To investigate the distribution of variant genotypes of Fc gamma receptor Ⅲa (FcγRⅢa) in healthy Chinese population of Zhengzhou city, genomic DNA was extracted from peripheral blood of healthy donators The genot...To investigate the distribution of variant genotypes of Fc gamma receptor Ⅲa (FcγRⅢa) in healthy Chinese population of Zhengzhou city, genomic DNA was extracted from peripheral blood of healthy donators The genotypes of FcγRⅢa-158 were determined by nested polymerase chain reaction (PCR) in 137 healthy people in Zhengzhou city The results showed that frequencies of variant genotypes FF, VV and VF were 42 3 %, 48 9 % and 8 8 % respectively The distribution of FcγRⅢa-158 in healthy Chinese population of Zhengzhou city was polymorphic and different from that of African Americans (AA) and Caucasian Americans (CA)展开更多
Objective To explore the roles of Fcγ recep tor in the pathogenesis of Graves' disease. Methods Fcγ receptor gene knockout mice(Fcγ R KO m ice) which were rooted in C57BL/6 mice and wild type C57BL/6 mice wer...Objective To explore the roles of Fcγ recep tor in the pathogenesis of Graves' disease. Methods Fcγ receptor gene knockout mice(Fcγ R KO m ice) which were rooted in C57BL/6 mice and wild type C57BL/6 mice were immunized by hTSH receptor expressing cells (DAP3.WT). 1-2×107 DAP3.WT cells were peri toneally injected into mice every two weeks for a total of six times. Two weeks after final immunization, mice were killed for measurement of total thyroxine, T RAb and pathological examination. Results The thyroxine level of the immunized Fcγ recept or gene knockout mice was significantly lower than that of the immunized wild ty pe control mice (2.2±0.31 vs. 3.32± 0.59 g·dL -1, P< 0.05 ),but there was no significant difference between immunized Fcγ R KO mic e and non-immunized wild type control group. The TRAb levels of the immunized F γ R KO mice significantly increased compared to those of the immunized wild type mice (21.75±8.21 vs. 14.11±6.21, P< 0.05). The lymphocyte cel ls infiltration and destruction of thyroid follicles were found in the thyroid gland of the immunized Fcγ R KO mice. Conclusion These results suggest that Fcγ receptor may be involved in the pathogenesis of Graves' disease.展开更多
IgA Fc受体(FcαRI,CD89)属于免疫受体家族成员,主要表达于单核-巨噬细胞、中性粒细胞、嗜酸性粒细胞和树突状细胞等免疫效应细胞表面。FcαRI能特异的与血清型和分泌型IgA结合,并通过γ链介导一系列免疫反应,包括抗体依赖细胞介导的细...IgA Fc受体(FcαRI,CD89)属于免疫受体家族成员,主要表达于单核-巨噬细胞、中性粒细胞、嗜酸性粒细胞和树突状细胞等免疫效应细胞表面。FcαRI能特异的与血清型和分泌型IgA结合,并通过γ链介导一系列免疫反应,包括抗体依赖细胞介导的细胞毒性作用(ADCC)、补体依赖的细胞毒性(CDC)及细胞吞噬作用等。FcαRI是一种双功能受体,在不同的生理条件下可以介导免疫系统的活化和抑制反应。FcαRI在机体免疫防御和在维持系统免疫平衡方面扮演着重要的角色,有望成为治疗人类疾病的新靶点。本文对FcαRI的结构、功能及其应用等研究现状进行综述。展开更多
应用两种不同的表达系统对FceR I α亚基的细胞外区进行克隆和表达,表达产物经纯化后用免疫斑点杂交法检测其与IgE结合的能力,探索IgE与其高亲和力受体FceR I α亚基的细胞外区结合的机制。结果两种体系均成功表达出FceRI α亚基的细胞...应用两种不同的表达系统对FceR I α亚基的细胞外区进行克隆和表达,表达产物经纯化后用免疫斑点杂交法检测其与IgE结合的能力,探索IgE与其高亲和力受体FceR I α亚基的细胞外区结合的机制。结果两种体系均成功表达出FceRI α亚基的细胞外区,pBAD/gⅢA表达的FceR I α亚基的细胞外区能与IgE结合,而PQE30表达的FeaR I α亚基的细胞外区不能与IgE结合。提示FccR I α亚基的细胞外区已足够和IgE结合,无需β、γ亚基的存在,其所具有的一定的空间构型和二硫键的形成在与IgE结合时是必需的,而糖基化位点在与IgE的结合时是非必需的。展开更多
文摘To investigate the distribution of variant genotypes of Fc gamma receptor Ⅲa (FcγRⅢa) in healthy Chinese population of Zhengzhou city, genomic DNA was extracted from peripheral blood of healthy donators The genotypes of FcγRⅢa-158 were determined by nested polymerase chain reaction (PCR) in 137 healthy people in Zhengzhou city The results showed that frequencies of variant genotypes FF, VV and VF were 42 3 %, 48 9 % and 8 8 % respectively The distribution of FcγRⅢa-158 in healthy Chinese population of Zhengzhou city was polymorphic and different from that of African Americans (AA) and Caucasian Americans (CA)
文摘Objective To explore the roles of Fcγ recep tor in the pathogenesis of Graves' disease. Methods Fcγ receptor gene knockout mice(Fcγ R KO m ice) which were rooted in C57BL/6 mice and wild type C57BL/6 mice were immunized by hTSH receptor expressing cells (DAP3.WT). 1-2×107 DAP3.WT cells were peri toneally injected into mice every two weeks for a total of six times. Two weeks after final immunization, mice were killed for measurement of total thyroxine, T RAb and pathological examination. Results The thyroxine level of the immunized Fcγ recept or gene knockout mice was significantly lower than that of the immunized wild ty pe control mice (2.2±0.31 vs. 3.32± 0.59 g·dL -1, P< 0.05 ),but there was no significant difference between immunized Fcγ R KO mic e and non-immunized wild type control group. The TRAb levels of the immunized F γ R KO mice significantly increased compared to those of the immunized wild type mice (21.75±8.21 vs. 14.11±6.21, P< 0.05). The lymphocyte cel ls infiltration and destruction of thyroid follicles were found in the thyroid gland of the immunized Fcγ R KO mice. Conclusion These results suggest that Fcγ receptor may be involved in the pathogenesis of Graves' disease.
文摘应用两种不同的表达系统对FceR I α亚基的细胞外区进行克隆和表达,表达产物经纯化后用免疫斑点杂交法检测其与IgE结合的能力,探索IgE与其高亲和力受体FceR I α亚基的细胞外区结合的机制。结果两种体系均成功表达出FceRI α亚基的细胞外区,pBAD/gⅢA表达的FceR I α亚基的细胞外区能与IgE结合,而PQE30表达的FeaR I α亚基的细胞外区不能与IgE结合。提示FccR I α亚基的细胞外区已足够和IgE结合,无需β、γ亚基的存在,其所具有的一定的空间构型和二硫键的形成在与IgE结合时是必需的,而糖基化位点在与IgE的结合时是非必需的。
