This paper established a numerical model for a solid oxide fuel cell （SOFC） button cell, focusing on the effects of finger-like channels on the gas transport process in the anode support. The current densities of ch...This paper established a numerical model for a solid oxide fuel cell （SOFC） button cell, focusing on the effects of finger-like channels on the gas transport process in the anode support. The current densities of channelled button cell and un-channelled button cell are compared at different operating temperature and voltage with H2 as the fuel. The H2 transport is discussed in detail, such as the mole fraction distribution of H2 in the porous layer, the diffusion flux and convective flux of Ha. It is found that the performance of SOFC can be improved by 2.60 % at 800 ~C, 0.5 V, com- pared with un-channelled SOFC due to the improved gas transport by the finger-like channels. Then, the model is further extended to study 2D-planar SOFC fuelled with syngas. The mole fraction gradients of H2, CO, CH4 and CO are all substantially reduced by the finger-like channels compared to un-channelled planar cell. It is found that the SOFC performance is improved by 5.93 % at 800℃, 0.5 V, when syngas fuel is used. The present study clearly demonstrated that the use of finger-like channels in the anode support is effective in improving the gas transport and the SOFC performance. The present model can be employed for subsequent optimization of the channel configuration for further performance improvement.展开更多
利用生物信息学数据库分析泛素样含PHD和环指结构域蛋白1(UHRF1)在恶性胸膜间皮瘤(MPM)中的表达水平及临床意义。基于TCGA数据库和GTEx数据库差异表达分析UHRF1 m RNA在MPM组织和正常肺组织中的表达水平;使用R软件分析UHRF1 mRNA表达量...利用生物信息学数据库分析泛素样含PHD和环指结构域蛋白1(UHRF1)在恶性胸膜间皮瘤(MPM)中的表达水平及临床意义。基于TCGA数据库和GTEx数据库差异表达分析UHRF1 m RNA在MPM组织和正常肺组织中的表达水平;使用R软件分析UHRF1 mRNA表达量与临床病理参数的相关性;构建Kaplan-Meier模型和单因素多因素COX回归模型分析UHRF1基因在MPM中的预后;利用TIMER2.0数据库分析UHRF1基因与免疫细胞浸润的关系;GSEA分析UHRF1基因发挥功能的主要富集通路。选取8例MPM组织及4例非MPM胸膜组织,通过RT-q PCR的方法验证UHRF1在MPM与非MPM胸膜组织的表达情况。数据库分析结果表明,与正常肺组织相比,UHRF1 m RNA在MPM组织中高表达;UHRF1高表达患者提示MPM患者预后不良;UHRF1基因表达量与CD4^(+)辅助型T细胞2、CD4^(+)效应记忆性T细胞、巨噬细胞等多种免疫细胞浸润水平具有显著的相关性(P<0.01),且显著影响MPM患者的预后。功能富集分析显示,UHRF1主要在DNA复制、蛋白酶体、同源重组等通路中起作用。在收集到的病例样本中,与非MPM胸膜组织相比,UHRF1 mRNA在MPM组织中的表达显著增高(P<0.001)。UHRF1在MPM组织中高表达,可能通过调节DNA甲基化和免疫细胞浸润来影响MPM患者预后,有望成为MPM治疗和预后评估的潜在靶点。展开更多
Sp1/Krüppel样因子(Sp1-like and Krüppel-like factors,Sp1/KLFs)是一组与真核细胞转录调控密切相关的锌指蛋白。Sp1/KLFs的羧基末端高度保守,含有3个串联的Cys2His2锌指结构,用于结合DNA;其氨基末端在不同的家族成员间存在...Sp1/Krüppel样因子(Sp1-like and Krüppel-like factors,Sp1/KLFs)是一组与真核细胞转录调控密切相关的锌指蛋白。Sp1/KLFs的羧基末端高度保守,含有3个串联的Cys2His2锌指结构,用于结合DNA;其氨基末端在不同的家族成员间存在较大差异,主要是通过结合辅助因子发挥转录调控作用。Sp1/KLFs的表达具有组织、细胞分布以及发育时期的特异性,它们通过调控多种富含GC或CACCC的启动子的基因的表达,参与细胞增殖、分化、凋亡和肿瘤发生、发展等多种生理、病理过程。文章综述了Sp1/KLFs的结构特征、作用机制及生物学功能。展开更多
基金supported by a Grant(PolyU 152127/14E) from Research Grant Council,University Grants Committee,Hong Kong SAR
文摘This paper established a numerical model for a solid oxide fuel cell （SOFC） button cell, focusing on the effects of finger-like channels on the gas transport process in the anode support. The current densities of channelled button cell and un-channelled button cell are compared at different operating temperature and voltage with H2 as the fuel. The H2 transport is discussed in detail, such as the mole fraction distribution of H2 in the porous layer, the diffusion flux and convective flux of Ha. It is found that the performance of SOFC can be improved by 2.60 % at 800 ~C, 0.5 V, com- pared with un-channelled SOFC due to the improved gas transport by the finger-like channels. Then, the model is further extended to study 2D-planar SOFC fuelled with syngas. The mole fraction gradients of H2, CO, CH4 and CO are all substantially reduced by the finger-like channels compared to un-channelled planar cell. It is found that the SOFC performance is improved by 5.93 % at 800℃, 0.5 V, when syngas fuel is used. The present study clearly demonstrated that the use of finger-like channels in the anode support is effective in improving the gas transport and the SOFC performance. The present model can be employed for subsequent optimization of the channel configuration for further performance improvement.
文摘利用生物信息学数据库分析泛素样含PHD和环指结构域蛋白1(UHRF1)在恶性胸膜间皮瘤(MPM)中的表达水平及临床意义。基于TCGA数据库和GTEx数据库差异表达分析UHRF1 m RNA在MPM组织和正常肺组织中的表达水平;使用R软件分析UHRF1 mRNA表达量与临床病理参数的相关性;构建Kaplan-Meier模型和单因素多因素COX回归模型分析UHRF1基因在MPM中的预后;利用TIMER2.0数据库分析UHRF1基因与免疫细胞浸润的关系;GSEA分析UHRF1基因发挥功能的主要富集通路。选取8例MPM组织及4例非MPM胸膜组织,通过RT-q PCR的方法验证UHRF1在MPM与非MPM胸膜组织的表达情况。数据库分析结果表明,与正常肺组织相比,UHRF1 m RNA在MPM组织中高表达;UHRF1高表达患者提示MPM患者预后不良;UHRF1基因表达量与CD4^(+)辅助型T细胞2、CD4^(+)效应记忆性T细胞、巨噬细胞等多种免疫细胞浸润水平具有显著的相关性(P<0.01),且显著影响MPM患者的预后。功能富集分析显示,UHRF1主要在DNA复制、蛋白酶体、同源重组等通路中起作用。在收集到的病例样本中,与非MPM胸膜组织相比,UHRF1 mRNA在MPM组织中的表达显著增高(P<0.001)。UHRF1在MPM组织中高表达,可能通过调节DNA甲基化和免疫细胞浸润来影响MPM患者预后,有望成为MPM治疗和预后评估的潜在靶点。
文摘Sp1/Krüppel样因子(Sp1-like and Krüppel-like factors,Sp1/KLFs)是一组与真核细胞转录调控密切相关的锌指蛋白。Sp1/KLFs的羧基末端高度保守,含有3个串联的Cys2His2锌指结构,用于结合DNA;其氨基末端在不同的家族成员间存在较大差异,主要是通过结合辅助因子发挥转录调控作用。Sp1/KLFs的表达具有组织、细胞分布以及发育时期的特异性,它们通过调控多种富含GC或CACCC的启动子的基因的表达,参与细胞增殖、分化、凋亡和肿瘤发生、发展等多种生理、病理过程。文章综述了Sp1/KLFs的结构特征、作用机制及生物学功能。
