Objective To investigate the distribution of single nucleotide polymorphisms (SNPs)in CAPN10 gene in Chinese population and their relation with type 2 diabetes mellitus inHan people of Northern China. Methods CA...Objective To investigate the distribution of single nucleotide polymorphisms (SNPs)in CAPN10 gene in Chinese population and their relation with type 2 diabetes mellitus inHan people of Northern China. Methods CAPN10 gene was sequenced to detect SNPs indifferent nationalities of China. Five SNPs were chosen to perform case-control study andhaplotype analysis in 156 normal Han people of Northern China and 173 type 2 diabetes. OneSNP was also analyzed with transmission-disequilibrium test (TDT) and sib transmission-disequilibrium test (STDT) in 68 type 2 diabetes pedigrees (377 people). Results A totalof 40 SNPs were identified in length of 8 936bp, with an average of 1 in every 223bp. TheSNPs in CAPN10 gene did not distribute evenly and the SNPs in Chinese were different fromthose reported in Mexican American. There was no significantly statistical difference in theallele frequency of the 5 SNPs between case and control, and the haplotype frequencies inthe two groups were not significantly different. No positive results was found in TDT andSTDT analysis. Conclusions The SNP distribution of CAPN10 gene differs in differentnationalities. The studied SNPs in CAPN10 gene may not be the major susceptibility onesof type 2 diabetes mellitus in Han people of Northern China.展开更多
Two eremophilane-type sesquiterpenoids from the rhizomes of Ligularia veitchiana（Hemsl.） Greenm were isolated and determined as 10α,11-endoperoxide-1（2）-en-7α-hydroeremophilane（1） and 1（10）-en-2-oxo-7α-isop...Two eremophilane-type sesquiterpenoids from the rhizomes of Ligularia veitchiana（Hemsl.） Greenm were isolated and determined as 10α,11-endoperoxide-1（2）-en-7α-hydroeremophilane（1） and 1（10）-en-2-oxo-7α-isopropanol-eremophilane（2）.Compound 1 was a new compound and compound 2 was a new natural product.The structures of the two compounds were established by means of spectral methods including 1^H NMR,13^C NMR,2D NMR and MS.展开更多
目的探究血清同型半胱氨酸(Hcy)、解整合素⁃金属蛋白酶10(ADAM10)、半乳糖凝集素3(Gal⁃3)与2型糖尿病(T2DM)患者颈动脉粥样硬化(CAS)的相关性。方法选取云南大学附属医院2021年10月至2022年10月收治的98例T2DM患者为观察组,另根据性别...目的探究血清同型半胱氨酸(Hcy)、解整合素⁃金属蛋白酶10(ADAM10)、半乳糖凝集素3(Gal⁃3)与2型糖尿病(T2DM)患者颈动脉粥样硬化(CAS)的相关性。方法选取云南大学附属医院2021年10月至2022年10月收治的98例T2DM患者为观察组,另根据性别、年龄匹配选取同期体检健康者40名为对照组。检验所有研究对象血清Hcy、ADAM10、Gal⁃3水平,对比2组指标差异,分析各项指标与CAS的关系。结果观察组共45例患者纳入T2DM组,53例纳入T2DM+CAS组,三组研究对象的BMI、FBG、2 h FBG、HbA1c、TC、TG、HDL⁃C、LDL⁃C、Hcy、ADAM10、Gal⁃3及左右侧颈动脉内膜中层厚度(intimal media thickness,IMT)水平比较,差异有统计学意义(F=61.934、67.925、65.751、24.465、305.08、24.638、24.789、133.902、43.027、183.94、305.08、265.287,P<0.05)。T2DM+CAS组患者的TC、LDL⁃C、Hcy、ADAM10、Gal⁃3及左右侧IMT水平均高于T2DM组,差异有统计学意义(t=3.064、12.111、8.168、7.575、8.568、12.111、12.089,P<0.05)。观察组患者左侧IMT与ADAM10存在中等程度相关,与Hcy、Gal⁃3存在强相关(r=0.524、0638、0.609,P<0.05);右侧IMT与Hcy、ADAM10、Gal⁃3存在强相关(r=0.699、0.609、0.693,P<0.05)。回归模型中,Hcy、ADAM10、Gal⁃3均为T2DM患者CAS的危险因素(P<0.05)。结论T2DM患者CAS的发生发展受多方面因素,其中血清Hcy、ADAM10、Gal⁃3水平升高均会加重CAS病情,临床上需要加以重视。展开更多
目的阿卡波糖治疗2型糖尿病的疗效及对患者C反应蛋白(CRP)、白细胞介素-10(IL-10)水平的影响。方法选取2019年1月至2020年10月本院收治的84例2型糖尿病患者作为研究对象,按照随机数字表法分为对比组与研究组,每组42例。对比组给予二甲...目的阿卡波糖治疗2型糖尿病的疗效及对患者C反应蛋白(CRP)、白细胞介素-10(IL-10)水平的影响。方法选取2019年1月至2020年10月本院收治的84例2型糖尿病患者作为研究对象,按照随机数字表法分为对比组与研究组,每组42例。对比组给予二甲双胍治疗,研究组给予阿卡波糖治疗,比较两组治疗前后空腹血糖(FBG)、餐后2 h血糖(2 h PBG)、糖化血红蛋白(HbAlc)、C反应蛋白(CRP)、白细胞介素-10(IL-10)水平及不良反应发生情况。结果治疗前,两组FBG、2 h PBG、HbAlc水平比较差异无统计学意义;治疗后,两组FBG、2 h PBG、HbAlc水平均低于治疗前,且研究组低于对比组,差异有统计学意义(P<0.05)。治疗前,两组CRP、IL-10水平比较差异无统计学意义;治疗后,两组CRP、IL-10水平均低于治疗前,且研究组低于对比组,差异有统计学意义(P<0.05)。研究组不良反应发生率为4.76%,低于对比组的19.05%,差异有统计学意义(P<0.05)。结论阿卡波糖治疗2型糖尿病疗效显著,可明显降低患者血糖水平,抑制炎症因子生成,安全性较高,值得临床广泛应用。展开更多
基金the National Natural Science Foundation (Grant No. 30170441,39896200)863 High Tech Project (Grant No. 2001AA221161)+2 种基金 National 973 Project (Grant No.G1998051016) Beijing Natural Science Fund (Grant No. 7002026)the National High EducationSci
文摘Objective To investigate the distribution of single nucleotide polymorphisms (SNPs)in CAPN10 gene in Chinese population and their relation with type 2 diabetes mellitus inHan people of Northern China. Methods CAPN10 gene was sequenced to detect SNPs indifferent nationalities of China. Five SNPs were chosen to perform case-control study andhaplotype analysis in 156 normal Han people of Northern China and 173 type 2 diabetes. OneSNP was also analyzed with transmission-disequilibrium test (TDT) and sib transmission-disequilibrium test (STDT) in 68 type 2 diabetes pedigrees (377 people). Results A totalof 40 SNPs were identified in length of 8 936bp, with an average of 1 in every 223bp. TheSNPs in CAPN10 gene did not distribute evenly and the SNPs in Chinese were different fromthose reported in Mexican American. There was no significantly statistical difference in theallele frequency of the 5 SNPs between case and control, and the haplotype frequencies inthe two groups were not significantly different. No positive results was found in TDT andSTDT analysis. Conclusions The SNP distribution of CAPN10 gene differs in differentnationalities. The studied SNPs in CAPN10 gene may not be the major susceptibility onesof type 2 diabetes mellitus in Han people of Northern China.
文摘Two eremophilane-type sesquiterpenoids from the rhizomes of Ligularia veitchiana（Hemsl.） Greenm were isolated and determined as 10α,11-endoperoxide-1（2）-en-7α-hydroeremophilane（1） and 1（10）-en-2-oxo-7α-isopropanol-eremophilane（2）.Compound 1 was a new compound and compound 2 was a new natural product.The structures of the two compounds were established by means of spectral methods including 1^H NMR,13^C NMR,2D NMR and MS.
文摘目的探究血清同型半胱氨酸(Hcy)、解整合素⁃金属蛋白酶10(ADAM10)、半乳糖凝集素3(Gal⁃3)与2型糖尿病(T2DM)患者颈动脉粥样硬化(CAS)的相关性。方法选取云南大学附属医院2021年10月至2022年10月收治的98例T2DM患者为观察组,另根据性别、年龄匹配选取同期体检健康者40名为对照组。检验所有研究对象血清Hcy、ADAM10、Gal⁃3水平,对比2组指标差异,分析各项指标与CAS的关系。结果观察组共45例患者纳入T2DM组,53例纳入T2DM+CAS组,三组研究对象的BMI、FBG、2 h FBG、HbA1c、TC、TG、HDL⁃C、LDL⁃C、Hcy、ADAM10、Gal⁃3及左右侧颈动脉内膜中层厚度(intimal media thickness,IMT)水平比较,差异有统计学意义(F=61.934、67.925、65.751、24.465、305.08、24.638、24.789、133.902、43.027、183.94、305.08、265.287,P<0.05)。T2DM+CAS组患者的TC、LDL⁃C、Hcy、ADAM10、Gal⁃3及左右侧IMT水平均高于T2DM组,差异有统计学意义(t=3.064、12.111、8.168、7.575、8.568、12.111、12.089,P<0.05)。观察组患者左侧IMT与ADAM10存在中等程度相关,与Hcy、Gal⁃3存在强相关(r=0.524、0638、0.609,P<0.05);右侧IMT与Hcy、ADAM10、Gal⁃3存在强相关(r=0.699、0.609、0.693,P<0.05)。回归模型中,Hcy、ADAM10、Gal⁃3均为T2DM患者CAS的危险因素(P<0.05)。结论T2DM患者CAS的发生发展受多方面因素,其中血清Hcy、ADAM10、Gal⁃3水平升高均会加重CAS病情,临床上需要加以重视。
文摘目的阿卡波糖治疗2型糖尿病的疗效及对患者C反应蛋白(CRP)、白细胞介素-10(IL-10)水平的影响。方法选取2019年1月至2020年10月本院收治的84例2型糖尿病患者作为研究对象,按照随机数字表法分为对比组与研究组,每组42例。对比组给予二甲双胍治疗,研究组给予阿卡波糖治疗,比较两组治疗前后空腹血糖(FBG)、餐后2 h血糖(2 h PBG)、糖化血红蛋白(HbAlc)、C反应蛋白(CRP)、白细胞介素-10(IL-10)水平及不良反应发生情况。结果治疗前,两组FBG、2 h PBG、HbAlc水平比较差异无统计学意义;治疗后,两组FBG、2 h PBG、HbAlc水平均低于治疗前,且研究组低于对比组,差异有统计学意义(P<0.05)。治疗前,两组CRP、IL-10水平比较差异无统计学意义;治疗后,两组CRP、IL-10水平均低于治疗前,且研究组低于对比组,差异有统计学意义(P<0.05)。研究组不良反应发生率为4.76%,低于对比组的19.05%,差异有统计学意义(P<0.05)。结论阿卡波糖治疗2型糖尿病疗效显著,可明显降低患者血糖水平,抑制炎症因子生成,安全性较高,值得临床广泛应用。
