The organization of the compartment of mesenchymal stem cells is still obscure. Two types of human stromal precursor cells are known. Both of them are analyzed in in vitro system: mesenchymal multipotent stromal cells...The organization of the compartment of mesenchymal stem cells is still obscure. Two types of human stromal precursor cells are known. Both of them are analyzed in in vitro system: mesenchymal multipotent stromal cells (MMSC) and fibroblast colony forming units (CFU-F). The aim of this study was to compare the main characteristics of MMSC and CFU-F derived from the bone marrow of 24 healthy donors. Growth and differentiation parameters, as well as relative expression levels of different genes were analyzed in MMSC and CFU-F. MMSC were cultivated for 5 passages. CFU-F concentration was determined for each bone marrow sample. The data obtained demonstrated the heterogeneity and hierarchical organization of both studied populations of stromal precursor cells-MMSC and CFU-F. These two types of stromal precursor cells turned to be different in most parameters studied. Altogether MMSC seemed to be more immature cells than CFU-F and took up the higher position in hierarchical tree of mesenchymal stem cells. The rate of differentiation and proliferative potential decreased with the donor’s age in both populations MMSC and CFU-F.展开更多
Non-adherent bone marrow cell-derived mesenchymal stem cells from C57BL/6J mice were sepa- rated and cultured using the ＂pour-off＂ method. Non-adherent bone marrow cell-derived mesen- chymal stem ceils developed col...Non-adherent bone marrow cell-derived mesenchymal stem cells from C57BL/6J mice were sepa- rated and cultured using the ＂pour-off＂ method. Non-adherent bone marrow cell-derived mesen- chymal stem ceils developed colony-forming unit-fibroblasts, and could be expanded by supple- mentation with epidermal growth factor. Immunocytochemistry showed that the non-adherent bone marrow cell-derived mesenchymal stem cells exposed to basic fibroblast growth factor/epidermal growth factor/nerve growth factor expressed the neuron specific markers, neurofilament-200 and NeuN, in vitro. Non-adherent bone marrow cell-derived mesenchymal stem cells from 13-galactosidase transgenic mice were also transplanted into focal ischemic brain （right corpus striatum） of C57BL/6J mice. At 8 weeks, cells positive for LacZ and 13-galactosidase staining were observed in the ischemic tissues, and cells co-labeled with both 13-galactosidase and NeuN were seen by double immunohistochemical staining. These findings suggest that the non-adherent bone marrow cell-derived mesenchymal stem cells could differentiate into neuronal-like cells in vitro and in vivo.展开更多
Objective To investigate the effect of lithium on the growth of bone marrow stromal cells Methods We compared the different effects of lithium with the concentration of 0 1-12mmol/L on the growth of murine fibrobl...Objective To investigate the effect of lithium on the growth of bone marrow stromal cells Methods We compared the different effects of lithium with the concentration of 0 1-12mmol/L on the growth of murine fibroblast colony forming unit (mCFU F), human fibroblast colony forming unit (hCFU F) and their intercolonial cells Pure murine bone marrow stromal cell assay was used to investigate the mechanism Results Cultures with 1-4mmol/L lithium resulted in significant increase in the number of hCFU F, but distinct decrease in the number of mCFU F Furthermore, there were much more endothelial cells between mCFU Fs than those between hCFU Fs The direct effect of 1 4mmol/L lithium on the growth of pure murine fibroblasts and endothelial cells was stimulatory Serum free endothelial cell conditioned medium (EC CM) could inhibit the growth of mCFU F Conclusions 1-4mmol/L lithium has stimulatory effect on the growth of pure murine fibroblasts and endothelial cells The inhibitory effect of lithium on mCFU F formation may result from the effect of increased endothelial cells展开更多
文摘The organization of the compartment of mesenchymal stem cells is still obscure. Two types of human stromal precursor cells are known. Both of them are analyzed in in vitro system: mesenchymal multipotent stromal cells (MMSC) and fibroblast colony forming units (CFU-F). The aim of this study was to compare the main characteristics of MMSC and CFU-F derived from the bone marrow of 24 healthy donors. Growth and differentiation parameters, as well as relative expression levels of different genes were analyzed in MMSC and CFU-F. MMSC were cultivated for 5 passages. CFU-F concentration was determined for each bone marrow sample. The data obtained demonstrated the heterogeneity and hierarchical organization of both studied populations of stromal precursor cells-MMSC and CFU-F. These two types of stromal precursor cells turned to be different in most parameters studied. Altogether MMSC seemed to be more immature cells than CFU-F and took up the higher position in hierarchical tree of mesenchymal stem cells. The rate of differentiation and proliferative potential decreased with the donor’s age in both populations MMSC and CFU-F.
基金supported by the National Natural Science Foundation of China,No.30471836
文摘Non-adherent bone marrow cell-derived mesenchymal stem cells from C57BL/6J mice were sepa- rated and cultured using the ＂pour-off＂ method. Non-adherent bone marrow cell-derived mesen- chymal stem ceils developed colony-forming unit-fibroblasts, and could be expanded by supple- mentation with epidermal growth factor. Immunocytochemistry showed that the non-adherent bone marrow cell-derived mesenchymal stem cells exposed to basic fibroblast growth factor/epidermal growth factor/nerve growth factor expressed the neuron specific markers, neurofilament-200 and NeuN, in vitro. Non-adherent bone marrow cell-derived mesenchymal stem cells from 13-galactosidase transgenic mice were also transplanted into focal ischemic brain （right corpus striatum） of C57BL/6J mice. At 8 weeks, cells positive for LacZ and 13-galactosidase staining were observed in the ischemic tissues, and cells co-labeled with both 13-galactosidase and NeuN were seen by double immunohistochemical staining. These findings suggest that the non-adherent bone marrow cell-derived mesenchymal stem cells could differentiate into neuronal-like cells in vitro and in vivo.
文摘Objective To investigate the effect of lithium on the growth of bone marrow stromal cells Methods We compared the different effects of lithium with the concentration of 0 1-12mmol/L on the growth of murine fibroblast colony forming unit (mCFU F), human fibroblast colony forming unit (hCFU F) and their intercolonial cells Pure murine bone marrow stromal cell assay was used to investigate the mechanism Results Cultures with 1-4mmol/L lithium resulted in significant increase in the number of hCFU F, but distinct decrease in the number of mCFU F Furthermore, there were much more endothelial cells between mCFU Fs than those between hCFU Fs The direct effect of 1 4mmol/L lithium on the growth of pure murine fibroblasts and endothelial cells was stimulatory Serum free endothelial cell conditioned medium (EC CM) could inhibit the growth of mCFU F Conclusions 1-4mmol/L lithium has stimulatory effect on the growth of pure murine fibroblasts and endothelial cells The inhibitory effect of lithium on mCFU F formation may result from the effect of increased endothelial cells
