In order to investigate the mechanism of progestin and antiprogestin in the regulation of ovarian steroidogenesis, a dual chamber culture system was prepared with the amnion membrane of human placenta. Isolated porci...In order to investigate the mechanism of progestin and antiprogestin in the regulation of ovarian steroidogenesis, a dual chamber culture system was prepared with the amnion membrane of human placenta. Isolated porcine granulosa and thecal cells from 4~6 mm diameter follicles were grown on both sides of the amnion, respectively, and co cultured with or without LNG and RU486. After 48 h incubation, the mRNAs of FSH receptor (FSH R) and LH receptor (LH R) of both cells were observed by in situ hybridization. The results showed that granulosa cells expressed both FSH R mRNA and LH R mRNA, while thecal cells expressed LH R mRNA only. Under the stimulation of FSH, both LNG and RU486 increased FSH R mRNA expression of granulosa cells. Under the stimulation of LH, LNG enhanced LH R mRNA expression of thecal cells; while RU486 decreased its expression. When granulosa and thecal cells were exposed to FSH and LH both, the actions of LNG and RU486 in thecal cells showed the same result as that stimulated by LH alone. In granulosa cells LNG decreased LH R mRNA expression, while RU486 increased its expression. These data suggest that: (1) granulosa cells expressed FSH R mRNA significantly; (2) both the progestin and antiprogestin directly acted on the mRNA expression of gonadotropin receptors of ovarian cells, but effects were different; (3) the response of granulosa or thecal cells to the action of LNG and RU486 was not the same. The mechanism needs to be further investigated.展开更多
A 19 years old patient with primary amenorrhea was referred to our center. Based on discrepancy between high follicle stimulating hormone (FSH) level and normal ovarian reserve parameters, follicle stimulating hormone...A 19 years old patient with primary amenorrhea was referred to our center. Based on discrepancy between high follicle stimulating hormone (FSH) level and normal ovarian reserve parameters, follicle stimulating hormone receptor (FSHR) mutation was screened. The patient was homozygous in exon 6 of the FSHR gene for the new variant c.479T > C and predicted to result in an aminoacid substitution p.Ile160Thr. One year later, her anti-müllerian hormone (AMH) level inexplicably decreased. Oocyte vitrification was thus offered for fertility preservation. After 17 days of recombinant follicle stimulating hormone (recFSH) (900 IU daily), no follicular growth was seen and estradiol levels remained low. In vitro maturation (IVM) was then suggested. Ten oocytes were successfully vitrified.展开更多
Follicle stimulating hormone (FSH) is a kind of glycoprotein gonadotropin, and plays an important role in the diagnosis and treatment of infertility. Follicle stimulating hormone receptor (FSHR) is a kind of G protein...Follicle stimulating hormone (FSH) is a kind of glycoprotein gonadotropin, and plays an important role in the diagnosis and treatment of infertility. Follicle stimulating hormone receptor (FSHR) is a kind of G protein coupled receptor (GPCR), found in the ovary and testes, and its activation is required for the hormonal operation during the breeding period. In this study, an experimental model of FSHR mediated FSH into cell membrane, which exhibited a phenomenon of fluorescent localized on cell surfaces internalized into cell interior, was established to verify biological activity of FSH.展开更多
文摘In order to investigate the mechanism of progestin and antiprogestin in the regulation of ovarian steroidogenesis, a dual chamber culture system was prepared with the amnion membrane of human placenta. Isolated porcine granulosa and thecal cells from 4~6 mm diameter follicles were grown on both sides of the amnion, respectively, and co cultured with or without LNG and RU486. After 48 h incubation, the mRNAs of FSH receptor (FSH R) and LH receptor (LH R) of both cells were observed by in situ hybridization. The results showed that granulosa cells expressed both FSH R mRNA and LH R mRNA, while thecal cells expressed LH R mRNA only. Under the stimulation of FSH, both LNG and RU486 increased FSH R mRNA expression of granulosa cells. Under the stimulation of LH, LNG enhanced LH R mRNA expression of thecal cells; while RU486 decreased its expression. When granulosa and thecal cells were exposed to FSH and LH both, the actions of LNG and RU486 in thecal cells showed the same result as that stimulated by LH alone. In granulosa cells LNG decreased LH R mRNA expression, while RU486 increased its expression. These data suggest that: (1) granulosa cells expressed FSH R mRNA significantly; (2) both the progestin and antiprogestin directly acted on the mRNA expression of gonadotropin receptors of ovarian cells, but effects were different; (3) the response of granulosa or thecal cells to the action of LNG and RU486 was not the same. The mechanism needs to be further investigated.
文摘A 19 years old patient with primary amenorrhea was referred to our center. Based on discrepancy between high follicle stimulating hormone (FSH) level and normal ovarian reserve parameters, follicle stimulating hormone receptor (FSHR) mutation was screened. The patient was homozygous in exon 6 of the FSHR gene for the new variant c.479T > C and predicted to result in an aminoacid substitution p.Ile160Thr. One year later, her anti-müllerian hormone (AMH) level inexplicably decreased. Oocyte vitrification was thus offered for fertility preservation. After 17 days of recombinant follicle stimulating hormone (recFSH) (900 IU daily), no follicular growth was seen and estradiol levels remained low. In vitro maturation (IVM) was then suggested. Ten oocytes were successfully vitrified.
文摘Follicle stimulating hormone (FSH) is a kind of glycoprotein gonadotropin, and plays an important role in the diagnosis and treatment of infertility. Follicle stimulating hormone receptor (FSHR) is a kind of G protein coupled receptor (GPCR), found in the ovary and testes, and its activation is required for the hormonal operation during the breeding period. In this study, an experimental model of FSHR mediated FSH into cell membrane, which exhibited a phenomenon of fluorescent localized on cell surfaces internalized into cell interior, was established to verify biological activity of FSH.
