水稻剑叶性状与产量息息相关,也是水稻遗传学和分子生物学研究的热点之一。剖析水稻剑叶长度的遗传机理,为培育理想株型新品种、提高水稻产量奠定基础。以长剑叶的非洲长雄蕊野生稻(Oryza longistaminata A. Chev.&Roehr.)为父本、...水稻剑叶性状与产量息息相关,也是水稻遗传学和分子生物学研究的热点之一。剖析水稻剑叶长度的遗传机理,为培育理想株型新品种、提高水稻产量奠定基础。以长剑叶的非洲长雄蕊野生稻(Oryza longistaminata A. Chev.&Roehr.)为父本、短剑叶的粳稻Balilla为母本构建F2分离群体。统计2015-2017年近3年的F2群体的表型数据和基因型数据,对剑叶长度性状进行了遗传效应分析和QTL定位。一共检测到10个控制剑叶长度的QTL位点,分布在水稻的第3、4、5、7、8、9、11和12号染色体上。其中位于第9号染色体上的q FLL9-1是群体中控制水稻剑叶长度的主效位点,还鉴定到了两个新位点qFLL4-1和qFLL11-1。针对主效位点q FLL9-1构建了高世代回交群体BC6F2,验证了qFLL9-1在群体中发挥重要的效应。本研究鉴定到qFLL4-1和qFLL11-1两个调控剑叶长度的新QTL位点,验证了1个调控水稻剑叶长度的主效QTL qFLL9-1,研究结果为水稻剑叶长度QTL的精细定位奠定了基础,有利于长雄蕊野生稻种质资源的进一步开发与利用。展开更多
A doubled haploid （DH） population, which consists of 120 lines derived from anther culture of a typical indica and japonica hybrid‘CJ06＇/‘TNI＇, was used in this study. Ligule lengths of flag leaf were investigat...A doubled haploid （DH） population, which consists of 120 lines derived from anther culture of a typical indica and japonica hybrid‘CJ06＇/‘TNI＇, was used in this study. Ligule lengths of flag leaf were investigated for quantitative trait loci （QTL） mapping using the DH population. Five QTLs （qLL-2, qLL.4, qLL-6, qLL-IO and qLL-12） controlling the ligule length （LL） were detected on chromosomes 2, 4, 6, 10 and 12, with the variances explained 11.4%, 13.6%, 27.8%, 22.1% and 11.0%, respectively. Using four known genes of ZmGL1, ZmGL2, ZmGL3 and ZmGL4 in maize from the MaizeGDB, their homologs in rice were aligned and integrated into the existing simple sequence repeats linkage map by in silico mapping. A ZmLG1 homolog gene, OsLG1 encoding a squamosa promoter binding protein, was located between the markers RM255 and RM280, which is just identical to the interval of qLL.4 on the long arm of chromosome 4. The results are beneficial to dissection of the ligule molecular mechanism and the study of cereal evolution.展开更多
基金Supported by the State Key Basic Research and Development Plan ofChina(2007CB10920203)the National Natural Science Foundation of China(30771160,30571136)+1 种基金the Open Fund of State Key Laboratory of RiceBiology(080104)the Research Program of Zhejiang Province.
文摘A doubled haploid （DH） population, which consists of 120 lines derived from anther culture of a typical indica and japonica hybrid‘CJ06＇/‘TNI＇, was used in this study. Ligule lengths of flag leaf were investigated for quantitative trait loci （QTL） mapping using the DH population. Five QTLs （qLL-2, qLL.4, qLL-6, qLL-IO and qLL-12） controlling the ligule length （LL） were detected on chromosomes 2, 4, 6, 10 and 12, with the variances explained 11.4%, 13.6%, 27.8%, 22.1% and 11.0%, respectively. Using four known genes of ZmGL1, ZmGL2, ZmGL3 and ZmGL4 in maize from the MaizeGDB, their homologs in rice were aligned and integrated into the existing simple sequence repeats linkage map by in silico mapping. A ZmLG1 homolog gene, OsLG1 encoding a squamosa promoter binding protein, was located between the markers RM255 and RM280, which is just identical to the interval of qLL.4 on the long arm of chromosome 4. The results are beneficial to dissection of the ligule molecular mechanism and the study of cereal evolution.
