贝母辛的结构修正

从川产家种华西贝母(Fritillaria siechuanica)鳞茎中分出贝母辛(peimisine)(碱Ⅲ)。应用光谱法(~1H—~1H,^(13)C—~1H COSY,2D—NOE),对文献报告的贝母辛的结构作了修正,并证明FR—5与贝母辛系同一化合物。指出湖贝辛结构的某些错误以及对松贝辛的结构提出怀疑,认为其可能为贝母辛。

蛹虫草菌丝体中单核苷酸类组分的高效液相色谱分析

目的测定蛹虫草菌丝体中5-′CMP(5′-胞苷酸)、5-′UMP(5′-尿苷酸)、5-′GMP(5′-鸟苷酸)、5-′IMP(5′-嘌呤核苷酸)、5-′XMP(5′-黄嘌呤核苷酸)、5-′AMP(5′-腺苷酸)等六种核苷酸的含量。方法用反相高效液相色谱法,以SH IMAD-ZU C18(150 mm×4.6 mm I.D,5μm)为色谱柱;以缓冲盐(KH2PO4-H3PO4)-甲醇(99.5∶0.5,V/V,pH3.75)体系为流动相,在流速0.8 mL/m in,柱温26°C,检测波长260 nm,进样量20μl的条件下对样品中5′-核苷酸的含量进行了分析测定。结果蛹虫草菌丝体中上述6种核苷酸含量分别为173.0,824.9,92.9,2114.0,79.6,231.4μg/g。结论该法快速简便,重现性好,能有效的测定样品中核苷酸类成分。

天麻蜜环菌粉正己烷提取物的分离和抗神经性炎活性研究

为了探究天麻蜜环菌粉正己烷提取物抗炎作用的物质基础及抗炎机制,本文首先对天麻蜜环菌粉正己烷提取物进行薄层层析和中低压柱层析分离,获得了具有显著抗炎活性的组分5(Fr.5),进一步通过硅胶柱分离,得到主成分较为集中的组分5-1(Fr.5-1),液质联用(LC-MS)及高效液相色谱分析推测Fr.5-1的主成分为大豆黄素;其次,通过脂多糖(LPS)诱导小胶质细胞(BV2)炎症模型考察Fr.5-1不同剂量(10、30、100μg/m L)对炎症因子一氧化氮(NO)和肿瘤坏死因子(TNF-α)表达的影响,发现中高剂量(30、100μg/m L)的Fr.5-1能显著抑制LPS诱导的BV2细胞炎症因子NO及TNF-α的分泌。研究表明天麻蜜环菌粉提取物Fr.5-1具有抗炎活性,其机制可能是通过抑制炎症因子的表达和分泌。

Prospective Assessment of Direction of Cervical Canal in Routine Hysteroscopy

Introduction: If the direction of the cervical canal is known, the commonly performed procedures as sono salpingography, embryo transfer, IUD insertion, cervical dilation etc. will be easier. The reported resultant trauma to the cervical canal and uterus during these procedures also can be avoided. As we know the cervical canal is tortuous in majority of cases, but the exact course is not yet reported or known. Objective: The objective of the present study was to try to identify the various directions of cervical canal while performing routine hysteroscopic surgeries. Methods: Four-point cervical canal direction was assessed while performing routine hysteroscopic procedures using 5fr Bettocchi operative assembly with 2.9 mm 12-degree telescope (Karl-Storz). The study group was patients with infertility who required hysteroscopy and laparoscopic evaluation as per infertility treatment protocol or else required hysteroscopy for AUB. The study was carried out at tertiary care referral hospital for minimal access surgeries for a period was of 2 yr. 9 months year from May 2017 to Feb 2020. Results: Down-right or left-up-straight combination of movement (DRUS, DLUS) was the most common cervical direction found in 72 % patients. If DURS (down-up-right-straight) movement is added these 3 movements together are seen in about 82% of patients. No cervical dilation is required when 5 fr hysteroscopic assembly was used in study group. No operative complications were found. Conclusion: DRUS and DLUS (down-right or left-up and straight) combination of movements are most frequent direction of cervical canal observed in 72% of patients.