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Poly(N-isopropylacrylamide)-grafted Dual Stimuli-responsive Filter Paper for Protein Separation 被引量:1
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作者 Qi-jia-yu Wu Rui Wang +3 位作者 Ying Zhou Ya-qin Huang Raja Ghosh 陈晓农 《Chinese Journal of Polymer Science》 SCIE CAS CSCD 2015年第7期1048-1057,共10页
Thermal and salt dual stimuli-responsive filter-paper-based membranes were prepared by UV-induced grafting of NIPAM-based polymers on paper surface. The grafting ratio could be controlled by monomer concentration duri... Thermal and salt dual stimuli-responsive filter-paper-based membranes were prepared by UV-induced grafting of NIPAM-based polymers on paper surface. The grafting ratio could be controlled by monomer concentration during grafting polymerization. The results from pressure drop measurement of the mobile phase flowed cross the membrane demonstrate that an appropriate grafting ratio would be 8%-10%. Protein adsorption on the membrane through hydrophobic interaction could be promoted by increasing temperature and lyotropic salt concentration. The effect of grafted polymer structure on protein binding performance was studied. Filter paper grafted with NIPAM-based branched copolymer consisting of hydrophobic monomer moieties shows ten times higher protein binding capacity than that of the original filter paper. The separation of plasma proteins using the dual stimuli-responsive membrane was examined to demonstrate feasible application for hydrophobic interaction chromatographic separation of proteins. 展开更多
关键词 N-isopropyl acrylamide filter paper Surface grafting Stimuli-responsive hydrophobic transition Protein separation
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