An optical fiber bienzyme sensor based on the luminol chemiluminescent reaction was developed and demonstrated to be sensitive to glucose. Glucose oxidase(GOD) and horseradish peroxidase(HRP) were co-immobilized by mi...An optical fiber bienzyme sensor based on the luminol chemiluminescent reaction was developed and demonstrated to be sensitive to glucose. Glucose oxidase(GOD) and horseradish peroxidase(HRP) were co-immobilized by microencapsulation in a sol-gel film derived from tetraethyl orthosilicate(TEOS). The calibration plots for glucose were established by the optical fiber glucose sensor fabricated by attaching the bienzyme silica gel onto the glass window of the fiber bundle. The linear range was 0 2-2 mmol/L and the detection limit was approximately 0 12 mmol/L. The relative standard deviation was 5.3% ( n =6). The proposed biosensor was applied to glucose assay in ofloxacin injection successfully.展开更多
Luciferase from firefly lantern extract was immobilized on CNBr activated Sepharose 4B. The kinetic properties of immobilized luciferase were extensively studied. The K m′ for D luciferin is 11.9 μmol/L, the ...Luciferase from firefly lantern extract was immobilized on CNBr activated Sepharose 4B. The kinetic properties of immobilized luciferase were extensively studied. The K m′ for D luciferin is 11.9 μmol/L, the optimum pH and temperature for Sepharose bound enzyme were 7.8 and 25℃ respectively. A luminescence fiber optic biosensor, making use of immobilized crude luciferase, was developed for assay of ATP. The peak light intensity was linear with respect to ATP concentration in range of 10 -9 -10 -5 mol/L. A biological application was also demonstrated with the determination of serum ATP from rats bred in low versus normal oxygen environments.展开更多
Fiber-optic DNA biosensors are a kind of ana-lytic setups, which convert the Waston-Crick base pairs matching duplex or Hoogsteen’s tri-plex (T/A-T, C/G-C) formation into a readable analytical signals when functional...Fiber-optic DNA biosensors are a kind of ana-lytic setups, which convert the Waston-Crick base pairs matching duplex or Hoogsteen’s tri-plex (T/A-T, C/G-C) formation into a readable analytical signals when functionalized single- strands DNA (ssDNA) or double-strands DNA (dsDNA) of interest are immobilized on the sur-face of fiber-optic hybrids with target DNA or interacts with ligands. This review will provide the information about the fiber-optic DNA bio-sensors classified into two categories depend-ing on the end fiber and side fiber with or with-out the labels—label-free fiber-optic DNA bio-sensors and labeled fiber-optic DNA biosensor in recent years. Both are dissertated, and em-phasis is on the label-free fiber-optic DNA bio-sensors. Fiber-optic DNA biosensors had got great progresses because fiber-optic has more advantages over the other transducers and are easily processed by nanotechnology. So fiber- optic DNA biosensors have increasingly at-tracted more attention to research and develop the new fiber-optic DNA biosensors that inte-grated with the “nano-bio-info” technology for in vivo test, single molecular detection and on-line medical diagnosis. Finally, future pros-pects to the fiber-optic DNA biosensors are predicted.展开更多
A surface plasmon resonance(SPR) sensor with two orthogonal open loops based on microstructured optical fibers(MOFs) is introduced. The interaction between core mode and surface plasmon polariton(SPP) mode produced by...A surface plasmon resonance(SPR) sensor with two orthogonal open loops based on microstructured optical fibers(MOFs) is introduced. The interaction between core mode and surface plasmon polariton(SPP) mode produced by two different metal films is studied. Full vector finite element method is used to analyze the coupling and sensing characteristics.The results show that there are three loss peaks near the Au/Ag film, and multi-peak calibration is achieved. Because of the positive and negative sensitivity of the amplitude, the sensor has strong anti-interference capability when the external environment changes. The sensor can detect the refractive index between 1.37 and 1.40, and the working wavelength is between 1600 nm and 2400 nm. Because the sensor has some excellent characteristics, it can be used in biochemical sensing, environmental detection, and other related fields.展开更多
文摘An optical fiber bienzyme sensor based on the luminol chemiluminescent reaction was developed and demonstrated to be sensitive to glucose. Glucose oxidase(GOD) and horseradish peroxidase(HRP) were co-immobilized by microencapsulation in a sol-gel film derived from tetraethyl orthosilicate(TEOS). The calibration plots for glucose were established by the optical fiber glucose sensor fabricated by attaching the bienzyme silica gel onto the glass window of the fiber bundle. The linear range was 0 2-2 mmol/L and the detection limit was approximately 0 12 mmol/L. The relative standard deviation was 5.3% ( n =6). The proposed biosensor was applied to glucose assay in ofloxacin injection successfully.
文摘Luciferase from firefly lantern extract was immobilized on CNBr activated Sepharose 4B. The kinetic properties of immobilized luciferase were extensively studied. The K m′ for D luciferin is 11.9 μmol/L, the optimum pH and temperature for Sepharose bound enzyme were 7.8 and 25℃ respectively. A luminescence fiber optic biosensor, making use of immobilized crude luciferase, was developed for assay of ATP. The peak light intensity was linear with respect to ATP concentration in range of 10 -9 -10 -5 mol/L. A biological application was also demonstrated with the determination of serum ATP from rats bred in low versus normal oxygen environments.
文摘Fiber-optic DNA biosensors are a kind of ana-lytic setups, which convert the Waston-Crick base pairs matching duplex or Hoogsteen’s tri-plex (T/A-T, C/G-C) formation into a readable analytical signals when functionalized single- strands DNA (ssDNA) or double-strands DNA (dsDNA) of interest are immobilized on the sur-face of fiber-optic hybrids with target DNA or interacts with ligands. This review will provide the information about the fiber-optic DNA bio-sensors classified into two categories depend-ing on the end fiber and side fiber with or with-out the labels—label-free fiber-optic DNA bio-sensors and labeled fiber-optic DNA biosensor in recent years. Both are dissertated, and em-phasis is on the label-free fiber-optic DNA bio-sensors. Fiber-optic DNA biosensors had got great progresses because fiber-optic has more advantages over the other transducers and are easily processed by nanotechnology. So fiber- optic DNA biosensors have increasingly at-tracted more attention to research and develop the new fiber-optic DNA biosensors that inte-grated with the “nano-bio-info” technology for in vivo test, single molecular detection and on-line medical diagnosis. Finally, future pros-pects to the fiber-optic DNA biosensors are predicted.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.61575066,61527822,and 61735005)the Natural Science Foundation of Guangdong Province,China(Grant No.2017A030313333)+4 种基金the Science and Technology Program of Guangzhou City,China(Grant No.201707010133)the Science and Technology Planning Project of Guangdong Province,China(Grant No.2017KZ010201)the GDUPS(2017)the Innovation Project of Graduate School of South China Normal University(Grant No.2018LKXM040)the SCNU Study Abroad Program for Elite Postgraduate Students,China
文摘A surface plasmon resonance(SPR) sensor with two orthogonal open loops based on microstructured optical fibers(MOFs) is introduced. The interaction between core mode and surface plasmon polariton(SPP) mode produced by two different metal films is studied. Full vector finite element method is used to analyze the coupling and sensing characteristics.The results show that there are three loss peaks near the Au/Ag film, and multi-peak calibration is achieved. Because of the positive and negative sensitivity of the amplitude, the sensor has strong anti-interference capability when the external environment changes. The sensor can detect the refractive index between 1.37 and 1.40, and the working wavelength is between 1600 nm and 2400 nm. Because the sensor has some excellent characteristics, it can be used in biochemical sensing, environmental detection, and other related fields.
