Risk factors for small bowel angioectasia:The impact of visceral fat accumulation

AIM: To investigate visceral fat accumulation in association with the risk of small bowel angioectasia.METHODS: We retrospectively investigated 198 consecutive patients who underwent both capsule endoscopy and CT for investigation of obscure gastrointestinal bleeding(OGIB) from January 2009 to September 2013. The visceral fat area(VFA) and subcutaneous fat area were measured by CT, and information on comorbidities, body mass index, and medications was obtained from their medical records.Logistic regression analysis was used to evaluate associations.RESULTS: Capsule endoscopy revealed small bowel angioectasia in 18/198(9.1%) patients with OGIB.Compared to patients without small bowel angioectasia,those with small bowel angioectasia had a significantly higher VFA(96 ± 76.0 cm2 vs 63.4 ±51.5 cm2, P = 0.016) and a higher prevalence of liver cirrhosis(61% vs 22%, P < 0.001). The proportion of patients with chronic renal failure was higher in patients with small bowel angioectasia(22% vs 9%,P = 0.11). There were no significant differences in subcutaneous fat area or waist circumference. The prevalence of small bowel angioectasia progressively increased according to the VFA. Multivariate analysis showed that the VFA [odd ratio(OR) for each 10-cm2 increment = 1.1; [95% confidence interval(CI):1.02-1.19; P = 0.021] and liver cirrhosis(OR = 6.1,95%CI: 2.2-18.5; P < 0.001) were significant risk factors for small bowel angioectasia.CONCLUSION: VFA is positively associated with theprevalence of small bowel angioectasia, for which VFA and liver cirrhosis are independent risk factors in patients with OGIB.

Factors associated with visceral fat accumulation in the general population in Okinawa, Japan

AIM:To investigate the clinical and biochemical factors associated with visceral fat accumulation in the general population.METHODS:We enrolled 1004 subjects who underwent a medical health checkup between April 2008 and March 2009.The medical health checkup included the following tests:Height,body weight,waist circumference(WC),systolic blood pressure,diastolic blood pressure,urinalysis,blood-cell counts,blood chemistry,electrocardiography,chest radiography,and abdominal computed tomography(CT)for visceral fat accumulation.The patients’medical history and lifestyle factors were collected privately by nurses using a selfadministered questionnaire,and they included questions regarding physical activity,sleep duration,dietary habits,smoking,and alcohol consumption.visceral fat area(VFA)was defined as the sum of the intraperitoneal fat area at the level of the umbilicus with CT density in the range of-150 to-50 Hounsfield units.RESULTS:The mean age and body mass index(BMI)of the study subjects were 57.0 years and 24.4 kg/m2.In both male and females,v FA was significantly andpositively correlated with WC(r=0.532,P<0.01;r=0.612,P<0.01).Subjects with high levels of v FA were primarily male with significantly higher age,height,body weight,BMI,systolic blood pressure(BP),diastolic BP,and hemoglobin in all subjects(P<0.05).A multivariate logistic regression analysis revealed that vF A had a positive relationship with age≥56,BMI≥25 kg/m2,and triglyceride level≥149 in males(P<0.05),whereas it had a positive relationship with age≥58,BMI≥24.4 kg/m2,high-density lipoprotein cholesterol level<40 mg/d L,and current drinking in females(P<0.05).CONCLUSION:These results suggest that gender differences exist in the clinical and biochemical parameters associated with visceral fat accumulation.

Lysine 2-hydroxyisobutyrylation levels determined adipogenesis and fat accumulation in adipose tissue in pigs

Background Excessive backfat deposition lowering carcass grade is a major concern in the pig industry,especially in most breeds of obese type pigs.The mechanisms involved in adipogenesis and fat accumulation in pigs remain unclear.Lysine 2-hydroxyisobutyrylation(Khib),is a novel protein post-translational modification(PTM),which play an important role in transcription,energy metabolism and metastasis of cancer cells,but its role in adipogenesis and fat accumulation has not been shown.Results In this study,we first analyzed the modification levels of acetylation(Kac),Khib,crotonylation(Kcr)and succinylation(Ksu)of fibro-adipogenic progenitors(FAPs),myogenic precursors(Myo)and mesenchymal stem cells(MSCs)with varied differentiation potential,and found that only Khib modification in FAPs was significantly higher than that in MSCs.Consistently,in parallel with its regulatory enzymes lysine acetyltransferase 5(KAT5)and histone deacetylase 2(HDAC2)protein levels,the Khib levels increased quadratically(P<0.01)during adipogenic differentiation of FAPs.KAT5 knockdown in FAPs inhibited adipogenic differentiation,while HDAC2 knockdown enhanced adipogenic differentiation.We also demonstrated that Khib modification favored to adipogenic differentiation and fat accumulation by comparing Khib levels in FAPs and backfat tissues both derived from obese-type pigs(Laiwu pigs)and lean-type pigs(Duroc pigs),respectively.Accordingly,the expression patterns of KAT5 and HDAC2 matched well to the degree of backfat accumulation in obese-and lean-type pigs.Conclusions From the perspective of protein translational modification,we are the first to reveal the role of Khib in adipogenesis and fat deposition in pigs,and provided new clues for the improvement of fat accumulation and distribution as expected via genetic selection and nutritional strategy in obese-type pigs.

Polysaccharide-rich extract of Potentilla anserina ameliorates nonalcoholic fatty liver disease in free fatty acid-induced HepG2 cells and high-fat/sugar diet-fed mice

Potentilla anserina L.(PA)belongs to the Rosaceae family,is a common edible plant in the Qinghai-Tibet Plateau areas of China.This study elucidates the mechanism upon which crude polysaccharide of PA(PAP)on fat accumulation in HepG2 cells stimulated by oleic acid(OA)and high fat high sugar induced mice.The result revealed that PAP inhibited lipid accumulation in obese mice and ameliorated the degree of damage in OA-induced HepG2 cells.Specifically,compared to the control group,the TG and TC levels were decreased in cells and mice serum,the aspartate transaminase and alamine aminotransferase contents were declined in liver of obese mice by PAP treatment.The expressions of adipogenic genes of SREBP-1c,C/EBPα,PPARγ,and FAS were inhibited after PAP treatment.Moreover,PAP increased the mRNA levels of CPT-1 and PPARα,which were involved in fatty acid oxidation.The present results indicated the PAP could alleviate the damage of liver associated with obesity and PAP treatment might provide a dietary therapeutic option for the treatment of hyperlipidemia.

Liver fat accumulation measured by high-speed T2-corrected multiecho magnetic resonance spectroscopy can predict risk of cholelithiasis

BACKGROUND Liver fat accumulation is associated with increased cholesterol synthesis and hypersecretion of biliary cholesterol,which may be related to the development of cholelithiasis.AIM To investigate whether liver fat accumulation measured by high-speed T2-corrected multi-echo magnetic resonance spectroscopy(MRS)is a risk factor for cholelithiasis.METHODS Forty patients with cholelithiasis and thirty-one healthy controls were retrospectively enrolled.The participants underwent high-speed T2-corrected multi-echo single-voxel MRS of the liver at a 3T MR scanner.The proton density fat fraction(PDFF)and R2 value were calculated.Serum parameters and waist circumference(WC)were recorded.Spearman’s correlation analysis was used to analyze the relationship between PDFF,R2,and WC values.Multivariate logistic regression analysis was carried out to determine the significant predictors of the risk of cholelithiasis.Receiver operating characteristic curve(ROC)analysis was used to evaluate the discriminative performance of significant predictors.RESULTS Patients with cholelithiasis had higher PDFF,R2,and WC values compared with healthy controls(5.8%±4.2%vs 3.3%±2.4%,P=0.001;50.4±24.8/s vs 38.3±8.8/s,P=0.034;85.3±9.0 cm vs 81.0±6.9 cm,P=0.030;respectively).Liver iron concentration extrapolated from R2 values was significantly higher in the cholelithiasis group(2.21±2.17 mg/g dry tissue vs 1.22±0.49 mg/g dry tissue,P=0.034)than in the healthy group.PDFF was positively correlated with WC(r=0.502,P<0.001)and R2(r=0.425,P<0.001).Multivariate logistic regression analysis showed that only PDFF was an independent risk factor for cholelithiasis(odds ratio=1.79,95%CI:1.22-2.62,P=0.003).ROC analysis showed that the area under the curve of PDFF was 0.723 for discriminating cholelithiasis from healthy controls,with a sensitivity of 55.0%and specificity of 83.9%when the cut-off value of PDFF was 4.4%.CONCLUSION PDFF derived from high speed T2-corrected multi-echo MRS can predict the risk of cholelithiasis.

Abdominal Fat Accumulation with Hyperuricemia and Hypercholesterolemia Quail Model Induced by High Fat Diet

Objective To establish abdominal fat accumulation with hyperuricemia and hypercholesterolemia quail model fed with high fat diet. And then to investigate the pathological characteristics of this quail model. Methods Thirty Longcheng quails were randomly divided into two groups： control group and model group （n=15）. The control group quails were fed with normal diet and model group quails were fed with high fat diet for 14 days. After a 12-hour overnight fast, liver and abdominal fat at euthanasia as well as serum were collected. The levels of serum uric acid, total cholesterol, high density lipoprotein cholesterol （HDL-C）, low density lipoprotein cholesterol （LDL-C）, triglyceride, free fatty acid （FFA）, and blood glucose were assayed. The activity changes of adenosine deaminase （ADA）, xanthine oxidase （XOD）, lipoprotein lipase （LPL）, hepatic lipase （HL）, and fatty acid synthetase （FAS） were analyzed. Results Compared with control group, the abdominal fat content （0.74±0.63 vs. 1.36±0.65 g, P〈0.05） and abdominal fat index （0.44%±0.30% vs. 0.85%±0.30%, P〈0.01） as well as live lipid index （3.61%±0.65% vs. 11.33%±2.14%, P〈0.01） in model group significantly increased; the levels of serum uric acid （210.61±94,76 vs. 304.25±141.94 /amol/L, P〈0.05）, total cholesterol （4.20±0.51 vs. 20.10±11.25 mmol/L, P〈0.01）, LDL-C （1.16±0.29 vs. 10.78±6.48 mmol/L, P〈0.01）, and FFA （0.39±0.14 vs. 0.55±0.15 mmol/L, P〈0.01） in model group significantly increased; HDL-C （5.85±0.95 vs. 4.14±2.03 mmol/L, P〈0.05） significantly decreased; the levels of triglyceride and blood glucose had no significant changes （P〉0.05）; the activities of ADA （9.71±3.05 vs. 17.19±5.10 U/ml, P〈0.01） and XOD （10.58±6,88 vs. 19.22＋9.44 U/L, P〈0.01） in model group significantly increased; and FAS, LPL, HL had no significant changes （P〉0.05）. Conclusions High fat diet can induce abdominal fat accumulation with hyperuricemia and hypercholesterolemia quail model. The changes of uric acid and lipid metabolic enzyme activities may he the pathological mechanism of abdominal fat accumulation with hyperuricemia and hypercholesterolemia.

Dietary Supplementation with Sea Cucumber Saponins and Exercise Can Significantly Suppress Adipose Accumulation in Mice Fed with High-Fat Diet

Dietary supplementation with sea cucumber saponins(SCS)and exercise have been confirmed to be effective in pre-venting the development of obesity and its related diseases.However,the combined effectiveness of these interventions has not been explored.Here,we studied whether the beneficial influences of exercise could be further enhanced by dietary supplementation with SCS in high-fat diet-fed KM(Kunming)mice.Mice were randomly divided into four groups,including the high-fat diet group(HF),the SCS group(HF-S),the exercise group(HF-E),and the combination of dietary SCS and exercise group(HF-S+E).There were eight mice in every group.The results demonstrated that the combination of dietary SCS and exercise could synergistically reduce fat accumulation.In particular,white adipose tissue decreased by 63%in the HF-S+E group compared with that in the HF group.SCS supplementation with exercise also improved peripheral markers,such as serum parameters and hepatic TG levels.Further mechani-cal testing indicated that the combined effects of dietary SCS and exercise on inhibiting fat accumulation might be attributed to the inhibition of lipid synthesis in the liver and the activation of lipolysis in white adipose tissue to increase energy consumption.

Moderate quantity of lard mixed with sunflower oil attenuate lipid accumulation in mice

Lard,a fat rich in saturated fatty acids(SFAs),is regarded as a risk factor for metabolic diseases.In the present study,effect of different lard blended with sunflower oil diets on lipid accumulation in adipose tissue,liver,and serum by mouse model was researched.Body weight,body fat percentage,cross-sectional area of adipocytes,liver triglycerides(TGs),and oil red stained area in mice liver of lard blend sunflower oil(L-SFO)group were significantly lower than those of sunflower oil(SFO)group,whereas no significant differences were observed between mice of lard and L-SFO groups.Serum TG and free fatty acid levels were significantly lower in L-SFO group than in other two groups.Furthermore,data showed that sunflower oil decreased contents of hormonesensitive lipase and carnitine palmitoyl transferase 1(CPT-1)and increased fatty acid synthase activity in liver tissue.A mixture of lard and sunflower oil rather than only sunflower oil or lard might promote body fat loss and reduce lipid accumulation in adipose tissue,serum,and liver by promoting hydrolysis of TG,increasingβ-oxidation of fatty acids.These data suggested that mixing lard and vegetable oil(e.g.sunflower oil)for cooking,or alternate using lard and vegetable oil could be beneficial for reducing body fat.

Leucosceptroid B from glandular trichomes of Leucosceptrum canum reduces fat accumulation in Caenorhabditis elegans through suppressing unsaturated fatty acid biosynthesis

Obesity that is highly associated with numerous metabolic diseases has become a global health issue nowdays.Plant sesterterpenoids are an important group of natural products with great potential;thus,their bioactivities deserve extensive exploration.RNA-seq analysis indicated that leucosceptroid B,a sesterterpenoid previously discovered from the glandular trichomes of Leucosceptrum canum,significantly regulated the expression of 10 genes involved in lipid metabolism in Caenorhabditis elegans.Furthermore,leucosceptroid B was found to reduce fat storage,and downregulate the expression of two stearoyl-CoA desaturase(SCD)genes fat-6 and fat-7,and a fatty acid elongase gene elo-2 in wild-type C.elegans.In addition,leucosceptroid B significantly decreased fat accumulation in both fat-6 and fat-7 mutant worms but did not affect the fat storage of fat-6;fat-7 double mutant.These findings indicated that leucosceptroid B reduced fat storage depending on the downregulated expression of fat-6,fat-7 and elo-2 and thereby inhibiting the biosynthesis of the corresponding unsaturated fatty acid.These findings provide new insights into the development and utilization of plant sesterterpenoids as potential antilipemic agents.

OSAHS患者复合脂质指标与冠心病发病风险的进展

近年来,心血管疾病的发病率逐步上升,阻塞性睡眠呼吸暂停低通气综合征(OSAHS)作为心血管疾病的独立危险因素,与冠心病的发生息息相关,因此预防OSAHS能提升患者生活质量和减轻疾病负担。然而,因存在多种共同的危险因素,OSAHS患者心血管疾病的风险评估变得困难。最近大量研究表明,复合脂质指标即血浆致动脉粥样硬化指数(AIP)、内脏脂肪指数(VAI)和脂质蓄积指数(LAP)能更好地预测心血管疾病。目前,OSAHS患者中,关于复合脂质指标与冠心病的相关性研究并不多见。因此,该文通过阐述OSAHS、冠心病、复合脂质指标之间的关系,进一步明确复合脂质指标来预测OSAHS患者冠心病发病风险的意义,为临床提供准确的风险评估策略。

2型糖尿病患者肾脏脂肪定量测量:Dixon与HISTO MRS技术的比较

目的比较基于六回波水脂分离(Dixon)和高速T2校正多回波磁共振波谱(high-speed T2-crrected multiecho magnetic resonance spectroscopic,HISTO MRS)技术脂肪定量指标在肾脏的一致性并探讨其对肾功能损害的预测能力,为2型糖尿病(type 2 diabetic mellitus,T2DM)患者肾脏脂毒性损害程度判定及治疗监测提供依据。材料与方法前瞻性纳入172名T2DM患者和55名健康受试者。所有受试者接受Dixon与HISTO MRS腹部3 T MRI检查,获得肾脏质子密度脂肪分数(proton density fat fraction,PDFF),分别记为D-PDFF和H-PDFF。使用组内相关系数评价两种技术的一致性;根据有无T2DM和肾小球滤过率(estimated glomerular filtration rate,eGFR)将所有受试者分为健康受试者、T2DM患者肾功能正常组(N-T2DM)、T2DM患者肾功能轻中度受损组(M-T2DM)、T2DM患者肾功能严重受损组(S-T2DM),并比较组间差异性;应用Pearson相关性分析和多元线性回归明确D-PDFF和H-PDFF是否为eGFR降低的独立危险因素。结果D-PDFF和H-PDFF的组内相关系数为0.185。校正混杂因素后,健康受试者和T2DM患者D-PDFF的差异有统计学意义(P<0.001),H-PDFF的差异无统计学意义(P>0.05)。校正后健康受试者与N-T2DM组、健康受试者与M-T2DM组、N-T2DM与M-T2DM组的D-PDFF的差异均有统计学意义(P均≤0.001);H-PDFF在各组间的差异均无统计学意义(P均>0.05)。在校正混杂因素后D-PDFF与eGFR的升高独立负相关(β=-0.168,P=0.016),是肾功能损害的独立危险因素。H-PDFF值与eGFR无相关性(β=-0.008,P=0.918)。结论两种MRI脂肪定量测量技术在肾脏的一致性差,基于Dixon的PDFF更能反映T2DM患者肾脏脂肪沉积增加和肾功能损害,可能更适用于T2DM患者的肾脏脂肪沉积监测。

泽漆醇提取物抗氧化活性及对油酸诱导HepG2细胞脂肪堆积的影响

为了探究泽漆醇提取物体外抗氧化作用及其对油酸诱导HepG2细胞脂肪堆积的影响。本研究采用浸渍法分别以25%、50%、75%甲醇和乙醇对泽漆粉末进行提取,评估不同泽漆提取物的体外抗氧化活性,并测定其总酚和总黄酮含量。建立HepG2细胞脂肪堆积模型,不同浓度(20、40、60μg/mL)泽漆50%乙醇提取物处理24 h,观察细胞内脂滴形成情况;测定细胞中甘油三脂(triglyceride,TG)含量、总谷胱甘肽(glutathione,GSH)、超氧化物歧化酶(superoxide dismutase,SOD)的活性及总抗氧化能力(total antioxidant capacity,T-AOC)。结果表明,泽漆醇提取物均具有抗氧化活性,且有浓度依赖性,其中50%乙醇提取物在一定浓度范围内抗氧化效果最佳(P<0.05),且总酚(8.813%±1.344%)和总黄酮(17.938%±0.819%)含量也较高。与模型组比较,泽漆50%乙醇提取物能够降低HepG2细胞内脂滴和TG值,提高细胞GSH、T-AOC含量和SOD活性(P<0.05),具有浓度依赖性。综上可知,50%乙醇提取物可以抑制油酸诱导的HepG2细胞脂肪堆积,并增强细胞内源性抗氧化能力。

糖尿病前期患者肥胖相关指标特征及与T2DM发生的相关性分析

目的探究糖尿病前期患者内脏脂肪指数(VAI)、脂质蓄积指数(LAP)、甘油三酯-葡萄糖指数(TyG)等肥胖相关指标的特征及其与2型糖尿病(T2DM)发生的相关性。方法选取2020年1月至2022年12月于该院收治的64例T2DM患者作为观察组,同期64例糖尿病前期患者作为对照组。对比两组患者一般临床资料、糖脂代谢相关指标[总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、血糖(FPG)及糖化血红蛋白(HbA1c)]、肥胖及胰岛功能相关指标[体重指数(BMI)、身体圆度指数(BRI)、VAI、LAP、TyG]。通过相关性分析及Logistic回归分析筛选T2DM发生的危险因素,通过受试者工作特征(ROC)曲线及曲线下面积(AUC)评价各危险因素对T2DM发生的预测价值。结果观察组患者腰围、TG、FPG、HbA1c、BMI、VAI、LAP、BRI及TyG均明显高于对照组(P<0.05)。相关性分析表明,患者BRI与FPG呈正相关(P<0.05),BMI与HbA1c呈正相关(P<0.05)。二元Logistic回归分析显示,患者BMI、VAI、LAP、TyG升高均是T2DM发生的独立危险因素(P<0.05),其中LAP水平对T2DM发生的预测价值最高,AUC为0.854。结论糖尿病前期患者BMI、VAI、LAP、TyG升高可能与T2DM的发生呈正相关,早期监测上述指标对预测糖尿病前期患者预后及疾病转归具有一定临床意义。

核桃粕酶解物抑制脂质积累的机制

利用分化的3T3-L1脂肪细胞和高脂饮食(HFD)诱导的肥胖C57BL/6小鼠模型,研究核桃粕酶解物的抗肥胖作用。采用油红O脂肪染色法检测脂肪细胞脂质积累情况,采用试剂盒检测细胞甘油三脂(TG)、甘油(Gly)和游离脂肪酸(FFA)含量,采用实时荧光定量PCR(PCR RT-qPCR)检测脂肪合成相关基因(mRNA)的表达水平。结果表明,核桃粕酶解物显著降低脂肪细胞内脂滴聚积,显著降低脂肪细胞内TG和FFA的含量,增加细胞上清液中Gly含量,同时核桃粕酶解物显著降低脂肪细胞中脂肪合成相关基因PPAR-γ和C/EBPαmRNA表达水平;当核桃粕酶解物的质量浓度达到50μg/mL时,TG和FFA分别降低了40.14%和39.50%,Gly水平增加了35.12%;PPAR-γ和C/EBPα的mRNA表达水平分别降低了31.42%和55.25%。此外,核桃粕酶解物显著降低HFD小鼠的体质量、脂肪组织质量和食物摄入量。研究表明,核桃粕酶解物可以抑制3T3-L1脂肪细胞脂质积累,其作用机制与抑制脂质合成相关基因表达相关。此外,其还具有抑制HFD脂质积累的作用。

中国中老年女性脂质积累产物和内脏脂肪指数与膝骨关节炎的关联性

目的探讨中国中老年女性脂质积累产物(LAP)和内脏脂肪指数(VAI)与膝骨关节炎(KOA)的关联性。方法收集中国健康与养老追踪调查(CHARLS)数据库2011年的数据。采用Logistic回归和受试者工作特征(ROC)曲线分析LAP、VAI与KOA发病的关联。结果中国≥45岁女性KOA的患病率为34.01%(1257/3696)。单因素Logistic回归分析显示,LAP(OR=1.013,95%CI=1.012~1.014)、VAL(OR=1.137,95%CI=1.117~1.158)与KOA发病呈正相关。在调整混杂因素后,LAP(OR=1.003,95%CI=1.002~1.004)和VAI(OR=1.048,95%CI=1.029~1.069)仍与KOA发病呈显著正相关。LAP的ROC曲线下面积(AUC)为0.725(95%CI=0.709~0.741),VAI的AUC为0.630(95%CI=0.613~0.647)。结论LAP和VAI增高是KOA发病的危险因素。LAP对中老年女性KOA的发病有一定的预测价值。

肥胖测量指标在预测代谢相关脂肪性肝病健康风险中的应用价值

目的评估肥胖测量指标对代谢相关脂肪性肝病(Metabolic-associated fatty liver disease,MAFLD)健康风险的预测价值。方法选取2023年6月1日-12月31日在新疆医科大学第一附属医院健康管理中心体检的49155例体检者,根据诊断标准,将其分为MAFLD组和非MAFLD组,并计算体质指数(BMI)、腰臀比(WHR)、内脏脂肪指数(VAI)、脂质蓄积指数(LAP)。BMI分为正常、超重、肥胖组;腰围(WC)分为正常、中心性肥胖前期和中心性肥胖组。采用Logistic回归模型分析性别亚组中肥胖测量指标及生化指标的差异。绘制受试者工作特征(ROC)曲线评估各指标对MAFLD的预测价值。结果共检出MAFLD 23765例(48.35%),其中男性18030例(75.87%),女性5735例(24.13%)。同一性别中,MAFLD组除高密度脂蛋白胆固醇(HDL-C)水平低于非MAFLD组外,其余指标水平均高于非MAFLD组(P均<0.001);校正混杂因素后Logistic模型显示,MAFLD发生风险随BMI、WC、WHR、VAI、LAP水平增高而增高;ROC分析发现,BMI、WHR、VAI和LAP预测不同性别组MAFLD的曲线下面积(AUC)均大于0.7,其中LAP的AUC值最大,且预测女性的AUC值[0.896(0.891~0.900)]大于男性AUC值[0.831(0.826~0.836)],最佳截断值为25.49、39.26(P均<0.05);BMI×LAP联合模型在不同性别中预测价值最高,AUC值分别为男性0.846(0.841~0.850)、女性0.908(0.904~0.913)(P均<0.05)。结论MAFLD发生风险随BMI、WHR、VAI、LAP水平升高而增高,LAP相较于其他指标预测价值更高,尤其在女性中。BMI×LAP可作为不同性别中MAFLD健康风险评估的有力工具。

无创双极射频技术在下面部衰老中的应用研究

目的探索无创双极射频技术在下面部和下颌缘处皮肤松弛、脂肪堆积中的临床应用效果。方法观察2021年5月至2022年8月,因下面部和下颌缘处皮肤松弛、脂肪堆积于重庆医科大学附属第一医院医疗美容科行无创射频治疗的患者60例评估患者治疗过程中的疼痛程度、治疗后并发症。于治疗结束后3个月,评估下面部皮肤松弛和脂肪堆积改善程度。结果治疗过程中大部分患者疼痛评分均在1-3分之间。治疗过程中有8例患者出现皮下轻度瘀斑,无烫伤、神经损伤、瘢痕形成发生。治疗结束后3个月,由2名非治疗组高年资医生对患者进行治疗前及治疗后3月面部松弛程度量表评分,下面部松弛程度平均得分从治疗前4(4,5)分下降至治疗后三月的3(2,3)分(P<0.01),颈部松弛程度平均得分从治疗前5(4,5)分下降至治疗后三月的4(3,4)分(P<0.01)。下面部及颈部松弛程度治疗前后评分有统计学差异。结论对于轻中度下面部和下颌缘处皮肤松弛、脂肪堆积的患者无创射频技术不仅能取得较好治疗效果,同时也有无创伤、无误工期、患者满意度高等优点,具有较好的临床应用效果。

春夏播玉米籽粒蛋白质、脂肪和淀粉含量动态累积与分配差异性研究

【目的】研究春夏播玉米籽粒蛋白质、脂肪和淀粉含量动态累积与分配差异性特征,为专用优质玉米品种选育与生产提供参考。【方法】以驻玉216、驻玉902、驻玉927、郑单958、裕丰303为试验材料,授粉后12 d每隔7 d取果穗内籽粒,烘干至恒重,利用近红外光谱技术快速检测玉米籽粒蛋白质、脂肪、淀粉含量,分析籽粒蛋白质、脂肪、淀粉动态累积相关关系,比较春夏播玉米籽粒蛋白质、脂肪与淀粉含量动态累积与分配的差异性。【结果】玉米籽粒蛋白质含量春播先急剧下降后平稳变化,夏播先急剧下降后又升高,呈波浪式变化;春夏播玉米籽粒脂肪含量先上升后下降,淀粉含量随籽粒发育基本呈渐进式增加,蛋脂总量整体呈逐步下降趋势。春夏播玉米籽粒蛋白质、脂肪与淀粉累积速率动态呈锯齿状变化。籽粒蛋白质、脂肪和淀粉分配春播授粉后12~19 d整体呈现为淀粉>蛋白质>其他>脂肪,授粉后26~47 d基本呈现为淀粉>其他>蛋白质>脂肪;夏播授粉后12~18 d整体呈现为淀粉>蛋白质>其他>脂肪,授粉后19~47 d基本呈现为淀粉>其他>蛋白质>脂肪。相关性分析表明,籽粒蛋白质、脂肪、淀粉动态累积量之间呈现极显著正相关关系(P<0.01)。【结论】驻玉216、驻玉902、驻玉927、郑单958、裕丰303春夏播玉米籽粒蛋白质、脂肪和淀粉动态累积与分配受环境影响较大,籽粒蛋白质、脂肪和淀粉动态累积量互为极显著正相关。

益气固表丸含药血清对香烟烟雾提取物诱导的3T3-L1前脂肪细胞脂质积累及炎症的影响研究

目的:通过体外实验观察益气固表丸含药血清(YQGB)对香烟烟雾提取物(CSE)诱导的3T3-L1前脂肪细胞脂质积累及炎症的影响。方法:诱导3T3-L1前脂肪细胞分化,油红O染色及试剂盒检测分析细胞内脂质积累水平,使用CCK-8检测分化过程中不同浓度CSE、终浓度为CSE 2%及不同浓度的益气固表丸含药血清、过氧化物酶体增殖物激活受体γ(PPARγ)激动剂(罗格列酮,ROS)对细胞增殖能力的影响;采用Western blot和RT-qPCR法测定PPARγ、CCAAT增强子结合蛋白α(C/EBPα)、甾醇调节元件结合蛋白-1c(SREBP-1c)、白细胞介素-6(IL-6)、肿瘤坏死因子α(TNF-α)的蛋白及mRNA的表达量。采用ELISA法检测细胞培养上清IL-6、TNF-α的表达量。结果:油红O染色显示,3T3-L1前体脂肪细胞10 d后已诱导为完全成熟的脂肪细胞;与对照组相比,CSE组油红O染色出现明显的脂滴积累,并且总胆固醇(TC)和甘油三酯(TG)含量明显增加,益气固表丸含药血清可以降低TC、TG的含量,且其抑制作用呈浓度依赖性(P<0.05)。Western blot和RT-qPCR结果显示,益气固表丸含药血清以浓度依赖的方式降低PPARγ、C/EBPα、SREBP-1c、IL-6、TNF-α蛋白及mRNA表达(P<0.05)。ELISA检测显示,益气固表丸含药血清可降低IL-6、TNF-α表达,且其抑制作用呈浓度依赖性(P<0.05)。结论:益气固表丸含药血清可以缓解CSE诱导3T3-L1脂肪细胞的脂质积累,抑制细胞炎症因子分泌,发挥抑炎作用。

脂肪抽吸联合腹壁成形术对腹部脂肪堆积伴腹壁松弛患者腹部形态及并发症的影响

目的 探讨脂肪抽吸联合腹壁成形术对腹部脂肪堆积伴腹壁松弛患者腹部形态及并发症的影响。方法 选取2020年 1月至2023年12月在贵州整形口腔美容外科医院接受治疗的腹部脂肪堆积伴腹壁松弛患者78例,分为对照组和观察组,各39例。对照组患者仅接受脂肪抽吸术,观察组患者接受脂肪抽吸联合腹壁成形术。比较两组患者术前、术后3个月的腹部周径、皮褶厚度、腹壁松弛度、腹壁功能恢复评分以及术后并发症发生情况和患者满意度。结果 术后3个月,观察组患者腹部周径、皮褶厚度、腹壁松弛度均低于对照组,差异有统计学意义(P<0.05)。观察组患者并发症发生率为5.1%(2/39),低于对照组17.9%(7/39),但差异无统计学意义(P>0.05)。观察组患者腹壁功能恢复评分(87.3±6.5)分,高于对照组(78.9±7.2)分,差异有统计学意义(P<0.05)。观察组患者满意度94.9%(37/39),高于对照组76.9%(30/39),差异有统计学意义(P<0.05)。结论 脂肪抽吸联合腹壁成形术能有效改善腹部脂肪堆积伴腹壁松弛患者的腹部形态,降低术后并发症发生率,促进腹壁功能恢复,提高患者满意度,值得临床推广应用。