On the morning on December 30,2015,the 22nd Session of the Standing Committee of the Twelfth People＇s Congress of Guangdong Province voted through Regulations of Guangdong Province on Population and Family Planning（...On the morning on December 30,2015,the 22nd Session of the Standing Committee of the Twelfth People＇s Congress of Guangdong Province voted through Regulations of Guangdong Province on Population and Family Planning（Draft Revision）（hereinafter referred to as the New Regulaions）.展开更多
On December 27,2015,with157 votes in favor and 2abstentions,the 18th Session of the Standing Committee of the Twelfth National People＇s Congress adopted the Decision of the Standing Committee of the National People＇...On December 27,2015,with157 votes in favor and 2abstentions,the 18th Session of the Standing Committee of the Twelfth National People＇s Congress adopted the Decision of the Standing Committee of the National People＇s Congress on Amending Population and Family Planning Law.展开更多
On January 15,2016,National Health and Family Planning Commission（NHFPC）convened a regular press briefing.Directr-General Mao Qun’an introduced the general requirements for health and family planning work in2016,in...On January 15,2016,National Health and Family Planning Commission（NHFPC）convened a regular press briefing.Directr-General Mao Qun’an introduced the general requirements for health and family planning work in2016,including fully implementing the spirit of the 18th National Congress of the Communist Party of China（CPC）,the Third,Fourth and Fifth Plenary Sessions of the 18th CPC CentralCommittee, and a series of important speeches by General Secretary Xi Jinping.展开更多
Piericidin A1,a member ofɑ-pyridone antibiotic,exhibits various biological activities such as antimicrobial,antifungal,and antitumor properties and possesses potent respiration-inhibitory activity against insects due...Piericidin A1,a member ofɑ-pyridone antibiotic,exhibits various biological activities such as antimicrobial,antifungal,and antitumor properties and possesses potent respiration-inhibitory activity against insects due to its competitive binding capacity to mitochondrial complex I.The biosynthetic pathway of piericidin A1 has been reported in Streptomyces piomogeues var.Hangzhouwanensis,while the regulatory mechanism remains poorly understood.In this study,a Streptomyces antibiotic regulatory protein(SARP)family transcriptional regulator PieR was characterized.Genetic disruption and complementation manipulations revealed that PieR positively regulated the production of piericidin A1.Moreover,the overexpression of pieR contributed to the improvement of piericidin A1 productivity.The real-time quantitative PCR(RT-qPCR)was carried out and the data showed that pieR stimulated the transcription of all the biosynthesis-related genes for piericidin A1.In order to explore the regulatory mechanism,electrophoresis mobility shift assays(EMSA)and DNase I footprinting experiments have been conducted.A protected region covering 50 nucleotides within the upstream region of pieR was identified and two 5-nt direct repeat sequences(5′-CCGGA-3′)in the protected region were found.These findings,taken together,set stage for transcriptional control engineering in the view of optimizing piericidin A1 production and thus provide a viable potent route for the construction of strains with high productivity.展开更多
文摘On the morning on December 30,2015,the 22nd Session of the Standing Committee of the Twelfth People＇s Congress of Guangdong Province voted through Regulations of Guangdong Province on Population and Family Planning（Draft Revision）（hereinafter referred to as the New Regulaions）.
文摘On December 27,2015,with157 votes in favor and 2abstentions,the 18th Session of the Standing Committee of the Twelfth National People＇s Congress adopted the Decision of the Standing Committee of the National People＇s Congress on Amending Population and Family Planning Law.
文摘On January 15,2016,National Health and Family Planning Commission（NHFPC）convened a regular press briefing.Directr-General Mao Qun’an introduced the general requirements for health and family planning work in2016,including fully implementing the spirit of the 18th National Congress of the Communist Party of China（CPC）,the Third,Fourth and Fifth Plenary Sessions of the 18th CPC CentralCommittee, and a series of important speeches by General Secretary Xi Jinping.
基金the National Natural Science Foundation of China(31630002,31700029,31770038,31470183,21661140002 and 31170085)the Ministry of Science and Technology+1 种基金Shanghai Pujiang Program from the Shanghai Municipal Council of Science and Technology(12PJD021)China Postdoctoral Science Foundation(2017M620151).
文摘Piericidin A1,a member ofɑ-pyridone antibiotic,exhibits various biological activities such as antimicrobial,antifungal,and antitumor properties and possesses potent respiration-inhibitory activity against insects due to its competitive binding capacity to mitochondrial complex I.The biosynthetic pathway of piericidin A1 has been reported in Streptomyces piomogeues var.Hangzhouwanensis,while the regulatory mechanism remains poorly understood.In this study,a Streptomyces antibiotic regulatory protein(SARP)family transcriptional regulator PieR was characterized.Genetic disruption and complementation manipulations revealed that PieR positively regulated the production of piericidin A1.Moreover,the overexpression of pieR contributed to the improvement of piericidin A1 productivity.The real-time quantitative PCR(RT-qPCR)was carried out and the data showed that pieR stimulated the transcription of all the biosynthesis-related genes for piericidin A1.In order to explore the regulatory mechanism,electrophoresis mobility shift assays(EMSA)and DNase I footprinting experiments have been conducted.A protected region covering 50 nucleotides within the upstream region of pieR was identified and two 5-nt direct repeat sequences(5′-CCGGA-3′)in the protected region were found.These findings,taken together,set stage for transcriptional control engineering in the view of optimizing piericidin A1 production and thus provide a viable potent route for the construction of strains with high productivity.
