[Objective] This paper aimed to study the mechanism of diarrhea of mink caused by Escherichia coil [Method] Through the detection of K88 fimbriae gene of E. coli, cloning of gene fragments and identification, then PCR...[Objective] This paper aimed to study the mechanism of diarrhea of mink caused by Escherichia coil [Method] Through the detection of K88 fimbriae gene of E. coli, cloning of gene fragments and identification, then PCR amplification was used to detect adhesion factor K88 gene, which was connected to T-vector and transformed into competent cells, and positive clones were selected. [ Results] E. coli 078, 029 and 038 were isolated from organs and feces of mink died of diarrhea in 3 mink farms, respectively, the 3 serotypes of E. coliwere detected in carrying K88 fimbriae gene and 3 positive clones were screened, respectively. [ Conclusion] The E. coli causing mink diarrhea carry K88 fimbriae gene.展开更多
基金China Postdoctoral Sustentation Fund(NO.20100470565)Hebei Sustain Program of Science and Technology(NO.10960408D)Qinhuangdao Scientific and Technological Development Program(NO.201101A182)
文摘[Objective] This paper aimed to study the mechanism of diarrhea of mink caused by Escherichia coil [Method] Through the detection of K88 fimbriae gene of E. coli, cloning of gene fragments and identification, then PCR amplification was used to detect adhesion factor K88 gene, which was connected to T-vector and transformed into competent cells, and positive clones were selected. [ Results] E. coli 078, 029 and 038 were isolated from organs and feces of mink died of diarrhea in 3 mink farms, respectively, the 3 serotypes of E. coliwere detected in carrying K88 fimbriae gene and 3 positive clones were screened, respectively. [ Conclusion] The E. coli causing mink diarrhea carry K88 fimbriae gene.
