利用CRISPR/Cas9技术构建FARS2基因定点突变的大鼠模型

目的:利用CRISPR/Cas9技术构建FARS2基因特定位点突变的大鼠模型。方法:根据FARS2基因序列,设计FARS2基因特异性的单链向导RNA(sgRNA)引物序列并克隆入pU57-T7-GDNA载体。利用T7RNA聚合酶体外转录sgRNA和Cas9mRNA。将体外转录的sgRNA/Cas9mRNA显微注射入SD大鼠受精卵,通过PCR和基因测序对FARS2基因特定位点突变进行检测和鉴定。繁育FARS2基因敲除大鼠并分析后代突变情况。结果:基因测序证实成功构建表达sgRNA载体,成功将sgRNA和Cas9mRNA直接注射入大鼠受精卵。基因测序鉴定获得5只F0代初建鼠。DNA测序结果证实5号大鼠(ID#5)发生了gac>tac突变,该突变并可遗传至子代大鼠。结论:利用CRISPR/Cas9技术成功制备FARS2基因定点突变的大鼠模型,为进一步研究FARS2的功能奠定了基础。

Sedimentary Environments and Correlative Sequence Stratigraphy of Upper Cretaceous—Paleogene Succession in Shiraz Area, Fars, SW IRAN

Sequence stratigraphic evidences have been used in this research to investigate the stratigraphic correlation of cretaceous paleogene succession in Shiraz area, Fars, SW Iran. For this purpose, 4 stratigraphic sections have been chosen and provided. According to petrographic study and field evidences, 4 depositional sequences were identified including the Tarbur, Sachun and Jahrum formations in the east and the Gurpi, Pabadeh and Asmari formations in the west of study area. After identifying the boundaries and systems tract facies, it has been found that the mentioned formations have been deposited among the continuous/discontinuous boundaries in an equivalent manner. In this case, the purple shale (between the Gurpi and Pabdeh formations) could be considered equivalent to the upper part of the Sachun formation. From the point of Paleogeography, the plat-form sedimentary environment covered almost the whole area during the Oligo-Miocene age, so that the Asmari formation would have been deposited, which is marked by sharp contact as disconformity on Jahrum formation in the east of the basin and with continuous contact (C.C) on the Pabdeh formation in the west and southwest.

Does prospective permutation scan statistics work well with cutaneous leishmaniais as a high-frequency or malaria as a low-frequency infection in Fars province, Iran?

Objective: To determine whether permutation scan statistics was more efficient in finding prospective spatial-temporal outbreaks for cutaneous leishmaniasis(CL) or for malaria in Fars province, Iran in 2016. Methods: Using time-series data including 29 177 CL cases recorded during 2010-2015 and 357 malaria cases recorded during 2010-2015, CL and malaria cases were predicted in 2016. Predicted cases were used to verify if they followed uniform distribution over time and space using space-time analysis. To testify the uniformity of distributions, permutation scan statistics was applied prospectively to detect statistically significant and non-significant outbreaks. Finally, the findings were compared to determine whether permutation scan statistics worked better for CL or for malaria in the area. Prospective permutation scan modeling was performed using SatScan software. Results: A total of 5 359 CL and 23 malaria cases were predicted in 2016 using time-series models. Applied timeseries models were well-fitted regarding auto correlation function, partial auto correlation function sample/model, and residual analysis criteria(Pv was set to 0.1). The results indicated two significant prospective spatial-temporal outbreaks for CL(P<0.5) including Most Likely Clusters, and one non-significant outbreak for malaria(P>0.5) in the area. Conclusions: Both CL and malaria follow a space-time trend in the area, but prospective permutation scan modeling works better for detecting CL spatial-temporal outbreaks. It is not far away from expectation since clusters are defined as accumulation of cases in specified times and places. Although this method seems to work better with finding the outbreaks of a high-frequency disease; i.e., CL, it is able to find non-significant outbreaks. This is clinically important for both high-and low-frequency infections; i.e., CL and malaria.

Determining endemic values of cutaneous leishmaniasis in Iranian Fars province by retrospectively detected clusters and receiver operating characteristic curve analysis

Objective: To determine the endemic values of cutaneous leishmaniasis in different cities of Fars province, Iran. Methods: Totally, 29 201 cases registered from 2010 to 2015 in Iranian Fars province were selected, and the endemic values of cutaneous leishmaniasis were determined by retrospective clusters derived from spatiotemporal permutation modeling on a time-series design. The accuracy of the values was assessed using receiver operating characteristic(ROC) curve. SPSS version 22, Arc GIS, and ITSM 2002 software tools were used for analysis. Results: Nine statistically significant retrospective clusters(P<0.05) resulted in finding seven significant and accurate endemic values(P<0.1). These valid endemic scores were generalized to the other 18 cities based on 6 different climates in the province. Conclusions: Retrospectively detected clusters with the help of ROC curve analysis could help determine cutaneous leishmaniasis endemic values which are essential for future prediction and prevention policies in the area.

Application of Statistical Methods to Biomarkers for Geochemical Evaluation and Genetic Type Determination of Gas Condensates in the Persian Gulf and Coastal Fars,Southern Iran

In a comprehensive geochemical study,the genetic relationships among 14 samples of gas condensates from the Persian Gulf were investigated for the purpose of evaluating the respective source rocks in terms of both age and sedimentary paleoenvironment.Chemometric analysis was used for categorization and determination of a certitude range to determine the genetic type of the condensate families in the studied basin.The samples were collected from Late Permian-Triassic reservoirs(Dalan and Kangan formations)located in 6 gasfields(gas condensate)hosting some of Iran’s most important gas/gas condensate reserves.Obtained by gas chromatography-mass spectrometry(GC-MS),a total of 16 biomarker parameters(10 maturity-related parameters and 6 sedimentary environment-related parameters)were used to evaluate the samples in terms of thermal maturity(and hence their positions in the maturity chart),the sedimentary environment of the source rock and the lithology.Application of Hierarchical Clustering Analysis(HCA)and Principal Component Analysis(PCA)to the collected data led to the categorization of the samples into three main genetic groups,Ⅰ-Ⅲ.GroupsⅠandⅢwere found to be located in the east and the west of the Persian Gulf,respectively,while GroupⅡwas developed between the two other groups.

Evaluation of Hydrocarbon Potential in the Jahrom Area, Interior Fars, Zagros

The study area is located in the Fars area (Interior Fars sub-basin). This area, which is part of the Zagros fold-thrust belt, is located in Zagros simple folded sub zone. It is known as one of the most important regions in the world Gas rich. In this area, anticlinal folds are a different orientation of such as the North Western-South Eastern, East-West and North Eastern-South Western. The Karbasi and Khaftar anticlines are case study anticlines in the interior Fars sub-basin (Fassa area). The anticlines in the Fars region, which are located in the Zagros fold-thrust belt, are valuable because they possess several hydrocarbons. The main aim of this paper is evaluation of hydrocarbon potential in the Jahrom area (Interior Fars) based on interpretation of the Isopach maps and well data. This paper presents part of the results of a regional study of the Coastal Fars area in the Zagros Simply folded belt, based on original fieldwork, satellite images, structural sections, geological maps and well data. In order to investigate of periods Nezamabad fault activity, Isopach maps of the study area has been prepared based on well drilling data and the thickness of the drilled formations. In this study utilizes the thickness of the drilled formations in the study area, for each sedimentary unit was carried out to prepare Isopach maps. The Isopach map prepared based on GIS software method and exploration well data. The Nezamabad fault has been lacked of activity at the deposition time of the Dalan, Kangan, Dashtak, Fahliyan, Gadvan, Dariyan, Kazhdomi, Sarvak, Gurpi and Pabdeh formations within the Khaftar and Karbasi anticline area. Accordingly, changes in basin floor, at the time of sedimentation these formations has not been affected by the Nezamabad fault. Probably the Nezamabad fault has been activated during deposition of the Surmeh, Hith, Laffan and Ilam formations and in this area paleo-basin has been controlled by this fault. In other words, it seems that, activity of the Nezamabad fault in this area have been limited to Jurassic and Cretaceous periods (Coniacian and Senomanian). This can means that in this periods time, the Nezamabad fault have been active sedimentation along with tectonic processes and activities affected on thickness of the Surmeh, Hith, Laffan and Ilam formations. Finally, it seems that the Nezamabad Fault activity has been affected on the source rock, reservoir rock and cap rock and then controlled distributing of hydrocarbon System of the study area in the Khaftar and Karbasi as anticlinal oil traps.

指纹自动识别系统(FARS)简介

指纹自动识别系统(FARS)是公安部重点指令性课题,由公安部第二研究所承担并完成。FARS为自动与半自动(MFRS)相结合的系统,采用公安部第二研究所独创的指纹'特征图'识别方法。'特征图'以指纹细节特征作为原始数据,系统通过摄像。

基于全基因组水平的花生FAR1转录因子家族分析

FAR1是一类起源于转座子酶的转录因子,在光敏色素A(phyA)信号通路的启动中起到非常重要的作用。基于全基因组水平对其家族分析,可为深入研究FAR1基因在花生生长发育、逆境胁迫响应中的分子调控机理提供依据。本研究在花生基因组中共鉴定出60个FAR1基因,该转录因子家族成员分成3个亚组,Ⅰ、Ⅱ、Ⅲ亚组中分别包含了21、20和19个花生FAR1蛋白。65%直系同源基因的Ka/Ks值小于0.5,这些基因结构稳定、较为保守,经历了较强的负选择作用。转录组数据分析发现,大部分FAR1基因在花生叶片中表达。本研究结果有助于了解花生FAR1基因家族的进化与功能,为下一步深入研究该基因家族的功能提供依据。

一例FARS2基因变异所致联合氧化磷酸化缺陷14型患儿的遗传学分析

目的:探讨一例表现为无诱因惊厥发作、生长发育迟缓、血乳酸值升高的2月龄患儿的遗传学病因。方法:应用二代测序技术对患儿进行全外显子组和线粒体环基因测序,用Sanger测序对候选致病变异进行验证,依据美国医学遗传学及基因组学学会相关指南进行致病性分析。检索PubMed、万方数据库和中国知网数据库中FARS2变异所致的联合氧化磷酸化缺乏症14型(COXPD14)的病例报道,总结其临床特征及基因变异谱。结果:全外显子组测序提示患儿携带FARS2基因c.925G>A(p.G309S)和c.405C>A(p.H135Q)复合杂合变异;Sanger测序证实这两个变异分别遗传自母亲和父亲。其中c.925G>A为HGMD数据库已收录的致病变异,c.405C>A预测为疑似致病变异。检索文献另发现30例COXPD14患者,其FARS2变异类型包括错义变异、剪接变异、读码框内碱基缺失和编码区微缺失。结论:COXPD14临床表现以发育迟缓(96%)、癫痫发作(97%)和乳酸升高(96%)最为经典。FARS2基因c.925G>A(p.G309S)和c.405C>A(p.H135Q)复合杂合变异是本例患儿的发病原因。

运用FARS模型对语篇修辞结构的实证分析

本文简要介绍"语篇关系结构分析框架理论"(the Framework for the Analysis of the Relational Structure of Texts,FARS)的7种修辞结构关系类型,并运用该模型对2段语篇进行分析。比较了两段语篇修辞关系运用的特点,发现语篇一修辞手法运用灵活多样;而语篇二中部分修辞关系重复出现,过度使用某些直观形式标记。

Background removal from global auroral images:Data-driven dayglow modeling

Global images of auroras obtained by cameras on spacecraft are a key tool for studying the near-Earth environment.However,the cameras are sensitive not only to auroral emissions produced by precipitating particles,but also to dayglow emissions produced by photoelectrons induced by sunlight.Nightglow emissions and scattered sunlight can contribute to the background signal.To fully utilize such images in space science,background contamination must be removed to isolate the auroral signal.Here we outline a data-driven approach to modeling the background intensity in multiple images by formulating linear inverse problems based on B-splines and spherical harmonics.The approach is robust,flexible,and iteratively deselects outliers,such as auroral emissions.The final model is smooth across the terminator and accounts for slow temporal variations and large-scale asymmetries in the dayglow.We demonstrate the model by using the three far ultraviolet cameras on the Imager for Magnetopause-to-Aurora Global Exploration(IMAGE)mission.The method can be applied to historical missions and is relevant for upcoming missions,such as the Solar wind Magnetosphere Ionosphere Link Explorer(SMILE)mission.

FAR3d程序对EAST上阿尔芬不稳定性识别过程中的应用

为更好理解EAST实验中的等离子体不稳定性现象,文章从实验和模拟两个角度,对EAST中高βN放电区间的阿尔芬不稳定性现象进行了分析。在实验上,本文使用高频磁探针和电子回旋辐射诊断对不稳定性的频率、位置、环向模数进行判断。在模拟上首次应用了FAR3d程序,识别了在ρ=0.45、频率为87 kHz的主导模式TAE(toroidicity-induced Alfven eigenmode),以及ρ=0.55、频率为62 kHz的次主导模式EPM(energetic particle mode),环向模数均为2,与实验中的测量结果对比在误差范围内基本一致,自洽地识别了不稳定性的类型并且得到了位置与频率信息。在此基础上,对有限拉莫轨道(finite Larmor radius, FLR)效应进行评估。模拟结果显示,在EAST高βN放电区间,FLR效应对识别低环向模数n的不稳定性模式的影响较小。在该放电的模式的识别过程中可关闭FLR以加速计算。FAR3d程序高效的计算能力和精准的模拟结果,可为后续EAST的实验提供指导。

术前外周血SII、NLR、PLR、FAR、MLR及CA125在卵巢透明细胞癌诊断中的临床价值分析

目的:探讨术前外周血系统性免疫炎症指数(SII)、中性粒细胞–淋巴细胞比值(NLR)、血小板与淋巴细胞比值(PLR)、纤维蛋白原–白蛋白比值(FAR)、单核细胞–淋巴细胞比值(MLR)及血清糖类抗原125(CA125)在卵巢良性肿瘤及卵巢透明细胞癌(OCCC)中的差异,分析其诊断价值。方法:选取青岛大学附属医院2014年1月~2023年9月收治的114例卵巢透明细胞癌(卵巢透明细胞癌组)和119例卵巢良性肿瘤(卵巢良性肿瘤组)患者的临床资料进行回顾性分析。检测术前外周血中SII、NLR、PLR、FAR、MLR及CA125水平,对每组病例所对应的SII、NLR、PLR、FAR、MLR及CA125进行对比分析,运用受试者工作特征(ROC)曲线分析SII、NLR、PLR、FAR、MLR及CA125在卵巢透明细胞癌中诊断价值。结果:OCCC组术前外周血SII、NLR、PLR、MLR及CA125水平高于卵巢良性肿瘤组,FAR水平低于卵巢良性肿瘤组,差异有统计学意义。SII、NLR、PLR、FAR、MLR及CA125的ROC曲线下面积(AUC)分别为0.729、0.720、0.708、0.869、0.719、0.696。结论:术前外周血SII、NLR、PLR、FAR、MLR可作为辅助临床评估与诊断OCCC的指标。

纤维蛋白原与白蛋白比值在妇科恶性肿瘤中的研究进展

妇科恶性肿瘤的发病率高,病因复杂,复发率也较高,对患者的身体和心理均造成严重影响。如何及时准确的评估疾病的预后,给予针对性的干预措施显得尤为重要。近年来,许多研究均表明炎症、营养状况与癌症的预后有关。纤维蛋白原与白蛋白比值( fibrinogen to albumin ratio,FAR)较单一指标能更好的反映患者的炎症和营养状态,被用于多种疾病的预后评估。FAR是新发现的一种炎症指标,检测便利,成本低廉,可以为临床预后判断提供更多的参考。本文就FAR在妇科恶性肿瘤领域的研究进展予以综述。

Analysis and identification of the hidden relationships between effective factors in the mortality rate caused by road accidents: A case study of Fars Province, Iran

Purpose: An analysis and identification of the hidden relationships between effective factors in the mortality rate caused by road accidents in Fars Province of Iran to prevent and reduce traffic accidents in the future. Methods: This cross-sectional study was conducted to integrate all the pervious researches performed on mortality rate of road traffic accidents in Fars Province from March 21,2013 to March 20,2017. In order to reveal the relationships between the factors affecting mortality rates of road traffic accidents, the data regarding road traffic accidents extracted from resources such as Legal Medicine Organization, Traffic Police, Accident & Emergency Department, as well as Department of Roads and Urban Development of Fars Province, then cleaned and the applicable attributes embedded in the data all aggregated for further analysis. It should be noted that the data not related to Fars Province were deleted, the data analyzed, converted and the aggregation between various attributes identified. The aggregation between these different attributes as well as the FP-growth algorithm and two indexes of support and confidence calculated and interesting and effective rules extracted. In the end, several accident-p「ovoking factors, the degree of consecutive and interdepende nee of each one in road accide nts identified and in traduced. The statistical analysis was conducted by using Rapid Miner software. Results: Of the 6216 people dead due to road traffic accidents, 4865 (79.02%) were male and 1292 (20.98%) were female, 59 of them have no clear gender. The largest portion of people died of road traffic accidents belonged to married and self-employed men who collided with motorcycles in autumn. Moreover, young individuals (aged 19-40 years) w让h secondary educational level who died of accidents in summer at 12:00 a.m. and then 5:00 p.m. in outer city main roads of Kazerun-Shiraz, then Darab- Shiraz, Fasa-Darab and in within-city main streets had the highest mortality rates. Among women, the middle-aged group (aged 41-65 years) followed by young-aged group (aged 19-40 years) with elementary educational level and then illiterate accounted for the highest mortality rate of road traffic accidents. The automobiles involved in accidents included Pride, Peugeot 405, Peykan pickup, Sama nd, Peugeot Pars, other vehicles and motorcycles. Conclusion: The high mortality rate of illiterate and low-literate in various age groups indicates that educational level plays a crucial role as a factor in road accidents, requiring related organizations such as Traffic Police and Ministry of Education to take necessary measures and policies.

财务会计报告系统（FARS）专题

用户日常操作需要用到的菜单包括环境维护、用户管理、报表管理、综合查询、日志查看、密码修改等以及上述选项的子菜单。这些菜单选项有的只能由用户管理员或一般操作员使用。下面对用户常用的菜单使用作逐一说明，对于省分行专用菜单不作介绍。

机翻字幕质量评估的模型构建研究——以“人人译视界”和“讯飞听见字幕”为例

AI时代神经网络翻译技术的飞速发展催生了一批视频译制平台。以“人人译视界”和“讯飞听见字幕”为研究对象,以动画作品《中华勤学故事》机翻字幕为研究素材,探究国内视频译制平台机翻字幕质量。基于FAR语际字幕评估模型提出了机翻字幕评估模型(FAR 2.0),从功能对等、接受程度、阅读体验三方面对机翻字幕的语义错误、术语翻译错误、译文冗余等问题进行了深入分析。根据研究结果,提出增强语音数据标注积累、加大机器翻译语料训练及强化翻译引擎的意群切分等三点优化建议。

Research Progress in FDA’s Focus Areas of Regulatory Science for Drugs and Suggestions for China

Objective To sort out the focus areas of FDA’s scientific supervision and to achieve corresponding results,so as to provide some suggestions for the scientific research and innovation of drug supervision in China.Methods In-depth research was carried out in the fields of safety evaluation tools,clinical trial innovation,new methods of product quality evaluation,information science and so on.Results and Conclusion The research foundation of drug supervision related technology in China is weak,and a systematic supervision system has not been formed.Learning from the scientific experience of drug supervision in the United States,we can promote the development of the focus areas of drug supervision in China.Besides,we should improve the standard management ability while perfecting the regulations and standards system.Lastly,we should establish a system for government and industry-university-research to realize the full-cycle supervision of drugs.

Microsphere femtosecond laser sub-50 nm structuring in far field via non-linear absorption

Creation of arbitrary features with high resolution is critically important in the fabrication of nano-optoelectronic devices.Here,sub-50 nm surface structuring is achieved directly on Sb2S3 thin films via microsphere femtosecond laser irradi-ation in far field.By varying laser fluence and scanning speed,nano-feature sizes can be flexibly tuned.Such small patterns are attributed to the co-effect of microsphere focusing,two-photons absorption,top threshold effect,and high-repetition-rate femtosecond laser-induced incubation effect.The minimum feature size can be reduced down to~30 nm(λ/26)by manipulating film thickness.The fitting analysis between the ablation width and depth predicts that the feature size can be down to~15 nm at the film thickness of~10 nm.A nano-grating is fabricated,which demonstrates desirable beam diffraction performance.This nano-scale resolution would be highly attractive for next-generation laser nano-lithography in far field and in ambient air.

影片《百鸟朝凤》的英译字幕质量评估研究

目前国内对于影视字幕译本质量的评估研究并不多。《百鸟朝凤》是一部民俗电影,影片涵盖了大量中国元素,本文采用瑞典学者Jan Pederson提出的FAR字幕翻译评估模型为基本理论框架,在这一评估模式下,发现本部影片字幕共存在21个错误,在分析错误出现的原因之后,提出了1) 译者应提高准确理解与运用语言的能力;2) 加强字幕翻译的译后审校工作;3) 以观众的观影体验为中心,合理安排字幕长度、停留时间、字体颜色等建议。