A high performance liquid chromatographic method for the simultaneous determination of four fat soluble vitamins: retinol (vitamin A), cholecalciferol(vitamin D), tocopherol(vitamin E) and phylloquinone(vitamin K_1) i...A high performance liquid chromatographic method for the simultaneous determination of four fat soluble vitamins: retinol (vitamin A), cholecalciferol(vitamin D), tocopherol(vitamin E) and phylloquinone(vitamin K_1) in fortified milk powders and egg yolk has been developed. The method requires enzymatic hydrolysis of lipid component of the sample as a pretreatment. Several factors which influence the enzymatic hydrolysis were studied-Separation was achieved using μ-Bondapak C-18 column(3.9×300mm), 98% methanol as mobile phase, a double channel detection was selected; vitamins D_3 E_1 K_1 were detected by UV spectrophotometry (265 nm) first, then vitamin A by fluorometry (EX 325nm, EM 480mm). The retention times of vitamin A_1 D_3, E and K_1 4.87, 9.00, 10.58 and 15.45 min respectively. Detection limit were 0.64, 0.25, 0.50 and 0.07 ng; and the recoveries were 90.5%～103.6% 90.0%～95.6%, 91.7%～98.8%, 91.5%～98.6%, respectively. The vitamins A, D_0, E, K_1 contents in foods were determined satisfactorily.展开更多
The aim of this study was to determine the plasma levels of vitamin K-dependent protein growth arrest-specific protein 6 (Gas6) and its soluble receptor Axl (sAxl) in patients with hepatocellular carcinoma (HCC), acut...The aim of this study was to determine the plasma levels of vitamin K-dependent protein growth arrest-specific protein 6 (Gas6) and its soluble receptor Axl (sAxl) in patients with hepatocellular carcinoma (HCC), acute hepatitis (AH), fulminant hepatitis(FH), chronic hepatitis (CH), and liver cirrhosis (LC) and to determine whether Gas6 and sAxl can be used as biomarkers. Immunoassys were used to measure levels of plasma Gas6 and sAxl in 40 patients with HCC, 13 patients with AH, 3 patients with FH, 7 patients with LC, and 20 healthy normal adult controls (NC). Furthermore, urinary γ-carboxyglutamic acid (Gla) was measured by high performance liquid chromatography. We have addressed this issue by conducting a cross-sectional study to determine whether plasma Gas6 and sAxl levels are associated with DCP, urinary γ-Gla, and liver functions in humans. Levels of Gas6, sAxl, and γ-Gla were significantly higher in HCC as compared to those in NC, and they were significantly positive correlated. Gas6/sAxl molar ratios in HCC were significantly higher than in NC, but those ratios in AH, FH, and LC were significantly lower in NC. Furthermore, Gas6/sAxl molar ratios in HCC increased significantly in comparison with those AH and LC. The increase of Gas6, sAxl and Gas6/sAxl molar ratio were correlated with the progression and poor prognosis of HCC. Thus, Gas6 and sAxl may be useful biomarkers for HCC.展开更多
目的评估自动化磁珠法提取血清脂溶性维生素应用液相色谱串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS/MS)检测的性能。方法收集200例临床剩余血清样本,采用自动化磁珠法提取血清中脂溶性维生素A,D_(2),D_(3),E和K...目的评估自动化磁珠法提取血清脂溶性维生素应用液相色谱串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS/MS)检测的性能。方法收集200例临床剩余血清样本,采用自动化磁珠法提取血清中脂溶性维生素A,D_(2),D_(3),E和K;同时联合LC-MS/MS检测脂溶性维生素A,D_(2),D_(3),E和K的线性、定量限、精密度、正确度、携带污染率等性能指标以及基质效应。并比较此方法与传统萃取法检测结果的一致性。结果自动化磁珠法提取脂溶性维生素A,D_(2),D_(3),E和K线性相关系数均>0.99;五种物质的定量限分别为5,0.25,0.25,125和0.025ng/ml;批内精密度和批间精密度分别为0.66%~4.83%,0.15%~3.70%;平均加标回收率为87.05%~111.11%;基质效应为95.43%~99.07%;高-低值样本循环进样结果均值与低-低值样本循环进样结果均值之差,均小于低-低值样本循环进样结果均值的3s;统计学结果显示自动化磁珠法和传统萃取法提取的脂溶性维生素结果相关性良好(r>0.99),两种方法的检测结果无显著偏倚。结论自动化磁珠法提取脂溶性维生素的检测性能良好,有望提高样品通量和分析效率。展开更多
选用3个藜麦品种(晋藜1号、晋藜2号、晋藜3号),在拔节期进行叶面喷施0%(CK)、0.1%(B_(1))、0.2%(B_(2))、0.3%(B_(3))的硼酸溶液50 mL 2次,研究硼对藜麦叶片营养成分的影响。结果表明:藜麦不同生长阶段叶片可溶性蛋白、可溶性糖、维生素...选用3个藜麦品种(晋藜1号、晋藜2号、晋藜3号),在拔节期进行叶面喷施0%(CK)、0.1%(B_(1))、0.2%(B_(2))、0.3%(B_(3))的硼酸溶液50 mL 2次,研究硼对藜麦叶片营养成分的影响。结果表明:藜麦不同生长阶段叶片可溶性蛋白、可溶性糖、维生素E含量不同,不同浓度硼对藜麦叶片这些成分有明显影响。随着硼浓度的增加,3种藜麦叶片可溶性蛋白含量逐渐增加,在B_(3)达到最高;成熟期3种藜麦叶片可溶性蛋白含量最高,B_(3)处理较CK分别增加49.28%、43.77%、17.35%。适宜浓度的硼可提高藜麦叶片可溶性糖含量,随着硼浓度的增加呈先增高后降低趋势,在B_(2)时最高;灌浆期3种藜麦叶片可溶性糖含量最高,B_(2)的可溶性糖含量较CK分别增加16.24%、38.18%、41.52%。施用硼肥后,3种藜麦叶片维生素E含量较不施硼处理均有所提高;孕穗期藜麦叶片维生素E含量最高,晋藜1号叶片维生素E含量最高时为B_(3)处理,较CK增长39%,晋藜2号、晋藜3号叶片维生素E含量最高时为B_(2)处理,分别较CK增加24%、37%。B_(1)、B_(2)、B_(3)处理藜麦叶片可溶性蛋白、可溶性糖、维生素E含量均高于CK。实际生产中,选用0.3%的硼最为合适,根据需要选择不同生长期藜麦叶片。展开更多
文摘A high performance liquid chromatographic method for the simultaneous determination of four fat soluble vitamins: retinol (vitamin A), cholecalciferol(vitamin D), tocopherol(vitamin E) and phylloquinone(vitamin K_1) in fortified milk powders and egg yolk has been developed. The method requires enzymatic hydrolysis of lipid component of the sample as a pretreatment. Several factors which influence the enzymatic hydrolysis were studied-Separation was achieved using μ-Bondapak C-18 column(3.9×300mm), 98% methanol as mobile phase, a double channel detection was selected; vitamins D_3 E_1 K_1 were detected by UV spectrophotometry (265 nm) first, then vitamin A by fluorometry (EX 325nm, EM 480mm). The retention times of vitamin A_1 D_3, E and K_1 4.87, 9.00, 10.58 and 15.45 min respectively. Detection limit were 0.64, 0.25, 0.50 and 0.07 ng; and the recoveries were 90.5%～103.6% 90.0%～95.6%, 91.7%～98.8%, 91.5%～98.6%, respectively. The vitamins A, D_0, E, K_1 contents in foods were determined satisfactorily.
文摘The aim of this study was to determine the plasma levels of vitamin K-dependent protein growth arrest-specific protein 6 (Gas6) and its soluble receptor Axl (sAxl) in patients with hepatocellular carcinoma (HCC), acute hepatitis (AH), fulminant hepatitis(FH), chronic hepatitis (CH), and liver cirrhosis (LC) and to determine whether Gas6 and sAxl can be used as biomarkers. Immunoassys were used to measure levels of plasma Gas6 and sAxl in 40 patients with HCC, 13 patients with AH, 3 patients with FH, 7 patients with LC, and 20 healthy normal adult controls (NC). Furthermore, urinary γ-carboxyglutamic acid (Gla) was measured by high performance liquid chromatography. We have addressed this issue by conducting a cross-sectional study to determine whether plasma Gas6 and sAxl levels are associated with DCP, urinary γ-Gla, and liver functions in humans. Levels of Gas6, sAxl, and γ-Gla were significantly higher in HCC as compared to those in NC, and they were significantly positive correlated. Gas6/sAxl molar ratios in HCC were significantly higher than in NC, but those ratios in AH, FH, and LC were significantly lower in NC. Furthermore, Gas6/sAxl molar ratios in HCC increased significantly in comparison with those AH and LC. The increase of Gas6, sAxl and Gas6/sAxl molar ratio were correlated with the progression and poor prognosis of HCC. Thus, Gas6 and sAxl may be useful biomarkers for HCC.
文摘目的评估自动化磁珠法提取血清脂溶性维生素应用液相色谱串联质谱法(liquid chromatography-tandem mass spectrometry,LC-MS/MS)检测的性能。方法收集200例临床剩余血清样本,采用自动化磁珠法提取血清中脂溶性维生素A,D_(2),D_(3),E和K;同时联合LC-MS/MS检测脂溶性维生素A,D_(2),D_(3),E和K的线性、定量限、精密度、正确度、携带污染率等性能指标以及基质效应。并比较此方法与传统萃取法检测结果的一致性。结果自动化磁珠法提取脂溶性维生素A,D_(2),D_(3),E和K线性相关系数均>0.99;五种物质的定量限分别为5,0.25,0.25,125和0.025ng/ml;批内精密度和批间精密度分别为0.66%~4.83%,0.15%~3.70%;平均加标回收率为87.05%~111.11%;基质效应为95.43%~99.07%;高-低值样本循环进样结果均值与低-低值样本循环进样结果均值之差,均小于低-低值样本循环进样结果均值的3s;统计学结果显示自动化磁珠法和传统萃取法提取的脂溶性维生素结果相关性良好(r>0.99),两种方法的检测结果无显著偏倚。结论自动化磁珠法提取脂溶性维生素的检测性能良好,有望提高样品通量和分析效率。
文摘选用3个藜麦品种(晋藜1号、晋藜2号、晋藜3号),在拔节期进行叶面喷施0%(CK)、0.1%(B_(1))、0.2%(B_(2))、0.3%(B_(3))的硼酸溶液50 mL 2次,研究硼对藜麦叶片营养成分的影响。结果表明:藜麦不同生长阶段叶片可溶性蛋白、可溶性糖、维生素E含量不同,不同浓度硼对藜麦叶片这些成分有明显影响。随着硼浓度的增加,3种藜麦叶片可溶性蛋白含量逐渐增加,在B_(3)达到最高;成熟期3种藜麦叶片可溶性蛋白含量最高,B_(3)处理较CK分别增加49.28%、43.77%、17.35%。适宜浓度的硼可提高藜麦叶片可溶性糖含量,随着硼浓度的增加呈先增高后降低趋势,在B_(2)时最高;灌浆期3种藜麦叶片可溶性糖含量最高,B_(2)的可溶性糖含量较CK分别增加16.24%、38.18%、41.52%。施用硼肥后,3种藜麦叶片维生素E含量较不施硼处理均有所提高;孕穗期藜麦叶片维生素E含量最高,晋藜1号叶片维生素E含量最高时为B_(3)处理,较CK增长39%,晋藜2号、晋藜3号叶片维生素E含量最高时为B_(2)处理,分别较CK增加24%、37%。B_(1)、B_(2)、B_(3)处理藜麦叶片可溶性蛋白、可溶性糖、维生素E含量均高于CK。实际生产中,选用0.3%的硼最为合适,根据需要选择不同生长期藜麦叶片。
