Epidemiology and molecular genotyping of echinostome metacercariae in Filopaludina snails in Lamphun Province,Thailand

Objective:To analyze the prevalence of echinostome metacercariae in Filopaludina dorliaris(F.dorliaris)and Filopaludina martensi martensi(F.martensi martensi)and genotype variation of echinostome metacercariae by using random amplified polymorphic DNA(RAPD)analysis.Methods:Filopaludina sp.snails were collected from eight localities of Lamphun Province,Northern Thailand and examined for echinostome metacercariae.RAPD-PCR was used lo analyze genotype variation of echinostome metacercariae.Results:A total of 3 226 F.dorliaris and F.martensi martensi snails were collected from eight localities.The overall prevalences of echinostome metacercariae in F.dorliaris and F.martensi martensi were 40.89%and 36.27%,while the intensity of infection was 20.37 and 12.04,respectively.The dendrogram constructed base on RAPD profiles,4 well supported domains were generated;(i)group of metacercariae from Ban Hong,Mae Ta,Meaung,Pa Sang,Toong Hua Chang,and Weang Nong that were clustered in the group of E.revolutum,(ii)Ban Thi,(iii)Lee,and(iv)3 adults of an out group.Condusioiis:This research demonstrated RAPD profiling has been a useful tool to detect DNA polymorphisms to determine genetic relationship between echinostome metacercariae in Lamphun Province,Northern Thailand.

蜗牛多糖体外抗乙型肝炎病毒作用研究

目的:探讨蜗牛多糖抗乙肝病毒活性。方法:将不同浓度蜗牛多糖与HepG2.2.15细胞株共培养,以拉米夫定为阳性对照药,用ELISA法检测HBsAg和HBeAg分泌水平,实时荧光定量PCR检测HBV DNA含量,计算抑制率。结果:蜗牛多糖在体外能有效抑制HepG2.2.15细胞HBsAg和HBeAg分泌,最大抑制率分别为42.8%和52.1%;对HBV DNA的复制有一定抑制作用(P<0.05)。结论:蜗牛多糖在体外具有显著的抗乙型肝炎病毒作用,且毒性较小,具有良好应用前景。

短咽类吸虫Brachylaima sp.幼虫期形态及其感染情况调查

报道了短咽类吸虫Ｂｒａｃｈｙｌａｉｍａｓｐ．在中间宿主陆地蜗牛中的幼虫期：胞蚴、尾蚴和后蚴的形态及其在螺体中的自然感染情况的调查．发现尾蚴的尾部后排泄管的形态及开口位置不同于已报告种类；其在同型巴蜗牛Ｂｒａｄｙｂａｅｎａｓｉｍｉｌａｒｉｓ中的感染一年有一个高峰，主要在秋季，感染率达到３．０２％，春季感染率最低；一年的平均感染率为０．４２６６％．

厦门岛陆地蜗牛自然感染吸虫幼虫期种类的调查研究

陆地蜗牛是可供多种重要寄生虫作为中间宿主的种类，其中对人及牛羊等经济动物危害严重的双腔科吸虫即是需要陆地蜗牛作为它的第１中间宿主（唐仲璋和唐崇惕１９７５［１］，１９７７［２］１９７８［３］），也是寄生在鸟类和哺乳类的短咽科吸虫、寄生在两栖类的半肠科吸...

镇赉县片形吸虫和东毕吸虫幼虫在螺体内发育的季节动态调查研究

对吉林省镇赉县两牧场的小土蜗(Galba pervia)和耳萝卜螺(Radix auricularia)体内片形吸虫(Fasciolas sp)、东毕吸虫(Orientobicharzia sp)幼虫做了调查。结果为,东毕吸虫尾蚴6月下旬开始从螺体内逸出,8月中旬达最高峰,9月下旬逐渐减少。片形吸虫尾蚴7月中旬开始从螺体内逸出,9月上旬达最高峰,10月上旬阳性螺逐渐减少。含两种吸虫幼虫的阳性螺率与气温和降雨量呈正相关关系;片形吸虫幼虫可以以雷蚴的形式在螺体内越冬,该地区含东毕吸虫幼虫的阳性螺率比含片形吸虫幼虫的阳性螺率高。含东毕吸虫尾蚴的阳性螺率最高达52.52%(252/480), 而含片形吸虫尾蚴的阳性螺率最高只达17.33%(52/300)。