FM4-64,a kind of membrane-selective fluorescent dye,was utilized to stain the membrane structure of Botrytis cinerea and Phytophthora capsici.The effect of FM4-64 on hypha was observed under confocal microscope.The re...FM4-64,a kind of membrane-selective fluorescent dye,was utilized to stain the membrane structure of Botrytis cinerea and Phytophthora capsici.The effect of FM4-64 on hypha was observed under confocal microscope.The results indicated that the plasma membrane,speta and vesicles of the hyphae could be stained with FM4-64 at the concentrations of 1 to 20 μmol/L.The optimal dye concentration was 6 μmol/L for the both pathogens.At the same concentration of FM4-64,the staining of organelles was in a time-dependent manner and the different organelles were stained in diferrent times.The staining of plasma membrane and vesicles should be completed in 5 minutes,as other organelles can be stained after 10 minutes.展开更多
Multiphoton microscopy(MPM)is a powerful imaging technology for brain research.The imaging depth in MPM is partly determined by emission wavelength of fluorescent labels.It has been demonstrated that a longer emission...Multiphoton microscopy(MPM)is a powerful imaging technology for brain research.The imaging depth in MPM is partly determined by emission wavelength of fluorescent labels.It has been demonstrated that a longer emission wavelength is favorable for signal detection as imaging depth increases.However,there has been no comparison with near-infrared(NIR)emission.In order to quantitatively analyze the effect of emission wavelength on 3-photon imaging of mouse brains in vivo,we utilize the same excitation wavelength to excite a single fluorescent dye and simultaneously collect NIR and orange-red emission fluorescence at 828 nm and 620 nm,respectively.Both experimental and simulation results show that as the imaging depth increases,NIR emission decays less than orange-red fluorescent emission.These results show that it is preferable to shift the emission wavelength to NIR to enable more e±cient signal collection deep in the brain.展开更多
文章主要介绍一种现场可编程逻辑门阵列(Field Programmable Gate Array,FPGA)控制数字频率合成器(Direct Digital Synthesizer,DDS)实现四进制移频键控(Quaternary Frequency Shift Keying,4FSK)&频率调制(Frequency Modulation,FM...文章主要介绍一种现场可编程逻辑门阵列(Field Programmable Gate Array,FPGA)控制数字频率合成器(Direct Digital Synthesizer,DDS)实现四进制移频键控(Quaternary Frequency Shift Keying,4FSK)&频率调制(Frequency Modulation,FM)调制载波的设计方案,给出技术指标参数、硬件组成框图以及信号处理流程,对4FSK的调制信号和FM信号产生的实施方法进行探讨,并对电路框图中的关键器件进行国产化设计选型。展开更多
文摘FM4-64,a kind of membrane-selective fluorescent dye,was utilized to stain the membrane structure of Botrytis cinerea and Phytophthora capsici.The effect of FM4-64 on hypha was observed under confocal microscope.The results indicated that the plasma membrane,speta and vesicles of the hyphae could be stained with FM4-64 at the concentrations of 1 to 20 μmol/L.The optimal dye concentration was 6 μmol/L for the both pathogens.At the same concentration of FM4-64,the staining of organelles was in a time-dependent manner and the different organelles were stained in diferrent times.The staining of plasma membrane and vesicles should be completed in 5 minutes,as other organelles can be stained after 10 minutes.
基金work is funded by the National Natural Sci-ence Foundation of China(Grant/Award Numbers 62075135 and 61975126)Shenzhen Science and Technology Planning Project(ZDSYS2021-0623092006020)+2 种基金Key R&D Program of Shandong Province(Grant Number 2021CXGC010202)the Science and Technology Innovation Commission of Shenzhen(Grant/Award Numbers JCYJ201908-08174819083 and JCYJ20190808175201640)and Natural Science Foundation of Shandong Province(Grant Number ZR2022MA046)Major Innovation Projects for Integrating Science,Education&Industry of Qilu University of Technology(Shan-dong Academy of Sciences,Grant Number 2022JBZ01-04).
文摘Multiphoton microscopy(MPM)is a powerful imaging technology for brain research.The imaging depth in MPM is partly determined by emission wavelength of fluorescent labels.It has been demonstrated that a longer emission wavelength is favorable for signal detection as imaging depth increases.However,there has been no comparison with near-infrared(NIR)emission.In order to quantitatively analyze the effect of emission wavelength on 3-photon imaging of mouse brains in vivo,we utilize the same excitation wavelength to excite a single fluorescent dye and simultaneously collect NIR and orange-red emission fluorescence at 828 nm and 620 nm,respectively.Both experimental and simulation results show that as the imaging depth increases,NIR emission decays less than orange-red fluorescent emission.These results show that it is preferable to shift the emission wavelength to NIR to enable more e±cient signal collection deep in the brain.
文摘文章主要介绍一种现场可编程逻辑门阵列(Field Programmable Gate Array,FPGA)控制数字频率合成器(Direct Digital Synthesizer,DDS)实现四进制移频键控(Quaternary Frequency Shift Keying,4FSK)&频率调制(Frequency Modulation,FM)调制载波的设计方案,给出技术指标参数、硬件组成框图以及信号处理流程,对4FSK的调制信号和FM信号产生的实施方法进行探讨,并对电路框图中的关键器件进行国产化设计选型。
