The fascial vessels of skin flap in leg were studied systematically and quantitatively so as to find out the survival mechanism of fasciocutaneous flap and provide morphological basis for clinical application. Methods...The fascial vessels of skin flap in leg were studied systematically and quantitatively so as to find out the survival mechanism of fasciocutaneous flap and provide morphological basis for clinical application. Methods: Thirteen legs from adultcadavers were observed and measured by dissection under operating and biological microscopes, tissue clearing, slice and imageanalysis. Results: There were four types of source artery in fascia of leg and the intermuscular septal cutaneous artery was predominant and often anstomosed as a chain. The source arteries had supra- and subfascial branches in deep fascia and the former wasmore in number and larger in diameter. In the same way, the vascular network was thicker in the suprafascial level than in the subfascial. Aa% of the deep fascia was larger than that of the superficial fascia. Conclusion: The deep fascial vasculature is the basis of the blood supply of fasciocutaneous flap in leg and the suprafascial vascular network is especially important. It is suggestedthat the fascial pedicle should be selected wilers the arterial chain exists.展开更多
Background Random flap is one kind of the most widely used skin flaps in reconstructive surgery; however, partial necrosis of its distal end remains a significant problem now. The aim of this study was to evaluate the...Background Random flap is one kind of the most widely used skin flaps in reconstructive surgery; however, partial necrosis of its distal end remains a significant problem now. The aim of this study was to evaluate the effect of hypoxia preconditioned bone marrow mesenchymal stem cells （HpBMSCs） transplantation on ultra-long random skin flap survival in rats. Methods Normoxic bone marrow mesenchymal stem cells （nBMSCs） were cultured under normoxia （20% 02） and HpBMSCs under hypoxia （1% 02） for 48 hours before transplantation. Thirty Sprague-Dawley rats were randomly divided into control group, nBMSCs group and HpBMSCs group with each consisting of 10 rats. Survival area of ultra-long random skin flap on the dorsal of rats was measured seven days after flap surgery and cell transplantation. Cell survival in vivo, microvessel density and vascular endothelial growth factor （VEGF） were evaluated by histological examination and enzyme-linked immunosorbent assay. Results Compared with other two groups, flap survival area in HpBMSCs group was significantly larger （P 〈0.05）. Microvessel density in HpBMSCs group （36.20-.8.19） was higher than that in nBMSCs group （30.01-.5.68） and control group （17.60..4.19） （P 〈0.05）. VEGF in HpBMSCs group （（300.05-.50.41） pg/g） was higher than those in nBMSCs group （（240.55_＋33.64） pg/g） and control group （（191.65..32.58） pg/g） （P 〈0.05）. Conclusion HpBMSCs transplantation improves ultra-long random skin flap survival via promoting angiogenesis of more survival cells.展开更多
文摘The fascial vessels of skin flap in leg were studied systematically and quantitatively so as to find out the survival mechanism of fasciocutaneous flap and provide morphological basis for clinical application. Methods: Thirteen legs from adultcadavers were observed and measured by dissection under operating and biological microscopes, tissue clearing, slice and imageanalysis. Results: There were four types of source artery in fascia of leg and the intermuscular septal cutaneous artery was predominant and often anstomosed as a chain. The source arteries had supra- and subfascial branches in deep fascia and the former wasmore in number and larger in diameter. In the same way, the vascular network was thicker in the suprafascial level than in the subfascial. Aa% of the deep fascia was larger than that of the superficial fascia. Conclusion: The deep fascial vasculature is the basis of the blood supply of fasciocutaneous flap in leg and the suprafascial vascular network is especially important. It is suggestedthat the fascial pedicle should be selected wilers the arterial chain exists.
基金The study was funded by grants from National Natural Science Foundation of China （No. 30900309, 30772269） and Graduate Independent Innovation Foundation of Shandong University （No. yzc09085）.
文摘Background Random flap is one kind of the most widely used skin flaps in reconstructive surgery; however, partial necrosis of its distal end remains a significant problem now. The aim of this study was to evaluate the effect of hypoxia preconditioned bone marrow mesenchymal stem cells （HpBMSCs） transplantation on ultra-long random skin flap survival in rats. Methods Normoxic bone marrow mesenchymal stem cells （nBMSCs） were cultured under normoxia （20% 02） and HpBMSCs under hypoxia （1% 02） for 48 hours before transplantation. Thirty Sprague-Dawley rats were randomly divided into control group, nBMSCs group and HpBMSCs group with each consisting of 10 rats. Survival area of ultra-long random skin flap on the dorsal of rats was measured seven days after flap surgery and cell transplantation. Cell survival in vivo, microvessel density and vascular endothelial growth factor （VEGF） were evaluated by histological examination and enzyme-linked immunosorbent assay. Results Compared with other two groups, flap survival area in HpBMSCs group was significantly larger （P 〈0.05）. Microvessel density in HpBMSCs group （36.20-.8.19） was higher than that in nBMSCs group （30.01-.5.68） and control group （17.60..4.19） （P 〈0.05）. VEGF in HpBMSCs group （（300.05-.50.41） pg/g） was higher than those in nBMSCs group （（240.55_＋33.64） pg/g） and control group （（191.65..32.58） pg/g） （P 〈0.05）. Conclusion HpBMSCs transplantation improves ultra-long random skin flap survival via promoting angiogenesis of more survival cells.
