The FTZ-F1 genes encode orphan receptors of the nuclear receptor superfamily and in mammals have been found to play important roles in the proper development of the adrenal-gonadal axis and sex-determination.We isolat...The FTZ-F1 genes encode orphan receptors of the nuclear receptor superfamily and in mammals have been found to play important roles in the proper development of the adrenal-gonadal axis and sex-determination.We isolated the homologue of FTZ-F1 in genetically improved farmed tilapia(gfFTZ-F1).The full-length cDNA was isolated from the ovary,which included an open reading frame encoding a predicted protein of 486 amino acids.Sequence,tissue distribution and phylogenic analysis of the FTZ-F1 showed that the gfFTZ-F1 belonged to SF-1/Ad4BP group and that gfFTZ-F1 transcripts were only expressed in the gonads and kidney but not in other tissues.Likewise our data suggests that the gfFTZ-F1 gene may share similar functions with other fish and mammalian counterparts,though further study is needed to make any definitive conclusions.展开更多
Sex related FTZ-F1 is a transcriptional factor regulating the expression of fushi tarazu (a member of the orphan nuclear receptors) gene. In this study, FTZ-F1 gene (FTZ-F1) was isolated from the testis of black rockf...Sex related FTZ-F1 is a transcriptional factor regulating the expression of fushi tarazu (a member of the orphan nuclear receptors) gene. In this study, FTZ-F1 gene (FTZ-F1) was isolated from the testis of black rockfish (Sebastes schlegeli) by homology cloning. The full-length cDNA of S. schlegeli FTZ-F1 (ssFTZ-F1) contained a 232bp 5′UTR, a 1449bp ORF encoding FTZ-F1 (482 amino acid residules in length) with an estimated molecular weight of 5.4kD and a 105bp 3′UTR. Sequence, tissue distribution and phylogenic analysis showed that ssFTZ-F1 belonged to FTZ group, holding highly conserved regions including Ⅰ, Ⅱ and Ⅲ FTZ-F1 boxes and an AF-2 hexamer. Relatively high expression was observed at different larva stages. In juveniles (105 days old), the transcript of ssFTZ-F1 can be detected in all tissues and the abuncance of the gene transcript in testis, ovary, spleen and brain was higher than that in other tissues. In mature fish, the abundance of gene transcript was higher in testis, ovary, spleen and brain than that in liver (trace amount), and the gene was not transcribed in other tissues. The highest abundance of gene transcript was always observed in gonads of both juvenile and mature fish. In addition, the abundance of gene transcript in male tissues were higher than that in female tissue counterparts (P<0.05).展开更多
Though located in China's hinterland,Xinjiang Uygur Autonomous Region is becoming a new frontier of opening up.The China(Xinjiang)Pilot Free Trade Zone(FTZ)was officially inaugurated on November 1,making it the 22...Though located in China's hinterland,Xinjiang Uygur Autonomous Region is becoming a new frontier of opening up.The China(Xinjiang)Pilot Free Trade Zone(FTZ)was officially inaugurated on November 1,making it the 22nd FTZ in China and the first in the country's northwestern border regions.展开更多
The China(Shanghai)Pilot Free Trade Zone(FTZ),the first of its kind in the country,was launched in Shanghai’s Pudong New Area in September 2013,marking a trial run for groundbreaking changes to free up cross-border c...The China(Shanghai)Pilot Free Trade Zone(FTZ),the first of its kind in the country,was launched in Shanghai’s Pudong New Area in September 2013,marking a trial run for groundbreaking changes to free up cross-border commodity and capital flows in China.It originally consisted of the Waigaoqiao FTZ,the Waigaoqiao Free Trade Logistics Park,the Yangshan Free Trade Port Area and the Pudong Airport FTZ.展开更多
基金Supported by grants from State 863 High-Technology R&D Project of China(2006AA10A403)Shandong Genetic Improvement Key Project for Agricultural OrganismDoctor Initial Funding of Guangdong Ocean University(0712103)
基金the Natural Science Foundation of Shanxi Province,China (2009021038)the Doctor Initial Foundation of Shanxi Agricultural University (XB2009003)+2 种基金the Science and Technology Innovational Foundation of Shanxi Agricultural University (2009005)the Nonprofit Research Institutes,Special Foundation for Operating Expenses of Basic Research Projects of Guangxi Zhuang Autonomous Region(2060302 GXIF-2008-01)the Scientific Research and Technological Development Projects of Nanning City(200801016B)
文摘The FTZ-F1 genes encode orphan receptors of the nuclear receptor superfamily and in mammals have been found to play important roles in the proper development of the adrenal-gonadal axis and sex-determination.We isolated the homologue of FTZ-F1 in genetically improved farmed tilapia(gfFTZ-F1).The full-length cDNA was isolated from the ovary,which included an open reading frame encoding a predicted protein of 486 amino acids.Sequence,tissue distribution and phylogenic analysis of the FTZ-F1 showed that the gfFTZ-F1 belonged to SF-1/Ad4BP group and that gfFTZ-F1 transcripts were only expressed in the gonads and kidney but not in other tissues.Likewise our data suggests that the gfFTZ-F1 gene may share similar functions with other fish and mammalian counterparts,though further study is needed to make any definitive conclusions.
基金supported by the National High-Tech Research and Development Program (2012AA10A402)the National Natural Science Foundation of China(31172385)
文摘Sex related FTZ-F1 is a transcriptional factor regulating the expression of fushi tarazu (a member of the orphan nuclear receptors) gene. In this study, FTZ-F1 gene (FTZ-F1) was isolated from the testis of black rockfish (Sebastes schlegeli) by homology cloning. The full-length cDNA of S. schlegeli FTZ-F1 (ssFTZ-F1) contained a 232bp 5′UTR, a 1449bp ORF encoding FTZ-F1 (482 amino acid residules in length) with an estimated molecular weight of 5.4kD and a 105bp 3′UTR. Sequence, tissue distribution and phylogenic analysis showed that ssFTZ-F1 belonged to FTZ group, holding highly conserved regions including Ⅰ, Ⅱ and Ⅲ FTZ-F1 boxes and an AF-2 hexamer. Relatively high expression was observed at different larva stages. In juveniles (105 days old), the transcript of ssFTZ-F1 can be detected in all tissues and the abuncance of the gene transcript in testis, ovary, spleen and brain was higher than that in other tissues. In mature fish, the abundance of gene transcript was higher in testis, ovary, spleen and brain than that in liver (trace amount), and the gene was not transcribed in other tissues. The highest abundance of gene transcript was always observed in gonads of both juvenile and mature fish. In addition, the abundance of gene transcript in male tissues were higher than that in female tissue counterparts (P<0.05).
文摘Though located in China's hinterland,Xinjiang Uygur Autonomous Region is becoming a new frontier of opening up.The China(Xinjiang)Pilot Free Trade Zone(FTZ)was officially inaugurated on November 1,making it the 22nd FTZ in China and the first in the country's northwestern border regions.
文摘The China(Shanghai)Pilot Free Trade Zone(FTZ),the first of its kind in the country,was launched in Shanghai’s Pudong New Area in September 2013,marking a trial run for groundbreaking changes to free up cross-border commodity and capital flows in China.It originally consisted of the Waigaoqiao FTZ,the Waigaoqiao Free Trade Logistics Park,the Yangshan Free Trade Port Area and the Pudong Airport FTZ.
