The extracts from hulls, brans and flours of Fagopyrum esculentum M6ench （FEM, three varieties） and Fagopyrum tartaricum L. Gaerth （FTG, seven varieties） were screened for free and bound phenolic content or total ...The extracts from hulls, brans and flours of Fagopyrum esculentum M6ench （FEM, three varieties） and Fagopyrum tartaricum L. Gaerth （FTG, seven varieties） were screened for free and bound phenolic content or total phenolic content （TPC）, as well as 1,1 diphenyl-2-picryl hydrazyl （DPPH） free radical scavenging activity and reducing power. Free phenolics were predominant in buckwheat hulls, brans and flours. FEM hulls extract exhibited the highest reducing power and DPPH free radical scavenging activity with the average ECs0 84.54 μg mL^-1 and IC50 11.54 μg mL^-1 respectively, FTG brans extract had the highest average TPC （24.87 mg GAE g^-1 DW）, and FEM flours extract showed the lowest TPC, reducing power and radical scavenging activity. Furthermore, the correlations among TPC, DPPH free radical scavenging activity and reducing power of all the samples were investigated. The rank correlation coefficient （rs） between reducing power and DPPH free radical scavenging activity of buckwheat hulls, between TPC and DPPH free radical scavenging activity of buckwheat flours were 0.76 and 0.79, respectively （P〈0.05）. However, there is no significant correlation between the remaining indexes of hulls and flours, as well as the ten buckwheat brans. This result indicated that some non-phenolic compounds also contributed to the total antioxidant activity in hulls, brans and flours of buckwheats. This study demonstrated that buckwheat hulls and brans, rather than flours, are good source of antioxidants.展开更多
Ningqiao 1 was selected as the material to study the effects of planting density on physiological indices,agronomic traits and yield of buckwheat.The results showed that high density resulted in decreases of chlorophy...Ningqiao 1 was selected as the material to study the effects of planting density on physiological indices,agronomic traits and yield of buckwheat.The results showed that high density resulted in decreases of chlorophyll content,soluble protein content and activity of SOD,POD and CAT,and acceleration of MDA accumulation under drought conditions.Low density could effectively improve the grain number per plant,grain weight per plant,1000-grain weight and yield in drought conditions.展开更多
为丰富普通荞麦（Fagopyrum esculentum Moench）的序列信息，挖掘有效的 SSR 标记，基于Hiseq 2000测序平台对普通荞麦的种子转录谱进行测序、分析，利用 Misa 软件进行 SSR 位点扫描，采用Primer 5．0引物设计程序对其中的300个 SSR ...为丰富普通荞麦（Fagopyrum esculentum Moench）的序列信息，挖掘有效的 SSR 标记，基于Hiseq 2000测序平台对普通荞麦的种子转录谱进行测序、分析，利用 Misa 软件进行 SSR 位点扫描，采用Primer 5．0引物设计程序对其中的300个 SSR 位点设计引物，随机挑选40对引物对19个普通荞麦品系进行遗传多样性分析。结果表明：测序共产生20508824条高质量的短序列（read），总长度为1889111004 bp，通过拼接最终获得54947条转录本（transcript）和36133个独立基因（unigene）。在2226个独立基因中发现了2666个 SSR 位点。有20对引物（占50％）能扩增出目标产物，其中，12对有多态性，多态性信息量（PIC）范围为0．10～0．93，平均为0．57，多态性程度高。展开更多
基金supported by the Fundamental Research Funds for the Central Universities,China (XDJK2012B014)
文摘The extracts from hulls, brans and flours of Fagopyrum esculentum M6ench （FEM, three varieties） and Fagopyrum tartaricum L. Gaerth （FTG, seven varieties） were screened for free and bound phenolic content or total phenolic content （TPC）, as well as 1,1 diphenyl-2-picryl hydrazyl （DPPH） free radical scavenging activity and reducing power. Free phenolics were predominant in buckwheat hulls, brans and flours. FEM hulls extract exhibited the highest reducing power and DPPH free radical scavenging activity with the average ECs0 84.54 μg mL^-1 and IC50 11.54 μg mL^-1 respectively, FTG brans extract had the highest average TPC （24.87 mg GAE g^-1 DW）, and FEM flours extract showed the lowest TPC, reducing power and radical scavenging activity. Furthermore, the correlations among TPC, DPPH free radical scavenging activity and reducing power of all the samples were investigated. The rank correlation coefficient （rs） between reducing power and DPPH free radical scavenging activity of buckwheat hulls, between TPC and DPPH free radical scavenging activity of buckwheat flours were 0.76 and 0.79, respectively （P〈0.05）. However, there is no significant correlation between the remaining indexes of hulls and flours, as well as the ten buckwheat brans. This result indicated that some non-phenolic compounds also contributed to the total antioxidant activity in hulls, brans and flours of buckwheats. This study demonstrated that buckwheat hulls and brans, rather than flours, are good source of antioxidants.
基金Ningxia Natural Science Foundation(NZ15270)National Agriculture Research System of Oat and Buckwheat(CARS-08-E-5).
文摘Ningqiao 1 was selected as the material to study the effects of planting density on physiological indices,agronomic traits and yield of buckwheat.The results showed that high density resulted in decreases of chlorophyll content,soluble protein content and activity of SOD,POD and CAT,and acceleration of MDA accumulation under drought conditions.Low density could effectively improve the grain number per plant,grain weight per plant,1000-grain weight and yield in drought conditions.
文摘为丰富普通荞麦（Fagopyrum esculentum Moench）的序列信息，挖掘有效的 SSR 标记，基于Hiseq 2000测序平台对普通荞麦的种子转录谱进行测序、分析，利用 Misa 软件进行 SSR 位点扫描，采用Primer 5．0引物设计程序对其中的300个 SSR 位点设计引物，随机挑选40对引物对19个普通荞麦品系进行遗传多样性分析。结果表明：测序共产生20508824条高质量的短序列（read），总长度为1889111004 bp，通过拼接最终获得54947条转录本（transcript）和36133个独立基因（unigene）。在2226个独立基因中发现了2666个 SSR 位点。有20对引物（占50％）能扩增出目标产物，其中，12对有多态性，多态性信息量（PIC）范围为0．10～0．93，平均为0．57，多态性程度高。
