The binding of flucytosine to bovine serum albumin (BSA) was studied by means of fluorescence and absorption spectra under the conditions of simulant clay physiology. It showed a powerful ability to quench the fluor...The binding of flucytosine to bovine serum albumin (BSA) was studied by means of fluorescence and absorption spectra under the conditions of simulant clay physiology. It showed a powerful ability to quench the fluorescence launching from BSA. After analyzing the fluorescence quenching data by Stem-Volmer equation and Lineweaver-Burk double-reciprocal equation, it was found that they matched the latter better and so they belonged to static quenching. The binding constant was calculated to be 5.710 × 10^3 L·mol^-1 at 297 K. The binding locality was a distance 2.49 nm away from tryptophan residue-212 based on Foster's non-radiation energy transfer mechanism. The binding power is mainly the hydrogen bond and van der Waals force according to the thermodynamic parameters. The information of BSA conformation was acquired by synchronous fluorescence spectrum and three-dimensional fluorescence spectrum.展开更多
Background Amphotericin B (0.7 mg/kg) with flucytosine is the standard treatment for cryptococcal meningitis.However,the long treatment course can induce adverse reactions in patients; therefore,reducing the dose ma...Background Amphotericin B (0.7 mg/kg) with flucytosine is the standard treatment for cryptococcal meningitis.However,the long treatment course can induce adverse reactions in patients; therefore,reducing the dose may decrease such reactions.We performed a retrospective analysis of treatment effects and adverse reactions when amphotericin B (0.4 mg/kg or 0.7 mg/kg per day) and flucytosine were used together to treat HIV-negative patients with cryptococcal meningitis.Methods Retrospective analysis was conducted on inpatients at the First Affiliated Hospital,College of Medicine,Zhejiang University (January 2005 to December 2009).Low- or high-dose amphotericin B (0.4 or 0.7 mg/kg per day,respectively) plus flucytosine was used.The negative conversion rate of Cryptococcus in the cerebrospinal fluid (CSF),patient mortality,and the incidence of side effects for the two groups (low- vs.high-dose) were compared immediately after treatment and 2 and 10 weeks later.Data were analyzed by the Student's t test,chi-square tests using SPSS 12.0 statistical soitware.Results Two weeks post-treatment,Cryptococcus negative CSF rates were 78% (18/23) in the low-dose group and 87% (13/15) in the high-dose group (P=0.28).Ten weeks post-treatment,both groups were negative.The mortality rate was 8% (2/25) in the low-dose group and 17% (3/18) in the high-dose group (P=-0.25).There was a statistically significant difference in the incidence of adverse events between the groups,48% (12/25) and 78% (14/18) in the low- and high-dose groups,respectively (P=0.04).Adverse events that required a change in treatment program in the low-dose group were 12% (3/25) compared to 39% (7/18) in the high-dose group (P=-0.04).Conclusion Low-dose treatment regimens were better tolerated展开更多
基金Project supported by the National Natural Science Foundation of China (No. 20373051) and the National Natural Science Foundation of Hubei Province in China (No. 2005ABA067).
文摘The binding of flucytosine to bovine serum albumin (BSA) was studied by means of fluorescence and absorption spectra under the conditions of simulant clay physiology. It showed a powerful ability to quench the fluorescence launching from BSA. After analyzing the fluorescence quenching data by Stem-Volmer equation and Lineweaver-Burk double-reciprocal equation, it was found that they matched the latter better and so they belonged to static quenching. The binding constant was calculated to be 5.710 × 10^3 L·mol^-1 at 297 K. The binding locality was a distance 2.49 nm away from tryptophan residue-212 based on Foster's non-radiation energy transfer mechanism. The binding power is mainly the hydrogen bond and van der Waals force according to the thermodynamic parameters. The information of BSA conformation was acquired by synchronous fluorescence spectrum and three-dimensional fluorescence spectrum.
文摘Background Amphotericin B (0.7 mg/kg) with flucytosine is the standard treatment for cryptococcal meningitis.However,the long treatment course can induce adverse reactions in patients; therefore,reducing the dose may decrease such reactions.We performed a retrospective analysis of treatment effects and adverse reactions when amphotericin B (0.4 mg/kg or 0.7 mg/kg per day) and flucytosine were used together to treat HIV-negative patients with cryptococcal meningitis.Methods Retrospective analysis was conducted on inpatients at the First Affiliated Hospital,College of Medicine,Zhejiang University (January 2005 to December 2009).Low- or high-dose amphotericin B (0.4 or 0.7 mg/kg per day,respectively) plus flucytosine was used.The negative conversion rate of Cryptococcus in the cerebrospinal fluid (CSF),patient mortality,and the incidence of side effects for the two groups (low- vs.high-dose) were compared immediately after treatment and 2 and 10 weeks later.Data were analyzed by the Student's t test,chi-square tests using SPSS 12.0 statistical soitware.Results Two weeks post-treatment,Cryptococcus negative CSF rates were 78% (18/23) in the low-dose group and 87% (13/15) in the high-dose group (P=0.28).Ten weeks post-treatment,both groups were negative.The mortality rate was 8% (2/25) in the low-dose group and 17% (3/18) in the high-dose group (P=-0.25).There was a statistically significant difference in the incidence of adverse events between the groups,48% (12/25) and 78% (14/18) in the low- and high-dose groups,respectively (P=0.04).Adverse events that required a change in treatment program in the low-dose group were 12% (3/25) compared to 39% (7/18) in the high-dose group (P=-0.04).Conclusion Low-dose treatment regimens were better tolerated