Objective and Impact Statement:We describe an electroenzymatic mediator(EM)sensor based on an electroenzymatic assembly peak separation strategy,which can efficiently realize the simultaneous detection of 3 typical ca...Objective and Impact Statement:We describe an electroenzymatic mediator(EM)sensor based on an electroenzymatic assembly peak separation strategy,which can efficiently realize the simultaneous detection of 3 typical cardiovascular disease(CVD)metabolites in 5μl of plasma under one test.This work has substantial implications toward improving the efficiency of chronic CVD assessment.Introduction:Monitoring CVD of metabolites is strongly associated with disease risk.Independent and time-consuming detection in hospitals is unfavorable for chronic CVD management.Methods:The EM was flexibly designed by the cross-linking of electron mediators and enzymes,and 3 EM layers with different characteristics were assembled on one electrode.Electrons were transferred under tunable potential;3 metabolites were quantitatively detected by 3 peak currents that correlated with metabolite concentrations.Results:In this study,the EM sensor showed high sensitivity for the simultaneous detection of 3 metabolites with a lower limit of 0.01 mM.The linear correlation between the sensor and clinical was greater than 0.980 for 242 patients,and the consistency of risk assessment was 94.6%.Conclusion:Metabolites could be expanded by the EM,and the sensor could be a promising candidate as a home healthcare tool for CVD risk assessment.展开更多
The Cheria region in Northeastern Algeria has been facing aquifer overexploitation by the agricultural sector and prolonged droughts,resulting in a considerable decline in groundwater levels.This study investigates th...The Cheria region in Northeastern Algeria has been facing aquifer overexploitation by the agricultural sector and prolonged droughts,resulting in a considerable decline in groundwater levels.This study investigates the feasibility of implementing artificial recharge techniques to replenish the Eocene aquifer which serves as the primary water source in the Cheria region.A 3D transient numerical model,based on the finite difference method,was used to simulate groundwater flow from 2021 to 2031 using Visual MODFLOW Flex.During the modelling process,three scenarios were considered:(1)including pumping without a recharge,(2)recharge of the entire area through efficient infiltration without pumping,and(3)artificial recharge using river water infiltration basins at two sites,Draa Douamis sinkholes and Eocene limestone outcrops.The simulation results showed that aquifer exploitation without recharge caused significant drawdowns,which were 3 m to 7 m in the north-eastern part and 8 m to 12 m in the central and southern parts.In contrast,the second scenario,involving recharge without pumping,showed a rise in groundwater levels of 2 m to 2.7 m in the north-eastern part and 3 m to 3.62 m in the central and southern parts.The third scenario,employing artificial recharge,indicated a positive response to artificial recharge,with increased piezometric levels at the proposed sites,signifying a beneficial impact on the aquifer.These findings underline the potential of artificial recharge as a promising approach to address the groundwater depletion and environmental issues in the Cheria Basin.展开更多
基金This work was supported by the National Key R&D Program of China(2021YFB3201202)the National Natural Science Foundation of China(nos.22005331,52275581,and 82372142)+3 种基金the Key Research and Development Program of Jiang su Province(nos.BE2022739,BE2020768,and SBE2022780090)the Youth Innovation Promotion Association of CAS(nos.2019322 and Y2022088)the Instrument Developing Project of the Chinese Academy of Sciences(nos.YJKYYQ20200046 and ZDKYYQ20210004)the Science and Technology Development Program of Suzhou(no.SJC2021019).
文摘Objective and Impact Statement:We describe an electroenzymatic mediator(EM)sensor based on an electroenzymatic assembly peak separation strategy,which can efficiently realize the simultaneous detection of 3 typical cardiovascular disease(CVD)metabolites in 5μl of plasma under one test.This work has substantial implications toward improving the efficiency of chronic CVD assessment.Introduction:Monitoring CVD of metabolites is strongly associated with disease risk.Independent and time-consuming detection in hospitals is unfavorable for chronic CVD management.Methods:The EM was flexibly designed by the cross-linking of electron mediators and enzymes,and 3 EM layers with different characteristics were assembled on one electrode.Electrons were transferred under tunable potential;3 metabolites were quantitatively detected by 3 peak currents that correlated with metabolite concentrations.Results:In this study,the EM sensor showed high sensitivity for the simultaneous detection of 3 metabolites with a lower limit of 0.01 mM.The linear correlation between the sensor and clinical was greater than 0.980 for 242 patients,and the consistency of risk assessment was 94.6%.Conclusion:Metabolites could be expanded by the EM,and the sensor could be a promising candidate as a home healthcare tool for CVD risk assessment.
文摘The Cheria region in Northeastern Algeria has been facing aquifer overexploitation by the agricultural sector and prolonged droughts,resulting in a considerable decline in groundwater levels.This study investigates the feasibility of implementing artificial recharge techniques to replenish the Eocene aquifer which serves as the primary water source in the Cheria region.A 3D transient numerical model,based on the finite difference method,was used to simulate groundwater flow from 2021 to 2031 using Visual MODFLOW Flex.During the modelling process,three scenarios were considered:(1)including pumping without a recharge,(2)recharge of the entire area through efficient infiltration without pumping,and(3)artificial recharge using river water infiltration basins at two sites,Draa Douamis sinkholes and Eocene limestone outcrops.The simulation results showed that aquifer exploitation without recharge caused significant drawdowns,which were 3 m to 7 m in the north-eastern part and 8 m to 12 m in the central and southern parts.In contrast,the second scenario,involving recharge without pumping,showed a rise in groundwater levels of 2 m to 2.7 m in the north-eastern part and 3 m to 3.62 m in the central and southern parts.The third scenario,employing artificial recharge,indicated a positive response to artificial recharge,with increased piezometric levels at the proposed sites,signifying a beneficial impact on the aquifer.These findings underline the potential of artificial recharge as a promising approach to address the groundwater depletion and environmental issues in the Cheria Basin.
