The geomagnetic field(GMF)is an environmental cue that provides directional information for animals.The intensity of GMF is varied over space and time.Variations in the GMF intensity afect the navigation of animals an...The geomagnetic field(GMF)is an environmental cue that provides directional information for animals.The intensity of GMF is varied over space and time.Variations in the GMF intensity afect the navigation of animals and their physiology.In this study,the phototaxis of the migratory insect rice planthopper Nilaparvata lugens(N.lugens)and frataxin in N.lugens(NI-fh),which is a mitochondrial protein required for cellular iron homeostasis and iron-sulfur cluster assembly,were investigated by using different intensities of magnetic field.From the results,individuals of N.lugens showed decreased phototaxis when reared and tested in a behavioral arena under a strong magnetic field.Besides the reduction in performance,an accompanying ffect of the strong magnetic field condition was a reduced level of Nl-fh-messenger RNA,and a NI-fh knockdown indeed impaired the phototactic behavior in a tested sample of insects.This leads to the conclusion that the expression of frataxin is dependent on the strength of the surrounding magnetic field and that functional frataxin facilitates phototactic behavior in N.lugens.展开更多
AtNfsl is the Arabidopsis thaliana mitochondrial homolog of the bacterial cysteine desulfurases NifS and IscS, having an essential role in cellular Fe-S cluster assembly. Homology modeling of AtNfslm predicts a high g...AtNfsl is the Arabidopsis thaliana mitochondrial homolog of the bacterial cysteine desulfurases NifS and IscS, having an essential role in cellular Fe-S cluster assembly. Homology modeling of AtNfslm predicts a high global similarity with E. coil IscS showing a full conservation of residues involved in the catalytic site, whereas the chloroplastic AtNfs2 is more similar to the Synechocystis sp. SufS. Pull-down assays showed that the recombinant mature form, AtNfslm, specifically binds to Arabidopsis frataxin （AtFH）. A hysteretic behavior, with a lag phase of several minutes, was observed and hysteretic parameters were affected by pre-incubation with AtFH. Moreover, AtFH modulates AtNfslm kinetics, increasing Vmax and decreasing the S0.5 value for cysteine. Results suggest that AtFH plays an important role in the early steps of Fe-S cluster formation by regulating AtNfsl activity in plant mitochondria.展开更多
基金We thank Wei Pan for generous assistance in the setup and maintenance of magnetic field equipment of solenoidal coils.This research was supported by the National Nat-ural Science Foundation of China(3167085,31870367,31470454)the Natural Science Foundation of Jiangsu Province Youth Fund(BK20160717)the Natural Sci-ence Foundation ofChina for Young Scholars(31701787).
文摘The geomagnetic field(GMF)is an environmental cue that provides directional information for animals.The intensity of GMF is varied over space and time.Variations in the GMF intensity afect the navigation of animals and their physiology.In this study,the phototaxis of the migratory insect rice planthopper Nilaparvata lugens(N.lugens)and frataxin in N.lugens(NI-fh),which is a mitochondrial protein required for cellular iron homeostasis and iron-sulfur cluster assembly,were investigated by using different intensities of magnetic field.From the results,individuals of N.lugens showed decreased phototaxis when reared and tested in a behavioral arena under a strong magnetic field.Besides the reduction in performance,an accompanying ffect of the strong magnetic field condition was a reduced level of Nl-fh-messenger RNA,and a NI-fh knockdown indeed impaired the phototactic behavior in a tested sample of insects.This leads to the conclusion that the expression of frataxin is dependent on the strength of the surrounding magnetic field and that functional frataxin facilitates phototactic behavior in N.lugens.
文摘AtNfsl is the Arabidopsis thaliana mitochondrial homolog of the bacterial cysteine desulfurases NifS and IscS, having an essential role in cellular Fe-S cluster assembly. Homology modeling of AtNfslm predicts a high global similarity with E. coil IscS showing a full conservation of residues involved in the catalytic site, whereas the chloroplastic AtNfs2 is more similar to the Synechocystis sp. SufS. Pull-down assays showed that the recombinant mature form, AtNfslm, specifically binds to Arabidopsis frataxin （AtFH）. A hysteretic behavior, with a lag phase of several minutes, was observed and hysteretic parameters were affected by pre-incubation with AtFH. Moreover, AtFH modulates AtNfslm kinetics, increasing Vmax and decreasing the S0.5 value for cysteine. Results suggest that AtFH plays an important role in the early steps of Fe-S cluster formation by regulating AtNfsl activity in plant mitochondria.
