This paper presents a simulation model based on the finite element method. The method is used to analyze the motion response and mooring line tension of the flatfish cage system in waves. The cage system consists of t...This paper presents a simulation model based on the finite element method. The method is used to analyze the motion response and mooring line tension of the flatfish cage system in waves. The cage system consists of top frames, netting, mooring lines, bottom frames, and floats. A series of scaled physical model tests in regular waves are conducted to verify the numerical model. The comparison results show that the simulated and the experimental results agree well under the wave conditions, and the maximum pitch of the bottom frame with two orientations is about 12o. The motion process of the whole cage system in the wave can be described with the computer visualized technology. Then, the mooring line tensions and the motion of the bottom frame with three kinds of weight are calculated under different wave conditions. According to the numerical results, the differences in mooring line tensions of flatfish cages with three weight modes are indistinct. The maximum pitch of the bottom frame decreases with the increase of the bottom weight.展开更多
A numerical model of flatfish cage is built based on the lumped mass method and the principle of rigid body kinematics. To validate the numerical model, a series of physical model tests are conducted in the wave flume...A numerical model of flatfish cage is built based on the lumped mass method and the principle of rigid body kinematics. To validate the numerical model, a series of physical model tests are conducted in the wave flume. The numerical results correspond well with the data sets from physical model test. The effect of weight of bottom frame, height of fish net and net shape on motion responses of fish cage and tension force on mooring lines is then analyzed. The results indicate that the vertical displacements of float collar and bottom frame decrease with the increase in the weight of bottom frame; the maximum tension force on mooring lines increases with the increasing weight of bottom frame. The inclination angles of float collar and bottom frame decrease with the increasing net height; the maximum tension force increases obviously with the increase of net height.展开更多
Objective:To investigate the surface architecture of the asymmetrical buccal cavity of Solea solea which are considered one of the most important predators in benthic communities.Methods:Adult Solea solea were obtaine...Objective:To investigate the surface architecture of the asymmetrical buccal cavity of Solea solea which are considered one of the most important predators in benthic communities.Methods:Adult Solea solea were obtained from Mediterranean Sea near Damielta.The heads were removed and processed for scanning electron microscopy.Its buccal cavity is asymmetrical and divided into roof and floor and the tongue for histological studies.Results:The buccal cavity roof is formed from upper jaw,velum and the palate.The upper jaw has several wing like processes with teeth arranged in several rows which may help in cutting and pushing the food to the entrance of the digestive canal while the floor is formed from the lower jaw and the tongue.The tongue is divided into apex,body and root.There is a gradual decrease of goblet cells in the tongue from anterior to posterior.These goblet cells function in protection of the epithelium.CoDctusions:Teeth in the floor of the buccal cavity and taste buds can be considered adaptive changes of the oral cavity related to the feeding habits and was a source to identify new and better methods of nutrition in aquaculture of Solea solea.展开更多
The 5’-end of the mitochondrial control region sequences of three flatfishes (Pleuronectiformes: Pleuronectidae) were amplified and sequenced. These sequences were compared with those of other three Pleuronectids spe...The 5’-end of the mitochondrial control region sequences of three flatfishes (Pleuronectiformes: Pleuronectidae) were amplified and sequenced. These sequences were compared with those of other three Pleuronectids species retrieved from GenBank. A phylogenetic tree was constructed based on the partial control region sequences. The results of phyloge- netic analysis are consistent with those of conventional systematics. Compared to previous studies, the structure of the 5’-end of mitochondrial control region was analyzed. The terminal associated sequence motif and its complementary motif were i- dentified at the 5’-end of the sequences. A conserved sequence block, named as CM5’d, was identified in the 5’-end of con- trol region sequences in all Pleuronectids. Another central conserved sequence block, named as CSB-F, was detected in the central conserved blocks.展开更多
Antioxidant peptides of flatfish skin protein hydrolyzed by four enzymes （Papain, Pepsin, Trypsin and Neutrase, respectively）were investigated. The Trypsin hydrotysate obtained by hydrolysis exhibited the highest 1,...Antioxidant peptides of flatfish skin protein hydrolyzed by four enzymes （Papain, Pepsin, Trypsin and Neutrase, respectively）were investigated. The Trypsin hydrotysate obtained by hydrolysis exhibited the highest 1,1- dipheny-l-2-picrylhydrazyl（DPPH） radical scavenging activity （DRSA） compared with other hydrolysates. Re- sponse surface method ology （RSM）, based on Box-Behnken design, was used to study the influence of hydrolysis conditions on the DRSA. The optimal hydrolysis conditions were as follows： pH 7.38, temperature 48.2~C and en- zyme/substrate （E/S）ratio 2 840 U/g. Under these conditions, the maximum DRSA was （22.85 ＋ 0.57）%. The experimental values agreed with the value （23.09%） predicted by the model within a 95% confidence interval. By using gel filtration chromatography and reversed-phase high performance liquid chromatography （RP-HPLC）, anti- oxidant peptide （D2-P）was isolated from flatfish skin protein hydrolysates （FSPH）and could exhibit a （54.28 ± 1.37） % scavenging activity on DPPH radical at the concentration of 5 mg/mL. This is the first report of a scientific basis for the preparation of antioxidant peptides from flatfish skin. The results suggested that the antioxidant pep- tides can be exploited into functional foods or used as a novel source of nutraceuticals.展开更多
Response surface method （RSM）, based on Box-Behnken design, was used to optimize the enzymatic hydrolysis conditions of flatfish skin protein hydrolysates （FSPH）. Among the tested proteases, the combination of nut...Response surface method （RSM）, based on Box-Behnken design, was used to optimize the enzymatic hydrolysis conditions of flatfish skin protein hydrolysates （FSPH）. Among the tested proteases, the combination of nutrase and trypsin was selected. The optimal hydrolysis conditions were as follows： pH 7.3, temperature 51.8℃, and the enzyme/substrate （E/S） ratio 2.5; under these conditions, the maximum peptide yield （PY） was 69.41 =1：0.43%. The physiochemical analysis showed that the amino acids （His, Asp and Glu） of FSPH accounted for 18.15%, and FSPH was a mixture of polypeptides mostly distributed among 900-2000 Da. FSPH could exhibit a 93% chelating effect on ferrous ion at a concentration of 400 gg/mL, and also a notable reducing power. This study showed bioprocess for the production of FSPH for the first time, which had a good potential for valuable ingredients in the food, cosmetic and medicine industries.展开更多
基金financially supported by the Earmarked Fund for Modern Agro-industry Technology Research System(Grant No.CARS-50-G05)the National Natural Science Foundation of China(Grant Nos.31101938+1 种基金30972256 and 51239002)Science and Technology Development Project of Shandong Province(Grant No.2009GG10005005)
文摘This paper presents a simulation model based on the finite element method. The method is used to analyze the motion response and mooring line tension of the flatfish cage system in waves. The cage system consists of top frames, netting, mooring lines, bottom frames, and floats. A series of scaled physical model tests in regular waves are conducted to verify the numerical model. The comparison results show that the simulated and the experimental results agree well under the wave conditions, and the maximum pitch of the bottom frame with two orientations is about 12o. The motion process of the whole cage system in the wave can be described with the computer visualized technology. Then, the mooring line tensions and the motion of the bottom frame with three kinds of weight are calculated under different wave conditions. According to the numerical results, the differences in mooring line tensions of flatfish cages with three weight modes are indistinct. The maximum pitch of the bottom frame decreases with the increase of the bottom weight.
基金supported by the National Natural Science Foundation of China(Grant Nos.51109187,51239002 and 51221961the Project form Zhoushan Science and Technology Bureau(Grant No.2013C41002)
文摘A numerical model of flatfish cage is built based on the lumped mass method and the principle of rigid body kinematics. To validate the numerical model, a series of physical model tests are conducted in the wave flume. The numerical results correspond well with the data sets from physical model test. The effect of weight of bottom frame, height of fish net and net shape on motion responses of fish cage and tension force on mooring lines is then analyzed. The results indicate that the vertical displacements of float collar and bottom frame decrease with the increase in the weight of bottom frame; the maximum tension force on mooring lines increases with the increasing weight of bottom frame. The inclination angles of float collar and bottom frame decrease with the increasing net height; the maximum tension force increases obviously with the increase of net height.
基金funded by Damietta University-Faculty of Science-Zoology Department(Crant No.541113)
文摘Objective:To investigate the surface architecture of the asymmetrical buccal cavity of Solea solea which are considered one of the most important predators in benthic communities.Methods:Adult Solea solea were obtained from Mediterranean Sea near Damielta.The heads were removed and processed for scanning electron microscopy.Its buccal cavity is asymmetrical and divided into roof and floor and the tongue for histological studies.Results:The buccal cavity roof is formed from upper jaw,velum and the palate.The upper jaw has several wing like processes with teeth arranged in several rows which may help in cutting and pushing the food to the entrance of the digestive canal while the floor is formed from the lower jaw and the tongue.The tongue is divided into apex,body and root.There is a gradual decrease of goblet cells in the tongue from anterior to posterior.These goblet cells function in protection of the epithelium.CoDctusions:Teeth in the floor of the buccal cavity and taste buds can be considered adaptive changes of the oral cavity related to the feeding habits and was a source to identify new and better methods of nutrition in aquaculture of Solea solea.
基金the Shandong Foundation of Sciences(No.Y2000D04) the National Key Basic Research Program from the Ministry of Science and Technology of China(No.G19990437).
文摘The 5’-end of the mitochondrial control region sequences of three flatfishes (Pleuronectiformes: Pleuronectidae) were amplified and sequenced. These sequences were compared with those of other three Pleuronectids species retrieved from GenBank. A phylogenetic tree was constructed based on the partial control region sequences. The results of phyloge- netic analysis are consistent with those of conventional systematics. Compared to previous studies, the structure of the 5’-end of mitochondrial control region was analyzed. The terminal associated sequence motif and its complementary motif were i- dentified at the 5’-end of the sequences. A conserved sequence block, named as CM5’d, was identified in the 5’-end of con- trol region sequences in all Pleuronectids. Another central conserved sequence block, named as CSB-F, was detected in the central conserved blocks.
基金Supported by the Key Technology R&D Program of Tianjin(No.14ZCZDNC00008)
文摘Antioxidant peptides of flatfish skin protein hydrolyzed by four enzymes （Papain, Pepsin, Trypsin and Neutrase, respectively）were investigated. The Trypsin hydrotysate obtained by hydrolysis exhibited the highest 1,1- dipheny-l-2-picrylhydrazyl（DPPH） radical scavenging activity （DRSA） compared with other hydrolysates. Re- sponse surface method ology （RSM）, based on Box-Behnken design, was used to study the influence of hydrolysis conditions on the DRSA. The optimal hydrolysis conditions were as follows： pH 7.38, temperature 48.2~C and en- zyme/substrate （E/S）ratio 2 840 U/g. Under these conditions, the maximum DRSA was （22.85 ＋ 0.57）%. The experimental values agreed with the value （23.09%） predicted by the model within a 95% confidence interval. By using gel filtration chromatography and reversed-phase high performance liquid chromatography （RP-HPLC）, anti- oxidant peptide （D2-P）was isolated from flatfish skin protein hydrolysates （FSPH）and could exhibit a （54.28 ± 1.37） % scavenging activity on DPPH radical at the concentration of 5 mg/mL. This is the first report of a scientific basis for the preparation of antioxidant peptides from flatfish skin. The results suggested that the antioxidant pep- tides can be exploited into functional foods or used as a novel source of nutraceuticals.
文摘Response surface method （RSM）, based on Box-Behnken design, was used to optimize the enzymatic hydrolysis conditions of flatfish skin protein hydrolysates （FSPH）. Among the tested proteases, the combination of nutrase and trypsin was selected. The optimal hydrolysis conditions were as follows： pH 7.3, temperature 51.8℃, and the enzyme/substrate （E/S） ratio 2.5; under these conditions, the maximum peptide yield （PY） was 69.41 =1：0.43%. The physiochemical analysis showed that the amino acids （His, Asp and Glu） of FSPH accounted for 18.15%, and FSPH was a mixture of polypeptides mostly distributed among 900-2000 Da. FSPH could exhibit a 93% chelating effect on ferrous ion at a concentration of 400 gg/mL, and also a notable reducing power. This study showed bioprocess for the production of FSPH for the first time, which had a good potential for valuable ingredients in the food, cosmetic and medicine industries.
