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Optimizing Enterprise Conversational AI: Accelerating Response Accuracy with Custom Dataset Fine-Tuning
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作者 Yash Kishore 《Intelligent Information Management》 2024年第2期65-76,共12页
As the realm of enterprise-level conversational AI continues to evolve, it becomes evident that while generalized Large Language Models (LLMs) like GPT-3.5 bring remarkable capabilities, they also bring forth formidab... As the realm of enterprise-level conversational AI continues to evolve, it becomes evident that while generalized Large Language Models (LLMs) like GPT-3.5 bring remarkable capabilities, they also bring forth formidable challenges. These models, honed on vast and diverse datasets, have undoubtedly pushed the boundaries of natural language understanding and generation. However, they often stumble when faced with the intricate demands of nuanced enterprise applications. This research advocates for a strategic paradigm shift, urging enterprises to embrace a fine-tuning approach as a means to optimize conversational AI. While generalized LLMs are linguistic marvels, their inability to cater to the specific needs of businesses across various industries poses a critical challenge. This strategic shift involves empowering enterprises to seamlessly integrate their own datasets into LLMs, a process that extends beyond linguistic enhancement. The core concept of this approach centers on customization, enabling businesses to fine-tune the AI’s functionality to fit precisely within their unique business landscapes. By immersing the LLM in industry-specific documents, customer interaction records, internal reports, and regulatory guidelines, the AI transcends its generic capabilities to become a sophisticated conversational partner aligned with the intricacies of the enterprise’s domain. The transformative potential of this fine-tuning approach cannot be overstated. It enables a transition from a universal AI solution to a highly customizable tool. The AI evolves from being a linguistic powerhouse to a contextually aware, industry-savvy assistant. As a result, it not only responds with linguistic accuracy but also with depth, relevance, and resonance, significantly elevating user experiences and operational efficiency. In the subsequent sections, this paper delves into the intricacies of fine-tuning, exploring the multifaceted challenges and abundant opportunities it presents. It addresses the technical intricacies of data integration, ethical considerations surrounding data usage, and the broader implications for the future of enterprise AI. The journey embarked upon in this research holds the potential to redefine the role of conversational AI in enterprises, ushering in an era where AI becomes a dynamic, deeply relevant, and highly effective tool, empowering businesses to excel in an ever-evolving digital landscape. 展开更多
关键词 fine-tuning DATASET AI CONVERSATIONAL ENTERPRISE LLM
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Rotary-scaling fine-tuning (RSFT) method for optimizing railway wheel profiles and its application to a locomotive 被引量:7
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作者 Yunguang Ye Yayun Qi +3 位作者 Dachuan Shi Yu Sun Yichang Zhou Markus Hecht 《Railway Engineering Science》 2020年第2期160-183,共24页
The existing multi-objective wheel profile optimization methods mainly consist of three sub-modules:(1)wheel profile generation,(2)multi-body dynamics simulation,and(3)an optimization algorithm.For the first module,a ... The existing multi-objective wheel profile optimization methods mainly consist of three sub-modules:(1)wheel profile generation,(2)multi-body dynamics simulation,and(3)an optimization algorithm.For the first module,a comparably conservative rotary-scaling finetuning(RSFT)method,which introduces two design variables and an empirical formula,is proposed to fine-tune the traditional wheel profiles for improving their engineering applicability.For the second module,for the TRAXX locomotives serving on the Blankenburg–Rubeland line,an optimization function representing the relationship between the wheel profile and the wheel–rail wear number is established based on Kriging surrogate model(KSM).For the third module,a method combining the regression capability of KSM with the iterative computing power of particle swarm optimization(PSO)is proposed to quickly and reliably implement the task of optimizing wheel profiles.Finally,with the RSFT–KSM–PSO method,we propose two wear-resistant wheel profiles for the TRAXX locomotives serving on the Blankenburg–Rubeland line,namely S1002-S and S1002-M.The S1002-S profile minimizes the total wear number by 30%,while the S1002-M profile makes the wear distribution more uniform through a proper sacrifice of the tread wear number,and the total wear number is reduced by 21%.The quasi-static and hunting stability tests further demonstrate that the profile designed by the RSFT–KSM–PSO method is promising for practical engineering applications. 展开更多
关键词 Wheel profile optimization Wear reduction Rotary-scaling fine-tuning Particle swarm optimization Kriging surrogate model
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Railway wheel profile fine-tuning system for profile recommendation 被引量:3
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作者 Yunguang Ye Jonas Vuitton +1 位作者 Yu Sun Markus Hecht 《Railway Engineering Science》 2021年第1期74-93,共20页
This paper develops a wheel profile fine-tuning system(WPFTS)that comprehensively considers the influence of wheel profile on wheel damage,vehicle stability,vehicle safety,and passenger comfort.WPFTS can recommend one... This paper develops a wheel profile fine-tuning system(WPFTS)that comprehensively considers the influence of wheel profile on wheel damage,vehicle stability,vehicle safety,and passenger comfort.WPFTS can recommend one or more optimized wheel profiles according to train operators’needs,e.g.,reducing wheel wear,mitigating the development of wheel out-of-roundness(OOR),improving the shape stability of the wheel profile.Specifically,WPFTS includes four modules:(I)a wheel profile generation module based on the rotary-scaling finetuning(RSFT)method;(II)a multi-objective generation module consisting of a rigid multi-body dynamics simulation(MBS)model,an analytical model,and a rigid–flexible MBS model,for generating 11 objectives related to wheel damage,vehicle stability,vehicle safety,and passenger comfort;(III)a weight assignment module consisting of an adaptive weight assignment strategy and a manual weight assignment strategy;and(IV)an optimization module based on radial basis function(RBF)and particle swarm optimization(PSO).Finally,three cases are introduced to show how WPTFS recommends a wheel profile according to train operators’needs.Among them,a wheel profile with high shape stability,a wheel profile for mitigating the development of wheel OOR,and a wheel profile considering hunting stability and derailment safety are developed,respectively. 展开更多
关键词 Wheel profile fine-tuning system Optimization RECOMMENDATION WEAR Contact concentration index Multi-body dynamics simulation(MBS) Railway wheel
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Fine-tuning of cortical progenitor proliferation by thalamic afferents
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作者 Katrin Gerstmann Geraldine Zimmer 《Neural Regeneration Research》 SCIE CAS CSCD 2015年第6期887-888,共2页
During cerebral cortical cortex neurogenesis two major types of progenitors generate a variety of morphologically and functionally diverse projection neurons destined for the different cortical layers in non-gyrified ... During cerebral cortical cortex neurogenesis two major types of progenitors generate a variety of morphologically and functionally diverse projection neurons destined for the different cortical layers in non-gyrified mice. Radial glia cells (RGCs) undergo mitosis in the cortical ventricular zone and exhibit an apical-basal cell polarity, whereas non-polar intermediate progenitor cells (IPCs) divide basally in the subventricular zone (Franco and Muller, 2013; Taverna et al., 2014). 展开更多
关键词 Eph fine-tuning of cortical progenitor proliferation by thalamic afferents
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New approach to assess sperm DNA fragmentation dynamics: Fine-tuning mathematical models
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作者 Isabel Ortiz Jesus Dorado +4 位作者 Jane Morrell Jaime Gosalvez Francisco Crespo Juan M.Jimenez Manuel Hidalgo 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2017年第3期592-600,共9页
Background: Sperm DNA fragmentation(sDF) has been proved to be an important parameter in order to predict in vitro the potential fertility of a semen sample. Colloid centrifugation could be a suitable technique to ... Background: Sperm DNA fragmentation(sDF) has been proved to be an important parameter in order to predict in vitro the potential fertility of a semen sample. Colloid centrifugation could be a suitable technique to select those donkey sperm more resistant to DNA fragmentation after thawing. Previous studies have shown that to elucidate the latent damage of the DNA molecule, sDF should be assessed dynamically, where the rate of fragmentation between treatments indicates how resistant the DNA is to iatrogenic damage. The rate of fragmentation is calculated using the slope of a linear regression equation. However, it has not been studied if s DF dynamics fit this model. The objectives of this study were to evaluate the effect of different after-thawing centrifugation protocols on sperm DNA fragmentation and elucidate the most accurate mathematical model(linear regression, exponential or polynomial) for DNA fragmentation over time in frozen-thawed donkey semen.Results: After submitting post-thaw semen samples to no centrifugation(UDC), sperm washing(SW) or single layer centrifugation(SLC) protocols, sD F values after 6 h of incubation were significantly lower in SLC samples than in SW or UDC.Coefficient of determination(R-2) values were significantly higher for a second order polynomial model than for linear or exponential. The highest values for acceleration of fragmentation(aSDF) were obtained for SW, fol owed by SLC and UDC.Conclusion: SLC after thawing seems to preserve longer DNA longevity in comparison to UDC and SW. Moreover,the fine-tuning of models has shown that sDF dynamics in frozen-thawed donkey semen fit a second order polynomial model, which implies that fragmentation rate is not constant and fragmentation acceleration must be taken into account to elucidate hidden damage in the DNA molecule. 展开更多
关键词 Colloid centrifugation Dynamics fine-tuning Mathematical models Sperm DNA fragmentation
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Fine-Tuning Bilateral Ties
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作者 Ni Yanshuo 《ChinAfrica》 2011年第2期14-17,共4页
Chinese Vice Premier’s visit to Africa continues to emphasize the mutual cooperation,with a focus on agriculture FOR many years,the Chinese Government has dispatched the minister of foreign affairs to Africa for the ... Chinese Vice Premier’s visit to Africa continues to emphasize the mutual cooperation,with a focus on agriculture FOR many years,the Chinese Government has dispatched the minister of foreign affairs to Africa for the first official visit of a year.This year,however,that rule was broken when Hui Liangyu,Chinese Vice Premier,made the 14-day trip. On January 6-19,Hui paid official visits to Mauritius,Zambia,the Democratic Republic of Congo(DRC),Cameroon and Senegal,focusing on economic and agri- 展开更多
关键词 fine-tuning Bilateral Ties DRC
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Improving BERT Fine-Tuning via Self-Ensemble and Self-Distillation
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作者 许一格 邱锡鹏 +1 位作者 周浬皋 黄萱菁 《Journal of Computer Science & Technology》 SCIE EI CSCD 2023年第4期853-866,共14页
Fine-tuning pre-trained language models like BERT have become an effective way in natural language processing(NLP)and yield state-of-the-art results on many downstream tasks.Recent studies on adapting BERT to new task... Fine-tuning pre-trained language models like BERT have become an effective way in natural language processing(NLP)and yield state-of-the-art results on many downstream tasks.Recent studies on adapting BERT to new tasks mainly focus on modifying the model structure,re-designing the pre-training tasks,and leveraging external data and knowledge.The fine-tuning strategy itself has yet to be fully explored.In this paper,we improve the fine-tuning of BERT with two effective mechanisms:self-ensemble and self-distillation.The self-ensemble mechanism utilizes the checkpoints from an experience pool to integrate the teacher model.In order to transfer knowledge from the teacher model to the student model efficiently,we further use knowledge distillation,which is called self-distillation because the distillation comes from the model itself through the time dimension.Experiments on the GLUE benchmark and the Text Classification benchmark show that our proposed approach can significantly improve the adaption of BERT without any external data or knowledge.We conduct exhaustive experiments to investigate the efficiency of the self-ensemble and self-distillation mechanisms,and our proposed approach achieves a new state-of-the-art result on the SNLI dataset. 展开更多
关键词 BERT deep learning fine-tuning natural language processing(NLP) pre-training model
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Classification of Conversational Sentences Using an Ensemble Pre-Trained Language Model with the Fine-Tuned Parameter
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作者 R.Sujatha K.Nimala 《Computers, Materials & Continua》 SCIE EI 2024年第2期1669-1686,共18页
Sentence classification is the process of categorizing a sentence based on the context of the sentence.Sentence categorization requires more semantic highlights than other tasks,such as dependence parsing,which requir... Sentence classification is the process of categorizing a sentence based on the context of the sentence.Sentence categorization requires more semantic highlights than other tasks,such as dependence parsing,which requires more syntactic elements.Most existing strategies focus on the general semantics of a conversation without involving the context of the sentence,recognizing the progress and comparing impacts.An ensemble pre-trained language model was taken up here to classify the conversation sentences from the conversation corpus.The conversational sentences are classified into four categories:information,question,directive,and commission.These classification label sequences are for analyzing the conversation progress and predicting the pecking order of the conversation.Ensemble of Bidirectional Encoder for Representation of Transformer(BERT),Robustly Optimized BERT pretraining Approach(RoBERTa),Generative Pre-Trained Transformer(GPT),DistilBERT and Generalized Autoregressive Pretraining for Language Understanding(XLNet)models are trained on conversation corpus with hyperparameters.Hyperparameter tuning approach is carried out for better performance on sentence classification.This Ensemble of Pre-trained Language Models with a Hyperparameter Tuning(EPLM-HT)system is trained on an annotated conversation dataset.The proposed approach outperformed compared to the base BERT,GPT,DistilBERT and XLNet transformer models.The proposed ensemble model with the fine-tuned parameters achieved an F1_score of 0.88. 展开更多
关键词 Bidirectional encoder for representation of transformer conversation ensemble model fine-tuning generalized autoregressive pretraining for language understanding generative pre-trained transformer hyperparameter tuning natural language processing robustly optimized BERT pretraining approach sentence classification transformer models
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Mining and fine-tuning sugar uptake system for titer improvement of milbemycins in Streptomyces bingchenggensis 被引量:1
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作者 Pinjiao Jin Shanshan Li +4 位作者 Yanyan Zhang Liyang Chu Hairong He Zhuoxu Dong Wensheng Xiang 《Synthetic and Systems Biotechnology》 SCIE 2020年第3期214-221,共8页
Dramatic decrease of sugar uptake is a general phenomenon in Streptomyces at stationary phase,when antibiotics are extensively produced.Milbemycins produced by Streptomyces bingchenggensis are a group of valuable macr... Dramatic decrease of sugar uptake is a general phenomenon in Streptomyces at stationary phase,when antibiotics are extensively produced.Milbemycins produced by Streptomyces bingchenggensis are a group of valuable macrolide biopesticides,while the low yield and titer impede their broad applications in agricultural field.Considering that inadequate sugar uptake generally hinders titer improvement of desired products,we mined the underlying sugar uptake systems and fine-tuned their expression in this work.First,we screened the candidates at both genomic and transcriptomic level in S.bingchenggensis.Then,two ATP-binding cassette transporters named TP2 and TP5 were characterized to improve milbemycin titer and yield significantly.Next,the appropriate native temporal promoters were selected and used to tune the expression of TP2 and TP5,resulting in a maximal milbemycin A3/A4 titer increase by 36.9%to 3321 mg/L.Finally,TP2 and TP5 were broadly finetuned in another two macrolide biopesticide producers Streptomyces avermitilis and Streptomyces cyaneogriseus,leading to a maximal titer improvement of 34.1%and 52.6%for avermectin B1a and nemadectin,respectively.This work provides useful transporter tools and corresponding engineering strategy for Streptomyces. 展开更多
关键词 STREPTOMYCES Sugar uptake system fine-tuning Titer improvement Milbemycins Macrolide biopesticides
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Fine-Tuning Government Investment
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作者 Wang Jun 《Beijing Review》 2019年第21期34-35,共2页
A long--expected regulation to guide public fund investment is issued Chinese Premier Li Keqiang signed a State Council decree on May 5 to promulgate a regulation on government investment, which will take effect on Ju... A long--expected regulation to guide public fund investment is issued Chinese Premier Li Keqiang signed a State Council decree on May 5 to promulgate a regulation on government investment, which will take effect on July 1. 展开更多
关键词 In fine-tuning GOVERNMENT INVESTMENT
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民间文学文本命名实体识别方法
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作者 黄健钰 王笳辉 +1 位作者 段亮 冉苒 《软件导刊》 2023年第10期65-72,共8页
民间文学文本命名实体识别任务旨在从民间文学文本中判别实体并将其划分到预定义的语义类别,为民间文学的保存与传播奠定基础。民间文学区别于一般中文语料,其文本存在一词多义情况突出与领域名词众多的问题,导致常规命名实体识别方法... 民间文学文本命名实体识别任务旨在从民间文学文本中判别实体并将其划分到预定义的语义类别,为民间文学的保存与传播奠定基础。民间文学区别于一般中文语料,其文本存在一词多义情况突出与领域名词众多的问题,导致常规命名实体识别方法难以准确充分地识别出民间文学文本中存在的实体及其类别。针对该问题,提出一种基于BERT的民间文学文本命名实体识别模型TBERT。该模型首先在通用中文BERT模型的基础上融合民间文学文本语料特征与实体类型特征;然后利用BiLSTM模型进一步提取序列依赖特征;最后结合CRF模型获取的标签约束信息输出全局最优结果。实验结果表明,该方法在民间文学文本数据集上具有良好表现。 展开更多
关键词 民间文学文本 命名实体识别 fine-tune TBERT-BiLSTM-CRF 特征融合
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BERT-TECNN模型的文本分类方法研究 被引量:19
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作者 李铁飞 生龙 吴迪 《计算机工程与应用》 CSCD 北大核心 2021年第18期186-193,共8页
由于Bert-base,Chinese预训练模型参数巨大,在做分类任务微调时内部参数变化较小,易产生过拟合现象,泛化能力弱,且该模型是以字为单位进行的预训练,包含词信息量较少。针对这些问题,提出了BERT-TECNN模型,模型使用Bert-base,Chinese模... 由于Bert-base,Chinese预训练模型参数巨大,在做分类任务微调时内部参数变化较小,易产生过拟合现象,泛化能力弱,且该模型是以字为单位进行的预训练,包含词信息量较少。针对这些问题,提出了BERT-TECNN模型,模型使用Bert-base,Chinese模型作为动态字向量模型,输出包含深度特征信息的字向量,Transformerencoder层再次对数据进行多头自注意力计算,提取特征信息,以提高模型的泛化能力,CNN层利用不同大小卷积核,捕捉每条数据中不同长度词的信息,最后应用softmax进行分类。该模型与Word2Vec+CNN、Word2Vec+BiLSTM、Elmo+CNN、BERT+CNN、BERT+BiLSTM、BERT+Transformer等深度学习文本分类模型在三种数据集上进行对比实验,得到的准确率、精确率、召回率、F1测度值均为最高。实验表明该模型有效地提取了文本中字词的特征信息,优化了过拟合问题,提高了泛化能力。 展开更多
关键词 bert transformer ENCODER CNN 文本分类 fine-tuning self-attention 过拟合
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仓储环境下基于深度学习的物体识别方法研究 被引量:3
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作者 金秋 李天剑 《北京信息科技大学学报(自然科学版)》 2018年第1期60-65,共6页
针对仓储环境下叉车机器人物体识别的应用场景,提出一种基于Faster-RCNN优化和改进后的物体识别算法。通过对Faster-RCNN模型进行微调(fine-tuning),完成对托盘、货物、人以及叉车等物体的识别,同时优化了训练过程,使得网络最后达到最... 针对仓储环境下叉车机器人物体识别的应用场景,提出一种基于Faster-RCNN优化和改进后的物体识别算法。通过对Faster-RCNN模型进行微调(fine-tuning),完成对托盘、货物、人以及叉车等物体的识别,同时优化了训练过程,使得网络最后达到最优。同时通过对不同共享卷积层模型下的Faster-RCNN进行比较,最后得到最优的Faster-RCNN模型为ZF+RPN模型。实验表明,改进和优化后的算法对仓储环境下的物体检测的准确率达到90%,测试的帧率为33.3fps,基本满足叉车机器人对物体检测实时性和准确性的要求。 展开更多
关键词 深度学习 Faster-RCNN fine-tuning RPN模型 共享卷积层
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基于小波包与CNN的滚动轴承故障诊断 被引量:10
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作者 许理 李戈 +1 位作者 余亮 姚毅 《四川理工学院学报(自然科学版)》 CAS 2018年第3期54-59,共6页
滚动轴承的振动信号具有较强的非平稳性,小波包(Wavelet Packet,WP)时频分析方法能有效提取非平稳信号的时频特征,具有精细的时频分辨率。而卷积神经网络(Convolutional Neural Network,CNN)强大的特征学习能力使其具有优于浅层网络的... 滚动轴承的振动信号具有较强的非平稳性,小波包(Wavelet Packet,WP)时频分析方法能有效提取非平稳信号的时频特征,具有精细的时频分辨率。而卷积神经网络(Convolutional Neural Network,CNN)强大的特征学习能力使其具有优于浅层网络的故障识别率。为了更准确地诊断出滚动轴承的运行状态,提出一种基于小波包与CNN相结合的滚动轴承故障诊断方法:对采集的轴承振动信号进行小波包时频分析,得到各类信号的时频特征图,采用fine-tuning技术在CNN模型caffe Net上进行微调,解决少量样本训练CNN模型的问题,最终得到了可用于滚动轴承故障诊断的CNN模型。采用小波包与CNN相结合进行故障诊断,故障识别率达到了99.1%,高于连续小波变换(CWT)和短时傅里叶变换(STFT)与CNN相结合的故障识别率。而采用主成分分析(PCA)与支持向量机(SVM)相结合的故障识别率最低,且对复合故障的识别效果明显不足。 展开更多
关键词 滚动轴承 小波包 卷积神经网络 故障诊断 fine-tuning技术
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基于卷积神经网络的脚部关键参数计算方法 被引量:3
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作者 梁志剑 常力丹 谢红宇 《科学技术与工程》 北大核心 2019年第6期190-195,共6页
针对传统制鞋业定制化程度低、无法适应足部多样性及舒适性,提出了一种基于卷积神经网络的脚型关键参数计算方法。首先对图像进行透视变换等预处理;然后使用fine-tune的迁移学习方法,通过修改VGG(visual geometry group)神经网络源模型... 针对传统制鞋业定制化程度低、无法适应足部多样性及舒适性,提出了一种基于卷积神经网络的脚型关键参数计算方法。首先对图像进行透视变换等预处理;然后使用fine-tune的迁移学习方法,通过修改VGG(visual geometry group)神经网络源模型全连接分类层,将高层卷积权重进行微调;优化网络模型并提取特征值进行特征分类,从图像中识别出脚的轮廓。最后通过设计的算法计算出脚型特征值;并与实际测量的脚长、腰窝宽度、脚宽等做对比。实验表明,改进后的模型对脚部识别的准确率达到96. 8%,输出结果与测量的真实数据相比误差不超过3%,可作为鞋底制作的重要依据。 展开更多
关键词 脚型特征 深度学习 卷积神经网络 迁移学习 fine-tune 形态学算法
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基于迁移学习的图像识别研究 被引量:11
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作者 袁文翠 孔雪 《微型电脑应用》 2018年第7期10-12,共3页
在运用深度学习解决问题时,最常见障碍在于训练模型需要海量的数据。虽然每天互联网上以TB的量级产生数据(尤其是无语言障碍的图像数据),但对于新领域仅有小部分的数据带有标签,若对这些数据都进行人工标记,将会耗费大量的人力与物力,... 在运用深度学习解决问题时,最常见障碍在于训练模型需要海量的数据。虽然每天互联网上以TB的量级产生数据(尤其是无语言障碍的图像数据),但对于新领域仅有小部分的数据带有标签,若对这些数据都进行人工标记,将会耗费大量的人力与物力,这就给模型的训练带来了极大的挑战。将针对上述问题,提出运用Fine-tune模型、域对抗训练从现有的数据中迁移知识,训练自己的小样本数据集。 展开更多
关键词 深度学习 fine-tune模型 域对抗
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深度卷积神经网络嵌套fine-tune的图像美感品质评价 被引量:3
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作者 李雨鑫 普园媛 +2 位作者 徐丹 钱文华 刘和娟 《山东大学学报(工学版)》 CAS 北大核心 2018年第3期60-66,共7页
针对使用卷积神经网络对图像美感品质研究中图像数据库过小的问题,使用fine-tune的迁移学习方法,分析卷积神经网络结构和图像内容对图像美感品质评价的影响。在按图像内容进行美感品质评价研究时,针对图像数据再次减小的问题,提出连续两... 针对使用卷积神经网络对图像美感品质研究中图像数据库过小的问题,使用fine-tune的迁移学习方法,分析卷积神经网络结构和图像内容对图像美感品质评价的影响。在按图像内容进行美感品质评价研究时,针对图像数据再次减小的问题,提出连续两次fine-tune的嵌套fine-tune方法,并在数据库Photo Quality上进行试验。试验结果表明,嵌套fine-tune方法得到的美感品质评价正确率比传统提取人工设计特征方法平均高出5.36%,比两种深度学习方法分别平均高出3.35%和2.33%,有效解决了卷积神经网络在图像美感品质研究中因图像数据库过小而带来的训练问题。 展开更多
关键词 图像美感品质评价 图像内容 CNN 迁移学习 嵌套fine-tune
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Toward fine-tuned metabolic networks in industrial microorganisms 被引量:1
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作者 Ning Li Weizhu Zeng +1 位作者 Sha Xu Jingwen Zhou 《Synthetic and Systems Biotechnology》 SCIE 2020年第2期81-91,共11页
There are numerous microorganisms in nature capable of synthesizing diverse useful compounds;however,these natural microorganisms are generally inefficient in the production of target products on an industrial scale,r... There are numerous microorganisms in nature capable of synthesizing diverse useful compounds;however,these natural microorganisms are generally inefficient in the production of target products on an industrial scale,relative to either chemical synthesis or extraction methods.To achieve industrial production of useful compounds,these natural microorganisms must undergo a certain degree of mutation or effective fine-tuning strategies.This review describes how to achieve an ideal metabolic fine-tuned process,including static control strategies and dynamic control strategies.The static control strategies mainly focus on various matabolic engineering strategies,including protein engineering,upregulation/downregulation,and combinatrorial control of these metabolic engineering strategies,to enhance the flexibility of their application in fine-tuned metabolic metworks.Then,we focus on the dynamic control strategies for fine-tuned metabolic metworks.The design principles derived would guide us to construct microbial cell factories for various useful compounds. 展开更多
关键词 fine-tuned regulation Protein engineering UPREGULATION DOWNREGULATION Dynamic regulation
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The RLCK-VND6 module coordinates secondary cell wall formation and adaptive growth in rice
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作者 Shaoxue Cao Yan Wang +6 位作者 Yihong Gao Rui Xu Jianing Ma Zuopeng Xu Keke Shang-Guan Baocai Zhang Yihua Zhou 《Molecular Plant》 SCIE CSCD 2023年第6期999-1015,共17页
The orderly deposition of secondary cell wall(SCW)in plants is implicated in various biological programs and is precisely controlled.Although many positive and negative regulators of SCW have been documented,the molec... The orderly deposition of secondary cell wall(SCW)in plants is implicated in various biological programs and is precisely controlled.Although many positive and negative regulators of SCW have been documented,the molecular mechanisms underlying SCW formation coordinated with distinct cellular physiological processes during plant adaptive growth remain largely unclear.Here,we report the identification of Cellulose Synthase co-expressed Kinase1(CSK1),which encodes a receptor-like cytoplasmic kinase,as a negative regulator of SCW formation and its signaling cascade in rice.Transcriptome deep sequencing of developing internodes and genome-wide co-expression assays revealed that CSK1 is co-expressed with cellulose synthase genes and is responsive to various stress stimuli.The increased SCW thickness and vigorous vessel transport in csk1 indicate that CSK1 functions as a negative regulator of SCW biosynthesis.Through observation of green fluorescent protein-tagged CSK1 in rice protoplasts and stable transgenic plants,we found that CSK1 is localized in the nucleus and cytoplasm adjacent to the plasma membrane.Biochemical and molecular assays demonstrated that CSK1 phosphorylates VASCULAR-RELATED NAC-DOMAIN 6(VND6),a master SCW-associated transcription factor,in the nucleus,which reduces the transcription of a suite of SCW-related genes,thereby attenuating SCW accumulation.Consistently,genetic analyses show that CSK1 functions upstream of VND6 in regulating SCW formation.Interestingly,our physiological analyses revealed that CSK1 and VND6 are involved in abscisic acid-mediated regulation of cell growth and SCW deposition.Taken together,these results indicate that the CSK1-VND6 module is an important component of the SCW biosynthesis machinery,which coordinates SCW accumulation and adaptive growth in rice.Our study not only identifies a new regulator of SCW biosynthesis but also reveals a fine-tuned mechanism for precise control of SCW deposition,offering tools for rationally tailoring agronomic traits. 展开更多
关键词 RLCK signaling KINASE fine-tuning regulator secondary cell wall biosynthesis mechanical force ABA adaptation
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NetPrune:A sparklines visualization for network pruning
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作者 Luc-Etienne Pommé Romain Bourqui +2 位作者 Romain Giot Jason Vallet David Auber 《Visual Informatics》 EI 2023年第2期85-99,共15页
Current deep learning approaches are cutting-edge methods for solving classification tasks.Arising transfer learning techniques allows applying large generic model to simple tasks whereas simpler models could be used.... Current deep learning approaches are cutting-edge methods for solving classification tasks.Arising transfer learning techniques allows applying large generic model to simple tasks whereas simpler models could be used.Large models raise the major problem of their memory consumption and processor usage and lead to a prohibitive ecological footprint.In that paper,we present a novel visual analytics approach to interactively prune those networks and thus limit that issue.Our technique leverages a novel sparkline matrix visualization technique as well as a novel local metric which evaluates the discriminatory power of a filter to guide the pruning process and make it interpretable.We assess the well-founded of our approach through two realistic case studies and a user study.For both of them,the interactive refinement of the model led to a significantly smaller model having similar prediction accuracy than the original one. 展开更多
关键词 Explainable pruning Guided fine-tuning VISUALIZATION Deep learning
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