橡胶树棒孢霉落叶病(Corynespora leaf fall disease,CLFD)是全球主要植胶国最为严重的叶部病害之一,可造成严重的产量和经济损失,而抗病种质鉴选与创制利用是该病最为有效的防治策略。本研究对云研277-5×IAN 873、RRIC103×热...橡胶树棒孢霉落叶病(Corynespora leaf fall disease,CLFD)是全球主要植胶国最为严重的叶部病害之一,可造成严重的产量和经济损失,而抗病种质鉴选与创制利用是该病最为有效的防治策略。本研究对云研277-5×IAN 873、RRIC103×热研8-79和云研277-5×热垦525三个杂交组合的821份F_(1)代群体进行了抗棒孢霉落叶病的评价,明确了F_(1)代群体的抗病性水平,并从符合正态分布的2个杂交组合中筛选出32份候选F_(1)代单株进行芽接,再分别利用3个亚型的多主棒孢病菌和2种评价方法对候选F_(1)代无性系种苗进行抗病性复筛,最终获得5份抗病性较好的F_(1)代新种质。通过对5份抗病新种质防御酶活性的测定,以及抗病相关基因表达特性的分析,进一步证实了5份抗病新种质与多主棒孢病菌在侵染过程中的互作关系,明确了其在病原菌接种不同时间段的差异表达特征。本研究为橡胶树棒孢霉落叶病抗病性早期鉴选、抗病种质培育与创制利用提供了很好的种质材料和理论支撑。展开更多
[Objective]The paper was to provide theoretical basis for selection of parental combination and early identification of hybrids.[Method]The soluble protein and peroxidase of LiLum davidii var.unicolor,Lilium Asiatic h...[Objective]The paper was to provide theoretical basis for selection of parental combination and early identification of hybrids.[Method]The soluble protein and peroxidase of LiLum davidii var.unicolor,Lilium Asiatic hybrids and their filial generations were analyzed using polyacrylamide gel electrophoresis technique.[Result]The protein spectrum of filial generation with L.davidii var.unicolor as parent not only appeared the homologous band as parent with darker coloring,but also had new bands compared with parent.Peroxidase zymogram of hybrid F1 mainly displayed incomplete complementary and hybrid type of parent.[Conclusion]Protein spectrum and peroxidase zymogram could be used as the biochemical markers for the identification of hybrids of lily,which could also detect the target traits of plant.展开更多
文摘橡胶树棒孢霉落叶病(Corynespora leaf fall disease,CLFD)是全球主要植胶国最为严重的叶部病害之一,可造成严重的产量和经济损失,而抗病种质鉴选与创制利用是该病最为有效的防治策略。本研究对云研277-5×IAN 873、RRIC103×热研8-79和云研277-5×热垦525三个杂交组合的821份F_(1)代群体进行了抗棒孢霉落叶病的评价,明确了F_(1)代群体的抗病性水平,并从符合正态分布的2个杂交组合中筛选出32份候选F_(1)代单株进行芽接,再分别利用3个亚型的多主棒孢病菌和2种评价方法对候选F_(1)代无性系种苗进行抗病性复筛,最终获得5份抗病性较好的F_(1)代新种质。通过对5份抗病新种质防御酶活性的测定,以及抗病相关基因表达特性的分析,进一步证实了5份抗病新种质与多主棒孢病菌在侵染过程中的互作关系,明确了其在病原菌接种不同时间段的差异表达特征。本研究为橡胶树棒孢霉落叶病抗病性早期鉴选、抗病种质培育与创制利用提供了很好的种质材料和理论支撑。
文摘[Objective]The paper was to provide theoretical basis for selection of parental combination and early identification of hybrids.[Method]The soluble protein and peroxidase of LiLum davidii var.unicolor,Lilium Asiatic hybrids and their filial generations were analyzed using polyacrylamide gel electrophoresis technique.[Result]The protein spectrum of filial generation with L.davidii var.unicolor as parent not only appeared the homologous band as parent with darker coloring,but also had new bands compared with parent.Peroxidase zymogram of hybrid F1 mainly displayed incomplete complementary and hybrid type of parent.[Conclusion]Protein spectrum and peroxidase zymogram could be used as the biochemical markers for the identification of hybrids of lily,which could also detect the target traits of plant.