
Presents the division of non developable ruled surface into divided small areas and flank milling in these divided areas to improve machining efficiency and machined surface quality by controlling the machining error ...Presents the division of non developable ruled surface into divided small areas and flank milling in these divided areas to improve machining efficiency and machined surface quality by controlling the machining error for each area, and the algorithms developed for generation of tool path and calculation of errors, and concludes from computer simulation results that the algorithms are correct.展开更多
Background: Soft song is a low-amplitude song produced by many birds. Recent studies have confirmed that soft song is an aggressive signal. For example, the Brownish-flanked Bush Warblers Cettia fortipes use soft song...Background: Soft song is a low-amplitude song produced by many birds. Recent studies have confirmed that soft song is an aggressive signal. For example, the Brownish-flanked Bush Warblers Cettia fortipes use soft song in male-male conflicts, particularly prior to attacks. Although stable signaling systems require that signals be honest on average,models predict that cheating is an acceptable strategy for some individuals or in some contexts.Methods: This study aimed to test the reliability of soft song as an aggressive signal in the brownish-flanked bush warbler. We used mounted specimens accompanied by broadcast songs or soft songs to simulate a male attempting to invade an existing territory.Results: We found the mounted specimen that coupled playback of soft songs suffered more and quicker attacks from the territory owner and that the relationship between soft song and subsequent attack in the territory owner was far from perfect. We observed territory owners that both over-signaled(i.e., produced soft song but did not attack) and under-signaled(i.e., attacked without producing soft song). Under-signaling territory owners were relatively more commonly than were over-signaling territory owners, particularly in simulated intrusion that coupled playback of soft song with a mount specimen.Conclusions: We discuss the cost of producing soft song and the potential benefit of the unreliable use of soft song and propose a new hypothesis for under-signaling with soft song; i.e., under-signaling territory owners might benefit from taking the initiative in fights.展开更多
A volcano can be defined as a complex system, not least for the hidden clues related to its internal nature. Innovative models grounded in the Artificial Sciences, have been proposed for a novel pattern recognition an...A volcano can be defined as a complex system, not least for the hidden clues related to its internal nature. Innovative models grounded in the Artificial Sciences, have been proposed for a novel pattern recognition analysis at Mt. Etna volcano. The reference monitoring dataset dealt with real data of 28 parameters collected between January 2001 and April 2005, during which the volcano underwent the July-August 2001, October 2002-January 2003 and September 2004-April 2005 flank eruptions. There were 301 eruptive days out of an overall number of 1581 investigated days. The analysis involved successive steps. First, the TWIST algorithm was used to select the most predictive attributes associated with the flank eruption target. During his work, the algorithm TWIST selected 11 characteristics of the input vector: among them SO<sub>2</sub> and CO<sub>2</sub> emissions, and also many other attributes whose linear correlation with the target was very low. A 5 × 2 Cross Validation protocol estimated the sensitivity and specificity of pattern recognition algorithms. Finally, different classification algorithms have been compared to understand if this pattern recognition task may have suitable results and which algorithm performs best. Best results (higher than 97% accuracy) have been obtained after performing advanced Artificial Neural Networks, with a sensitivity and specificity estimates over 97% and 98%, respectively. The present analysis highlights that a suitable monitoring dataset inferred hidden information about volcanic phenomena, whose highly non-linear processes are enhanced.展开更多
Objective: To analyze the genomic structure of SNC6, a progesterone-receptor associated protein gene and its regulatory elements in its 5'-flanking region. Methods: Genomic sequence from C, enllank database ( acce...Objective: To analyze the genomic structure of SNC6, a progesterone-receptor associated protein gene and its regulatory elements in its 5'-flanking region. Methods: Genomic sequence from C, enllank database ( accession number: Z98048) covering the whole SNC6 gene was used to analyze the genonfic stnmture of SNC6 and design primers for PCR amplification of its 5'-flanking region. A 1894 bp fragrnem of the 5’-flanking region ( - 1814 to + 75) was cloned by PCR using genomic DNA from a healthy donor peripheral blood lymphocyte as template. This fragment, as well as 3 shorter derivative fragments ( 1423 bp, 632 bp and 416bp, which correspond to - 1344 to + 75, - 552 to + 75 and - 337 to + 75 respectively), were subeloned into pGL2 series luciferase reporter vectors. These constructs were introduced into colorectal cancer cell line SW620 for transient expression of reporter gene and luefferase activities were measured. Results: The genomic structure analysis showed there are 12 exons for SNC6 gene, which spans 32017 bp (nt71529 to nt39513 in Z98048 sequence). All tmnsfected SW620 cells with the above 5-flanking region-containing constructs showed lueiferase activities. The highest lueiferase activities were measured in transfected cells with vectors containing 1894 bp fragments, and the lowest luefferase activities were measured in tmnsfected cells with vectors containing 416 bp fragments, lmeiferase activities were higher in transfected cells with vectors containing 632 bp fragments than that in tmnsfected cells with vectors containing 1423 bp fragments. Conclusion: The basle tran-scription-promoting element (promoter) for SNC6 expression resides between 0 to - 337, and two transcription-enhancing dements (enhancer) resides between - 337 to - 552 and - 1344 to - 1814, whereas one transcription-inhibiting element (silencer) exists between -552 to - 1344.展开更多
Behavioral studies have shown that flank glands are involved in chemical communication in golden hamstersMesocricetus auratus but little chemical analysis has been conducted on volatiles arising from these glands. Usi...Behavioral studies have shown that flank glands are involved in chemical communication in golden hamstersMesocricetus auratus but little chemical analysis has been conducted on volatiles arising from these glands. Using gas chromatography-mass spectrometry, we detected compounds from the flank glands of males, only eight of which were also produced infemales. Based on these chemical data we performed a number of further experiments. By manipulating light we found that malesexposed to short-photoperiods developed smaller flank glands than those exposed to long-photoperiods. Six flank gland volatilesreduced in relative abundance, which possibly coded for reproductive status of males of this seasonally breeding hamster species.Through dyadic encounters, we were able to induce the formation of dominant-subordinate relationships and show that two glandularcompounds became high in relative abundance and may function as dominance pheromones. Castration eliminated allmale-specific compounds resulting from flank glands, but bilateral ovariectomies only affected one compound in females. Oncethese ovariectomized females were treated with testosterone, their glandular compounds resembled those of males, suggestingthese compounds are under the main control of androgen. Two female putative pheromones, tetradecanoic acid and hexadecanoicacid, were used in binary choice tests and were both found to attract males over females. Applying a solution of these pheromonecompounds to adult males also suppressed their agonistic展开更多
Three T-DNA insertional embryonic lethal mutants from NASC (The Nottingham Arabidopsis Stock Center)were first checked with their segregation ratio of abortive and normal seeds and the copy number of T-DNA insertion. ...Three T-DNA insertional embryonic lethal mutants from NASC (The Nottingham Arabidopsis Stock Center)were first checked with their segregation ratio of abortive and normal seeds and the copy number of T-DNA insertion. The N4081 mutant has a segregation ratio of 1:3.04in average and one T-DNA insertion site according to our assay It was therefore chosen for further analysis. To isolate the joint fragment of T-DNA and plan DNA, the plasmid rescue technique waJs used. pEL-7, one of plasmids from left border of T-DNA, which contained pBR322 was selected from ampicillin plate. The T-DNA fragment of pEL-7 was checked by restriction enzyme analysis and Southern Blot. Restriction analysis confirmed the presence of known sites of EcoRI, PstI and PvuII on it.For confirming the presence of flanking plant DNA in this plasmid, pEL-7 DNA was labeled and hybridized with wild type and mutant plant DNA. The Southern Blot indicated the hybridization band in both of them. Furthermore, the junction of T-DNA/plant DNA was subcloned into bluescript SK+ and sequenced by Applied Biosystem 373A Sequencer. The results showed the 822 bp fragment contained a 274 bp sequence, which is 99.6%homolog (273bp/274 bp) to Ti plasmid pTi 15955 DNA.Ten bp of left 25 bp border repeat were also found in the juction of T-DNA and Plant DNA.Taken together, pEL-7 should contain a joint fragment of T-DNA and flanking plant DNA. This plasmid DNA could be used for the isolation of plant gene, which will be helpful to elucidate the relationship between gene function and plant embryo development.展开更多
The tool flank begins to wear out as soon as cutting process proceeds. Cutting parameters such as cutting forces and cutting temperature will vary with increasing degree of flank wear. In order to reveal the relations...The tool flank begins to wear out as soon as cutting process proceeds. Cutting parameters such as cutting forces and cutting temperature will vary with increasing degree of flank wear. In order to reveal the relationship between them, the theoretical situations of cutting process were analyzed considering the tool flank wear effect. The variation rules of cutting force, residual stress and temperature distributions along with the tool flank wear were analyzed comparing with the sharp tool tip. Through FEM simulation method, affections of the tool flank wear value VB on cutting forces, residual stress and temperature distributions were analyzed. A special result in this simulation is that the thrust force is more sensitive to tool flank wear, which can be used as a recognition method of tool condition monitoring. The FEM simulation analysis result agrees well with the experimental measuring data in public literatures and some experiments made also by the authors.展开更多
Ruled surfaces found in engineering parts are often blended with a constraint surface,like the blade surface and hub surface of a centrifugal impeller.It is significant to accurately machine these ruled surfaces in fl...Ruled surfaces found in engineering parts are often blended with a constraint surface,like the blade surface and hub surface of a centrifugal impeller.It is significant to accurately machine these ruled surfaces in flank milling with interference-free and fairing tool path,while current models in fulfilling these goals are complex and rare.In this paper,a tool path planning method with optimal cutter locations(CLs)is proposed for 5-axis flank milling of ruled surfaces under multiple geometric constraints.To be specific,a concise three-point contact tool positioning model is firstly developed for a cylindrical cutter.Different tool orientations arise when varying the three contact positions and a tool orientation pool with acceptable cutter-surface deviation is constructed using a meta-heuristic algorithm.Fairing angular curves are derived from candidates in this pool,and then curve registration for cutter tip point on each determined tool axis is performed in respect of interference avoidance and geometric smoothness.On this basis,an adaptive interval determination model is developed for deviation control of interpolated cutter locations.This model is designed to be independent of the CL optimization process so that multiple CLs can be planned simultaneously with parallel computing technique.Finally,tests are performed on representative surfaces and the results show the method has advantages over previous meta-heuristic tool path planning approaches in both machining accuracy and computation time,and receives the best comprehensive performance compared to other multi-constrained methods when machining an impeller.展开更多
Magnesium alloys have many advantages as lightweight materials for engineering applications,especially in the fields of automotive and aerospace.They undergo extensive cutting or machining while making products out of...Magnesium alloys have many advantages as lightweight materials for engineering applications,especially in the fields of automotive and aerospace.They undergo extensive cutting or machining while making products out of them.Dry cutting,a sustainable machining method,causes more friction and adhesion at the tool-chip interface.One of the promising solutions to this problem is cutting tool surface texturing,which can reduce tool wear and friction in dry cutting and improve machining performance.This paper aims to investigate the impact of dimple textures(made on the flank face of cutting inserts)on tool wear and chip morphology in the dry machining of AZ31B magnesium alloy.The results show that the cutting speed was the most significant factor affecting tool flank wear,followed by feed rate and cutting depth.The tool wear mechanism was examined using scanning electron microscope(SEM)images and energy dispersive X-ray spectroscopy(EDS)analysis reports,which showed that at low cutting speed,the main wear mechanism was abrasion,while at high speed,it was adhesion.The chips are discontinuous at low cutting speeds,while continuous at high cutting speeds.The dimple textured flank face cutting tools facilitate the dry machining of AZ31B magnesium alloy and contribute to ecological benefits.展开更多
In this paper, optimum positioning of cylindrical cutter for five-axis flank milling of non-developable ruled surface is addressed from the perspective of surface approximation. Based on the developed interchangeabili...In this paper, optimum positioning of cylindrical cutter for five-axis flank milling of non-developable ruled surface is addressed from the perspective of surface approximation. Based on the developed interchangeability principle, global optimization of the five-axis tool path is modeled as approximation of the tool envelope surface to the data points on the design surface following the minimum zone criterion recommended by ANSI and ISO standards for tolerance evaluation. By using the signed point-to-surface distance function, tool path plannings for semi-finish and finish millings are formulated as two constrained optimization problems in a unified framework. Based on the second order Taylor approximation of the distance function, a sequential approximation algorithm along with a hierarchical algorithmic structure is developed for the optimization. Numerical examples are presented to confirm the validity of the proposed approach.展开更多
文摘On June 282022 Sweden and Finland were invited to join NATO.During a symbolic ceremony at the NATO Madrid Summit,all the central actors gathered in front of the cameras,as if to replicate a“family photo”in mini format:representatives from Sweden,Finland,and Turkey,as well as Secretary General Stoltenberg.The atmosphere was mixed:tense because of the dramatic backdrop of the Summit,relief because of the speedy process that had taken the process further.It was finally happening:Sweden and Finland would join the Atlantic Pact.But how did they get there?Over a few hectic months,two countries strongly associated with neutrality had moved their policies from emphasizing continuity to altering the very foundations of their security.Not only NATO critics were taken by surprise.Many NATO supporters were astonished by the rapid pace and the turnaround of the Swedish Social Democratic party.The relatively quick turnaround in Stockholm also meant that questions about the future and the consequences of NATO membership were postponed,not least because the Swedish election campaign put a lid on debate about strategic consequences:what journey awaits in the future and what strategic consequences does a future NATO membership have?This article discusses these questions in the belief that the temporal perspectives are connected:Sweden’s historical collaboration with NATO had significance for the country’s partner relationship,and in turn this influenced how Sweden became a member and what challenges the country faces together with its allies on a new northern flank in Europe.
基金supported by the High-Tech R&D Program of China(2006AA10A106)the open funds of the National Key Laboratory of Crop Genetic Improvement and China National Fundamental Fund of Personnel Training (J0730649)
文摘Mutator transposable element (Mu) has been used as an effective tool to clone maize (Zea mays L.) genes. One opaque endosperm mutant (miol6) was identified in a pool of Mu inserted mutants. A modified method, termed the double selected amplification of insertion flanking fragments (DSAIFF), was employed to isolate the Mu flanking fragments (MFFs) of miol6. The target site duplications (TSDs) isolated from the Msp I and Mse I digested MFFs had a same 9-bp sequence and were confirmed to be the flanking sequence of one identically inserted gene. Co-segregation analysis suggested that the MFFs were associated with the mutant opaque endosperm, and miol6 was mapped in silico onto the physical position ranged from 229 965 021 to 229 965 409 bp of the maize chromosome 4.09 bin. The full-length cDNA of the wild-type gene was obtained by an RT-PCR primer-scanning technique, and Mio16 was found to putatively encode a homolog of the Arabidopsis MAP3K delta-1 protein kinase. RT-PCR result the mRNA expression of miol6 region anchored by primers Mu20 and af276 was not interrupted by Mu insertion. Further researches will be done to elucidate how the expression of miol6 is alternated by Mu insertion.
文摘Transposable elements have been utilized as mutagens to create mutant libraries for functional genomics. Isolation of genomic segments flanking the insertion Mutator (Mu) is a key step in insertion mutagenesis studies. Herein, we adopted a modified AFLP method to identify and isolate Mu-flanking fragments from maize. The method consists of the following steps: 1) double-digestion of genomic DNA with Bgl II/Msp I and ligation of digested fragments to the Bgl II- and Msp I-adaptors; 2) enrichment of a subset of Bgl II/Msp I fragments followed by selective amplification of the Mu-flanking fragments; 3) simultaneous display of AFLP bands derived from the flanking regions for both insert and native Mu transposons; 4) identification and isolation of AFLP bands resulting from Mu insertions by comparing the banding profiles between Mu-induced mutants and their parental lines; and 5) confirmation of flanking fragments related to these Mu insertions. Using this approach, we have isolated flanking fragment(s) resulting from Mu insertion for every Mu-induced mutant, and one such fragment, M196-FF, is found to contain a partial sequence of the DNA topoisomerase I gene Topl. Moreover, the modified AFLP method including all restriction enzymes, adaptors and primers has been optimized in this study. The modified AFLP method has been proved to be simple and efficient in the isolation of Mu-flanking fragments and will find its usefulness in the functional genomics of maize.
文摘This study focuses on the trace and rare earth elements(REE)geochemistry of the Nkporo and Ekenkpon Shales of the Calabar Flank.The main aim is to infer their depositional environment and the degree of their metal enrichment.The shale samples were analyzed using inductively coupled plasma mass spectrometry.The results indicated that the mean concentrations of K,Na,and Fe in Nkporo and Ekenkpon Shales are 1.45,0.4,and 4.17 wt%,and 1.11,0.44,and 5.42 wt%;respectively.The Nkporo Shale is enriched with the following trace elements;P>Mn>Sr>Ba>Zn>Ce>Rb>Zr>V>Cr>Ni and depleted in the following trace elements;Ta>Ge>Sb>Bi>Cd>Ag>Te>In>Hg.While the Ekenkpon Shale is enriched with the following trace elements;P>Mn>Ba>Sr>V>Ce>Zr>Rb>Cr>Zn>Ni and depleted in;Sb>Ge>Bi>Ag>Ce>Te>In>Hg.The concentration of redox-sensitive elements such as V,Ni,Mo,U,Cu,Cr,Re,Cd,Sb,Ti,Mn,and their ratio V/Mo and U/Mo in the black and grey shale samples show different patterns.The REE obtained from the Nkporo and Ekenkpon Shales were PAAS normalized.The Nkporo Shale showed a slightly flat light rare-earth element(LREE),middle rare-earth element(MREE),and heavy rare earth element(HREE)pattern enrichment.Ce/Ce*ranges from 0.95 to 1.09 in Nkporo Shale and 0.67 to 1.40 in Ekenkpon Shale.The Ekenkpon Shale showed a slight LREE,MREE enrichment,and depleted HREE patterns.The Mn contents and U/Mo ratio in Nkporo and Ekenkpon Shales suggests a poor oxygen transitional environment.The V/Mo and V/(V+Ni)ratios indicated that the Nkporo shales were deposited in an anoxic to suboxic conditions and Ekenkpon shales were also deposited under an anoxic to suboxic conditions.The V/Ni ratio indicated that the organic matter in the Nkporo shale is terrigenous while that of the Ekenkpon shales are both terrigenous and marine in origin.
文摘Presents the division of non developable ruled surface into divided small areas and flank milling in these divided areas to improve machining efficiency and machined surface quality by controlling the machining error for each area, and the algorithms developed for generation of tool path and calculation of errors, and concludes from computer simulation results that the algorithms are correct.
基金supported by National Natural Science Foundation of China (No.31172098)the China Postdoctoral Science Foundation (No.2014 M550026)
文摘Background: Soft song is a low-amplitude song produced by many birds. Recent studies have confirmed that soft song is an aggressive signal. For example, the Brownish-flanked Bush Warblers Cettia fortipes use soft song in male-male conflicts, particularly prior to attacks. Although stable signaling systems require that signals be honest on average,models predict that cheating is an acceptable strategy for some individuals or in some contexts.Methods: This study aimed to test the reliability of soft song as an aggressive signal in the brownish-flanked bush warbler. We used mounted specimens accompanied by broadcast songs or soft songs to simulate a male attempting to invade an existing territory.Results: We found the mounted specimen that coupled playback of soft songs suffered more and quicker attacks from the territory owner and that the relationship between soft song and subsequent attack in the territory owner was far from perfect. We observed territory owners that both over-signaled(i.e., produced soft song but did not attack) and under-signaled(i.e., attacked without producing soft song). Under-signaling territory owners were relatively more commonly than were over-signaling territory owners, particularly in simulated intrusion that coupled playback of soft song with a mount specimen.Conclusions: We discuss the cost of producing soft song and the potential benefit of the unreliable use of soft song and propose a new hypothesis for under-signaling with soft song; i.e., under-signaling territory owners might benefit from taking the initiative in fights.
文摘A volcano can be defined as a complex system, not least for the hidden clues related to its internal nature. Innovative models grounded in the Artificial Sciences, have been proposed for a novel pattern recognition analysis at Mt. Etna volcano. The reference monitoring dataset dealt with real data of 28 parameters collected between January 2001 and April 2005, during which the volcano underwent the July-August 2001, October 2002-January 2003 and September 2004-April 2005 flank eruptions. There were 301 eruptive days out of an overall number of 1581 investigated days. The analysis involved successive steps. First, the TWIST algorithm was used to select the most predictive attributes associated with the flank eruption target. During his work, the algorithm TWIST selected 11 characteristics of the input vector: among them SO<sub>2</sub> and CO<sub>2</sub> emissions, and also many other attributes whose linear correlation with the target was very low. A 5 × 2 Cross Validation protocol estimated the sensitivity and specificity of pattern recognition algorithms. Finally, different classification algorithms have been compared to understand if this pattern recognition task may have suitable results and which algorithm performs best. Best results (higher than 97% accuracy) have been obtained after performing advanced Artificial Neural Networks, with a sensitivity and specificity estimates over 97% and 98%, respectively. The present analysis highlights that a suitable monitoring dataset inferred hidden information about volcanic phenomena, whose highly non-linear processes are enhanced.
文摘Objective: To analyze the genomic structure of SNC6, a progesterone-receptor associated protein gene and its regulatory elements in its 5'-flanking region. Methods: Genomic sequence from C, enllank database ( accession number: Z98048) covering the whole SNC6 gene was used to analyze the genonfic stnmture of SNC6 and design primers for PCR amplification of its 5'-flanking region. A 1894 bp fragrnem of the 5’-flanking region ( - 1814 to + 75) was cloned by PCR using genomic DNA from a healthy donor peripheral blood lymphocyte as template. This fragment, as well as 3 shorter derivative fragments ( 1423 bp, 632 bp and 416bp, which correspond to - 1344 to + 75, - 552 to + 75 and - 337 to + 75 respectively), were subeloned into pGL2 series luciferase reporter vectors. These constructs were introduced into colorectal cancer cell line SW620 for transient expression of reporter gene and luefferase activities were measured. Results: The genomic structure analysis showed there are 12 exons for SNC6 gene, which spans 32017 bp (nt71529 to nt39513 in Z98048 sequence). All tmnsfected SW620 cells with the above 5-flanking region-containing constructs showed lueiferase activities. The highest lueiferase activities were measured in transfected cells with vectors containing 1894 bp fragments, and the lowest luefferase activities were measured in tmnsfected cells with vectors containing 416 bp fragments, lmeiferase activities were higher in transfected cells with vectors containing 632 bp fragments than that in tmnsfected cells with vectors containing 1423 bp fragments. Conclusion: The basle tran-scription-promoting element (promoter) for SNC6 expression resides between 0 to - 337, and two transcription-enhancing dements (enhancer) resides between - 337 to - 552 and - 1344 to - 1814, whereas one transcription-inhibiting element (silencer) exists between -552 to - 1344.
基金supported by grants from the National Basic Research Program of China (973 Program,no.2010CB833900-2)the Chinese NSF (no.30870297)
文摘Behavioral studies have shown that flank glands are involved in chemical communication in golden hamstersMesocricetus auratus but little chemical analysis has been conducted on volatiles arising from these glands. Using gas chromatography-mass spectrometry, we detected compounds from the flank glands of males, only eight of which were also produced infemales. Based on these chemical data we performed a number of further experiments. By manipulating light we found that malesexposed to short-photoperiods developed smaller flank glands than those exposed to long-photoperiods. Six flank gland volatilesreduced in relative abundance, which possibly coded for reproductive status of males of this seasonally breeding hamster species.Through dyadic encounters, we were able to induce the formation of dominant-subordinate relationships and show that two glandularcompounds became high in relative abundance and may function as dominance pheromones. Castration eliminated allmale-specific compounds resulting from flank glands, but bilateral ovariectomies only affected one compound in females. Oncethese ovariectomized females were treated with testosterone, their glandular compounds resembled those of males, suggestingthese compounds are under the main control of androgen. Two female putative pheromones, tetradecanoic acid and hexadecanoicacid, were used in binary choice tests and were both found to attract males over females. Applying a solution of these pheromonecompounds to adult males also suppressed their agonistic
文摘Three T-DNA insertional embryonic lethal mutants from NASC (The Nottingham Arabidopsis Stock Center)were first checked with their segregation ratio of abortive and normal seeds and the copy number of T-DNA insertion. The N4081 mutant has a segregation ratio of 1:3.04in average and one T-DNA insertion site according to our assay It was therefore chosen for further analysis. To isolate the joint fragment of T-DNA and plan DNA, the plasmid rescue technique waJs used. pEL-7, one of plasmids from left border of T-DNA, which contained pBR322 was selected from ampicillin plate. The T-DNA fragment of pEL-7 was checked by restriction enzyme analysis and Southern Blot. Restriction analysis confirmed the presence of known sites of EcoRI, PstI and PvuII on it.For confirming the presence of flanking plant DNA in this plasmid, pEL-7 DNA was labeled and hybridized with wild type and mutant plant DNA. The Southern Blot indicated the hybridization band in both of them. Furthermore, the junction of T-DNA/plant DNA was subcloned into bluescript SK+ and sequenced by Applied Biosystem 373A Sequencer. The results showed the 822 bp fragment contained a 274 bp sequence, which is 99.6%homolog (273bp/274 bp) to Ti plasmid pTi 15955 DNA.Ten bp of left 25 bp border repeat were also found in the juction of T-DNA and Plant DNA.Taken together, pEL-7 should contain a joint fragment of T-DNA and flanking plant DNA. This plasmid DNA could be used for the isolation of plant gene, which will be helpful to elucidate the relationship between gene function and plant embryo development.
基金Project(50435020) supported by the National Natural Science Foundation of China
文摘The tool flank begins to wear out as soon as cutting process proceeds. Cutting parameters such as cutting forces and cutting temperature will vary with increasing degree of flank wear. In order to reveal the relationship between them, the theoretical situations of cutting process were analyzed considering the tool flank wear effect. The variation rules of cutting force, residual stress and temperature distributions along with the tool flank wear were analyzed comparing with the sharp tool tip. Through FEM simulation method, affections of the tool flank wear value VB on cutting forces, residual stress and temperature distributions were analyzed. A special result in this simulation is that the thrust force is more sensitive to tool flank wear, which can be used as a recognition method of tool condition monitoring. The FEM simulation analysis result agrees well with the experimental measuring data in public literatures and some experiments made also by the authors.
基金supported by the National Natural Science Foundation of China(Nos.U22A20202 and 52205516)the China Postdoctoral Science Foundation(No.2022 M720641)。
文摘Ruled surfaces found in engineering parts are often blended with a constraint surface,like the blade surface and hub surface of a centrifugal impeller.It is significant to accurately machine these ruled surfaces in flank milling with interference-free and fairing tool path,while current models in fulfilling these goals are complex and rare.In this paper,a tool path planning method with optimal cutter locations(CLs)is proposed for 5-axis flank milling of ruled surfaces under multiple geometric constraints.To be specific,a concise three-point contact tool positioning model is firstly developed for a cylindrical cutter.Different tool orientations arise when varying the three contact positions and a tool orientation pool with acceptable cutter-surface deviation is constructed using a meta-heuristic algorithm.Fairing angular curves are derived from candidates in this pool,and then curve registration for cutter tip point on each determined tool axis is performed in respect of interference avoidance and geometric smoothness.On this basis,an adaptive interval determination model is developed for deviation control of interpolated cutter locations.This model is designed to be independent of the CL optimization process so that multiple CLs can be planned simultaneously with parallel computing technique.Finally,tests are performed on representative surfaces and the results show the method has advantages over previous meta-heuristic tool path planning approaches in both machining accuracy and computation time,and receives the best comprehensive performance compared to other multi-constrained methods when machining an impeller.
文摘Magnesium alloys have many advantages as lightweight materials for engineering applications,especially in the fields of automotive and aerospace.They undergo extensive cutting or machining while making products out of them.Dry cutting,a sustainable machining method,causes more friction and adhesion at the tool-chip interface.One of the promising solutions to this problem is cutting tool surface texturing,which can reduce tool wear and friction in dry cutting and improve machining performance.This paper aims to investigate the impact of dimple textures(made on the flank face of cutting inserts)on tool wear and chip morphology in the dry machining of AZ31B magnesium alloy.The results show that the cutting speed was the most significant factor affecting tool flank wear,followed by feed rate and cutting depth.The tool wear mechanism was examined using scanning electron microscope(SEM)images and energy dispersive X-ray spectroscopy(EDS)analysis reports,which showed that at low cutting speed,the main wear mechanism was abrasion,while at high speed,it was adhesion.The chips are discontinuous at low cutting speeds,while continuous at high cutting speeds.The dimple textured flank face cutting tools facilitate the dry machining of AZ31B magnesium alloy and contribute to ecological benefits.
基金Supported by the National Natural Science Foundation of China (Grant Nos. 50775147 and 50835004)the National Basic Research Program of China ("973" Project) (Grant No. 2005CB724103)the Science & Technology Commission of Shanghai Municipality (Grant No. 07JC14028)
文摘In this paper, optimum positioning of cylindrical cutter for five-axis flank milling of non-developable ruled surface is addressed from the perspective of surface approximation. Based on the developed interchangeability principle, global optimization of the five-axis tool path is modeled as approximation of the tool envelope surface to the data points on the design surface following the minimum zone criterion recommended by ANSI and ISO standards for tolerance evaluation. By using the signed point-to-surface distance function, tool path plannings for semi-finish and finish millings are formulated as two constrained optimization problems in a unified framework. Based on the second order Taylor approximation of the distance function, a sequential approximation algorithm along with a hierarchical algorithmic structure is developed for the optimization. Numerical examples are presented to confirm the validity of the proposed approach.