INTRODUCTIONAlthough reliable assays for the detection ofhepatitis C virus and E virus became available, still10% 20% hepatitis are not caused byhepatitis A-E virus[1-3]. In 1996, two research groups isolatedthis agen...INTRODUCTIONAlthough reliable assays for the detection ofhepatitis C virus and E virus became available, still10% 20% hepatitis are not caused byhepatitis A-E virus[1-3]. In 1996, two research groups isolatedthis agent independently and almost simultaneouslyand named hepatitis G virus and GB virus C,respectively[4-7].展开更多
Objective: To study the genetic diversity of Culex theileri flavivirus and the spread of this virus among Spain, Portugal and Turkey.Methods: A database consisting of 55 sequences of the NS5/3'UTR region of Culex ...Objective: To study the genetic diversity of Culex theileri flavivirus and the spread of this virus among Spain, Portugal and Turkey.Methods: A database consisting of 55 sequences of the NS5/3'UTR region of Culex theileri flavivirus group downloaded from GenBank were aligned and manual edited with Bioedit.ModelT est v.3.7 was used to select the simplest evolutionary model that adequately fitted the sequence data.Maximum likelihood analysis was performed using MEGA7.The phylogenetic signal of the dataset was investigated by the likelihood mapping analysis.Results: The phylogenetic tree showed three clusters.Myanmar sequences clusterd together with Turkish sequences, Spain and Portugal strains grouped together and two Turkish sequences grouped separately.Selective pressure analysis showed a moderate percentage of sites(22.5%) under pervasive negative selection and only 1% under pervasive positive selection.The sites subject to selective pressure in CTFV RdRp NS5 fragments have been located onto the predicted three-dimensional structure.Conclusions: Phylogenetic and evolutionary analysis can be an important tool for understanding the evolutionary impact of the probable contemporary existence between nonpathogenic and pathogenic flaviviruses among these vectors.展开更多
The outbreak and rapid spread of Zika virus (ZIKV) in the Western Hemisphere has imposed a global public health threat since 2015. ZIKV is a positive-sense single-stranded RNA virus (where the ‘positive-sense’ means...The outbreak and rapid spread of Zika virus (ZIKV) in the Western Hemisphere has imposed a global public health threat since 2015. ZIKV is a positive-sense single-stranded RNA virus (where the ‘positive-sense’ means that the viral RNA can be directly translated by the host cell into viral proteins) that belongs to the genus Flavivirus in the family Flaviviridae.展开更多
Retromer and sorting nexins(SNXs)transport cargoes from endosomes to the trans-Golgi network or plasma membrane.Recent studies have unveiled the emerging roles for retromer and SNXs in the life cycle of viruses,includ...Retromer and sorting nexins(SNXs)transport cargoes from endosomes to the trans-Golgi network or plasma membrane.Recent studies have unveiled the emerging roles for retromer and SNXs in the life cycle of viruses,including members of Coronaviridae,Flaviviridae and Retroviridae.Key components of retromer/SNXs,such as Vps35,Vps26,SNX5 and SNX27,can affect multiple steps of the viral life cycle,including facilitating the entry of viruses into cells,participating in viral replication,and promoting the assembly of virions.Here we present a comprehensive updated review on the interplay between retromer/SNXs and virus,which will shed mechanistic insights into controlling virus infection.展开更多
基金Supported by the Neational Natural Science Foundation of China, No. 39825116, 39970394.
文摘INTRODUCTIONAlthough reliable assays for the detection ofhepatitis C virus and E virus became available, still10% 20% hepatitis are not caused byhepatitis A-E virus[1-3]. In 1996, two research groups isolatedthis agent independently and almost simultaneouslyand named hepatitis G virus and GB virus C,respectively[4-7].
文摘Objective: To study the genetic diversity of Culex theileri flavivirus and the spread of this virus among Spain, Portugal and Turkey.Methods: A database consisting of 55 sequences of the NS5/3'UTR region of Culex theileri flavivirus group downloaded from GenBank were aligned and manual edited with Bioedit.ModelT est v.3.7 was used to select the simplest evolutionary model that adequately fitted the sequence data.Maximum likelihood analysis was performed using MEGA7.The phylogenetic signal of the dataset was investigated by the likelihood mapping analysis.Results: The phylogenetic tree showed three clusters.Myanmar sequences clusterd together with Turkish sequences, Spain and Portugal strains grouped together and two Turkish sequences grouped separately.Selective pressure analysis showed a moderate percentage of sites(22.5%) under pervasive negative selection and only 1% under pervasive positive selection.The sites subject to selective pressure in CTFV RdRp NS5 fragments have been located onto the predicted three-dimensional structure.Conclusions: Phylogenetic and evolutionary analysis can be an important tool for understanding the evolutionary impact of the probable contemporary existence between nonpathogenic and pathogenic flaviviruses among these vectors.
文摘The outbreak and rapid spread of Zika virus (ZIKV) in the Western Hemisphere has imposed a global public health threat since 2015. ZIKV is a positive-sense single-stranded RNA virus (where the ‘positive-sense’ means that the viral RNA can be directly translated by the host cell into viral proteins) that belongs to the genus Flavivirus in the family Flaviviridae.
基金supported by grants from National Natural Science Foundation of China(81871663 and 82072270)Undergraduate Science and Technology Innovation Plan of International Class of Clinical Medicine in Shandong First Medical University(ZYKC2019-016)Academic promotion programme of Shandong First Medical University(2019LJ001)。
文摘Retromer and sorting nexins(SNXs)transport cargoes from endosomes to the trans-Golgi network or plasma membrane.Recent studies have unveiled the emerging roles for retromer and SNXs in the life cycle of viruses,including members of Coronaviridae,Flaviviridae and Retroviridae.Key components of retromer/SNXs,such as Vps35,Vps26,SNX5 and SNX27,can affect multiple steps of the viral life cycle,including facilitating the entry of viruses into cells,participating in viral replication,and promoting the assembly of virions.Here we present a comprehensive updated review on the interplay between retromer/SNXs and virus,which will shed mechanistic insights into controlling virus infection.
