The feminization-1c(fem-1c)gene has been shown to be associated with sex differentiation and determination in many metazoan species.It belongs to the fem-1 family which is a member of the ANK superfamily.In this study...The feminization-1c(fem-1c)gene has been shown to be associated with sex differentiation and determination in many metazoan species.It belongs to the fem-1 family which is a member of the ANK superfamily.In this study,the full-length cDNA of the fem-1c(Hcfem-1c)gene was isolated from the freshwater mussel(Hypriopsis cumingii).The isolated Hcfem-1c cDNA was 2196 bp in length and encoded a putative protein of 621 amino acids that contains seven ANK domains.Phylogenetic analysis of the deduced HcFEM-1C protein showed that it clustered with the other invertebrates homologues,indicating that the sequence of HcFEM-1C was conserved during evolution.Quantitative real-time PCR(qPCR)expression revealed that the Hcfem-1c gene was expressed in the adductor muscle,foot,liver,gill,kidney,mantle,and gonads of male and female adult mussels(two years old).In the gonads Hcfem-1c was much less abundant in males than that in females.During early development of the gonads,Hcfem-1c transcripts were significantly increased in the primordial germ cell differentiation stage(5 months old).We hypothesized that Hcfem-1c probably regulates female gonad differentiation.In situ hybridization showed that a strong and specific signal concentrated in the female oocyte cell membrane and male follicular wall,indicating that Hcfem-1c gene may not only be involved in female gonad differentiation,but also participates in egg development.This study laid the foundations for a better understanding of gender differentiation mechanism in H.cumingii.展开更多
基金This work was supported by the National Natural Science Foundation of China[grant number 31772835]the Innovation Program of Shanghai Municipal Education Commission[grant number 13ZZ128]the Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding[grant number ZF1206].
文摘The feminization-1c(fem-1c)gene has been shown to be associated with sex differentiation and determination in many metazoan species.It belongs to the fem-1 family which is a member of the ANK superfamily.In this study,the full-length cDNA of the fem-1c(Hcfem-1c)gene was isolated from the freshwater mussel(Hypriopsis cumingii).The isolated Hcfem-1c cDNA was 2196 bp in length and encoded a putative protein of 621 amino acids that contains seven ANK domains.Phylogenetic analysis of the deduced HcFEM-1C protein showed that it clustered with the other invertebrates homologues,indicating that the sequence of HcFEM-1C was conserved during evolution.Quantitative real-time PCR(qPCR)expression revealed that the Hcfem-1c gene was expressed in the adductor muscle,foot,liver,gill,kidney,mantle,and gonads of male and female adult mussels(two years old).In the gonads Hcfem-1c was much less abundant in males than that in females.During early development of the gonads,Hcfem-1c transcripts were significantly increased in the primordial germ cell differentiation stage(5 months old).We hypothesized that Hcfem-1c probably regulates female gonad differentiation.In situ hybridization showed that a strong and specific signal concentrated in the female oocyte cell membrane and male follicular wall,indicating that Hcfem-1c gene may not only be involved in female gonad differentiation,but also participates in egg development.This study laid the foundations for a better understanding of gender differentiation mechanism in H.cumingii.