THE INFLUENCE OF FERTILIZERS, NaHCO_3,SOIL TYPE ON CHEMISTRY OF FLOODED SOILS AND RICE PLANT GROWTH

This research was conducted to study the influence of NaHCO3and fertilizer treatments on flooded soils tothe chemistry of soil and rice plant growth.The experiment was done in a greenhouse using a split-split plot design ofthree factors: two types of soil,three doses of fertilizers and three levels of NaHCO3with three replications.The firstfactor was two kind of soils Fluvaquent and Calciquert,the factor was without nutrient(blank),macro nutrients,andcompletely nutrients(second macro and micro nutrients),and the third factor was 0 mg/kg,200 mg/kg and 400 mg/kgof NaHCO3or equivalent with 0 mg,1 200 mg and 2 400 mg NaHCO3per pot.Each experimental unit used a pot of 10L capacity and filled with soil mud equivalent with 6 kg of dried soil.After each of treatment combination mixed into themud soil,two plants of IR-32 variety were grown,and flooded at5 cm high accordingly with growth period.Parametersobserved were the growth of tillers,electrical conductivity(E.C.) and pH.The experiment results showed that the treatment of NaHCO3increased the soluble salt content(E.C.) and soilacidity(pH) during flooding on Calciquerts and Fluvaquents.The content of soluble salt decreased to 2.0 mmhos/cm,while pHon Calciquert decreased towards the neutral pH.Anyhowthe pHon Fluvaquents increased approach to neutral.The treatments of NaHCO3inhibited the tiller growth,either macro fertilizer and completely fertilizer increased thetillers,but did not improve the negative effect of alkalinity due to of NaHCOtreatment.

Influences of Quinclorac on Culturable Microorganisms and Soil Respiration in Flooded Paddy Soil

Objective To investigate the potential effects of herbicide quinclorac (3,7-dichloro-8-quinoline-carboxylic) on the culturable microorganisms in flooded paddy soil. Methods Total soil aerobic bacteria, actinomycetes and fungi were counted by a 10-fold serial dilution plate technique. Numbers of anaerobic fermentative bacteria (AFB), denitrifying bacteria (DNB) and hydrogen-producing acetogenic bacteria (HPAB) were numerated by three-tube anaerobic most-probable-number (MPN) methods with anaerobic liquid enrichment media. The number of methanogenic bacteria (MB) and nitrogen-fixing bacteria (NFB) was determined by the rolling tube method in triplicate. Soil respiration was monitored by a 102G-type gas chromatography with a stainless steel column filled with GDX-104 and a thermal conductivity detector. Results Quinclorac concentration was an important factor affecting the populations of various culturable microorganisms. There were some significant differences in the aerobic heterotrophic bacteria. AFB and DNB between soils were supplemented with quinclorac and non-quinclorac at the early stage of incubation, but none of them was persistent. The number of fungi and DNB was increased in soil samples treated by lower than 1.33μg·g-1 dried soil, while the CFU of fungi and HPAB was inhibited in soil samples treated by higher than 1.33μg·g-1 dried soil. The population of actinomycete declined in negative proportion to the concentrations of quinclorac applied after 4 days. However, application of quinclorac greatly stimulated the growth of AFB and NFB. MB was more sensitive to quinclorac than the others, and the three soil samples with concentrations higher than 1 μg·g-1 dried soil declined significantly to less than 40% of that in the control, but the number of samples with lower concentrations of quinclorac was nearly equal to that in the control at the end of experiments. Conclusion Quinclorac is safe to the soil microorganisms when applied at normal concentrations (0.67μg·g-1).

Bamboo biochar greater enhanced Cd/Zn accumulation in Salix psammophila under non-flooded soil compared with flooded

As a metal immobilizer,biochar can be used to remediate contaminated soil.Biochar’s effect on the phytoremediation process in flooded conditions under a scenario of increasing flooding frequency as global climate change is not well understood.This study investigated bamboo biochar(BBC)effects on growth and metal accumulation of Salix in multi-metal contaminated soil under non-flooded versus flooded conditions.Salix cuttings were cultivated in pots with severely contaminated soil by Cd and Zn,for 120 days,with four treatments including non-flooded treatment,flooded treatment,non-flooded with 3%BBC application(BBC/soil,w/w),and flooded with 3%BBC addition.BBC,flooding,and BBC×flooding significantly decreased the bioavailability of metals in soils(P<0.05).The BBC addition markedly stimulated Cd concentration in leaves under non-flooded(94.20%)and flooded conditions(32.73%)but showed little effect on roots.The BBC significantly boosted Cd and Zn transport from roots to aboveground parts by 68.85%and 102.27%compared with no BBC amendment under non-flooded treatment,while showing insignificant changes under flooded treatment.Although the plant biomass was little affected,BBC significantly increased Cd and Zn accumulation in the whole plant by 52.53%and 28.52%under non-flooded while showing an insignificant impact under flooded conditions.Taken together,BBC enhanced the phytoremediation efficiency of Salix to Cd and Zn in severely polluted non-flooded soil,while flooding offset this effect.The results indicated the effects of BBC varied under different soil moisture,which should be considered in the biochar-assisted phytoremediation to variable and complex environments.

Flooding impact on the distribution of microbial tetraether lipids in paddy rice soil in China

Isoprenoid and branched glycerol dialkyl glycerol tetraethers （GDGTs） lipids were studied in flooded and non-flooded paddy soil in Wuhan, central China, to examine the response of the GDGTs distribution to the soil flooding. Samples were collected before and after the soil flooding in four specific months. Both core （CL） and intact polar （IPL） GDGTs were quantified. Increase in the abundance of archaeol and caldarchaeol may be indicative of the occurrence of methanogens in the flooded soil. A negative correlation was observed between the ratio of IPL branched GDGT-IIa to GDGT-Ia and the soil pH. The rise of the soil pH in the acid soil is known to be controlled by the redox conditions resulting from flooding. Thus, the branched GDGTs distribution may be controlled by the water content in the paddy soil. In addition, we suggest that the anoxic conditions resulting from flooding may also control the abundance of branched GDGTs relative to crenarchaeol, which in turn results in the increase of branched and isoprenoidal tetraethers （BIT） values, the index for the terrestrial input to the marine sediments.