Objective: To investigate the effects of friedelin(terpenoid) and 8-hydroxyisocapnolactone-2-3-diol(coumarin) with concentration 10 mM, 30 m M, and 100 mM on inhibiting mast cells(MCs) degranulation.Methods: The inves...Objective: To investigate the effects of friedelin(terpenoid) and 8-hydroxyisocapnolactone-2-3-diol(coumarin) with concentration 10 mM, 30 m M, and 100 mM on inhibiting mast cells(MCs) degranulation.Methods: The investigation was performed in vitro by administering each compound into rat peritoneal MCs and rat basophilic leukemia-2 H3 cells followed by activation with50 mg/m L of compound 48/80 or 1 m M of ionomycin. The concentration of histamine released from each group was measured by a high-performance liquid chromatographyfluorometry system with post-column derivatization using o-phthalaldehyde.Results: 8-Hydroxyisocapnolactone-2-3-diol inhibited degranulation of compound 48/80 activated-rat peritoneal MCs with the histamine release percentages of 74.57%,72.21% and 51.79% when the 10 mM, 30 m M and 100 mM concentrations were used,respectively. Where as about 81% histamine was released by the control group.Degranulation inhibition ability was also observed in ionomycin-activated rat basophilic leukemia-2 H3 cells. In contrast, friedelin failed to inhibit degranulation in either cell type.The inhibition of 8-hydroxyisocapnolactone-2-3-diol was not related to the depletion of histamine synthesis as implied by the total histamine measurement.Conclusions: These results exhibit the promising of 8-hydroxyisocapnolactone-2-3-diol is a potential parent structure for developing a MCs stabilizer.展开更多
Six Chemical Constituents were isolated from the underground parts of Globba racemosa Smith (Zingiberaceas)and identitied as friedelin(1),cycloeucalenol(2) 3-hydroxy-stigmast -5-7-one(3), stigmast-5-en-7-one (5) and s...Six Chemical Constituents were isolated from the underground parts of Globba racemosa Smith (Zingiberaceas)and identitied as friedelin(1),cycloeucalenol(2) 3-hydroxy-stigmast -5-7-one(3), stigmast-5-en-7-one (5) and sitosterol(6) based on spectral data. 1-5 wereisolated form the Plants of Zingiberaceae family for the first time and triterpenoids were found in Zingiberaceae for the first time too.展开更多
文摘Objective: To investigate the effects of friedelin(terpenoid) and 8-hydroxyisocapnolactone-2-3-diol(coumarin) with concentration 10 mM, 30 m M, and 100 mM on inhibiting mast cells(MCs) degranulation.Methods: The investigation was performed in vitro by administering each compound into rat peritoneal MCs and rat basophilic leukemia-2 H3 cells followed by activation with50 mg/m L of compound 48/80 or 1 m M of ionomycin. The concentration of histamine released from each group was measured by a high-performance liquid chromatographyfluorometry system with post-column derivatization using o-phthalaldehyde.Results: 8-Hydroxyisocapnolactone-2-3-diol inhibited degranulation of compound 48/80 activated-rat peritoneal MCs with the histamine release percentages of 74.57%,72.21% and 51.79% when the 10 mM, 30 m M and 100 mM concentrations were used,respectively. Where as about 81% histamine was released by the control group.Degranulation inhibition ability was also observed in ionomycin-activated rat basophilic leukemia-2 H3 cells. In contrast, friedelin failed to inhibit degranulation in either cell type.The inhibition of 8-hydroxyisocapnolactone-2-3-diol was not related to the depletion of histamine synthesis as implied by the total histamine measurement.Conclusions: These results exhibit the promising of 8-hydroxyisocapnolactone-2-3-diol is a potential parent structure for developing a MCs stabilizer.
文摘Six Chemical Constituents were isolated from the underground parts of Globba racemosa Smith (Zingiberaceas)and identitied as friedelin(1),cycloeucalenol(2) 3-hydroxy-stigmast -5-7-one(3), stigmast-5-en-7-one (5) and sitosterol(6) based on spectral data. 1-5 wereisolated form the Plants of Zingiberaceae family for the first time and triterpenoids were found in Zingiberaceae for the first time too.
文摘利用硅胶柱色谱反复层析从黄背越橘的乙酸乙酯部分分得7个化合物,根据理化性质和光谱数据鉴定,分别为β-谷甾醇(β-sitosterol,Ⅰ),熊果酸(ursolic acid,Ⅱ),蒲公英萜醇(taraxerol,Ⅲ),蒲公英萜酮(taraxerone,Ⅳ),木栓酮(friedelin,Ⅴ),木栓醇(friedelinol,Ⅵ),19,24-d ihydroxyurs-12-en-3-one-28-oic ac id(Ⅶ).