To screen species-specific DNA probes for identification of Fallopia muhiflora, the genomic DNA （gDNA） suppression subtraction hybridization （SSH） between F. muhiflora and F. muhiflora var. ciliinervis was firstly...To screen species-specific DNA probes for identification of Fallopia muhiflora, the genomic DNA （gDNA） suppression subtraction hybridization （SSH） between F. muhiflora and F. muhiflora var. ciliinervis was firstly performed. The obtained differential gDNA fragments by SSH were then hybridized with gDNA ar- rays consisting of multiple whole genomes of several species （adulterants and/or closely related species of F. muhiflora） and four differential fragments were screened uniquely representing F. muhiflora, which could be used as F. muhiflora species-specific probes. The screened DNA probes were tested by reverse dot blot hybridization and the results demonstrated that these probes could be used reliably to identify F, muhiflora. The species-specific DNA probes obtained in this study exhibited broad application prospects in the preparation of gene chips for identifying Chinese traditional medicines and the authentication of germplasm re- sources and crude drugs of F. muhiflora.展开更多
Considering the high abundance of knotweeds along river courses, the expected increase of invasion and the consequent negative impacts on riparian ecosystems, there is a high demand for innovative approaches and manag...Considering the high abundance of knotweeds along river courses, the expected increase of invasion and the consequent negative impacts on riparian ecosystems, there is a high demand for innovative approaches and management strategies. While a primary aim of weed management is to reduce the population of an invasive plant species, the goal of the pre-sented nature-based engineering solution (NABES) is to reinstall native riparian forests and to restore ecosystem functioning. The concept of NABES is to support the implemented species by frequent removal of the knotweed shoots until the native vegetation represses the knotweeds by root competition and shadow pressure. In order to be able to develop adaptive knotweed management strategies, knowledge concerning sea-sonal biomass development and the most effective maintenance intervals must be improved. Additionally, greater understanding of the interaction between invasive and native species is essential. In the present study, the effectiveness of a willow brush mattress (a frequent technique for controlling riverbank erosion) in combination with adapted management strategies was tested on a Fallopia japonica-dominated brook embankment. Due to its high ecological amplitude and excellent soil bioengineering properties the species S. purpurea was used. In the upper part of the embankment, F. japonica shoot production was by far the strongest, while it was low in the sections next to the water. The strongest biomass production was observed in the months April and May. Even though the temporal interval between shoot removal was increased, shoot production decreased strongly and nearly ceased in August. Branches of S. purpurea with contact to the water of the brook showed good development. In contrast to F. japonica, which suffered a rapid decrease in biomass production after the third survey, the coverage ratio of S. purpurea decreased gradually over the vegetation period.展开更多
In order to develop an efficient identification method for Fallopia plants and drugs,molecular analysis of the partial matK gene sequences was performed on 6 Fallopia species.Based on the matK sequences,a phylogenetic...In order to develop an efficient identification method for Fallopia plants and drugs,molecular analysis of the partial matK gene sequences was performed on 6 Fallopia species.Based on the matK sequences,a phylogenetic tree was constructed,which showed that different populations of inter-and intra-species could be specified and distinguished.The matK gene sequences of the 6 Fallopia species were all found to be of 1 271 bp in length,with some nucleotide variations throughout the entire sequences.The nucleotide difference at position 1 041 could distinguish F.denticulata from others,while specific nucleotide at position 1 154 became identification markers for F.aubertii.Moreover,four specific marker sites for F.multiflora var.ciliinerve at positions 216,224,1 060 and 1 179,seven for F.convolvulus at 318,765,772,874,936,952 and 1 036,and seven for F.dentate alata at 111,192,366,450,457,1 032 and 1 074 were also observed.By detecting the marker nucleotides and analyzing the phylogenetic relationship,the botanical origins of five inspected drugs were determined,suggesting that matK sequences can be used for authenticating Fallopia plants and drugs.展开更多
基金Supported by Fund of Guangdong Provincial Administration of Traditional Chinese Medicine(20111251)
文摘To screen species-specific DNA probes for identification of Fallopia muhiflora, the genomic DNA （gDNA） suppression subtraction hybridization （SSH） between F. muhiflora and F. muhiflora var. ciliinervis was firstly performed. The obtained differential gDNA fragments by SSH were then hybridized with gDNA ar- rays consisting of multiple whole genomes of several species （adulterants and/or closely related species of F. muhiflora） and four differential fragments were screened uniquely representing F. muhiflora, which could be used as F. muhiflora species-specific probes. The screened DNA probes were tested by reverse dot blot hybridization and the results demonstrated that these probes could be used reliably to identify F, muhiflora. The species-specific DNA probes obtained in this study exhibited broad application prospects in the preparation of gene chips for identifying Chinese traditional medicines and the authentication of germplasm re- sources and crude drugs of F. muhiflora.
文摘Considering the high abundance of knotweeds along river courses, the expected increase of invasion and the consequent negative impacts on riparian ecosystems, there is a high demand for innovative approaches and management strategies. While a primary aim of weed management is to reduce the population of an invasive plant species, the goal of the pre-sented nature-based engineering solution (NABES) is to reinstall native riparian forests and to restore ecosystem functioning. The concept of NABES is to support the implemented species by frequent removal of the knotweed shoots until the native vegetation represses the knotweeds by root competition and shadow pressure. In order to be able to develop adaptive knotweed management strategies, knowledge concerning sea-sonal biomass development and the most effective maintenance intervals must be improved. Additionally, greater understanding of the interaction between invasive and native species is essential. In the present study, the effectiveness of a willow brush mattress (a frequent technique for controlling riverbank erosion) in combination with adapted management strategies was tested on a Fallopia japonica-dominated brook embankment. Due to its high ecological amplitude and excellent soil bioengineering properties the species S. purpurea was used. In the upper part of the embankment, F. japonica shoot production was by far the strongest, while it was low in the sections next to the water. The strongest biomass production was observed in the months April and May. Even though the temporal interval between shoot removal was increased, shoot production decreased strongly and nearly ceased in August. Branches of S. purpurea with contact to the water of the brook showed good development. In contrast to F. japonica, which suffered a rapid decrease in biomass production after the third survey, the coverage ratio of S. purpurea decreased gradually over the vegetation period.
基金Supported by the Natural Science Foundation of Guangdong Province(06019716)
文摘In order to develop an efficient identification method for Fallopia plants and drugs,molecular analysis of the partial matK gene sequences was performed on 6 Fallopia species.Based on the matK sequences,a phylogenetic tree was constructed,which showed that different populations of inter-and intra-species could be specified and distinguished.The matK gene sequences of the 6 Fallopia species were all found to be of 1 271 bp in length,with some nucleotide variations throughout the entire sequences.The nucleotide difference at position 1 041 could distinguish F.denticulata from others,while specific nucleotide at position 1 154 became identification markers for F.aubertii.Moreover,four specific marker sites for F.multiflora var.ciliinerve at positions 216,224,1 060 and 1 179,seven for F.convolvulus at 318,765,772,874,936,952 and 1 036,and seven for F.dentate alata at 111,192,366,450,457,1 032 and 1 074 were also observed.By detecting the marker nucleotides and analyzing the phylogenetic relationship,the botanical origins of five inspected drugs were determined,suggesting that matK sequences can be used for authenticating Fallopia plants and drugs.
