Objective:To evaluate the effects of Mirazid and myrrh volatile oil on adult Fasciola gigantica(F.gigantica) under laboratory conditions.Methods:The effects of oleoresin extract of myrrh(Mirazid) and myrrh volatile oi...Objective:To evaluate the effects of Mirazid and myrrh volatile oil on adult Fasciola gigantica(F.gigantica) under laboratory conditions.Methods:The effects of oleoresin extract of myrrh(Mirazid) and myrrh volatile oil on the surface morphology of adult F.gigantica following treatment in vitro had been determined by scanning electron microscopy.The results were compared with those observed in the fluke tegument following incubation in triclabendazole sulphoxide(TCBZ-SO),active form.(Fasinex,Ciba-Geigy).Results:Observations of the efficacy of Mirazid oleoresin extract and myrrh volatile oil indicated that both products showed dosedependent anthelmintic efficacy.The anterior half of the fluke was consistently more severely affected than the posterior half.The surface changes induced by Mirazid oleoresin extract were less severe than those observed after exposure to either myrrh volatile oil or TCBZ-SO.Flukes showed swelling after these treatments,but its level and blebbing were much greater with myrrh volatile oil;in which patches of tegumental sloughing were observed in the apical cone and the posterior mid-body region of flukes.This was not observed after treatment with Mirazid oleoresin extract.Conclusions:The comparatively more disruption,observed in myrrh volatile oil exposed specimens,compared to that exposed to Mirazid oleoresin extract might suggest that the anthelmintic activity of Mirazid oleo resin extract was attributed to its content of volatile oil.So,increasing the concentration of myrrh volatile oil in Mirazid might possibly help to developing its anthelmintic activity._______________________________________________展开更多
Objective:To investigate the infection of Fasciola gigantka(F.gigantka)in domestic cattle from Chiang Mai province and molecular confirmation using ITS-2 region.Methods:The liver and gall bladder of Bubalus bubalis(B....Objective:To investigate the infection of Fasciola gigantka(F.gigantka)in domestic cattle from Chiang Mai province and molecular confirmation using ITS-2 region.Methods:The liver and gall bladder of Bubalus bubalis(B.bubalis)and Bos taurus(B.taunts)from slaughterhouses were examined adult worms and prevalence investigation.The species confirmation with phylogenetic analysis using ITS-2 sequences was performed by maximum likelihood and UPGMA methods.Results:The total prevalences of infection in B.bubalis and Bubalus taurus(B.taurus)were67.27%and 52.94%respectively.The respective prevalence in both B.bubalis and B.taurus were acquired from Doi-Saket,Muang,and Sanpatong districts,with 81.25%,62.50%and 60.00%for B.bubalis and 62.50%,50.00%and 47.06%for Bos taunts respectively.The species confirmation of F.gigantka and some related species by basing on maximum likelihood and UPGMA methods used,4 groups of trematodes were generated,first F.gigantka group including specimen of Chiang Mai,second 2 samples of F.hepatica,third group of 3 rumen flukes;Orthocoelium streptocoelium,F.elongatus and Paramphistomum epliclitum and fourth group of 3 minute intestinal flukes:Haplorchis taichui,Stellantchasmu falcatus.Haplorchoides sp.and liver fluke;Opisthorchis civerrini respectively.Conclusions:These results can be confirmed the Giant liver fluke which mainly caused fascioliasis in Chiang Mai was identified as F.gigantka and specimens were the same as those of F.gigantka recorded in olher different countries.Nucleotide sequence of ITS-2 region has been proven as effective diagnostic tool for the identification of F.gigantka.展开更多
Objective: To evaluate the effects of lead in the morphology and viability of Fasciola gigantica(F. gigantica)(giant liver fluke) isolated from infected livers of carabaos in vitro using the following concentrations o...Objective: To evaluate the effects of lead in the morphology and viability of Fasciola gigantica(F. gigantica)(giant liver fluke) isolated from infected livers of carabaos in vitro using the following concentrations of lead: 0, 100, 150 and 200 mg/L. Methods: In vitro viability and motility assay was conducted to evaluate the ef ects of lead using 1% methylene blue as the vital dye for assessment of the l ukes' viability. Results: Results indicate that F. gigantica can tolerate lead exposure as high as 200 mg/L with visible morphological variations. Upon exposure to lead, liver l ukes tend to curl and excrete black precipitates as a sign of physiological stress response. Furthermore, the lethal concentration(LC50) of lead against F. gigantica in vitro was 160 mg/L. Conclusions: In conclusion, tolerance of liver flukes to high levels of lead suggests its potential as a possible biomarker of environmental pollution.展开更多
Objective:To evaluated the histological changes within the tegument of adult Fasciola gigantica(F.gigantica)that led to the gross changes that were visible externally.Methods:The effects of oleoresin extract of myrrh(...Objective:To evaluated the histological changes within the tegument of adult Fasciola gigantica(F.gigantica)that led to the gross changes that were visible externally.Methods:The effects of oleoresin extract of myrrh(Mirazid^(?)),myrrh volatile oil and triclabendazole sulphoxide(reference drug)on the tegumental structure of adult F.gigantiea following treatment in vitro had been determined by light microscopy.Results:The internal changes in the tegument observed in this study were compatible with surface changes seen in the previous scanning electron microscopy study,using the same drugs.The swelling of tegumental syncytium was a particular feature of their action,but its level was much greater with myrrh volatile oil,in which vacuolization of the tegument and loss of spines were observed.Conclusions:The present study demonstrated the fasciocidal properties of Mirazid^(?)oleoresin extract,and it might be possible to reinforce its fasciocidal activity by increasing its content of myrrh volatile oil.展开更多
Fasciola hepatica is a parasite of clinical and veterinary importance which causes fascioliasis that leads to reduction in milk and meat production. Bile samples were centrifuged at 1500 rpm for ten (10) minutes in a ...Fasciola hepatica is a parasite of clinical and veterinary importance which causes fascioliasis that leads to reduction in milk and meat production. Bile samples were centrifuged at 1500 rpm for ten (10) minutes in a centrifuge machine and viewed microscopically to check for F. hepatica eggs. A total of 300 bile samples of cattle which included 155 males and 145 females were collected from the abattoir. Results were analyzed using chi-square (p > 0.05). The prevalence of F. gigantica and Dicrocoelium dentriticum is 33.0% (99) and 39.0% (117) respectively. Age prevalence of F. hepatica revealed that 0 - 2 years (33.7%, 29 cattle) were more infected than 2 - 4 years (32.7%, 70 cattle) while for D. dentriticum age 2 - 4 years were more infected than 0 - 2 years with prevalence of 40.2% (86) and 36.0% (31) respectively. No significant difference (P > 0.05) existed in prevalence in ages of the cattle. Out of the 300 bile samples examined, 22.3% (67 cattle) were co-infected with F. hepatica and D. dendriticum. Males were more co-infected than females having a prevalence of 24.5% (38 cattle) and 20.0% (29 cattle) respectively. Based on the age, samples of age 0 - 2 years were more co-infected than those of age 2 - 4 years with a prevalence of 23.3% (20 cattle) and 22.0% (47 cattle) respectively. The findings of this present study revealed that efforts to alleviate problems of animal health and productivity are yet to make any significant impact as this poses threat on human health. Investigation on the pattern of infections in cattle slaughtered should be strengthened.展开更多
对绵羊和水牛在感染大片吸虫后外周血白细胞总数计数和白细胞分类计数(嗜酸性细胞、嗜中性细胞、嗜碱性细胞、淋巴细胞和单核细胞)的特性进行研究。结果表明,绵羊对大片吸虫很不易感,水牛对大片吸虫很易感。未感染绵羊和水牛的白细胞总...对绵羊和水牛在感染大片吸虫后外周血白细胞总数计数和白细胞分类计数(嗜酸性细胞、嗜中性细胞、嗜碱性细胞、淋巴细胞和单核细胞)的特性进行研究。结果表明,绵羊对大片吸虫很不易感,水牛对大片吸虫很易感。未感染绵羊和水牛的白细胞总数有较大的差距,分别为5 956(±825)个细胞/mm3血液和12 657(±1 053)个细胞/mm3血液。未感染绵羊和水牛的嗜酸性细胞占白细胞总数的百分比小于5%。淋巴细胞占的比例最大,绵羊为59.5%,水牛达70.8%。感染绵羊和未感染绵羊的白细胞总数、嗜酸性细胞计数在感染过程中总体上存在显著差异,感染后绵羊的嗜酸性细胞的数量迅速升高,在感染后4周(4W P I)达到高峰。而感染水牛和未感染水牛白细胞总数差异不显著,但嗜酸性细胞存在显著差异,感染后水牛的嗜酸性细胞的数量升高缓慢,到8 W P I达到高峰。结果提示嗜酸性细胞在感染绵羊中更加有效地参与了杀灭大片吸虫。展开更多
文摘Objective:To evaluate the effects of Mirazid and myrrh volatile oil on adult Fasciola gigantica(F.gigantica) under laboratory conditions.Methods:The effects of oleoresin extract of myrrh(Mirazid) and myrrh volatile oil on the surface morphology of adult F.gigantica following treatment in vitro had been determined by scanning electron microscopy.The results were compared with those observed in the fluke tegument following incubation in triclabendazole sulphoxide(TCBZ-SO),active form.(Fasinex,Ciba-Geigy).Results:Observations of the efficacy of Mirazid oleoresin extract and myrrh volatile oil indicated that both products showed dosedependent anthelmintic efficacy.The anterior half of the fluke was consistently more severely affected than the posterior half.The surface changes induced by Mirazid oleoresin extract were less severe than those observed after exposure to either myrrh volatile oil or TCBZ-SO.Flukes showed swelling after these treatments,but its level and blebbing were much greater with myrrh volatile oil;in which patches of tegumental sloughing were observed in the apical cone and the posterior mid-body region of flukes.This was not observed after treatment with Mirazid oleoresin extract.Conclusions:The comparatively more disruption,observed in myrrh volatile oil exposed specimens,compared to that exposed to Mirazid oleoresin extract might suggest that the anthelmintic activity of Mirazid oleo resin extract was attributed to its content of volatile oil.So,increasing the concentration of myrrh volatile oil in Mirazid might possibly help to developing its anthelmintic activity._______________________________________________
文摘Objective:To investigate the infection of Fasciola gigantka(F.gigantka)in domestic cattle from Chiang Mai province and molecular confirmation using ITS-2 region.Methods:The liver and gall bladder of Bubalus bubalis(B.bubalis)and Bos taurus(B.taunts)from slaughterhouses were examined adult worms and prevalence investigation.The species confirmation with phylogenetic analysis using ITS-2 sequences was performed by maximum likelihood and UPGMA methods.Results:The total prevalences of infection in B.bubalis and Bubalus taurus(B.taurus)were67.27%and 52.94%respectively.The respective prevalence in both B.bubalis and B.taurus were acquired from Doi-Saket,Muang,and Sanpatong districts,with 81.25%,62.50%and 60.00%for B.bubalis and 62.50%,50.00%and 47.06%for Bos taunts respectively.The species confirmation of F.gigantka and some related species by basing on maximum likelihood and UPGMA methods used,4 groups of trematodes were generated,first F.gigantka group including specimen of Chiang Mai,second 2 samples of F.hepatica,third group of 3 rumen flukes;Orthocoelium streptocoelium,F.elongatus and Paramphistomum epliclitum and fourth group of 3 minute intestinal flukes:Haplorchis taichui,Stellantchasmu falcatus.Haplorchoides sp.and liver fluke;Opisthorchis civerrini respectively.Conclusions:These results can be confirmed the Giant liver fluke which mainly caused fascioliasis in Chiang Mai was identified as F.gigantka and specimens were the same as those of F.gigantka recorded in olher different countries.Nucleotide sequence of ITS-2 region has been proven as effective diagnostic tool for the identification of F.gigantka.
文摘Objective: To evaluate the effects of lead in the morphology and viability of Fasciola gigantica(F. gigantica)(giant liver fluke) isolated from infected livers of carabaos in vitro using the following concentrations of lead: 0, 100, 150 and 200 mg/L. Methods: In vitro viability and motility assay was conducted to evaluate the ef ects of lead using 1% methylene blue as the vital dye for assessment of the l ukes' viability. Results: Results indicate that F. gigantica can tolerate lead exposure as high as 200 mg/L with visible morphological variations. Upon exposure to lead, liver l ukes tend to curl and excrete black precipitates as a sign of physiological stress response. Furthermore, the lethal concentration(LC50) of lead against F. gigantica in vitro was 160 mg/L. Conclusions: In conclusion, tolerance of liver flukes to high levels of lead suggests its potential as a possible biomarker of environmental pollution.
基金Supported by National Research Center,Egypt,through the Parasitology and Animal Diseases Department(Grant No.9040203)
文摘Objective:To evaluated the histological changes within the tegument of adult Fasciola gigantica(F.gigantica)that led to the gross changes that were visible externally.Methods:The effects of oleoresin extract of myrrh(Mirazid^(?)),myrrh volatile oil and triclabendazole sulphoxide(reference drug)on the tegumental structure of adult F.gigantiea following treatment in vitro had been determined by light microscopy.Results:The internal changes in the tegument observed in this study were compatible with surface changes seen in the previous scanning electron microscopy study,using the same drugs.The swelling of tegumental syncytium was a particular feature of their action,but its level was much greater with myrrh volatile oil,in which vacuolization of the tegument and loss of spines were observed.Conclusions:The present study demonstrated the fasciocidal properties of Mirazid^(?)oleoresin extract,and it might be possible to reinforce its fasciocidal activity by increasing its content of myrrh volatile oil.
文摘Fasciola hepatica is a parasite of clinical and veterinary importance which causes fascioliasis that leads to reduction in milk and meat production. Bile samples were centrifuged at 1500 rpm for ten (10) minutes in a centrifuge machine and viewed microscopically to check for F. hepatica eggs. A total of 300 bile samples of cattle which included 155 males and 145 females were collected from the abattoir. Results were analyzed using chi-square (p > 0.05). The prevalence of F. gigantica and Dicrocoelium dentriticum is 33.0% (99) and 39.0% (117) respectively. Age prevalence of F. hepatica revealed that 0 - 2 years (33.7%, 29 cattle) were more infected than 2 - 4 years (32.7%, 70 cattle) while for D. dentriticum age 2 - 4 years were more infected than 0 - 2 years with prevalence of 40.2% (86) and 36.0% (31) respectively. No significant difference (P > 0.05) existed in prevalence in ages of the cattle. Out of the 300 bile samples examined, 22.3% (67 cattle) were co-infected with F. hepatica and D. dendriticum. Males were more co-infected than females having a prevalence of 24.5% (38 cattle) and 20.0% (29 cattle) respectively. Based on the age, samples of age 0 - 2 years were more co-infected than those of age 2 - 4 years with a prevalence of 23.3% (20 cattle) and 22.0% (47 cattle) respectively. The findings of this present study revealed that efforts to alleviate problems of animal health and productivity are yet to make any significant impact as this poses threat on human health. Investigation on the pattern of infections in cattle slaughtered should be strengthened.
文摘对绵羊和水牛在感染大片吸虫后外周血白细胞总数计数和白细胞分类计数(嗜酸性细胞、嗜中性细胞、嗜碱性细胞、淋巴细胞和单核细胞)的特性进行研究。结果表明,绵羊对大片吸虫很不易感,水牛对大片吸虫很易感。未感染绵羊和水牛的白细胞总数有较大的差距,分别为5 956(±825)个细胞/mm3血液和12 657(±1 053)个细胞/mm3血液。未感染绵羊和水牛的嗜酸性细胞占白细胞总数的百分比小于5%。淋巴细胞占的比例最大,绵羊为59.5%,水牛达70.8%。感染绵羊和未感染绵羊的白细胞总数、嗜酸性细胞计数在感染过程中总体上存在显著差异,感染后绵羊的嗜酸性细胞的数量迅速升高,在感染后4周(4W P I)达到高峰。而感染水牛和未感染水牛白细胞总数差异不显著,但嗜酸性细胞存在显著差异,感染后水牛的嗜酸性细胞的数量升高缓慢,到8 W P I达到高峰。结果提示嗜酸性细胞在感染绵羊中更加有效地参与了杀灭大片吸虫。
