Objective To investigate the effects of different fractions from Fuke Qianjin Formula(妇科千金方,FKQJF)on uterine leiomyoma(UL)to determine the best fraction.Methods FKQJF was extracted and isolated to obtain polysacc...Objective To investigate the effects of different fractions from Fuke Qianjin Formula(妇科千金方,FKQJF)on uterine leiomyoma(UL)to determine the best fraction.Methods FKQJF was extracted and isolated to obtain polysaccharides(FKP),flavonoids(FKF),and grease(FKG).140 female SPF SD rats were divided into 14 groups[model(MOD),normal control(NC),Gouliuqing(GLQ),Mifepristone(MFST),FKQJF,low,medium,and high dose of polysaccharides(l-FKP,m-FKP,and h-FKP),low,medium,and high dose of flavonoids(l-FKF,m-FKF,and h-FKF),low,medium,and high dose of grease(l-FKG,m-FKG,and h-FKG)],and uterine fibroids model rats were treated with drugs for four weeks.Serum levels of estrogen and progesterone were measured using enzyme-linked immunoassay assay(ELISA)kits.The expression of estrogen receptor(ER-α,ER-β)and progesterone receptor(PR)in the uterus was observed using immunohistochemistry(IHC).Serum metabolite profiles and FKG were analyzed using gas chromatography-mass spectrometry(GC-MS).Results FKQJF,h-FKF,m-FKG,and h-FKG significantly downregulated the estrogen level in the uterine fibroid model rats(P<0.01).FKQJF,h-FKF,and h-FKG significantly reduced the level of progesterone in the uterine fibroid model rats(P<0.01).The levels of ER-α,ER-β,and PR in uterine fibroid model rats were significantly decreased by FKQJF and h-FKG(P<0.01).The levels of ER-α,ER-β,and PR in the fibroid model rats were decreased by m-FKG(P<0.05).Additionally,serum metabolism results revealed that h-FKG and FKQJF could regulate related endogenous metabolites and make the pathological indices of uterine fibroids in rats close to the normal group.Forty-six components were identified in the oil,accounting for 91.97%of the total oil components.Conclusion FKQJF and h-FKG showed a significant anti-myoma activity and significantly improved the pathological state of the uterus in rats with hysteromyoma.The mechanism of action may be related to the regulation of estrogen progesterone and its receptor in uterine fibroid model animals.These findings proved the effect of FKQJF on uterine leiomyoma and provided an experimental basis for its clinical research and application.展开更多
Objective:To systematically evaluate the efficacy and safety of Fuke Qianjin Tablets(妇科千金片)in the treatment of chronic pelvic inflammation.Methods:A systematically and comprehensively search was conducted in 4 Ch...Objective:To systematically evaluate the efficacy and safety of Fuke Qianjin Tablets(妇科千金片)in the treatment of chronic pelvic inflammation.Methods:A systematically and comprehensively search was conducted in 4 Chinese databases of CNKI,VIP,Wan Fang and CBM and the foreign language databases of Pubmed,EMbase and The Cochrane Library.The retrieval time was from database establishment to March 2019.The randomized controlled trials of Fuke Qianjin Tablets(妇科千金片)in the treatment of chronic pelvic inflammation were selected according to the predetermined criteria.The quality of the included study was evaluated by Cochrane collaborative network bias risk evaluation tool,and the meta-analysis was performed by Rev Man5.3 software.Results:A total of 1009 related literatures were searched.After initial screening and strict evaluation,55 studies were included,with a total sample size of 6826 cases,including 3416 cases in the experiment group and 3410 cases in the control group.The results of meta-analysis showed that the total effective rate of Fuke Qianjin Tablets(妇科千金片)combined with antibiotics in the treatment of chronic pelvic inflammation was better than that of antibiotics alone(RR=1.20,95%CI[1.17,1.22],P<0.00001).Fuke Qianjin Tablets(妇科千金片)combined with antibiotics was better than that of antibiotics alone in the improvement of abdominal pain symptoms(RR=1.40,95%CI[1.04,1.88],P<0.00001),leukorrhea abnormality(RR=1.38,95%CI[1.16,1.65],P<0.0004).In terms of safety,Fuke Qianjin Tablets(妇科千金片)combined with antibiotics could reduce the incidence of adverse reactions(RR=0.67,95%CI[0.48,0.93],P<0.02).The main adverse reactions were nausea and vomiting,bitterness and astringency in the mouth,rash and so on.All of them could be tolerated and the symptoms could disappear in the short term,and had no effect on the treatment.Conclusion:Fuke Qianjin Tablets(妇科千金片)combined with antibiotics in the treatment of chronic pelvic inflammation can improve the total effective rate,relieve abdominal pain and abnormal leukorrhea and other clinical discomfort symptoms,improve the quality of life of patients to a certain extent,and no serious adverse reactions are found.Due to the limitation of the quality and quantity of the included literature,the above conclusions need to be further studied and verified by high-quality research.展开更多
Objective:To explore effect of bock greenbrier rhizome capsule combined with Fuke Qianjin Tablet on serum inflammatory factors, immune function and hemorheological index in patients with chronic pelvic inflammation.Me...Objective:To explore effect of bock greenbrier rhizome capsule combined with Fuke Qianjin Tablet on serum inflammatory factors, immune function and hemorheological index in patients with chronic pelvic inflammation.Method:A toal of210 cases of patients with chronic pelvic inflammation were divided into control group (n=104) and observation group (n=106) according to random data table method, control group was given Fuke Qianjin Tablet, observation group was given bock greenbrier rhizome capsule on base of control group, treatment period of both groups was 6 weeks. Compared serum inflammatory factors, immune function and hemorheological index in both groups before and after 6 weeks treatment. Results:Before treatment, comparison of inflammatory factors (TNF-α, IL-6 and IL-8), immune function (CD3+, CD4+, CD8+ and CD4+/CD8+) and hemorheological index (low-cut/high-cut whole blood viscosity, plasma viscosity, FIB) in both groups, there was no statistical significant difference;after treatment, TNF-α, IL-6, IL-8, CD8+, low-cut/ high-cut whole blood viscosity, plasma viscosity, FIB level were lower dramatically than pre-treatment intra-group, moreover, these level in observation group after treatment was lower than control group after treatment;CD3+, CD4+ and CD4+/CD8+ level in both groups after treatment was higher than before treatment intra-group, and these levels in observation group after treatment was higher obviously than control group after treatment.Conclusion:Bock greenbrier rhizome capsule combined with Fuke Qianjin Tablet for patients with chronic pelvic inflammation, its regulated effect on serum inflammatory factors, immune function and hemorheological index in patients with chronic pelvic inflammation was superior to only using Fuke Qianjin Tablet, it was worthy of clinical application.展开更多
基金funding support from the Major Science and Technology Projects in Hunan Province(No.2015SK1001)Special Funds from the Central Government to Guide Local Science and Technology Development(No.2019XF5076)+1 种基金Natural Science Foundation of Hunan Province(No.2017JJ1023 and No.2019JJ50443)the Research project of Hunan Education Department(No.17B198 and No.19C1398)。
文摘Objective To investigate the effects of different fractions from Fuke Qianjin Formula(妇科千金方,FKQJF)on uterine leiomyoma(UL)to determine the best fraction.Methods FKQJF was extracted and isolated to obtain polysaccharides(FKP),flavonoids(FKF),and grease(FKG).140 female SPF SD rats were divided into 14 groups[model(MOD),normal control(NC),Gouliuqing(GLQ),Mifepristone(MFST),FKQJF,low,medium,and high dose of polysaccharides(l-FKP,m-FKP,and h-FKP),low,medium,and high dose of flavonoids(l-FKF,m-FKF,and h-FKF),low,medium,and high dose of grease(l-FKG,m-FKG,and h-FKG)],and uterine fibroids model rats were treated with drugs for four weeks.Serum levels of estrogen and progesterone were measured using enzyme-linked immunoassay assay(ELISA)kits.The expression of estrogen receptor(ER-α,ER-β)and progesterone receptor(PR)in the uterus was observed using immunohistochemistry(IHC).Serum metabolite profiles and FKG were analyzed using gas chromatography-mass spectrometry(GC-MS).Results FKQJF,h-FKF,m-FKG,and h-FKG significantly downregulated the estrogen level in the uterine fibroid model rats(P<0.01).FKQJF,h-FKF,and h-FKG significantly reduced the level of progesterone in the uterine fibroid model rats(P<0.01).The levels of ER-α,ER-β,and PR in uterine fibroid model rats were significantly decreased by FKQJF and h-FKG(P<0.01).The levels of ER-α,ER-β,and PR in the fibroid model rats were decreased by m-FKG(P<0.05).Additionally,serum metabolism results revealed that h-FKG and FKQJF could regulate related endogenous metabolites and make the pathological indices of uterine fibroids in rats close to the normal group.Forty-six components were identified in the oil,accounting for 91.97%of the total oil components.Conclusion FKQJF and h-FKG showed a significant anti-myoma activity and significantly improved the pathological state of the uterus in rats with hysteromyoma.The mechanism of action may be related to the regulation of estrogen progesterone and its receptor in uterine fibroid model animals.These findings proved the effect of FKQJF on uterine leiomyoma and provided an experimental basis for its clinical research and application.
基金National Key Research and Development Plan Project(2018YFC1707400)Basic Business Expense Project of Central Public Welfare Research Institutes(Z0605)。
文摘Objective:To systematically evaluate the efficacy and safety of Fuke Qianjin Tablets(妇科千金片)in the treatment of chronic pelvic inflammation.Methods:A systematically and comprehensively search was conducted in 4 Chinese databases of CNKI,VIP,Wan Fang and CBM and the foreign language databases of Pubmed,EMbase and The Cochrane Library.The retrieval time was from database establishment to March 2019.The randomized controlled trials of Fuke Qianjin Tablets(妇科千金片)in the treatment of chronic pelvic inflammation were selected according to the predetermined criteria.The quality of the included study was evaluated by Cochrane collaborative network bias risk evaluation tool,and the meta-analysis was performed by Rev Man5.3 software.Results:A total of 1009 related literatures were searched.After initial screening and strict evaluation,55 studies were included,with a total sample size of 6826 cases,including 3416 cases in the experiment group and 3410 cases in the control group.The results of meta-analysis showed that the total effective rate of Fuke Qianjin Tablets(妇科千金片)combined with antibiotics in the treatment of chronic pelvic inflammation was better than that of antibiotics alone(RR=1.20,95%CI[1.17,1.22],P<0.00001).Fuke Qianjin Tablets(妇科千金片)combined with antibiotics was better than that of antibiotics alone in the improvement of abdominal pain symptoms(RR=1.40,95%CI[1.04,1.88],P<0.00001),leukorrhea abnormality(RR=1.38,95%CI[1.16,1.65],P<0.0004).In terms of safety,Fuke Qianjin Tablets(妇科千金片)combined with antibiotics could reduce the incidence of adverse reactions(RR=0.67,95%CI[0.48,0.93],P<0.02).The main adverse reactions were nausea and vomiting,bitterness and astringency in the mouth,rash and so on.All of them could be tolerated and the symptoms could disappear in the short term,and had no effect on the treatment.Conclusion:Fuke Qianjin Tablets(妇科千金片)combined with antibiotics in the treatment of chronic pelvic inflammation can improve the total effective rate,relieve abdominal pain and abnormal leukorrhea and other clinical discomfort symptoms,improve the quality of life of patients to a certain extent,and no serious adverse reactions are found.Due to the limitation of the quality and quantity of the included literature,the above conclusions need to be further studied and verified by high-quality research.
文摘Objective:To explore effect of bock greenbrier rhizome capsule combined with Fuke Qianjin Tablet on serum inflammatory factors, immune function and hemorheological index in patients with chronic pelvic inflammation.Method:A toal of210 cases of patients with chronic pelvic inflammation were divided into control group (n=104) and observation group (n=106) according to random data table method, control group was given Fuke Qianjin Tablet, observation group was given bock greenbrier rhizome capsule on base of control group, treatment period of both groups was 6 weeks. Compared serum inflammatory factors, immune function and hemorheological index in both groups before and after 6 weeks treatment. Results:Before treatment, comparison of inflammatory factors (TNF-α, IL-6 and IL-8), immune function (CD3+, CD4+, CD8+ and CD4+/CD8+) and hemorheological index (low-cut/high-cut whole blood viscosity, plasma viscosity, FIB) in both groups, there was no statistical significant difference;after treatment, TNF-α, IL-6, IL-8, CD8+, low-cut/ high-cut whole blood viscosity, plasma viscosity, FIB level were lower dramatically than pre-treatment intra-group, moreover, these level in observation group after treatment was lower than control group after treatment;CD3+, CD4+ and CD4+/CD8+ level in both groups after treatment was higher than before treatment intra-group, and these levels in observation group after treatment was higher obviously than control group after treatment.Conclusion:Bock greenbrier rhizome capsule combined with Fuke Qianjin Tablet for patients with chronic pelvic inflammation, its regulated effect on serum inflammatory factors, immune function and hemorheological index in patients with chronic pelvic inflammation was superior to only using Fuke Qianjin Tablet, it was worthy of clinical application.
