[Objectives]To study the effect of total flavonoids extracted from Polygonum perfoliatum L.(TFP)on immune-mediated liver injury induced by bacillus Calmette-Guerin plus lipopolysaccharide(BCG+LPS)in mice,and to explor...[Objectives]To study the effect of total flavonoids extracted from Polygonum perfoliatum L.(TFP)on immune-mediated liver injury induced by bacillus Calmette-Guerin plus lipopolysaccharide(BCG+LPS)in mice,and to explore its action mechanism.[Methods]60 Kunming mice were divided into normal group,model group,control group(bifendate)and TFP low,medium and high dose groups according to random number table method,with 10 mice in each group.On the first day of modeling,mice were injected with 0.2 mL of BCG solution(12.5 mg/mL)through the tail vein,and on the eleventh day,0.2 mL of LPS(37.5μg/mL)were injected into the tail vein to prepare a mouse model of immune-mediated liver injury;from the first day of modeling,the normal group and the model group were administered intragastrically with the corresponding volume of distilled water,and the bifendate group and the TFP high,medium,and low dose groups were administered intragastrically with the corresponding doses once a day for 11 d.After the last time administration,fasting but giving water for 16 h,took blood from eyes,then collected the liver tissue.The levels of alanine transaminase(ALT)and aspartate transaminase(AST)in serum were detected by biochemical method;transforming growth factor-β1(TGF-β1),intercellular adhesion molecule-1(ICAM-1),interleukin-6(IL-6)and interleukin-1β(IL-1β)expression levels in liver tissue were detected by enzyme-linked immunosorbent assay(ELISA);phosphorylated protein tyrosine kinase JAK-2(p-JAK2),phosphorylated signal transducer and activator of transcription 3(p-STAT3)protein expression levels were detected by Western Blot method;the degree of liver tissue lesions was detected by HE staining.[Results]Compared with the model group,the levels of ALT and AST in the serum of mice in each dose group of TFP(high dose 600 mg/kg,medium dose 400 mg/kg,and low dose 200 mg/kg)were reduced,and the activities of T-SOD and GSH-Px were increased;the content or expression ofβ1,ICAM-1,IL-6,IL-1βdecreased,and the expression of p-JAK2 and p-STAT3 protein decreased;pathological sections showed that the degree of inflammatory necrosis and the degree of lesions in the liver tissues of each dose group of TFP were reduced by varying degrees.[Conclusions]TFP has a protective effect on BCG+LPS-induced immune-mediated liver injury in mice.The mechanism may be related to regulating the phosphorylation level of JAK2 and inhibiting the inflammatory reaction,thereby regulating the TGF-β1/STAT3 signaling pathway and improving the immune-mediated liver injury.展开更多
A reliable high performance liquid chromatography method was developed for the quality evaluation of Polygonum aviculare L. Eight marker flavonoids were identified and simultaneously quantified, which included myricit...A reliable high performance liquid chromatography method was developed for the quality evaluation of Polygonum aviculare L. Eight marker flavonoids were identified and simultaneously quantified, which included myricitrin, hyperoside, galuteolin, avicularin, quercitrin, quercetin, luteolin, and kaempferol. The analysis was performed on an Inertsil ODS-4 column（4.6 mm×150 mm, 5 μm） with gradient elution. The mobile phases were 0.5% aqueous phosphoric acid and acetonitrile. The detection wavelength was 360 nm. The eight marker flavonoids were separated well with good linearity（r20.9991）, precision, stability and repeatability. The recovery rate was 95.58%–102.65%. Cluster analysis was employed to analyze 28 batches of samples. The result indicated that this method provides an efficient way to perform quality control as well as a scientific rationale for the Geo-authentication of Polygonum aviculare L.展开更多
In the present study,we aimed to investigate the effects of Polygonum hydropiper aqueous extract(PHE)on the expression levels of inflammatory cytokines and cytochrome P450(CYPs)in mice with E.coli-induced diarrhea.BAL...In the present study,we aimed to investigate the effects of Polygonum hydropiper aqueous extract(PHE)on the expression levels of inflammatory cytokines and cytochrome P450(CYPs)in mice with E.coli-induced diarrhea.BALB/c mice were randomly divided into the control group,model group,enrofloxacin-treated group,and two PHE-treated groups with different doses.The diarrhea model was established by intraperitoneal injection of enteropathogenic E.coli(EPEC)in mice.The enrofloxacin-treated group was given enrofloxacin at 5 mg/kg by intragastric gavage(i.g.)for 11 d.PHE-treated groups were given PHE at 5 g/kg and 10 g/kg by i.g.for 11 d.The histopathological characteristics of the duodenum and liver were observed by HE staining.The levels of inflammatory cytokines and CYPs in the duodenum and liver of mice were determined by ELISA.The m RNA and protein expressions of CYPs were determined by q RT-PCR and Western blotting analysis,respectively.The results showed that PHE could significantly alleviate the injury of the duodenum and liver induced by EPEC infection,reduce the contents and m RNA expressions of inflammatory cytokines,and regulate the m RNA and protein expressions of the major subtypes of CYPs.These findings indicated that PHE had an apparent therapeutic effect on EPEC-induced diarrhea,and its mechanism might be related to inhibition of inflammatory cytokines and regulation of CYPs.展开更多
基金Natural Science Foundation Project of Guangxi(2017GXNSFAA 198326)。
文摘[Objectives]To study the effect of total flavonoids extracted from Polygonum perfoliatum L.(TFP)on immune-mediated liver injury induced by bacillus Calmette-Guerin plus lipopolysaccharide(BCG+LPS)in mice,and to explore its action mechanism.[Methods]60 Kunming mice were divided into normal group,model group,control group(bifendate)and TFP low,medium and high dose groups according to random number table method,with 10 mice in each group.On the first day of modeling,mice were injected with 0.2 mL of BCG solution(12.5 mg/mL)through the tail vein,and on the eleventh day,0.2 mL of LPS(37.5μg/mL)were injected into the tail vein to prepare a mouse model of immune-mediated liver injury;from the first day of modeling,the normal group and the model group were administered intragastrically with the corresponding volume of distilled water,and the bifendate group and the TFP high,medium,and low dose groups were administered intragastrically with the corresponding doses once a day for 11 d.After the last time administration,fasting but giving water for 16 h,took blood from eyes,then collected the liver tissue.The levels of alanine transaminase(ALT)and aspartate transaminase(AST)in serum were detected by biochemical method;transforming growth factor-β1(TGF-β1),intercellular adhesion molecule-1(ICAM-1),interleukin-6(IL-6)and interleukin-1β(IL-1β)expression levels in liver tissue were detected by enzyme-linked immunosorbent assay(ELISA);phosphorylated protein tyrosine kinase JAK-2(p-JAK2),phosphorylated signal transducer and activator of transcription 3(p-STAT3)protein expression levels were detected by Western Blot method;the degree of liver tissue lesions was detected by HE staining.[Results]Compared with the model group,the levels of ALT and AST in the serum of mice in each dose group of TFP(high dose 600 mg/kg,medium dose 400 mg/kg,and low dose 200 mg/kg)were reduced,and the activities of T-SOD and GSH-Px were increased;the content or expression ofβ1,ICAM-1,IL-6,IL-1βdecreased,and the expression of p-JAK2 and p-STAT3 protein decreased;pathological sections showed that the degree of inflammatory necrosis and the degree of lesions in the liver tissues of each dose group of TFP were reduced by varying degrees.[Conclusions]TFP has a protective effect on BCG+LPS-induced immune-mediated liver injury in mice.The mechanism may be related to regulating the phosphorylation level of JAK2 and inhibiting the inflammatory reaction,thereby regulating the TGF-β1/STAT3 signaling pathway and improving the immune-mediated liver injury.
基金Study of Nature of Geo-authentic Crude Drug("9 73"State Key Project,Grant No.2006CB504700)
文摘A reliable high performance liquid chromatography method was developed for the quality evaluation of Polygonum aviculare L. Eight marker flavonoids were identified and simultaneously quantified, which included myricitrin, hyperoside, galuteolin, avicularin, quercitrin, quercetin, luteolin, and kaempferol. The analysis was performed on an Inertsil ODS-4 column（4.6 mm×150 mm, 5 μm） with gradient elution. The mobile phases were 0.5% aqueous phosphoric acid and acetonitrile. The detection wavelength was 360 nm. The eight marker flavonoids were separated well with good linearity（r20.9991）, precision, stability and repeatability. The recovery rate was 95.58%–102.65%. Cluster analysis was employed to analyze 28 batches of samples. The result indicated that this method provides an efficient way to perform quality control as well as a scientific rationale for the Geo-authentication of Polygonum aviculare L.
基金Key Project at Central Government Level:The ability establishment of sustainable use for valuable Chinese medicine resources(Grant No.2060302-2004-03)。
文摘In the present study,we aimed to investigate the effects of Polygonum hydropiper aqueous extract(PHE)on the expression levels of inflammatory cytokines and cytochrome P450(CYPs)in mice with E.coli-induced diarrhea.BALB/c mice were randomly divided into the control group,model group,enrofloxacin-treated group,and two PHE-treated groups with different doses.The diarrhea model was established by intraperitoneal injection of enteropathogenic E.coli(EPEC)in mice.The enrofloxacin-treated group was given enrofloxacin at 5 mg/kg by intragastric gavage(i.g.)for 11 d.PHE-treated groups were given PHE at 5 g/kg and 10 g/kg by i.g.for 11 d.The histopathological characteristics of the duodenum and liver were observed by HE staining.The levels of inflammatory cytokines and CYPs in the duodenum and liver of mice were determined by ELISA.The m RNA and protein expressions of CYPs were determined by q RT-PCR and Western blotting analysis,respectively.The results showed that PHE could significantly alleviate the injury of the duodenum and liver induced by EPEC infection,reduce the contents and m RNA expressions of inflammatory cytokines,and regulate the m RNA and protein expressions of the major subtypes of CYPs.These findings indicated that PHE had an apparent therapeutic effect on EPEC-induced diarrhea,and its mechanism might be related to inhibition of inflammatory cytokines and regulation of CYPs.
