[Objective] This study aimed to select SSR molecular markers linked to flesh color around the stone of Prunus persica （L.） Batsch. [Method] P. persica （L.） Batsch varieties Chongyanghong and Yanhong were used as p...[Objective] This study aimed to select SSR molecular markers linked to flesh color around the stone of Prunus persica （L.） Batsch. [Method] P. persica （L.） Batsch varieties Chongyanghong and Yanhong were used as parents to construct F1 orthogonal group. A total of 138 FI individuals were selected as experimental materi- als for construction of color around the stone gene pool （B1） and non-color around the stone gene pool （B2） by using bulked segregant analysis （BSA） method, molec- ular markers linked to the flesh color around the stone of P. persica （L.） Batsch were selected with SSR molecular marker technology. [Result] After selection with 256 pairs of SSR primers, three pairs of molecular markers linked to the gene con- trolling flesh color around the stone of P. persica （L.） Batsch were selected （UDP96- 003, ch04g09 and UDP97-402）. In addition, genetic distances between the three molecular markers and the gene controlling flesh color around the stone of P. persi- ca （L.） Batsch were calculated, which were 16.7, 10.1 and 17.0 cM, respectively. [Conclusion] This study laid the foundation for further selection of co-dominant molecular markers with closer genetic distance.展开更多
Sweet potato is a multifunctional root crop and a source of food with many essential nutrients and bioactive compounds.Variations in the flesh color of the diverse sweet potato varieties are attributed to the differen...Sweet potato is a multifunctional root crop and a source of food with many essential nutrients and bioactive compounds.Variations in the flesh color of the diverse sweet potato varieties are attributed to the different phytochemicals and natural pigments they produce.Among them,carotenoids and anthocyanins are the main pigments known for their antioxidant properties which provide a host of health benefits,hence,regarded as a major component of the human diet.In this review,we provide an overview of the major pigments in sweet potato with much emphasis on their biosynthesis,functions,and regulatory control.More-over,current findings on the molecular mechanisms underlying the biosynthesis and accumulation of carotenoids and anthocyanins in sweet potato are discussed.Insights into the composition,biosynthesis,and regulatory control of these major pigments will further advance the biofortification of sweet potato and provide a reference for breeding carotenoid-and anthocyanin-rich varieties.展开更多
The color of watermelon flesh is an important trait determined by a series of carotenoids.Herein,we used Cream of Saskatchewan(pale yellow flesh)and PI 186490(white flesh)as parental materials for an F2 segregation an...The color of watermelon flesh is an important trait determined by a series of carotenoids.Herein,we used Cream of Saskatchewan(pale yellow flesh)and PI 186490(white flesh)as parental materials for an F2 segregation and initial mapping using the bulked segregant analysis sequencing(BSA-seq)strategy.The BSA results revealed a flesh colorrelated QTL that spans approximately 2.45 Mb on chromosome 6.This region was preliminarily positioned in a 382-kb segment,and then narrowed down into a 66.8-kb segment with 1260 F2 individuals.A total of nine candidate genes were in the fine mapping interval,but only Cla007528(encoding chlorophyllase)had non-synonymous mutations and was significantly expressed between the parental materials throughout flesh development.We also checked the expression patterns of the carotenoid metabolic pathway genes based on RNA-seq data and qRT-PCR validation.Three genes in the xanthophyll cycle(ClCHYB,ClNCED-1 and ClNCED-7)exhibited differential expression patterns between the two parental lines at different flesh color formation stages.ClPSY1,ClPDS,ClZDS,ClCHXE,ClCRTISO and ClLCYB also exhibited clearly different expression patterns accompanied by carotenoid accumulation.展开更多
Fruit sugar and acid content were determined in a group of hybrids from one family,including 22 peach and 85 nectarine genotypes, or 75 white-fleshed and 32 yellow-fleshedgenotypes. Sucrose accounted for about 65% of ...Fruit sugar and acid content were determined in a group of hybrids from one family,including 22 peach and 85 nectarine genotypes, or 75 white-fleshed and 32 yellow-fleshedgenotypes. Sucrose accounted for about 65% of the total sugar, and malate for about 50%of the total acid. Mean sucrose, fructose, sorbitol, total sugar, soluble solid andquinate content were significantly higher in nectarines than in peaches, while nosignificant difference was found in mean malate, citrate and total acid content betweennectarines and peaches. Shikimate content was very low and ranged from 0.011-0.046 meq100 g-1 FW in all genotypes studied. Flesh color did not affect sugar and acid content.Glucose and fructose had a close positive correlation (r =0.63***). Malate had positivecorrelations with sucrose, sorbitol, total sugar and soluble solid content. The correlationbetween citrate and sugars were not significant. Glucose, fructose, sorbitol and malatecontent seemed to be higher in late-maturing genotypes. Sucrose, total sugar and totalacid content had a parabolic relationship with developmental period (from full bloomingdate to maturity date).展开更多
Mesocarp color is an important agronomic trait of Cucurbita maxima and is determined mainly by the contents and compositions of the carotenoids.The two inbred lines with significant differences in fruit flesh color we...Mesocarp color is an important agronomic trait of Cucurbita maxima and is determined mainly by the contents and compositions of the carotenoids.The two inbred lines with significant differences in fruit flesh color were used in the study,the orange’312-1’and white’98-2’.Changes in seven carotenoid contents and compositions in the flesh of fruit produced by inbred lines’312-1’and’98-2’were analyzed during fruit development.The expression of eight key carotenoid biosynthesis genes in the fruit flesh were investigated during five fruit development stage in two inbred lines.As the flesh color intensified,the orange flesh color of’312-1’was determined mainly by the increased contents of lutein,β-carotene and zeaxanthin and the lack of carotenoid accumulation led to the formation of white flesh in’98-2’fruit.The expression of the LCY-e and CHYb genes was significantly stronger in’312-1’than in’98-2’,and their expression was strongly correlated with lutein andβ-carotene contents during fruit development.This study provides a deep understanding of the molecular mechanism of carotenoid biosynthesis in fruit flesh and provides a basis for additional studies on the highly refined improvement of squash quality.展开更多
supported by the Key Laboratory Program of the Ministry of Agriculture of China （2013JCYJ-004） and its supplementary items （2015JSCX-036, 2015LWJJ-010）;the Program of Modern Agriculture Technology Innovation and D...supported by the Key Laboratory Program of the Ministry of Agriculture of China （2013JCYJ-004） and its supplementary items （2015JSCX-036, 2015LWJJ-010）;the Program of Modern Agriculture Technology Innovation and Demonstration of Provincial Finance Department, China （2014CXSF-015）展开更多
To compare the flesh quality of large yellow croaker cultured with three different modes, enclosure culture(EC), cage culture with feeding trash fish(CCF) and cage culture with feeding formula diet(CCD), thirty six fi...To compare the flesh quality of large yellow croaker cultured with three different modes, enclosure culture(EC), cage culture with feeding trash fish(CCF) and cage culture with feeding formula diet(CCD), thirty six fish individuals of EC(484.6 ± 79.8 g), CCF(432.7 ± 87.9 g) and CCD(416.9 ± 49.5 g) were sampled to measure body color, flesh water holding capacity, flesh proximate composition, amino acids and fatty acids composition, and collagen and inosinic acid contents. The analysis showed that there was no significant difference in the yellowness of skin and the contents of crude protein and inosinic acid between CCD and EC(P > 0.05), and the two modes showed higher values than CCF(P < 0.05). Most of the amino acids(AA) in flesh were higher in EC than in CCF(P < 0.05), but CCD showed no difference in AA composition from the other two modes(P > 0.05). EC and CCD had higher contents of collagen, free AA and lower drip loss than CC(P < 0.05). In polyunsaturated fatty acids, EC showed significantly higher levels of C20:5 n-3 and C22:6 n-3 than CCF and CCD(P < 0.05), but CCD had significantly higher C18:2 n-6 than CCF and EC(P < 0.05). In conclusion, the flesh quality of CCD fish is similar to EC fish, and these two modes have better flesh quality than CCF.展开更多
基金Supported by Fund of Hebei Academy of Agriculture and Forestry Sciences(A06120203)~~
文摘[Objective] This study aimed to select SSR molecular markers linked to flesh color around the stone of Prunus persica （L.） Batsch. [Method] P. persica （L.） Batsch varieties Chongyanghong and Yanhong were used as parents to construct F1 orthogonal group. A total of 138 FI individuals were selected as experimental materi- als for construction of color around the stone gene pool （B1） and non-color around the stone gene pool （B2） by using bulked segregant analysis （BSA） method, molec- ular markers linked to the flesh color around the stone of P. persica （L.） Batsch were selected with SSR molecular marker technology. [Result] After selection with 256 pairs of SSR primers, three pairs of molecular markers linked to the gene con- trolling flesh color around the stone of P. persica （L.） Batsch were selected （UDP96- 003, ch04g09 and UDP97-402）. In addition, genetic distances between the three molecular markers and the gene controlling flesh color around the stone of P. persi- ca （L.） Batsch were calculated, which were 16.7, 10.1 and 17.0 cM, respectively. [Conclusion] This study laid the foundation for further selection of co-dominant molecular markers with closer genetic distance.
基金This study was supported by the NSFC-Guangdong Natural Science Foundation Joint Project(U1701234)Strategic Leading Science&Technology Programme(XDA13020604)Program for Scientific Research Start-up Funds of Guangdong Ocean University,and Studies on Resistance Resources and Molecular Mechanisms of Sweet potato Weevil in South China(U1701234).
文摘Sweet potato is a multifunctional root crop and a source of food with many essential nutrients and bioactive compounds.Variations in the flesh color of the diverse sweet potato varieties are attributed to the different phytochemicals and natural pigments they produce.Among them,carotenoids and anthocyanins are the main pigments known for their antioxidant properties which provide a host of health benefits,hence,regarded as a major component of the human diet.In this review,we provide an overview of the major pigments in sweet potato with much emphasis on their biosynthesis,functions,and regulatory control.More-over,current findings on the molecular mechanisms underlying the biosynthesis and accumulation of carotenoids and anthocyanins in sweet potato are discussed.Insights into the composition,biosynthesis,and regulatory control of these major pigments will further advance the biofortification of sweet potato and provide a reference for breeding carotenoid-and anthocyanin-rich varieties.
基金This work was supported by the National Natural Science Foundation of China(31601775)the Project of China Postdoctoral Science Foundation(2017M611345)+1 种基金the earmarked fund for China Agriculture Research System of MOF and MARA(CARS-25)the Natural Science Foundation of Heilongjiang Province,China(C2017034).
文摘The color of watermelon flesh is an important trait determined by a series of carotenoids.Herein,we used Cream of Saskatchewan(pale yellow flesh)and PI 186490(white flesh)as parental materials for an F2 segregation and initial mapping using the bulked segregant analysis sequencing(BSA-seq)strategy.The BSA results revealed a flesh colorrelated QTL that spans approximately 2.45 Mb on chromosome 6.This region was preliminarily positioned in a 382-kb segment,and then narrowed down into a 66.8-kb segment with 1260 F2 individuals.A total of nine candidate genes were in the fine mapping interval,but only Cla007528(encoding chlorophyllase)had non-synonymous mutations and was significantly expressed between the parental materials throughout flesh development.We also checked the expression patterns of the carotenoid metabolic pathway genes based on RNA-seq data and qRT-PCR validation.Three genes in the xanthophyll cycle(ClCHYB,ClNCED-1 and ClNCED-7)exhibited differential expression patterns between the two parental lines at different flesh color formation stages.ClPSY1,ClPDS,ClZDS,ClCHXE,ClCRTISO and ClLCYB also exhibited clearly different expression patterns accompanied by carotenoid accumulation.
基金supported by funding from Service of Culture and Science Cooperation of French Embassy in China,the French National Institute for Agricultural Research(INRA)and Knowledge Innovation Program of the Chinese Academy of Sciences(KSCX2-SW-123)
文摘Fruit sugar and acid content were determined in a group of hybrids from one family,including 22 peach and 85 nectarine genotypes, or 75 white-fleshed and 32 yellow-fleshedgenotypes. Sucrose accounted for about 65% of the total sugar, and malate for about 50%of the total acid. Mean sucrose, fructose, sorbitol, total sugar, soluble solid andquinate content were significantly higher in nectarines than in peaches, while nosignificant difference was found in mean malate, citrate and total acid content betweennectarines and peaches. Shikimate content was very low and ranged from 0.011-0.046 meq100 g-1 FW in all genotypes studied. Flesh color did not affect sugar and acid content.Glucose and fructose had a close positive correlation (r =0.63***). Malate had positivecorrelations with sucrose, sorbitol, total sugar and soluble solid content. The correlationbetween citrate and sugars were not significant. Glucose, fructose, sorbitol and malatecontent seemed to be higher in late-maturing genotypes. Sucrose, total sugar and totalacid content had a parabolic relationship with developmental period (from full bloomingdate to maturity date).
基金the National Key Research and Development Program of China(Grant No.2018YFD0100706)。
文摘Mesocarp color is an important agronomic trait of Cucurbita maxima and is determined mainly by the contents and compositions of the carotenoids.The two inbred lines with significant differences in fruit flesh color were used in the study,the orange’312-1’and white’98-2’.Changes in seven carotenoid contents and compositions in the flesh of fruit produced by inbred lines’312-1’and’98-2’were analyzed during fruit development.The expression of eight key carotenoid biosynthesis genes in the fruit flesh were investigated during five fruit development stage in two inbred lines.As the flesh color intensified,the orange flesh color of’312-1’was determined mainly by the increased contents of lutein,β-carotene and zeaxanthin and the lack of carotenoid accumulation led to the formation of white flesh in’98-2’fruit.The expression of the LCY-e and CHYb genes was significantly stronger in’312-1’than in’98-2’,and their expression was strongly correlated with lutein andβ-carotene contents during fruit development.This study provides a deep understanding of the molecular mechanism of carotenoid biosynthesis in fruit flesh and provides a basis for additional studies on the highly refined improvement of squash quality.
基金supported by the Key Laboratory Program of the Ministry of Agriculture of China (2013JCYJ-004) and its supplementary items (2015JSCX-036, 2015LWJJ-010)the Program of Modern Agriculture Technology Innovation and Demonstration of Provincial Finance Department, China (2014CXSF-015)
文摘supported by the Key Laboratory Program of the Ministry of Agriculture of China （2013JCYJ-004） and its supplementary items （2015JSCX-036, 2015LWJJ-010）;the Program of Modern Agriculture Technology Innovation and Demonstration of Provincial Finance Department, China （2014CXSF-015）
基金supported by the Key Project of Science & Technology Promoting Agriculture (Shanghai municipal agricultural commission): Research of highlyefficient and ecologically formula feeds for large yellow croaker and groupers (No.2013-5-8)Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding (No.ZF1206)
文摘To compare the flesh quality of large yellow croaker cultured with three different modes, enclosure culture(EC), cage culture with feeding trash fish(CCF) and cage culture with feeding formula diet(CCD), thirty six fish individuals of EC(484.6 ± 79.8 g), CCF(432.7 ± 87.9 g) and CCD(416.9 ± 49.5 g) were sampled to measure body color, flesh water holding capacity, flesh proximate composition, amino acids and fatty acids composition, and collagen and inosinic acid contents. The analysis showed that there was no significant difference in the yellowness of skin and the contents of crude protein and inosinic acid between CCD and EC(P > 0.05), and the two modes showed higher values than CCF(P < 0.05). Most of the amino acids(AA) in flesh were higher in EC than in CCF(P < 0.05), but CCD showed no difference in AA composition from the other two modes(P > 0.05). EC and CCD had higher contents of collagen, free AA and lower drip loss than CC(P < 0.05). In polyunsaturated fatty acids, EC showed significantly higher levels of C20:5 n-3 and C22:6 n-3 than CCF and CCD(P < 0.05), but CCD had significantly higher C18:2 n-6 than CCF and EC(P < 0.05). In conclusion, the flesh quality of CCD fish is similar to EC fish, and these two modes have better flesh quality than CCF.
