We conducted experiments in an artificial stream tank with wild juvenile Chinese sturgeon Acipenser sinensis captured in the Yangtze Estuary to test the null hypothesis that their feeding efficiency on Barcheek goby R...We conducted experiments in an artificial stream tank with wild juvenile Chinese sturgeon Acipenser sinensis captured in the Yangtze Estuary to test the null hypothesis that their feeding efficiency on Barcheek goby Rhinogobius giurinus was not affected by sand vs. smooth glass substrate. Gobies are among the most common prey eaten by wild juvenile A. sinensis in the estuary. Test results found neither substrate type significantly affected feeding efficiency by juveniles. Previous research found a strong innate habitat preference of A. sinensis for sand substrate. The present results indicate that the preference for sand is not related to efficiently capturing R. giurinus on sand, but is an adaptation predisposing juvenile A. sinensis to seek sandy substrate where R. giurinus and other benthic forage are the most abundant in the Yangtze Estuary.展开更多
[ Objective] The aim was to predict the secondary structure and B cell epitope of growth hormone （GH） protein from Acipenser sinensis. [Method] With the amino acid sequence of GH protein from A. sinensis as the base...[ Objective] The aim was to predict the secondary structure and B cell epitope of growth hormone （GH） protein from Acipenser sinensis. [Method] With the amino acid sequence of GH protein from A. sinensis as the base, the secondary structure of GH protein from A. sinensis was predicted by the method of Gamier-Robson, Chou-Fasman and Karpius-Schulz, and its cell epitope was predicted by the method of Kyte- Doolittle, Emini and Jameson-Wolf. [Result] The sections of 18 -23, 55 -55, 67 -73, 83 -86,112 -114,151 -157 and 209 -211 in the N terminal of GH protein molecule had softer structure and these sections could sway or fold to produce more complex tertiary structure. The sections of 19 -23, 51 -71,84 -95, 128 -139, 164 -176 and 189 -195 in the N terminal of GH protein could be the epitope of B cell and there were flexible regions in these sections or near these sections of GH protein molecule. So the dominant regions could be in these sections or near these sections. [ Conclusion] The research provided the basis for the preparation of monoctonal antibody of GH protein from A. sinensis and provided the reference for the discussion for the molecular regulation mechanism of A. sinensis.展开更多
According to the conserved sequence of the ferritin gene, a homologous sequence was obtained from the EST database through a BLAST search against the GenBank database. This sequence was amplified with the method of RT...According to the conserved sequence of the ferritin gene, a homologous sequence was obtained from the EST database through a BLAST search against the GenBank database. This sequence was amplified with the method of RT-PCR, false sequencing was corrected, and full length eDNA of the ferritin subunit from the Chinese sturgeon was obtained. After being submitted to the GenBank database, the sequence accession number EU348782 was assigned. With the length of 896 bp, this eDNA includes entire coding regions of 53 lbp, which encodes 176 amino acids （aa）. The molecular weight was predicted to be 20339.9Mr and the theoretical isoelectric point 5.66. It shares 82.9% protein sequence homology with the ferritin of the Atlantic salmon. This gene is expressed in many organs of the Chinese sturgeon, for example, the liver, pancreas, muscle, brain, heart and gastric mucosa. The highest expression level was found in the pancreas and the heart, while the muscular tissue showed the lowest. Homology modeling was used to predict the 3-D structure of the protein, which included 5 alpha helices and 10 turns. The ferritin protein structure could be overlapped and showed high similarity with that of human, flog and bacteria. It was revealed that this kind of ferritin was highly conserved in structure and function.展开更多
目的 构建一种基于线粒体DNA和核DNA遗传标记的中华鲟及其潜在杂交种的鉴定方法。方法 根据美国国立生物技术信息中心(National Center for Biotechnology Information, NCBI)已公布的中华鲟cytb基因序列设计引物,并选用鲟鱼单拷贝核基...目的 构建一种基于线粒体DNA和核DNA遗传标记的中华鲟及其潜在杂交种的鉴定方法。方法 根据美国国立生物技术信息中心(National Center for Biotechnology Information, NCBI)已公布的中华鲟cytb基因序列设计引物,并选用鲟鱼单拷贝核基因fam43a扩增引物对中华鲟DNA进行扩增与测序,其中对fam43a基因扩增产物进行TA克隆测序。通过BLAST序列相似性检索确定样本来源,并基于邻接法构建鲟形目系统进化树。结果 中华鲟样本测序所得cytb和fam43a基因序列长度分别为728 bp和730 bp,且10个克隆群所测fam43a基因序列完全一致。基本局部比对搜索工具(basic local alignment search tool, BLAST)序列相似分析显示,所测cytb与fam43a基因序列均与GenBank数据库中的中华鲟相似性最高,相似性分别为99.86%和100%。系统进化分析显示,中华鲟样本所测得序列均与GenBank数据库中的中华鲟DNA序列聚类在一起,且与鲟形目其他物种位于不同分支。结论 联合使用线粒体DNA和核DNA遗传标记能够快速准确地鉴定中华鲟及其潜在杂交种,该方法能够满足法医日常检案的鉴定需求,为中华鲟的拯救和保护提供科学技术支持。展开更多
As a living fossil, the endangered Chinese sturgeon(Acipenser sinensis) has been considered a national treasure in China. Here,the famous Gezhouba Dam and Three Gorges Dam on the Yangtze River were built in 1988 and 2...As a living fossil, the endangered Chinese sturgeon(Acipenser sinensis) has been considered a national treasure in China. Here,the famous Gezhouba Dam and Three Gorges Dam on the Yangtze River were built in 1988 and 2006, for economic purposes.The natural population of Chinese sturgeon has declined since then, as these dams block its migratory route to the original spawning grounds in the middle reaches of the Yangtze River. In 2013 and 2014, there was an absence of spawning where it typically happened near the Gezhouba Dam. Nevertheless, from April to June in 2015, over 1,000 larvae with different body lengths(10–35 cm) were detected along the Shanghai Yangtze Estuary; but only little is currently known about the population genetic structure of the Chinese sturgeon. Herein, we inferred population genetic parameters from 462 available Chinese sturgeon specimens based on a 421-bp fragment of the mitochondrial DNA(mtDNA) D-loop region and 1,481,620 SNPs(singlenucleotide polymorphisms) generated by restriction site-associated DNA sequencing(RAD-seq). For the D-loop dataset, 15 haplotypes were determined. Randomly picked 23 individuals, representing the 15 D-loop haplotype groups, were subsequently used for further RAD-seq validation. The average nucleotide diversity calculated from the mtDNA and RAD datasets was 0.0086 and 0.000478, respectively. The overall effective female population size was calculated to be 1,255 to 2,607, and the long-term effective population size was estimated to range from 11,950 to 119,500. We observed that the genetic variability and the effective female population size of the current population in the Yangtze River are severely low, which are similar to the data reported over 10 years ago. The deduced relatively small effective population of female fish, limiting the genetic connectivity among Chinese sturgeon, should be considered a serious threat to this endangered species.展开更多
文摘We conducted experiments in an artificial stream tank with wild juvenile Chinese sturgeon Acipenser sinensis captured in the Yangtze Estuary to test the null hypothesis that their feeding efficiency on Barcheek goby Rhinogobius giurinus was not affected by sand vs. smooth glass substrate. Gobies are among the most common prey eaten by wild juvenile A. sinensis in the estuary. Test results found neither substrate type significantly affected feeding efficiency by juveniles. Previous research found a strong innate habitat preference of A. sinensis for sand substrate. The present results indicate that the preference for sand is not related to efficiently capturing R. giurinus on sand, but is an adaptation predisposing juvenile A. sinensis to seek sandy substrate where R. giurinus and other benthic forage are the most abundant in the Yangtze Estuary.
文摘[ Objective] The aim was to predict the secondary structure and B cell epitope of growth hormone （GH） protein from Acipenser sinensis. [Method] With the amino acid sequence of GH protein from A. sinensis as the base, the secondary structure of GH protein from A. sinensis was predicted by the method of Gamier-Robson, Chou-Fasman and Karpius-Schulz, and its cell epitope was predicted by the method of Kyte- Doolittle, Emini and Jameson-Wolf. [Result] The sections of 18 -23, 55 -55, 67 -73, 83 -86,112 -114,151 -157 and 209 -211 in the N terminal of GH protein molecule had softer structure and these sections could sway or fold to produce more complex tertiary structure. The sections of 19 -23, 51 -71,84 -95, 128 -139, 164 -176 and 189 -195 in the N terminal of GH protein could be the epitope of B cell and there were flexible regions in these sections or near these sections of GH protein molecule. So the dominant regions could be in these sections or near these sections. [ Conclusion] The research provided the basis for the preparation of monoctonal antibody of GH protein from A. sinensis and provided the reference for the discussion for the molecular regulation mechanism of A. sinensis.
文摘According to the conserved sequence of the ferritin gene, a homologous sequence was obtained from the EST database through a BLAST search against the GenBank database. This sequence was amplified with the method of RT-PCR, false sequencing was corrected, and full length eDNA of the ferritin subunit from the Chinese sturgeon was obtained. After being submitted to the GenBank database, the sequence accession number EU348782 was assigned. With the length of 896 bp, this eDNA includes entire coding regions of 53 lbp, which encodes 176 amino acids （aa）. The molecular weight was predicted to be 20339.9Mr and the theoretical isoelectric point 5.66. It shares 82.9% protein sequence homology with the ferritin of the Atlantic salmon. This gene is expressed in many organs of the Chinese sturgeon, for example, the liver, pancreas, muscle, brain, heart and gastric mucosa. The highest expression level was found in the pancreas and the heart, while the muscular tissue showed the lowest. Homology modeling was used to predict the 3-D structure of the protein, which included 5 alpha helices and 10 turns. The ferritin protein structure could be overlapped and showed high similarity with that of human, flog and bacteria. It was revealed that this kind of ferritin was highly conserved in structure and function.
基金supported by National Natural Science Foundation of China (31370047, U1301252)Shenzhen Scientific R & D Grant (GJHS20160331150703934)+1 种基金Shenzhen Dapeng Special Program for Industrial Development (KY20160102)Zhenjiang Leading Talent Program for Innovation and Entrepreneurship
文摘As a living fossil, the endangered Chinese sturgeon(Acipenser sinensis) has been considered a national treasure in China. Here,the famous Gezhouba Dam and Three Gorges Dam on the Yangtze River were built in 1988 and 2006, for economic purposes.The natural population of Chinese sturgeon has declined since then, as these dams block its migratory route to the original spawning grounds in the middle reaches of the Yangtze River. In 2013 and 2014, there was an absence of spawning where it typically happened near the Gezhouba Dam. Nevertheless, from April to June in 2015, over 1,000 larvae with different body lengths(10–35 cm) were detected along the Shanghai Yangtze Estuary; but only little is currently known about the population genetic structure of the Chinese sturgeon. Herein, we inferred population genetic parameters from 462 available Chinese sturgeon specimens based on a 421-bp fragment of the mitochondrial DNA(mtDNA) D-loop region and 1,481,620 SNPs(singlenucleotide polymorphisms) generated by restriction site-associated DNA sequencing(RAD-seq). For the D-loop dataset, 15 haplotypes were determined. Randomly picked 23 individuals, representing the 15 D-loop haplotype groups, were subsequently used for further RAD-seq validation. The average nucleotide diversity calculated from the mtDNA and RAD datasets was 0.0086 and 0.000478, respectively. The overall effective female population size was calculated to be 1,255 to 2,607, and the long-term effective population size was estimated to range from 11,950 to 119,500. We observed that the genetic variability and the effective female population size of the current population in the Yangtze River are severely low, which are similar to the data reported over 10 years ago. The deduced relatively small effective population of female fish, limiting the genetic connectivity among Chinese sturgeon, should be considered a serious threat to this endangered species.
