目的探讨口腔鳞状细胞癌(OSCC)中细胞型FLICE样抑制蛋白(c FLIP)及T-box转录因子(TBX2)的表达及其临床意义。方法采用免疫组化检测OSCC 57例、口腔黏膜上皮异常增生组织(DOM)38例及正常黏膜组织(NOM)30例中c FLIP、TBX2蛋白表达情况。...目的探讨口腔鳞状细胞癌(OSCC)中细胞型FLICE样抑制蛋白(c FLIP)及T-box转录因子(TBX2)的表达及其临床意义。方法采用免疫组化检测OSCC 57例、口腔黏膜上皮异常增生组织(DOM)38例及正常黏膜组织(NOM)30例中c FLIP、TBX2蛋白表达情况。运用逆转录多聚酶链反应(RT-PCR)技术检测OSCC、DOM和NOM中的c FLIP m RNA和TBX2 m RNA表达水平。结果在OSCC、DOM及NOM中,c FLIP的阳性表达率分别为91.23%、84.21%和10.00%;TBX2的阳性表达率分别为87.72%、81.85%和26.67%。c FLIP及TBX2的表达水平与患者的年龄、性别无关,差异无统计学意义(P>0.05),而肿瘤TNM分期以及有无淋巴结转移间差异有统计学意义(P<0.05)。OSCC中c FLIP m RNA及TBX2 m RNA的表达水平明显高于DOM及NOM。c FLIP m RNA及TBX2m RNA的表达水平与患者的年龄、性别无关,差异无统计学意义(P>0.05),而肿瘤TNM分期以及有无淋巴结转移间差异有统计学意义(P<0.05)。Spearman相关性分析显示c FLIP及TBX2的表达呈正相关(r=0.768,P<0.05)。结论 c FLIP及TBX2在OSCC中呈高表达,两者可能在OSCC的发生、发展中发挥重要的作用。展开更多
AIM: To investigate the expression of cellular FLICE (Fas associated death domain-like IL-lbeta-converting enzyme)-inhibitory protein (c-FLIP) and its association with p53 mutation in colon cancer. METHODS: Immunohist...AIM: To investigate the expression of cellular FLICE (Fas associated death domain-like IL-lbeta-converting enzyme)-inhibitory protein (c-FLIP) and its association with p53 mutation in colon cancer. METHODS: Immunohistochemical staining of c-FLIP and mutant p53 by using specific antibodies was performed by the standard streptavidin-peroxidase technique for 45 colon cancer tissue samples with matched normal tissues. Semi-quantitative reverse transcriptional (RT)-PCR was used to measure c-FLIP mRNA levels, t-test statistical method was used in data analyses. RESULTS: c-FLIP mRNA was expressed in all colon cancer tissues and its level (0.63±0.12) was significantly higher than that in normal tissues (0.38±0.10, P<0.01). Immuno-histochemically, c-FLIP protein was also expressed in all colon cancers (45/45) and 71.1% (32/45) showed an intense immunostaining, in contrast, 93.3% (42/45) of normal colonic mucosa showed positive staining and none of them immunostained intensely. The quantity of c-FLIP protein was significantly higher in cancer tissues than in normal mucosa (7.04±1.20 vs 5.21±0.86, P<0.01). Positive staining of mutant p53 protein was found in 60% (27/45) colon cancers. c-FLIP mRNA level was decreased in p53 positive group compared with p53 negative cancer tissues (0.59±0.13 vs0.69±0.14, P<0.01), but c-FLIP protein had a significantly higher level in p53 positive cancer tissues than in negative ones (7.57±1.30 vs6.25±1.27, P<0.01). CONCLUSION: c-FLIP is specially overexpressed in colon cancers and it might contribute to carcinogenesis of normal colonic mucosa. p53 may exert transcriptional upregulation effects on c-FLI P gene and more potent effects on promoting the degradation of c-FLIP protein.展开更多
文摘目的探讨口腔鳞状细胞癌(OSCC)中细胞型FLICE样抑制蛋白(c FLIP)及T-box转录因子(TBX2)的表达及其临床意义。方法采用免疫组化检测OSCC 57例、口腔黏膜上皮异常增生组织(DOM)38例及正常黏膜组织(NOM)30例中c FLIP、TBX2蛋白表达情况。运用逆转录多聚酶链反应(RT-PCR)技术检测OSCC、DOM和NOM中的c FLIP m RNA和TBX2 m RNA表达水平。结果在OSCC、DOM及NOM中,c FLIP的阳性表达率分别为91.23%、84.21%和10.00%;TBX2的阳性表达率分别为87.72%、81.85%和26.67%。c FLIP及TBX2的表达水平与患者的年龄、性别无关,差异无统计学意义(P>0.05),而肿瘤TNM分期以及有无淋巴结转移间差异有统计学意义(P<0.05)。OSCC中c FLIP m RNA及TBX2 m RNA的表达水平明显高于DOM及NOM。c FLIP m RNA及TBX2m RNA的表达水平与患者的年龄、性别无关,差异无统计学意义(P>0.05),而肿瘤TNM分期以及有无淋巴结转移间差异有统计学意义(P<0.05)。Spearman相关性分析显示c FLIP及TBX2的表达呈正相关(r=0.768,P<0.05)。结论 c FLIP及TBX2在OSCC中呈高表达,两者可能在OSCC的发生、发展中发挥重要的作用。
基金Supported by the Scientific Research Foundation for Returned Overseas Chinese ScholarsState Education Ministry, China (2003)14
文摘AIM: To investigate the expression of cellular FLICE (Fas associated death domain-like IL-lbeta-converting enzyme)-inhibitory protein (c-FLIP) and its association with p53 mutation in colon cancer. METHODS: Immunohistochemical staining of c-FLIP and mutant p53 by using specific antibodies was performed by the standard streptavidin-peroxidase technique for 45 colon cancer tissue samples with matched normal tissues. Semi-quantitative reverse transcriptional (RT)-PCR was used to measure c-FLIP mRNA levels, t-test statistical method was used in data analyses. RESULTS: c-FLIP mRNA was expressed in all colon cancer tissues and its level (0.63±0.12) was significantly higher than that in normal tissues (0.38±0.10, P<0.01). Immuno-histochemically, c-FLIP protein was also expressed in all colon cancers (45/45) and 71.1% (32/45) showed an intense immunostaining, in contrast, 93.3% (42/45) of normal colonic mucosa showed positive staining and none of them immunostained intensely. The quantity of c-FLIP protein was significantly higher in cancer tissues than in normal mucosa (7.04±1.20 vs 5.21±0.86, P<0.01). Positive staining of mutant p53 protein was found in 60% (27/45) colon cancers. c-FLIP mRNA level was decreased in p53 positive group compared with p53 negative cancer tissues (0.59±0.13 vs0.69±0.14, P<0.01), but c-FLIP protein had a significantly higher level in p53 positive cancer tissues than in negative ones (7.57±1.30 vs6.25±1.27, P<0.01). CONCLUSION: c-FLIP is specially overexpressed in colon cancers and it might contribute to carcinogenesis of normal colonic mucosa. p53 may exert transcriptional upregulation effects on c-FLI P gene and more potent effects on promoting the degradation of c-FLIP protein.
