Morphological and radiological methods were used to study regeneration of the damaged bone of rat mandibles after application of plateletenriched fibrin clot. A bone hole was artificially created, and in the natural c...Morphological and radiological methods were used to study regeneration of the damaged bone of rat mandibles after application of plateletenriched fibrin clot. A bone hole was artificially created, and in the natural course of regeneration, the hole was immediately filled with blood and there a blood clot formed. After one week of healing, separate islands of young bone tissue appeared. After two to three weeks, the opening in the mandible was completely replaced by the young bone tissue. When a similar bone hole was filled with autological fibrin clot, the blood clot did not form. But after one week the entire hole was filed with newlyformed fused bone tissue. By the second week after the use of fibrin clot, the bone hole had further healed and bone callus was formed.展开更多
Cheonggukjang is a soybean paste made by fermenting whole cooked soybeans with Bacillus subtilis. Cheonggukjang contains a fibrinolytic enzyme that could provide clinical applications for removing blood clots. In the ...Cheonggukjang is a soybean paste made by fermenting whole cooked soybeans with Bacillus subtilis. Cheonggukjang contains a fibrinolytic enzyme that could provide clinical applications for removing blood clots. In the present study, the term ＂cheonggukjang kinase＂ （CGK） was used to refer to this fibrinolytic enzyme. The thrombolytic effects of CGK were analyzed in a rat model of cerebral embolic stroke produced by middle cerebral artery occlusion （MCAO）. Results from fibrin and platelet-rich clot lysis assays demonstrated that thrombolytic activity was greatest in CGKs, which were cultured for 40 hours. In addition, T50, the time needed to decompose 50% of the clot, did not change with plasminogen treatment, indicating that CGK was not a plasminogen activator, but was rather presumed to act as a plasmin-like protein. An intravenous infusion of CGK （1 U plasmin-like activity/100 μg CGK/kg） at 1 hour after MCAO resulted in removal of clots in a rat model of cerebral embolic stroke. CGK-treated groups exhibited a significant dose-dependent reduction in infarct volume. CGK treatment also improved functional recovery, as assessed by neurological deficit scores. Decreased infarct volume and improved functional recovery following CGK treatment was greater compared with recombinant tissue plasminogen activator （10 mg/kg）. These results suggested that CGK effectively reduced infarct volume and improved functional recovery following ischemic brain injury. CGK exhibits a number of potential clinical applications ir the treatment of cerebral embolic stroke.展开更多
Background: Many surgical haemostatic agents are available which aim to reduce morbidity and mortality of bleeding, as well as associated costs. We used qualitative techniques to compare the effectiveness of two topic...Background: Many surgical haemostatic agents are available which aim to reduce morbidity and mortality of bleeding, as well as associated costs. We used qualitative techniques to compare the effectiveness of two topical gelatine-based haemostatic agents in a porcine liver trauma model. Methods: We compared the activity of Floseal? (with human or bovine thrombin), Surgiflo? and Surgiflo? plus 5000 IU bovine thrombin per 10 mL of product (n = 6 test sites/group). Different clinical scenarios were evaluated in two anaesthetised piglets;normal physiological conditions (normotension and normothermia), and a status of hypotension, hypothermia and haemodilution, requiring inotropic support. Laparotomy was performed, and five identical stab wounds were made on each liver lobe. Four of these wounds were randomly treated with one of the agents under investigation, while one wound was kept as an untreated control. Haemostasis was observed during and immediately following surgery. After euthanisation, wounds were examined (blinded for treatment) macroscopically, microscopically and using scanning electron microscopic evaluation. Results: Surgiflo? produced some degree of clotting in 1/3 applications in both piglets. Co-treatment with thrombin resulted in a minor improvement in performance in the animal with normal physiological conditions (some degree of clotting in all wounds);no improvement was seen in the animal in a status of hypotension, hypothermia and haemodilution. Floseal? induced clotting in all wounds for both piglets. Microscopic and scanning electron microscope examination of the stab wounds demonstrated that Floseal? created a stable, dense agglomerate of gelatine and fibrin, which firmly adhered to the adjacent liver tissue. In wounds treated with Surgiflo? or Surgiflo? plus thrombin, the gelatine contained more air bubbles, resulting in less fibrin inclusion in the clot and weaker adhesion of the clot to the liver tissue. Conclusion: Floseal? created a dense and stable blood clot, even in a piglet with hypotension, hypothermia and haemodilution.展开更多
目的评价牙髓血运重建时运用富血小板血浆(PRP)或富血小板纤维蛋白(PRF)同传统的血凝块(BC)作为支架哪种材料效果更佳。方法检索PubMed、Embase、Cochrane、Web of Science、中国期刊全文数据库(CNKI)和万方数字期刊全文数据库,检索时...目的评价牙髓血运重建时运用富血小板血浆(PRP)或富血小板纤维蛋白(PRF)同传统的血凝块(BC)作为支架哪种材料效果更佳。方法检索PubMed、Embase、Cochrane、Web of Science、中国期刊全文数据库(CNKI)和万方数字期刊全文数据库,检索时间截止到2020年8月。通过RevMan 5.3和stata 13.0软件对纳入的研究的临床治愈率、根长、根管壁厚度和根尖封闭的疗效进行Meta分析。结果当牙髓血运重建时分别运用PRP或PRF同BC在临床治愈率和根尖封闭率上均无差异,与BC相比,PRP和PRF的临床治愈率的相对危险度(RR)为0.99(0.87,1.12),95%的置信区间(CI)为0.95(0.86,1.04),根尖封闭率的RR为1.09(0.91,1.30)和95%CI为1.02(0.83,1.25)。对牙根长度或根管壁厚度增加率分析时发现PRP或PRF同BC之间无差异,与BC相比,PRP和PRF的牙根长度增加率的RR及95%CI分别为1.06(0.86,1.32)和1.10(0.90,1.35),根管壁厚度增加率的RR及95%CI分别为1.04(0.86,1.26)和1.09(0.76,1.57)。对牙根长度增加量分析时发现PRP优于BC,其加权均数差(WMD)及95%CI为1.47(0.57,2.36)mm。结论在行牙髓血运重建时,不管是运用PRP或PRF还是传统BC作为支架,其在临床治愈率、牙根长度或者根管壁厚度增加率和根尖封闭率上无明显差异,但是适当地使用PRP或PRF支架材料可在一定程度上提高牙根长度增加量。展开更多
文摘Morphological and radiological methods were used to study regeneration of the damaged bone of rat mandibles after application of plateletenriched fibrin clot. A bone hole was artificially created, and in the natural course of regeneration, the hole was immediately filled with blood and there a blood clot formed. After one week of healing, separate islands of young bone tissue appeared. After two to three weeks, the opening in the mandible was completely replaced by the young bone tissue. When a similar bone hole was filled with autological fibrin clot, the blood clot did not form. But after one week the entire hole was filed with newlyformed fused bone tissue. By the second week after the use of fibrin clot, the bone hole had further healed and bone callus was formed.
基金the National Research Foundation of Korea,funded by the Korean Ministry of Education,Sci-ence and Technology,No.2007-0055330Inje Univer-sity Research Grant (2008)
文摘Cheonggukjang is a soybean paste made by fermenting whole cooked soybeans with Bacillus subtilis. Cheonggukjang contains a fibrinolytic enzyme that could provide clinical applications for removing blood clots. In the present study, the term ＂cheonggukjang kinase＂ （CGK） was used to refer to this fibrinolytic enzyme. The thrombolytic effects of CGK were analyzed in a rat model of cerebral embolic stroke produced by middle cerebral artery occlusion （MCAO）. Results from fibrin and platelet-rich clot lysis assays demonstrated that thrombolytic activity was greatest in CGKs, which were cultured for 40 hours. In addition, T50, the time needed to decompose 50% of the clot, did not change with plasminogen treatment, indicating that CGK was not a plasminogen activator, but was rather presumed to act as a plasmin-like protein. An intravenous infusion of CGK （1 U plasmin-like activity/100 μg CGK/kg） at 1 hour after MCAO resulted in removal of clots in a rat model of cerebral embolic stroke. CGK-treated groups exhibited a significant dose-dependent reduction in infarct volume. CGK treatment also improved functional recovery, as assessed by neurological deficit scores. Decreased infarct volume and improved functional recovery following CGK treatment was greater compared with recombinant tissue plasminogen activator （10 mg/kg）. These results suggested that CGK effectively reduced infarct volume and improved functional recovery following ischemic brain injury. CGK exhibits a number of potential clinical applications ir the treatment of cerebral embolic stroke.
文摘Background: Many surgical haemostatic agents are available which aim to reduce morbidity and mortality of bleeding, as well as associated costs. We used qualitative techniques to compare the effectiveness of two topical gelatine-based haemostatic agents in a porcine liver trauma model. Methods: We compared the activity of Floseal? (with human or bovine thrombin), Surgiflo? and Surgiflo? plus 5000 IU bovine thrombin per 10 mL of product (n = 6 test sites/group). Different clinical scenarios were evaluated in two anaesthetised piglets;normal physiological conditions (normotension and normothermia), and a status of hypotension, hypothermia and haemodilution, requiring inotropic support. Laparotomy was performed, and five identical stab wounds were made on each liver lobe. Four of these wounds were randomly treated with one of the agents under investigation, while one wound was kept as an untreated control. Haemostasis was observed during and immediately following surgery. After euthanisation, wounds were examined (blinded for treatment) macroscopically, microscopically and using scanning electron microscopic evaluation. Results: Surgiflo? produced some degree of clotting in 1/3 applications in both piglets. Co-treatment with thrombin resulted in a minor improvement in performance in the animal with normal physiological conditions (some degree of clotting in all wounds);no improvement was seen in the animal in a status of hypotension, hypothermia and haemodilution. Floseal? induced clotting in all wounds for both piglets. Microscopic and scanning electron microscope examination of the stab wounds demonstrated that Floseal? created a stable, dense agglomerate of gelatine and fibrin, which firmly adhered to the adjacent liver tissue. In wounds treated with Surgiflo? or Surgiflo? plus thrombin, the gelatine contained more air bubbles, resulting in less fibrin inclusion in the clot and weaker adhesion of the clot to the liver tissue. Conclusion: Floseal? created a dense and stable blood clot, even in a piglet with hypotension, hypothermia and haemodilution.
文摘目的评价牙髓血运重建时运用富血小板血浆(PRP)或富血小板纤维蛋白(PRF)同传统的血凝块(BC)作为支架哪种材料效果更佳。方法检索PubMed、Embase、Cochrane、Web of Science、中国期刊全文数据库(CNKI)和万方数字期刊全文数据库,检索时间截止到2020年8月。通过RevMan 5.3和stata 13.0软件对纳入的研究的临床治愈率、根长、根管壁厚度和根尖封闭的疗效进行Meta分析。结果当牙髓血运重建时分别运用PRP或PRF同BC在临床治愈率和根尖封闭率上均无差异,与BC相比,PRP和PRF的临床治愈率的相对危险度(RR)为0.99(0.87,1.12),95%的置信区间(CI)为0.95(0.86,1.04),根尖封闭率的RR为1.09(0.91,1.30)和95%CI为1.02(0.83,1.25)。对牙根长度或根管壁厚度增加率分析时发现PRP或PRF同BC之间无差异,与BC相比,PRP和PRF的牙根长度增加率的RR及95%CI分别为1.06(0.86,1.32)和1.10(0.90,1.35),根管壁厚度增加率的RR及95%CI分别为1.04(0.86,1.26)和1.09(0.76,1.57)。对牙根长度增加量分析时发现PRP优于BC,其加权均数差(WMD)及95%CI为1.47(0.57,2.36)mm。结论在行牙髓血运重建时,不管是运用PRP或PRF还是传统BC作为支架,其在临床治愈率、牙根长度或者根管壁厚度增加率和根尖封闭率上无明显差异,但是适当地使用PRP或PRF支架材料可在一定程度上提高牙根长度增加量。
