Induced Polyploidy via Colchicine Treatment Increases Flower Size and Fruit Weight in Cape Gooseberry(Physalis peruviana L.)

An induced polyploid plant through colchicine treatment offers probably the best scope for improvement in flower size and fruit weight.Thus,in the present study,an attempt was made to induce polyploidy in Cape gooseberry using colchicine with the objective of creating more genetic variability.The colchicine concentrations were used as 0.10%(C1),0.20%(C2)and 0.40%(C3)for the duration 12(H1),24(H2)and 36(H3)hours for each concentration with seedling apex dip method(M1),cotton plug method(M2)and lanolin paste method(M3).The plants treated with 0.10%of colchicine by cotton plug method for 12 h showed the better performance during the years 2017-2018 and 2018-2019 in respect of more delay in the flower bud emergence(54 d and 53 d from the date of transplanting),anthesis(19 d and 20 d from the first appearance of bud to full anthesis of flower)and fruit setting(8.00 d and 9.00 d from the date of anthesis),bigger flower size(2.93 cm2 and 3.00 cm2)than the untreated plants.The lower percentage of pollen viability(40.33%and 40.67%)was noticed in the same treatment in comparison to control(70.33%and 72.33%).The fruit maturity was also extended(59 d and 60 d from the date of fruit set)with bigger sized fruits(length:2.53 cm and 2.57 cm,breadth:2.27 cm and 2.33 cm)as well as more fruit weight(8.70 g and 8.33 g)by the application of colchicine at 0.10%with cotton plug method for 12 h.On the basis of results obtained,the application of colchicine at 0.10%for 12 h duration with cotton plug method was found to be the best and effective treatment for induction of polyploidy as well as more flower size and fruit weight in Cape gooseberry.

Identificatio of Quantitative Trait Loci for Fruit Weight, Soluble Solids Content, and Plant Morphology Using an Introgression Line Population of Solanum pennellii in a Fresh Market Tomato Inbred Line

Introgression lines are convenient populations for the identification fine-mapping and functional analysis of genes that are responsible for variations in traits, particularly quantitative trait genes. An introgression line population of Solanum pennellii LA0716 in a fresh market tomato inbred line 1052 was developed by our group. This population was composed of 214 lines. In the present study, fi e quantitative trait loci(QTLs)for fruit weight, two QTLs for soluble solids content(SSC), three QTLs for plant height, and one QTL for leaf size were identifie using this introgression line population. Among these, fw3 a and fw4 a for fruit weight, ssc7 a for SSC, h4t2 a, h4t3 a, and h4t7 a for plant height, and lz12 a for leaf size were determined to be novel loci. These results serve as a foundation for fine-mappin and functional analysis of genes underlying these QTLs.

Genetic Analysis of Weight per Fruit and Fruit Length in Bitter Gourd

[Objectives]The paper was to verify the genetic mechanism of weight per fruit and fruit length in bitter gourd and to provide guidance for formulating breeding strategy.[Methods]In this study,a cross was made between CN19-1 and Thai4-6,and a Fsegregation population was also constructed.The genetic characteristics of weight per fruit and fruit length were analyzed by employing major gene plus polygene mixed genetic model.[Results]The weight per fruit and fruit length showed continuous distribution in Fsegregation population.The optimal model for weight per fruit and fruit length was the same(A-1 model).The major gene additive effect value of weight per fruit was 46.1474,the dominant effect value was-46.1005,and the major gene heritability was 52.47%.The major gene additive effect value of fruit length was 2.456,the dominant effect value was-2.455,and the major gene heritability was 52.52%.The results showed that weight per fruit and fruit length were mainly controlled by a pair of major genes.[Conclusions]This study can provide a theoretical basis for bitter gourd breeding.

Relations of change in fruit and vegetable intake with overall energy reduction and physical activity with weight change:Assessing theory-based psychosocial mediators

Background'.Increased physical activity and fruit and vegetable(FV)intake are typically suggested to counter obesity.Sustained behavior change in those areas has,however,been poor,possibly because of a lack of understanding of the effects of psychosocial factors.Using data from previous research,this study aimed to better define the role of physical activity and FV intake in short;and long-term weight loss via changes in malleable psychosocial mediators and moderators.Methods:Women who were obese(n=183;age=50.0±7.9 years;body mass index=35.2±3.2 kg/m2,mean±SD)and who previously participated in 3 different community-based behavioral weight-loss treatments were assessed over 2 years.Changes in FV intake,physical activity,sei^regulation of physical activity and eating(aggregated),negative mood,and weight were measured over 6 and 24 months.Results'.Changes in each variable over both 6 and 24 months were significant(all p values<0.001).The FV intake to the weight-related caloric intake relationship(β=.0.30,p<0.001)was significantly mediated by selfregulation change over 6 months.The physical activity to weightchange relationship(β=.0.46,p<0.001)was significantly mediated by both sei±regulation and mood change over 24 months.Physical activity,itself,accounted for only 16%of weight-related caloric expenditure differences.Changes in physical activity and FV intake were each independent predictors of weight change over 24 months(overall R2=0.50,p<0.001).Conclusion-.Findings clarified theory-based targets for weight-management treatments and suggested that future weight-loss treatments strongly focus on developing self-regulatory skills to address barriers to behavioral changes.

Changes in fat but not fruit and vegetable intakes linked with body weight change in Mexican women immigrants in Quebec

The objective of the present study was to identify dietary parameters for predicting body weight change (ΔBW) in Mexican-born women (Mexicans) following immigration to Quebec City, Canada. Methods: Changes in fruit (ΔF), vegetable (ΔV), fruit and vegetable (ΔFV), and fat (ΔFat) intake were assessed according to post-immigration periods (1-5 years, 6-10 years, 11-20 years) using a food frequency questionnaire (FFQ). Anthropometric measures were also conducted in 87 Mexicans (study group) and 88 native-born Quebecers (comparison group) aged 18-65 years. Associations were calculated using full and partial robust regression models adjusting for potential confounders (origin, education, income, age, length of residence in Quebec City). Results: There was no difference in ΔBW between the groups. Body weight (BW) increased significantly in both Mexican (5.5 ± 0.9 kg, P < 0.0001) and Quebec women (4.7 ± 0.8 kg, P < 0.0001). ΔBW was positively correlated with ΔFat (β = 0.03, P = 0.003), but not correlated with origin, ΔF, or ΔV. ΔBW was negatively associated with education (β = –4.33, P = 0.007) and positively associated with length of residence (β = 0.3, P = 0.003). Partial models indicated ΔF (β = –1.35, P β = –1.04, P = 0.0001), and ΔFV (β = –2.27, P < 0.0001) were associated with origin, whereas net annual household income (β = 0.16, P = 0.04) was positively associated with ΔFat. Conclusions: Change in body weight could be predicted by length of residence, education, and change in fat intake in Mexican immigrant women and native-born Quebecers whereas changes in fruit and vegetable intakes could be predicted by Mexican or Quebec origin.

Effect of Fertilization in Organic Nursery for Later Growth and Fruiting of Apple Trees in the Orchard

There is a deficiency of nurseries in the world producing fruit trees using the organic material management. However, such material has been produced since 2009 at the Research Institute of Horticulture in Skierniewice. To stimulate plant growth, various biopreparations were applied in an organic nursery. Apple trees （＇Topaz＇/M26） obtained from the organic nursery were used to establish an orchard in 2011. One of the combinations in the orchard consisted of trees produced in a conventional nursery. Trees whose maidens had not been fertilized at all in the organic nursery served as the control. The study was conducted for four years （2011-2014）. The least vigorously growing trees were the control trees and those that had been treated in the nursery with the preparation Tytanit, and the most vigorously--those that had been treated there with manure and the biopreparations Micosat, Humus Active ＋ Aktywit PM, and BF Quality and BF Amin. Trees whose maidens had been produced by the conventional method did not differ, in terms of growth vigour, from those treated with the biopreparations Humus UP or Vinassa. The highest cumulative fruit yields from the four-year-old trees were obtained in the combinations where the maidens had been treated in the nursery with the biopreparations BF Amin and BF Quality. The type of fertilization applied to maiden trees in the nursery had no significant effect on mean fruit weight, but it influenced the distribution of fruit in size classes.

Fruit Characteristics and Mineral Nutrient Concentrations Depending on Different Sizes of “Fuyu” Persimmon Fruits

This study investigated the relationships between fruit size and other fruit quality components in “Fuyu” persimmon. The flower buds were thinned to leave one or two buds per bearing shoot in mid-May. All fruits were harvested on November 12 and they were divided into six size classes by fresh weight. The length/diameter (L/D) ratio and flesh firmness significantly decreased as fruit size increased. Large fruits had a redder skin color with more seeds than small ones. There was a strong negative relationship between fruit size and the L/D ratio (R2 = 0.741, p ≤ 0.01) and firmness (R2 = 0.604, p ≤ 0.01). Skin color and number of seeds per fruit were positively related to fruit size, especially skin color where the relationship was highly positive (R2 = 0.771, p ≤ 0.01). There was a weak, but non-significant positive relation between fruit size and soluble solids. P, K, and Ca concentrations significantly decreased with increasing fruit size. Highly negative relationships were found between fruit size and fruit P (R2 = 0.393, p ≤ 0.01), K (R2 = 0.446, p ≤ 0.01), and Ca (R2 = 0.417, p ≤ 0.01) concentrations. Fruit N and Mg concentrations were not affected by fruit size. It was concluded that fruit size is related to shape, coloration, flesh firmness, and number of seeds per fruit, affecting concentrations of some mineral nutrients in fruits.

Effect of Pre-Harvest Application of Gibberellic Acid on Fruit Quality and Shelf Life of Mandarin (Citrus reticulata Blanco)

Mandarin (Citrus reticulata Blanco) is a promising fruit crop gaining popularity for its human nutrition and economic importance in Nepal. The qualitative losses during pre-harvest stage hinder the productivity and subsequently shorten the on-tree storability. An experiment was conducted to assess the effect of gibberellic acid on quality and shelf life of the mandarin fruit. GA3 at 10, 20, and 30 ppm as against of control were evaluated. Observations on fruit weight (g), fruit firmness (kg/cm2), rind colour (1 - 5 index), juice recovery (%), TSS/acid ratio, PLW (%), decay loss (%), and ascorbic acid (mg/100 ml) were recorded at three harvesting dates i.e. 20 Nov, 5 Dec, and 20 Dec and storage condition. It has been revealed that the fruits treated with GA3 at 20 ppm retained higher fruit weight (128.6 g), more firmness (3.54 kg/cm2), better juice recovery (57.75%), and greater TSS/acid ratio (21.24) at the end of study (20 December). The PLW was found less with GA3 at 30 ppm in both ambient (5.17%) and cellar (6.69%) condition as against untreated fruits (9.52% and 11.76%). Similarly, the decay loss was minimum in the fruits treated with GA3 at 30 ppm both with ambient (1.02%) and cellar condition (8.21%) as against control with ambient (5.54%) and cellar (21.58%).

基施黄腐酸钾对大棚杨梅营养生长及果实品质的影响

以18年生盛果期大棚东魁杨梅为材料,设置秋季基施矿物源黄腐酸钾500 g/株处理和对照(清水),测定植株营养生长和果实性状指标,分析黄腐酸钾对大棚杨梅的影响。结果表明,与对照(清水)相比,黄腐酸钾处理的春梢长度、叶片长度、10片叶厚和叶绿素SPAD值分别增加36.16%、13.06%、26.23%和0.77%,春梢长度、叶长、10片叶厚与对照(清水)差异显著;单果质量、可溶性固形物、总糖、维生素C、总黄酮和花青素含量分别增加6.03%、3.73%、5.74%、5.32%、2.11%和6.90%,总酸含量降低9.42%,除总黄酮含量外,其他果实品质指标均差异显著。说明基施黄腐酸钾能有效促进大棚东魁杨梅营养生长,明显改善果实品质,具有良好的推广应用价值。

甜瓜控制单果重基因的精细定位及候选基因分析

轻果型材料1244(单果重22.59 g)和重果型材料MS-5(单果重814.97 g)配置杂交组合后获得F_(2∶3)分离群体,采用插入缺失(InDel)标记对控制单果重性状的主效QTL位点sfw2.2进行精细定位,进一步对定位区间内的注释基因进行分析,以确定关键候选基因。结果表明:控制单果重性状的主效QTL位点sfw2.2定位在标记CY InDel11和CY InDel16之间,包含3个已知功能基因。其中,纤维蛋白合成相关基因MELO3C029669的相对表达量在两个亲本间存在显著差异,推测其为控制甜瓜单果重的关键基因。根据基因在亲本间的序列差异,开发特异性InDel分子标记BCYInDel13对30份甜瓜材料苗期进行单果重性状的检测,检测准确率可达到90%,具有较高的准确性。

不同授粉方式对“台农1号”百香果果实性状的影响

生产上发现授粉方式对百香果发育及果实性状产生较大影响,为揭示其具体影响方式,以“台农1号”紫果百香果为材料,设置异株异花、同株异花、自花授粉3种授粉方式,测定比较不同授粉方式的果实成熟时间、着果率、果实外观性状、内在品质和种子性状等,并分析各性状指标间的相关性。结果表明,异株异花授粉和自花授粉着果率较高,分别达到98.3%和96.7%;不同授粉方式的单果质量和单果种子数存在显著性差异,从高到低依次为自花授粉>异株异花授粉>同株异花授粉;自花授粉和异株异花授粉可食率较高,而自花授粉果实纵径、横径最大。单果质量与果实横径均呈显著正相关,果实横径与果实纵径也呈极显著正相关;异株异花授粉和同株异花授粉果实单果质量与单果种子数呈显著正相关;同株异花授粉的果实固酸比与成熟时间呈极显著正相关,与可滴定酸呈极显著负相关。推测不同授粉方式对供试百香果品种果实性状影响较大。

不同因素对油桐果及桐饼粕成分影响研究

废弃桐饼粕所含化学物质可用作天然杀虫杀菌的有机肥料,为实现其高效利用,以云南省丘北县油桐(Vernicia fordii)为研究对象,以采摘时间、树种和树龄为研究因素,通过凯氏定氮法和索氏提取法对油桐果及桐饼粕进行重量分析和成分测定。结果表明:10月26日和11月5日采摘三年桐的果灰和果种子重量高于千年桐,且果壳重量较低,在20年树龄时相对重量占比最佳为37%:28%:35%;10月26日采摘15年树龄的三年桐的果壳及桐饼粕氮含量最高分别为5.04%和44.63%,桐饼粕粗脂肪含量为14.50%。综上,根据不同的树种和树龄优化收获时间可较大幅度提高油桐果的产量和重量,增加油桐的经济价值,为后续油桐果及衍生产品的高效利用和可持续发展提供参考。

3个美国油梨品种在广东湛江引种表现评价

为了解引入国家热带果树种质资源圃(广东省湛江市)的Hass、Tonnage和Wilson 3个美国油梨品种的生育期、果实品质性状和适应性等情况,对3个品种花形态、开花特性、开花授粉受精生理特征和主要果实性状等进行观测和比较分析。结果表明,3个油梨品种在广东省湛江市均能正常开花结果,花期均为2月下旬至4月中旬,花期比原产地短10~20 d,Hass和Tonnage为A型花,Wilson为B型花;Hass、Tonnage和Wilson花粉萌发率分别为87%、75%和82%,开花后3 h为最佳授粉期。Hass果实生理成熟期11月初,Tonnage为8月中旬,Wilson为9月初;果实风味、口感、颜色等性状与引种地基本一致,Wilson平均单果质量达到引种地的2倍多,而Tonnage单果质量只达到引种地的69.5%,Hass单果质量与原产地相近。

珊瑚菜果实形成与内源激素含量的相关性分析

为明确珊瑚菜果实形成时期及其4种内源激素含量的动态变化,分析果实形成与内源激素含量间的关系,采用高效液相色谱法测定珊瑚菜果实发育过程中内源生长素(indole-3-acetic acid,IAA)、玉米素(zeatin,ZT)、赤霉素(gibberellin a3,GA_(3))、脱落酸(abscisic acid,ABA)的含量。结果表明,珊瑚菜果实形成过程可划分为乳熟期、中熟期和完熟期;其纵径和横径均呈现出快-慢-快的增长趋势;含水量表现为先升高后降低;百粒重显著增加,至完熟期达2.66 g。在果实发育过程中,ZT和IAA含量均呈升-降-升-降的变化趋势;GA_(3)含量呈降-升-降的变化,其中在果实完熟末期ABA含量达46.02 ng·g^(-1)。相关性分析表明,ZT含量与果实横径呈显著正相关,与果形指数呈极显著负相关;其他性状间相关性不显著。上述结果初步揭示了珊瑚菜果实形成中各指标变化及其与4种内源激素的关系,为激素调控果实形成和提高粒重提供了理论依据。

贡水白柚果实分级标准研究

果实科学分级对于保证果实品质,提高鲜果附加值,促进贡水白柚产业发展是必不可少的。贡水白柚是宣恩县农业主导产业之一,本研究以随机选取的210份贡水白柚果实为材料,进行果形指标、质构、糖度及其相关性分析。结果表明,贡水白柚全果及果肉质量差异明显,全果质量为734~2 178 g,平均1 128.64 g;果肉质量440~731 g,平均564.61 g,根据全果及果肉质量可分为4级。全果质量、果肉质量及横径与果皮顶距、底距、中距、纵径、糖度等各性状指标呈明显的相关关系,可作为主要分级指标。因此,建议将贡水白柚全果质量作为主要分级指标,果实横径作为第二分级指标进行综合分级,对提高贡水白柚的经济效益意义重大。

基于果实重量的草莓品种遗传多样性SSR分析

利用SSR分子标记技术对173份草莓品种进行遗传分析,结果表明:草莓的平均期望杂合度(0.5898)高于平均期望纯合度(0.4102),但是平均观测纯合度(0.8515)却远高于平均观测杂合度(0.1485),纯合基因较多,杂合基因较少,遗传多样性较低。草莓的果重大小与其等位基因有关,纯和的等位基因A、F过高会抑制草莓果实的成长,纯和的等位基因B、C、D过高会促进草莓果实的成长。

蓝靛果忍冬不同品种生长发育习性变化分析

该试验以蓝靛果忍冬‘蓓蕾’‘蓝精灵’和‘乌蓝’3个品种为研究对象,以其新梢生长、果实纵横径、单果鲜重及果实口感评价为指标,对蓝靛果忍冬不同品种生长发育情况进行分析研究。结果表明,蓝靛果忍冬各品种新梢生长和果实发育有较大差异,经综合分析,从好到次的排序为‘蓝精灵’‘乌蓝’‘蓓蕾’。蓝靛果忍冬各品种新梢生长、果实发育高峰期也不尽相同,新梢生长高峰期有两次,第1次在4月中旬,第2次在4月末至5月中旬;果实纵横径生长高峰期在5月中旬;单果鲜重增长高峰期在5月中旬至6月初。因此,在蓝靛果忍冬栽培管理中,应当在新梢和果实生长高峰期加强果园肥水管理,促进果实发育,达到高产,获得最大的经济效益。

基于惯性权重调整的果蝇优化算法在WSN中的应用

目的:针对无线传感器网络随机部署节点的区域覆盖和传感器节点能量消耗问题,提出一种基于惯性权重余弦自适应调整策略的改进果蝇优化算法。方法:该算法在果蝇优化算法基础上,通过引入惯性权重的学习因子调整策略,在线调整算法的搜索步长。结果:增强了果蝇个体的自适应性及全局搜索能力,从而实现全局最优。结论:仿真实验表明,提出的改进果蝇优化算法不仅提高了收敛速度和全局搜索能力,还显著提升了WSN的覆盖率。

人工授粉对火龙果果实发育的影响

于开花期对红皮红肉类型火龙果进行人工授粉与自然授粉处理,并对不同发育阶段的子房或果实进行解剖观察,以研究人工授粉对果实发育的影响。结果表明:人工授粉可提高火龙果的平均单果重和商品果率,平均单果重与果实内种子数呈显著正相关关系;火龙果子房为多胚珠结构,子房内有若干"珠柄-胚珠"单元,胚珠在开花后发育成种子,珠柄则发育成肉质种柄,果实的主要食用部分是由若干个"肉质种柄-种子"单元构成;人工授粉处理使"珠柄-胚珠"单元发育成"肉质种柄-种子"单元的数量增加从而提高单果重。

桃单果重与6个物候期性状的遗传关联分析

【目的】关联分析作为传统连锁分析方法的有效补充,可以鉴定果树的数量性状位点(QuantitativeTrait Loci,QTLs)。本研究通过关联分析定位桃单果重及6个物候期性状的QTLs,以研究性状间的遗传相关,为提高桃品质育种的效率奠定理论基础。【方法】以来源于中国6个生态群的104份桃地方品种为试材,利用分布于桃8条连锁群上的53对SSR(Simple Sequence Repeats)引物,用STRUCTURE 2.3.3和TASSEL 2.0.1软件分别对群体结构和全基因组SSR位点间的连锁不平衡(Linkage Disequilibrium,LD)状况进行分析。在结合单果重和6个物候期性状表型数据后进行关联分析,以定位各性状的QTLs。【结果】群体结构显示供试的104份桃地方品种基于数学模型可以分为5群;LD分析表明在53个SSR位点组成的1378个成对组合中,23个成对位点存在着显著LD且得到统计概率支持。虽然相关性分析表明单果重只与展叶期显著相关,但关联分析却显示单果重不仅与盛花期、展叶期、果实成熟期和落叶期均具有相同的关联位点;而且桃单果重的关联位点与不同连锁群上盛花期、果实发育期和落叶期的关联位点也存在显著的LD现象。对单果重具有最大增效表型效应的等位变异UDP96-013-206同时具有提前花期1.46 d、延迟果实成熟期13.77 d、延迟落叶期2.17 d的表型效应。【结论】本研究得到27个与桃单果重及6个物候期性状关联的QTLs,部分位点与前人定位的连锁群相同。关联分析表明单果重与盛花期、展叶期、果实成熟期、果实发育期和落叶期确实存在遗传相关,主要表现为这几个性状可能受到基因多效性调控或者受连锁群内及连锁群间存在连锁或关联关系的基因调控。