Using microalgae( SA),artificial feed( AF),Brachionus plicatilis( BP) and brine shrimp larvae( BS),four diets including SA + AF,SA + AF + BP,SA + AF + BS and SA + AF + BP + BS were formulated and used to rear Fennerop...Using microalgae( SA),artificial feed( AF),Brachionus plicatilis( BP) and brine shrimp larvae( BS),four diets including SA + AF,SA + AF + BP,SA + AF + BS and SA + AF + BP + BS were formulated and used to rear Fenneropenaeus chinensis larvae. The fertilized eggs fed with SA + AF were divided into disinfection group with iodophor and untreated group,and the fertilized eggs fed with other diets were all disinfected with iodophor. The survival rates,body weight and WSSV load of post larvae at each stage were compared. The results showed that there was no significant difference in the survival rate of prawns at the N-Z stage by feeding with different diets( P > 0. 05),but the survival rate of prawns fed with BP was higher than others( P < 0. 01) at the Z-P stage. The body weight of post larvae( P10) fed with SA + AF + BP was less than that fed with SA + AF + BP + BS( P <0. 01). The WSSV load of prawns( 10. 52 ±3. 3 copies/ng DNA) fed with SA + AF were significantly lower than that of other treatments( P < 0. 05) at the P10 stage. During the P11- P60 stage,four kinds of diets,including Ruditapes philippinarum foot muscle( CF) + artificial feed( AF),R. philippinarum foot muscle radiated by60 Co γ-ray( RCF) + artificial feed( AF),potassium permanganate-disinfected R. philippinarum foot muscle( DCF) + artificial feed( AF) and artificial feed( AF),were used to feed prawns. The prawns fed with CF + AF gained the greatest increase of body weight and body length,but had no significant difference with that fed with AF( P > 0. 05); prawns fed with CF + AF and AF had significant difference with the other two groups( P < 0. 05). The survival rates of prawns fed with four different diets had no significant difference with each other( P > 0. 05). WSSV artificial infection test showed that the accumulated mortality of prawns fed with four diets were higher than 90%,and the difference was not significant( P > 0. 05).展开更多
In this study,families of selected population for growth(SP_BWT),selected population for white spot syndrome virus(WSSV)resistance(SP_RW),Bohai wild population(WP_BH)and Huanghai wild population(WP_HH)of F.chinensis w...In this study,families of selected population for growth(SP_BWT),selected population for white spot syndrome virus(WSSV)resistance(SP_RW),Bohai wild population(WP_BH)and Huanghai wild population(WP_HH)of F.chinensis were constructed through artificial insemination and with the standardized procedure of larvae rearing.Growth and survival performance were studied among four populations after a 70 days common test.The results showed that the maximum least square mean of body weight was 17.50 g in SP_BWT while the minimum was 13.03 g in WP_HH.Compared with WP_BH,body weight of SP_BWT increased by 23.41%(P<0.01)and that of SP_RW by 12.20%(P>0.05).Body weights of SP_BWT and SP_RW were significantly higher than that of WP_HH,which increased by 34.31%(P<0.01)and 22.10%(P<0.05),respectively.The mean AGR of four populations was 0.19,0.18,0.17 and 0.16 g d^(-1),respectively.Coefficient of variation of body weight among four populations was high,which ranged from 32.67% to 35.25%.Such a range showed that there was the potentiality for further improvement in selected populations.Coefficient of variation of survival rate among four populations was low,varying between 3.20% and 5.90%.The difference of survival was highly significant(P<0.01)between SP_BWT and WP_BH,and significant(P<0.05)between SP_RW and WP_BH.However,no significant difference among other populations(P>0.05)was observed.Different growth performances were also observed among different families in each population.The body weight of 798F family was the highest.The absolute growth rate(AGR)was 0.25 g d^(-1),150%higher than that of the lowest one,0.1 g d^(-1) in 807F family.Survival rate of families among four populations was different.The highest was 94.74%,and the lowest was 71.88%.展开更多
基金Supported by National 863 Project(2012AA10A404)General Program of National Natural Science Foundation of China(31372523)Taishan Scholar Program for Seed Industry
文摘Using microalgae( SA),artificial feed( AF),Brachionus plicatilis( BP) and brine shrimp larvae( BS),four diets including SA + AF,SA + AF + BP,SA + AF + BS and SA + AF + BP + BS were formulated and used to rear Fenneropenaeus chinensis larvae. The fertilized eggs fed with SA + AF were divided into disinfection group with iodophor and untreated group,and the fertilized eggs fed with other diets were all disinfected with iodophor. The survival rates,body weight and WSSV load of post larvae at each stage were compared. The results showed that there was no significant difference in the survival rate of prawns at the N-Z stage by feeding with different diets( P > 0. 05),but the survival rate of prawns fed with BP was higher than others( P < 0. 01) at the Z-P stage. The body weight of post larvae( P10) fed with SA + AF + BP was less than that fed with SA + AF + BP + BS( P <0. 01). The WSSV load of prawns( 10. 52 ±3. 3 copies/ng DNA) fed with SA + AF were significantly lower than that of other treatments( P < 0. 05) at the P10 stage. During the P11- P60 stage,four kinds of diets,including Ruditapes philippinarum foot muscle( CF) + artificial feed( AF),R. philippinarum foot muscle radiated by60 Co γ-ray( RCF) + artificial feed( AF),potassium permanganate-disinfected R. philippinarum foot muscle( DCF) + artificial feed( AF) and artificial feed( AF),were used to feed prawns. The prawns fed with CF + AF gained the greatest increase of body weight and body length,but had no significant difference with that fed with AF( P > 0. 05); prawns fed with CF + AF and AF had significant difference with the other two groups( P < 0. 05). The survival rates of prawns fed with four different diets had no significant difference with each other( P > 0. 05). WSSV artificial infection test showed that the accumulated mortality of prawns fed with four diets were higher than 90%,and the difference was not significant( P > 0. 05).
基金supported by the National Natural Science Foundation of China (Nos.31372523 and 31302180)the Scientific and Technological Innovation Project Financially Supported by Qingdao National Laboratory for Marine Science and Technology (No.2015ASKJ02)+4 种基金the Taishan Scholar Program for Seed Industrythe Shandong Provincial Natural Science Foundation (No.ZR2014CQ001)the Special Fund for Postdoctoral Innovative Project of Shandong ProvinceCentral Public-Interest Scientific Institution Basal Research Fund of the Yellow Sea Fisheries Research Institute (No.20603022015013)the China Agriculture Research System (CARS-48)
文摘In this study,families of selected population for growth(SP_BWT),selected population for white spot syndrome virus(WSSV)resistance(SP_RW),Bohai wild population(WP_BH)and Huanghai wild population(WP_HH)of F.chinensis were constructed through artificial insemination and with the standardized procedure of larvae rearing.Growth and survival performance were studied among four populations after a 70 days common test.The results showed that the maximum least square mean of body weight was 17.50 g in SP_BWT while the minimum was 13.03 g in WP_HH.Compared with WP_BH,body weight of SP_BWT increased by 23.41%(P<0.01)and that of SP_RW by 12.20%(P>0.05).Body weights of SP_BWT and SP_RW were significantly higher than that of WP_HH,which increased by 34.31%(P<0.01)and 22.10%(P<0.05),respectively.The mean AGR of four populations was 0.19,0.18,0.17 and 0.16 g d^(-1),respectively.Coefficient of variation of body weight among four populations was high,which ranged from 32.67% to 35.25%.Such a range showed that there was the potentiality for further improvement in selected populations.Coefficient of variation of survival rate among four populations was low,varying between 3.20% and 5.90%.The difference of survival was highly significant(P<0.01)between SP_BWT and WP_BH,and significant(P<0.05)between SP_RW and WP_BH.However,no significant difference among other populations(P>0.05)was observed.Different growth performances were also observed among different families in each population.The body weight of 798F family was the highest.The absolute growth rate(AGR)was 0.25 g d^(-1),150%higher than that of the lowest one,0.1 g d^(-1) in 807F family.Survival rate of families among four populations was different.The highest was 94.74%,and the lowest was 71.88%.