Polybrominated diphenyl ether has become a widespread environmental pollutant in aquatic environments.BDE-209,a prominent PBDEs found in the environment and wildlife,has been proven to cause oxidative stress,immune da...Polybrominated diphenyl ether has become a widespread environmental pollutant in aquatic environments.BDE-209,a prominent PBDEs found in the environment and wildlife,has been proven to cause oxidative stress,immune damage and endocrine system disorders in fish.However,the effects of BDE-209 on the intestinal health of fish remain unclear.This research aimed to determine the effects of BDE-209 on the growth performance,digestive enzymes activities and intestinal microbiota of common carp.After 60-day exposure,the growth performance of common carp were remarkably decreased(P<0.05).Moreover,the levels of protease,amylase and lipase activities remarkably decreased by BDE-209 exposure(P<0.05).In addition,metagenomics analysis revealed that the alpha-diversity values of intestinal microbiota were significantly decreased in BDE-209 exposure groups(P<0.05).The results also indicated that BDE-209 exposure could alter the intestinal microbial compositions.At the phylum level,the proportion of Fusobacteria was markedly reduced(P<0.05),while the proportions of Bacteroidetes and Firmicutes were significantly increased(P<0.05)along with the increasing concentrations of BDE-209.At the genus level,the proportion of Shewanella was higher(P<0.05)in BDE-209 exposure groups than in control group,while the abundance of Cetobacterium showed an opposite trend.Furthermore,the proportions of some aquatic probiotics(such as Bacillus,Rhodopseudomonas and Lactobacillus)were significantly enriched(P<0.05)in control group,and the content of short-chain fatty acid producing bacterium(such as Faecalibacterium,Bacteroides and Clostridium)were decreased along with the increasing BDE-209 concentrations,but no significant differences were found among the four groups(P>0.05).Overall,exposure to BDE-209 could alter the intestinal microbiota of common carp,which may affect their growth and threaten their health.展开更多
为了探究了鲤(Cyprinus carpio)仔稚鱼的生长过程中主要消化酶活性变化规律,实验测定了鲤从孵化出膜到40日龄(日龄,Day after hatching)仔稚鱼期间的生长、可溶性蛋白含量和几种消化酶活性变化。结果显示:仔稚鱼全长以及体重在15日龄后...为了探究了鲤(Cyprinus carpio)仔稚鱼的生长过程中主要消化酶活性变化规律,实验测定了鲤从孵化出膜到40日龄(日龄,Day after hatching)仔稚鱼期间的生长、可溶性蛋白含量和几种消化酶活性变化。结果显示:仔稚鱼全长以及体重在15日龄后增速加快,特定生长率为14.81%。淀粉酶、脂肪酶、胰蛋白酶,糜乳蛋白酶、碱性磷酸酶以及氨基肽酶在1日龄仔鱼体内均能检测到活性。在仔稚鱼发育过程中,其可溶性蛋白含量先下降后上升。仔鱼摄食前消化酶的活性出现一定的上升,随着仔稚鱼消化系统发育以及营养方式的转变,其消化酶活性从3到25日龄处于不断的变化状态,而25日龄到实验结束鲤仔稚鱼的消化酶处于一种相对稳定的状态,标志着其消化功能趋于完善。根据不同发育时期鲤消化酶活性的变化,设计有机可腐化的材料制备人工鱼巢以提高人工鱼巢的生态修复功能,降低饥饿对仔稚鱼成活率的影响。展开更多
基金This work was supported by National Natural Science Foundation of China(31902361,21906046)the Key Technology Research Project of Henan Province(202102110106).
文摘Polybrominated diphenyl ether has become a widespread environmental pollutant in aquatic environments.BDE-209,a prominent PBDEs found in the environment and wildlife,has been proven to cause oxidative stress,immune damage and endocrine system disorders in fish.However,the effects of BDE-209 on the intestinal health of fish remain unclear.This research aimed to determine the effects of BDE-209 on the growth performance,digestive enzymes activities and intestinal microbiota of common carp.After 60-day exposure,the growth performance of common carp were remarkably decreased(P<0.05).Moreover,the levels of protease,amylase and lipase activities remarkably decreased by BDE-209 exposure(P<0.05).In addition,metagenomics analysis revealed that the alpha-diversity values of intestinal microbiota were significantly decreased in BDE-209 exposure groups(P<0.05).The results also indicated that BDE-209 exposure could alter the intestinal microbial compositions.At the phylum level,the proportion of Fusobacteria was markedly reduced(P<0.05),while the proportions of Bacteroidetes and Firmicutes were significantly increased(P<0.05)along with the increasing concentrations of BDE-209.At the genus level,the proportion of Shewanella was higher(P<0.05)in BDE-209 exposure groups than in control group,while the abundance of Cetobacterium showed an opposite trend.Furthermore,the proportions of some aquatic probiotics(such as Bacillus,Rhodopseudomonas and Lactobacillus)were significantly enriched(P<0.05)in control group,and the content of short-chain fatty acid producing bacterium(such as Faecalibacterium,Bacteroides and Clostridium)were decreased along with the increasing BDE-209 concentrations,but no significant differences were found among the four groups(P>0.05).Overall,exposure to BDE-209 could alter the intestinal microbiota of common carp,which may affect their growth and threaten their health.
文摘为了探究了鲤(Cyprinus carpio)仔稚鱼的生长过程中主要消化酶活性变化规律,实验测定了鲤从孵化出膜到40日龄(日龄,Day after hatching)仔稚鱼期间的生长、可溶性蛋白含量和几种消化酶活性变化。结果显示:仔稚鱼全长以及体重在15日龄后增速加快,特定生长率为14.81%。淀粉酶、脂肪酶、胰蛋白酶,糜乳蛋白酶、碱性磷酸酶以及氨基肽酶在1日龄仔鱼体内均能检测到活性。在仔稚鱼发育过程中,其可溶性蛋白含量先下降后上升。仔鱼摄食前消化酶的活性出现一定的上升,随着仔稚鱼消化系统发育以及营养方式的转变,其消化酶活性从3到25日龄处于不断的变化状态,而25日龄到实验结束鲤仔稚鱼的消化酶处于一种相对稳定的状态,标志着其消化功能趋于完善。根据不同发育时期鲤消化酶活性的变化,设计有机可腐化的材料制备人工鱼巢以提高人工鱼巢的生态修复功能,降低饥饿对仔稚鱼成活率的影响。
