Tripartite motif(TRIM)proteins were shown to play an important role in innate antiviral immunity.FinTRIM(ftr)is a new subset of TRIM genes that do not possess obvious orthologs in higher vertebrates.However,little is ...Tripartite motif(TRIM)proteins were shown to play an important role in innate antiviral immunity.FinTRIM(ftr)is a new subset of TRIM genes that do not possess obvious orthologs in higher vertebrates.However,little is known about its function.In this study,we used bioinformatic analysis to examine the phylogenetic relationships and conserved domains of zebrafish(Danio rerio)fir01,fir42,and fir58.as well as qualitative real-time PCR to examine their expression patterns in zebrafish embryonic fibroblast(ZF4)cells and zebrafish tissues.Sequence analysis showed that the three finTRIMs are highly conserved,and all contain a RING domain.B-box domain,and SPRY-PRY domain.In addition,fr42 and fir58 had one coiled-coil domain(CCD),whereas ftrol had two CCDs.Tissue expression analysis revealed that the mRNA level of ftr01 was the highest in the liver,whereas those of ftr42 and ftr58 were the highest in the gill;the expression of thesefinTRIMs was clearly upregulated not in the eyes,but in the liver,spleen,kidney,gill,and brain of zebrafish following spring viremia of carp virus(SVCV)infection.Similarly,the expression of these three finTRIM genes also increased in ZF4 cells after SVCV infection.Our study revealed that fir01,fr42,and fir58 may play an important role in antiviralimmune responses,and these findings validate the need for more in-depth research on the finTRIM family in the future.展开更多
基金supported by the National Training Program of Innovation and Entrepreneurship for Undergraduates of Huazhong Agricultural University (2015308200403)the National Key Research and Development Program of China (2018YFD0900505)+1 种基金the Natural Science Foundation of China (31172433)the Fundamental Research Funds for the Central Universities (2662018YJ022)
文摘Tripartite motif(TRIM)proteins were shown to play an important role in innate antiviral immunity.FinTRIM(ftr)is a new subset of TRIM genes that do not possess obvious orthologs in higher vertebrates.However,little is known about its function.In this study,we used bioinformatic analysis to examine the phylogenetic relationships and conserved domains of zebrafish(Danio rerio)fir01,fir42,and fir58.as well as qualitative real-time PCR to examine their expression patterns in zebrafish embryonic fibroblast(ZF4)cells and zebrafish tissues.Sequence analysis showed that the three finTRIMs are highly conserved,and all contain a RING domain.B-box domain,and SPRY-PRY domain.In addition,fr42 and fir58 had one coiled-coil domain(CCD),whereas ftrol had two CCDs.Tissue expression analysis revealed that the mRNA level of ftr01 was the highest in the liver,whereas those of ftr42 and ftr58 were the highest in the gill;the expression of thesefinTRIMs was clearly upregulated not in the eyes,but in the liver,spleen,kidney,gill,and brain of zebrafish following spring viremia of carp virus(SVCV)infection.Similarly,the expression of these three finTRIM genes also increased in ZF4 cells after SVCV infection.Our study revealed that fir01,fr42,and fir58 may play an important role in antiviralimmune responses,and these findings validate the need for more in-depth research on the finTRIM family in the future.
